BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,717,170	C→A	5.5%	L139L (CTC→CTA) ‡	ung →	uracil‑DNA‑glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,717,170	0	C	A	5.5%	452.1 / 11.0	128	L139L (CTC→CTA) ‡	ung	uracil‑DNA‑glycosylase
Reads supporting (aligned to +/- strand): ref base C (58/63); new base A (4/3); total (62/66)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

Only 100 of 128 total aligned reads displayed.

ATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGAAAGCACCGCAT  >  NC_000913/2717025‑2717314

atatGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCGGCCTCGGc                                                                                                                                               <  1:271270/149‑1 (MQ=255)

atatGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCGGCCTCGGc                                                                                                                                               >  2:271270/1‑149 (MQ=255)

   tGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTgg                                                                                                                                            <  1:671404/149‑1 (MQ=255)

     tataAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGa                                                                                                                                          >  4:852722/1‑149 (MQ=255)

      ataAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGaa                                                                                                                                         >  1:390541/1‑149 (MQ=255)

       taAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCGAGGCTggcgtg                                                                                                                                        <  4:511685/149‑5 (MQ=255)

        aaaGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCGAGGCTggcgtgg                                                                                                                                       <  1:664546/149‑6 (MQ=255)

          agagCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCt                                                                                                                                     >  1:469162/1‑149 (MQ=255)

          agagCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCt                                                                                                                                     <  3:119056/149‑1 (MQ=255)

           gagCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCtt                                                                                                                                    <  2:465985/149‑1 (MQ=255)

               tGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGCTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCAcc                                                                                                                                >  3:16606/1‑149 (MQ=255)

                     aTACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCGGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATaaa                                                                                                                          <  3:612176/149‑1 (MQ=255)

                     aTACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATaaa                                                                                                                          <  3:223041/149‑1 (MQ=255)

                         tATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCa                                                                                                                                  <  1:280295/137‑1 (MQ=255)

                         tATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGa                                                                                                                      >  2:661117/1‑149 (MQ=255)

                          aTTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGAt                                                                                                                     <  4:612279/149‑1 (MQ=255)

                               gggCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGcc                                                                                                                <  2:297166/149‑1 (MQ=255)

                                ggCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCt                                                                                                               <  2:645317/149‑1 (MQ=255)

                                   ttCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGAt                                                                                                            >  2:371471/1‑149 (MQ=255)

                                    tCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGAtt                                                                                                           <  1:843686/149‑1 (MQ=255)

                                      aCCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGcc                                                                                                                >  1:373029/1‑142 (MQ=255)

                                      aCCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGcc                                                                                                                <  2:373029/142‑1 (MQ=255)

                                       cccGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAAc                                                                                                        <  1:371471/149‑1 (MQ=255)

                                            ccTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCa                                                                                                   >  2:833307/1‑149 (MQ=255)

                                             cTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCAt                                                                                                  >  4:405856/1‑149 (MQ=255)

                                              tAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCt                                                                                                                                              <  2:393424/104‑1 (MQ=255)

                                               aaTCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATcg                                                                                                <  3:852722/149‑1 (MQ=255)

                                                aTCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATcgc                                                                                               >  2:633994/1‑149 (MQ=255)

                                                  cttGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGc                                                                                                                 >  1:889303/3‑129 (MQ=255)

                                                  cATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGc                                                                                                                 <  2:889303/129‑1 (MQ=255)

                                                   aTGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATaaa                                                                                                                          >  3:811695/1‑119 (MQ=255)

                                                   aTGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATaaa                                                                                                                          <  4:811695/119‑1 (MQ=255)

                                                   aTGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGaa                                                                                            <  2:251826/149‑1 (MQ=255)

                                                   aTGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGaa                                                                                            >  2:847087/1‑149 (MQ=255)

                                                       ttATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCg                                                                                        >  3:188935/1‑149 (MQ=255)

                                                         aTCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCgtg                                                                                      <  3:372708/149‑1 (MQ=255)

                                                            ttGAAAGCTGGGCGCGTCATGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAAGCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCgtggtg                                                                                   >  4:581878/1‑149 (MQ=255)

                                                              gAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGtt                                                                                 >  4:15419/1‑149 (MQ=255)

                                                              gAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGtt                                                                                 >  3:118097/1‑149 (MQ=255)

                                                              gAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTAACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGtt                                                                                 <  4:458775/149‑1 (MQ=255)

                                                               aaaGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGttt                                                                                <  2:599096/149‑1 (MQ=255)

                                                                     gggCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTttgttg                                                                          >  1:392987/1‑149 (MQ=255)

                                                                        cgcgTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTgg                                                                       <  1:847087/149‑1 (MQ=255)

                                                                           gTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGCTAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGa                                                                    >  3:43167/1‑149 (MQ=255)

                                                                              aGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCg                                                                 >  3:491916/1‑149 (MQ=255)

                                                                               gggCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGc                                                                <  1:479923/149‑1 (MQ=255)

                                                                               gggCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGc                                                                <  2:469162/149‑1 (MQ=255)

                                                                                ggCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCa                                                               >  2:410862/1‑149 (MQ=255)

                                                                                  cGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATg                                                             <  2:770377/149‑1 (MQ=255)

                                                                                    ttCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATgcg                                                           >  1:533668/1‑149 (MQ=255)

                                                                                         cTACTCAATCCTGTGTTGGCTGCGCGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGCCTGGGAAACAGTCCCCTATACAGTGTCCAGGCTAATGATCCAGCATGGCGTAGGCGTGGTGTTTTTGTTGTTGGTATCCCATGCGCaaaa                                                      <  2:119381/149‑1 (MQ=255)

                                                                                         cTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTGCCATGCCAGCCTCGGCTGGGAAACCTTCACCGACAAAGTGATCAGCCTGATTAGCCAGCATCGCGAAGGCGTGGTGTTTATGTTGTGGGGATCGCATGCGCacaa                                                      <  4:575027/149‑1 (MQ=255)

                                                                                         cTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCaaaa                                                      <  1:833307/149‑1 (MQ=255)

                                                                                          tACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAaaag                                                     >  2:589574/1‑149 (MQ=255)

                                                                                          tACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAaaag                                                     >  4:190092/1‑149 (MQ=255)

                                                                                            cTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAaaagaa                                                   >  2:62978/1‑149 (MQ=255)

                                                                                             tCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAaaagaaa                                                  <  1:800386/149‑1 (MQ=255)

                                                                                             tCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAaaagaaa                                                  <  3:743162/149‑1 (MQ=255)

                                                                                               aaTACTGGGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGAGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAgg                                                >  2:799149/1‑149 (MQ=255)

                                                                                                 tACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAgggg                                              >  2:597801/1‑149 (MQ=255)

                                                                                                   cTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCg                                            >  3:20165/1‑149 (MQ=255)

                                                                                                    tgtgTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGa                                           <  3:850183/149‑1 (MQ=255)

                                                                                                    tgtgTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGa                                           >  2:7485/1‑149 (MQ=255)

                                                                                                      tgtTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCaaaa                                                      <  4:269706/136‑1 (MQ=255)

                                                                                                      tgtTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCaaaa                                                      >  3:269706/1‑136 (MQ=255)

                                                                                                      tgtTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGAtt                                         >  4:627122/1‑149 (MQ=255)

                                                                                                            cGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGAt                                   <  3:552197/149‑1 (MQ=255)

                                                                                                             ggTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATa                                  <  2:321461/149‑1 (MQ=255)

                                                                                                              gTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATaa                                 <  1:488716/149‑1 (MQ=255)

                                                                                                              gTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATaa                                 <  4:118097/149‑1 (MQ=255)

                                                                                                              gTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATaa                                 <  1:633994/149‑1 (MQ=255)

                                                                                                               tACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAg                                <  3:190092/149‑1 (MQ=255)

                                                                                                                  gcgcAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAaaagaaa                                                  >  4:512863/1‑128 (MQ=255)

                                                                                                                    gcAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATa                                                                                                                            >  3:873806/1‑52 (MQ=255)

                                                                                                                    gcAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATa                                                                                                                            <  4:873806/52‑1 (MQ=255)

                                                                                                                    gcAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAggg                                               >  1:53022/1‑129 (MQ=255)

                                                                                                                    gcAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAggg                                               <  2:53022/129‑1 (MQ=255)

                                                                                                                    gcAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACg                           >  1:719598/1‑149 (MQ=255)

                                                                                                                        gTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCcat                       <  1:283746/149‑1 (MQ=255)

                                                                                                                        gTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCcat                       <  4:20165/149‑1 (MQ=255)

                                                                                                                         tCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCcatc                      >  4:474811/1‑149 (MQ=255)

                                                                                                                         tCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCcatc                      <  4:16606/149‑1 (MQ=255)

                                                                                                                           aGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGANCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCcatcat                    <  1:589574/149‑1 (MQ=255)

                                                                                                                             gcgcATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGt                  >  3:661622/1‑149 (MQ=255)

                                                                                                                             gcgcATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGt                  >  2:149141/1‑149 (MQ=255)

                                                                                                                              cgcATTCCCTCGACAGCCTCGGCTGGGAAGCCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGGGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTa                 <  3:627133/149‑1 (MQ=255)

                                                                                                                              cgcATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTa                 <  3:581878/149‑1 (MQ=255)

                                                                                                                               gcATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTAc                <  4:188935/149‑1 (MQ=255)

                                                                                                                                  ttCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGa             >  3:827047/1‑149 (MQ=255)

                                                                                                                                    cccACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGaaa           <  1:7485/149‑1 (MQ=255)

                                                                                                                                     gtttcccAGCCGAGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGAAAg          >  3:480732/6‑149 (MQ=255)

                                                                                                                                     ccACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGAAAg          >  3:497530/1‑149 (MQ=255)

                                                                                                                                     ccACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGAAAg          >  1:17651/1‑149 (MQ=255)

                                                                                                                                      tttcccAGCCGAGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATgc                                                            <  3:91891/94‑1 (MQ=255)

                                                                                                                                       ttcccAGCCGAGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGAAAGCa        >  3:138889/4‑149 (MQ=255)

                                                                                                                                        tcccAGCCGAGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGAAAGCAc       >  2:476281/3‑149 (MQ=255)

                                                                                                                                         gCCAGCCTCGGCTGGGAAACCTTCACCGATACAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGAAAGCAcc      <  4:491916/149‑1 (MQ=255)

                                                                                                                                         gCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGAAAGCAcc      <  2:826765/149‑1 (MQ=255)

                                                                                                                                         gCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGAAAGCAcc      >  2:439103/1‑149 (MQ=255)

                                                                                                                                          ccAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGAAAGCACCg     >  1:710088/1‑149 (MQ=255)

ATATGTATAAAGAGCTGGAAAATACTATTCCGGGCTTCACCCGCCCTAATCATGGTTATCTTGAAAGCTGGGCGCGTCAGGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCGCATGCGCAAAAGAAAGGGGCGATTATAGATAAGCAACGCCATCATGTACTGAAAGCACCGCAT  >  NC_000913/2717025‑2717314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`