Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 385,724 T→A 6.3% W165R (TGG→AGG)  tauA → taurine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913385,7240TA 6.3% 385.0 / 12.2 111W165R (TGG→AGG) tauAtaurine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (48/56);  new base A (4/3);  total (52/59)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 115 total aligned reads displayed.

CAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTG  >  NC_000913/385576‑385871
                                                                                                                                                    |                                                                                                                                                   
cAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCt                                                                                                                                                     <  4:541568/149‑1 (MQ=255)
 aaaCCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTg                                                                                                                                                    >  4:297579/1‑149 (MQ=255)
       gAAGATCTGATTGCCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGATGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCACGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCg                                                                                                                                              >  2:545381/1‑149 (MQ=255)
        aaGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCgg                                                                                                                                             <  3:195637/149‑1 (MQ=255)
        aaGATCTGATTGACAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCgg                                                                                                                                             <  3:532849/149‑1 (MQ=255)
         aGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCggg                                                                                                                                            <  3:297579/149‑1 (MQ=255)
         aGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCggg                                                                                                                                            <  2:233264/149‑1 (MQ=255)
         aGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCggg                                                                                                                                            <  2:737630/149‑1 (MQ=255)
         aGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCggg                                                                                                                                            <  3:838608/149‑1 (MQ=255)
          gATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCgggg                                                                                                                                           >  2:278709/1‑149 (MQ=255)
          gATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCgggg                                                                                                                                           >  2:854990/1‑149 (MQ=255)
          gATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCAGGCAGCgata                                                                                                                                           <  1:345223/149‑4 (MQ=255)
           aTCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGga                                                                                                                                          <  3:351959/149‑1 (MQ=255)
           aTCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGga                                                                                                                                          <  4:655075/149‑1 (MQ=255)
           aTCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGga                                                                                                                                          >  3:181443/1‑149 (MQ=255)
            tCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGgag                                                                                                                                         >  1:294719/1‑149 (MQ=255)
              tGATTGGCAAACGCAACGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGat                                                                                                                                       >  3:383289/1‑149 (MQ=255)
               gATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGata                                                                                                                                      >  1:621674/1‑149 (MQ=255)
               gATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGata                                                                                                                                      <  1:832836/149‑1 (MQ=255)
                aTTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGatat                                                                                                                                     <  4:859765/149‑1 (MQ=255)
                aTTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGatat                                                                                                                                     <  3:707750/149‑1 (MQ=255)
                    gCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCt                                                                                                                                                     >  4:892102/1‑129 (MQ=255)
                    gCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCt                                                                                                                                                     <  3:892102/129‑1 (MQ=255)
                    gCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGAt                                                                                                                                 <  3:155105/149‑1 (MQ=255)
                    gCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGAt                                                                                                                                 <  1:662045/149‑1 (MQ=255)
                    gCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGAt                                                                                                                                 <  2:128171/149‑1 (MQ=255)
                    gCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGAt                                                                                                                                 <  2:871174/149‑1 (MQ=255)
                      aaaCGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGga                                                                                                                                          >  1:442239/1‑138 (MQ=255)
                        aCGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTg                                                                                                                             >  1:662776/1‑149 (MQ=255)
                            aTCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGAt                                                                                                                                 <  2:658433/141‑1 (MQ=255)
                            aTCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGAt                                                                                                                                 >  1:658433/1‑141 (MQ=255)
                            aTCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTa                                                                                                                         <  3:436124/149‑1 (MQ=255)
                               gCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCAGGCAGCga                                                                                                                                             <  3:80950/126‑2 (MQ=255)
                               gCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCAGGCAGCga                                                                                                                                             >  4:80950/1‑125 (MQ=255)
                                 cGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGTTGCTTATGTCt                                                                                                                    <  3:540758/149‑1 (MQ=255)
                                 cGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCt                                                                                                                    <  1:553655/149‑1 (MQ=255)
                                 cGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCt                                                                                                                    <  2:287327/149‑1 (MQ=255)
                                  gTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTg                                                                                                                   <  1:870331/149‑1 (MQ=255)
                                   tACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTgg                                                                                                                  >  3:124251/1‑149 (MQ=255)
                                   tACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTgg                                                                                                                  <  4:579616/149‑1 (MQ=255)
                                        tttATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCAGGCAGCgat                                                                                                                                            >  1:833153/1‑116 (MQ=255)
                                        tttATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCAGGCAGCgat                                                                                                                                            <  2:833153/118‑3 (MQ=255)
                                               ccaccaCCCACTACAGCCTGCTGGCGGCACTGAAGCACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGtt                                                                                                      <  3:536234/149‑1 (MQ=255)
                                                caccacCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTa                                                                                                     >  3:3042/1‑149 (MQ=255)
                                                caccacCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTa                                                                                                     >  2:163139/1‑149 (MQ=255)
                                                      ccacTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCTCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGccc                                                                                               >  3:628538/1‑149 (MQ=255)
                                                      ccacTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGccc                                                                                               >  1:507822/1‑149 (MQ=255)
                                                      ccacTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGccc                                                                                               <  1:278709/149‑1 (MQ=255)
                                                          tccAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGTTAACCGccggtgc                                                                                           <  2:621674/147‑6 (MQ=255)
                                                          tACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGa                                                                                           >  3:231420/1‑149 (MQ=255)
                                                             aGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGaaaa                                                                                        <  1:222234/149‑1 (MQ=255)
                                                              gCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGaaaaa                                                                                       >  1:445108/1‑149 (MQ=255)
                                                                ctgctgGCGGCCCTGAAACCCTGGGGCATTAAACCCGGGCACGTGGAGACGGTGAACCTACCGCCGCCCGCGATTAGCGCTGCCTGGCAGCGGGGAGATCTTGATGGTGCTTATGTCTGGGCACAGGTGGTTAACGGCCTGGAAAAAgt                                                                                     <  2:351276/149‑2 (MQ=255)
                                                                 tgctgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGAc                                                                                    >  4:24379/1‑149 (MQ=255)
                                                                 tgctgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGAc                                                                                    <  2:739063/149‑1 (MQ=255)
                                                                  gctgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGACAAAGACg                                                                                   <  2:507822/149‑1 (MQ=255)
                                                                  gctgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACg                                                                                   <  4:467325/149‑1 (MQ=255)
                                                                  gctgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACg                                                                                   <  2:150431/149‑1 (MQ=255)
                                                                       cggcACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAg                                                                              <  1:403450/149‑1 (MQ=255)
                                                                        ggcACTGAAACATTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGGTGGTGCTTATGTCTGGGCACCGGCGGCTAACGCCCTGGAAAAAGAAGGCAAgg                                                                             >  3:526705/1‑149 (MQ=255)
                                                                             tGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTg                                                                        >  1:679438/1‑149 (MQ=255)
                                                                             tGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTg                                                                        >  4:360125/1‑149 (MQ=255)
                                                                               aaaCACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGa                                                                                     >  4:316974/1‑134 (MQ=255)
                                                                               aaaCACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGa                                                                                     <  3:316974/134‑1 (MQ=255)
                                                                                   acTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACcgg                                                                                                           <  4:602085/108‑1 (MQ=255)
                                                                                    cTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGAtt                                                                 <  2:679438/149‑1 (MQ=255)
                                                                                       gggCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGa                                                                   <  3:459999/144‑1 (MQ=255)
                                                                                       gggCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGa                                                                   >  4:459999/1‑144 (MQ=255)
                                                                                         gCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGaa                                                            <  1:890522/149‑1 (MQ=255)
                                                                                           aTTANACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACa                                                          >  1:279659/1‑149 (MQ=255)
                                                                                            ttAAACCCGGGCAAGTGGAGATTGTGTACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAg                                                         >  3:299475/1‑149 (MQ=255)
                                                                                            ttAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCGCTGGAAAAAGACGGCAAGGTGTTGAc                                                                      <  1:181351/136‑1 (MQ=255)
                                                                                            ttAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAg                                                         >  4:869408/1‑149 (MQ=255)
                                                                                            ttAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAg                                                         >  3:162628/1‑149 (MQ=255)
                                                                                            ttAAACCCGGGCAAGCGGAGATTGTGAACCTGCCGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                                      >  2:181351/1‑136 (MQ=255)
                                                                                             tAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAgg                                                        <  4:181443/149‑1 (MQ=255)
                                                                                                aCCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGccc                                                                                               >  2:310927/1‑107 (MQ=255)
                                                                                                  ccGGGCAAGTGGAGATTGTGAACCTGCAGCTGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCggg                                                   >  4:700720/1‑149 (MQ=255)
                                                                                                       cAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTg                                              >  2:696086/1‑149 (MQ=255)
                                                                                                          gTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTgggg                                           >  1:783497/1‑149 (MQ=255)
                                                                                                             gagaTTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGgcgc                                        >  1:138627/1‑149 (MQ=255)
                                                                                                             gagaTTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGCTTAACGCCTTGGAAAAATACGGCAAGGTGTTGACCGATTCTGAACAGATCGGGCAGTGGGgcgc                                        >  4:551307/1‑149 (MQ=255)
                                                                                                              agaTTGTGAACCTGCAGCCGCCCGCTATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACAGATTCTGAACAGGTCGGGCAGTGGGgcgcg                                       <  2:327480/149‑1 (MQ=255)
                                                                                                                    tgAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGAAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACg                                 >  2:372384/1‑149 (MQ=255)
                                                                                                                     gAACTTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCATCGc                                <  4:568183/149‑1 (MQ=255)
                                                                                                                     gAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATATCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGc                                <  1:201646/149‑1 (MQ=255)
                                                                                                                      aaCCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCt                               >  4:344263/1‑149 (MQ=255)
                                                                                                                       aCCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTg                              >  2:591449/1‑149 (MQ=255)
                                                                                                                       aCCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTg                              >  1:808613/1‑149 (MQ=255)
                                                                                                                         cTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGa                            <  3:360125/149‑1 (MQ=255)
                                                                                                                            cAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGt                         <  4:3042/149‑1 (MQ=255)
                                                                                                                              gccgccCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCt                       <  1:919025/149‑1 (MQ=255)
                                                                                                                                cgcccgcGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTgg                     <  1:591449/149‑1 (MQ=255)
                                                                                                                                cgcccgcGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTgg                     <  4:231420/149‑1 (MQ=255)
                                                                                                                                    cgcgATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGAc                                                                                    <  1:773618/82‑1 (MQ=255)
                                                                                                                                    cgcgATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGAc                                                                                    >  2:773618/1‑82 (MQ=255)
                                                                                                                                     gcgATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGggtggt                <  3:578239/149‑1 (MQ=255)
                                                                                                                                         tccccGCTGCCAGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTCAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTgcgc            >  2:769533/5‑149 (MQ=255)
                                                                                                                                            ccGCTGCCAGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCaaa         >  1:456934/2‑149 (MQ=255)
                                                                                                                                                  ccTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGAtttt   <  2:133223/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAAACCGGAAGATCTGATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTG  >  NC_000913/385576‑385871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: