Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,796,375 T→A 7.6% I138F (ATT→TTT)  ygaU ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,796,3750TA 7.6% 414.1 / 19.1 118I138F (ATT→TTT) ygaUuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (52/57);  new base A (5/4);  total (57/61)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 130 total aligned reads displayed.

CCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAACTGGCTGGCAGTGGCTGGTGTCGCCGTT  >  NC_000913/2796227‑2796523
                                                                                                                                                    |                                                                                                                                                    
cccTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAAt                                                                                                                                                      >  4:905037/1‑149 (MQ=255)
      cTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATc                                                                                                                                                <  4:765959/149‑1 (MQ=255)
      cTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATc                                                                                                                                                <  4:421755/149‑1 (MQ=255)
         cggcggTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAAATTTATCCgg                                                                                                                                             <  1:674603/149‑1 (MQ=255)
          ggcggTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCggg                                                                                                                                            <  1:332906/149‑1 (MQ=255)
             ggTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCGGATAAAATTTATCCggggcaa                                                                                                                                        <  3:640562/149‑5 (MQ=255)
                aCTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCttttt                                                                                                                                      <  2:499768/149‑1 (MQ=255)
                 cTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTa                                                                                                                                     <  2:103879/149‑1 (MQ=255)
                 cTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTa                                                                                                                                     >  2:391260/1‑149 (MQ=255)
                 cTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTa                                                                                                                                     <  3:402556/149‑1 (MQ=255)
                    gcgcGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCa                                                                                                                                  >  1:930447/1‑149 (MQ=255)
                            tACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTa                                                                                                                          <  3:552179/149‑1 (MQ=255)
                              ccGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTAtt                                                                                                                        <  3:857249/149‑1 (MQ=255)
                               ctcgcgcgcCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTc                                                                                                                       >  2:923990/3‑149 (MQ=255)
                               cgcgcgcgCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTc                                                                                                                       >  4:825762/1‑149 (MQ=255)
                                        cAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAg                                                                                                              >  3:327911/1‑149 (MQ=255)
                                         aGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGaa                                                                                                               >  1:885708/1‑147 (MQ=255)
                                          gTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGAt                                                                                                            >  1:430057/1‑149 (MQ=255)
                                           tGAGTTAATGCTCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCTGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGAtt                                                                                                           >  1:490567/1‑149 (MQ=255)
                                             aGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGAtttt                                                                                                         <  4:581762/149‑1 (MQ=255)
                                                tAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACGCTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTAtt                                                                                                      <  2:55058/149‑1 (MQ=255)
                                                tAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTAtt                                                                                                      <  2:814857/149‑1 (MQ=255)
                                                  aTGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGt                                                                                                    >  4:332167/1‑149 (MQ=255)
                                                    gcgcCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTAc                                                                                                  >  3:920782/1‑149 (MQ=255)
                                                    gcgcCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTAc                                                                                                  >  2:99456/1‑149 (MQ=255)
                                                       ccAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGa                                                                                               >  2:618639/1‑149 (MQ=255)
                                                       ccAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGa                                                                                               <  4:223434/149‑1 (MQ=255)
                                                       ccAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGa                                                                                               <  1:682123/149‑1 (MQ=255)
                                                        cAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGAt                                                                                              >  4:789995/1‑149 (MQ=255)
                                                           cATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAg                                                                                           <  3:332167/149‑1 (MQ=255)
                                                            aTTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGc                                                                                          <  1:793624/149‑1 (MQ=255)
                                                                     cGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTa                                                                                 <  4:920782/149‑1 (MQ=255)
                                                                      gCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGa                                                                                                             >  4:290226/1‑120 (MQ=255)
                                                                      gCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGa                                                                                                             <  3:290226/120‑1 (MQ=255)
                                                                       ccAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCg                                                                                                                 >  3:331475/1‑115 (MQ=255)
                                                                         aaTTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGAcc                                                                             >  3:412660/1‑149 (MQ=255)
                                                                            tCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGt                                                                          >  3:229217/1‑149 (MQ=255)
                                                                              ccATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGttt                                                                        <  1:497323/149‑1 (MQ=255)
                                                                              ccATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTCCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGttt                                                                        >  3:284865/1‑149 (MQ=255)
                                                                               cATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTg                                                                       >  3:343288/1‑149 (MQ=255)
                                                                               cATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTg                                                                       >  1:528025/1‑149 (MQ=255)
                                                                                   aTCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGaaa                                                                   >  3:913407/1‑149 (MQ=255)
                                                                                   aTCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTATGGaaa                                                                   <  2:663686/149‑1 (MQ=255)
                                                                                    tCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTa                                                                                                   <  4:353773/116‑1 (MQ=255)
                                                                                    tCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTa                                                                                                   >  3:353773/1‑116 (MQ=255)
                                                                                    tCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAAt                                                                  <  3:311669/149‑1 (MQ=255)
                                                                                    tCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAAt                                                                  >  1:271327/1‑149 (MQ=255)
                                                                                      cagcagGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCAGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATgg                                                                <  4:412660/149‑1 (MQ=255)
                                                                                        gcagGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTAcc                                                                                    >  2:799133/1‑127 (MQ=255)
                                                                                        gcagGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTAcc                                                                                    <  1:799133/127‑1 (MQ=255)
                                                                                        gcagGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCa                                                              >  4:412176/1‑149 (MQ=255)
                                                                                            gCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCa                                                          >  2:341595/1‑149 (MQ=255)
                                                                                              gATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTa                                                                                                                                     >  1:601611/1‑72 (MQ=255)
                                                                                              gATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCaga                                                        <  2:485441/149‑1 (MQ=255)
                                                                                              gATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGCTTGGAAATGGCACTCaga                                                        <  3:905037/149‑1 (MQ=255)
                                                                                               aTGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACACagag                                                       <  4:74651/149‑1 (MQ=255)
                                                                                                   ctggcAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTc                                                   <  3:783497/149‑1 (MQ=255)
                                                                                                   ctggcAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTc                                                   <  2:566276/149‑1 (MQ=255)
                                                                                                   ctggcAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTc                                                   <  2:646897/149‑1 (MQ=255)
                                                                                                    tggcAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGaaa                                                                   <  4:213679/132‑1 (MQ=255)
                                                                                                     ggcAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGc                                                 >  2:31682/1‑149 (MQ=255)
                                                                                                      gcAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCg                                                                                   >  1:227730/1‑114 (MQ=255)
                                                                                                      gcAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCg                                                                                   <  2:227730/114‑1 (MQ=255)
                                                                                                       cAATTAACTACTCTTCCTGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCa                                               <  2:490567/149‑1 (MQ=255)
                                                                                                       cAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCa                                               <  2:120333/149‑1 (MQ=255)
                                                                                                         aTTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCCGa                                             <  4:303668/149‑1 (MQ=255)
                                                                                                         aTTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGa                                             >  2:234097/1‑149 (MQ=255)
                                                                                                              ctactCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGttt                                                                        >  2:447762/1‑117 (MQ=255)
                                                                                                              ctactCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGttt                                                                        <  1:447762/117‑1 (MQ=255)
                                                                                                                actCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACg                                      >  3:872612/1‑149 (MQ=255)
                                                                                                                  tctTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGt                                    >  1:395739/1‑149 (MQ=255)
                                                                                                                   ctTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGta                                   <  4:343288/149‑1 (MQ=255)
                                                                                                                   ctTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGta                                   >  4:603891/1‑149 (MQ=255)
                                                                                                                   ctTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGta                                   <  1:175470/149‑1 (MQ=255)
                                                                                                                    ttCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTa                                                                                                   <  1:53865/84‑1 (MQ=255)
                                                                                                                    ttCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTa                                                                                                   >  2:53865/1‑84 (MQ=255)
                                                                                                                    ttCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGtat                                  >  1:810947/1‑149 (MQ=255)
                                                                                                                            tACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAActgg                          <  1:234097/149‑1 (MQ=255)
                                                                                                                               gCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAActggctg                       >  1:391116/1‑149 (MQ=255)
                                                                                                                                         gcccGGATAAAATTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAACTGGCTGGCAGTGGCTg             >  1:205423/2‑149 (MQ=255)
                                                                                                                                         ccccGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAACTGGCTGGCAGTGGCTg             <  2:810947/149‑1 (MQ=255)
                                                                                                                                         ccccGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAACTGGCTGGCAGTGGCTg             <  4:913407/149‑1 (MQ=255)
                                                                                                                                           ccGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAACTGGCTGGCAGTGGCTGgt           >  2:239237/1‑149 (MQ=255)
                                                                                                                                           ccGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAACTGGCTGGCAGTGGCTGgt           >  2:79655/1‑149 (MQ=255)
                                                                                                                                           ccGGATAAAATTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGc                                                 <  4:622805/111‑1 (MQ=255)
                                                                                                                                           ccGGATAAAATTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGc                                                 >  3:622805/1‑111 (MQ=255)
                                                                                                                                           ccGGATAAAATTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAACTGGCTGGCAGTGGCTGgt           >  4:737456/1‑149 (MQ=255)
                                                                                                                                           ccGGATAAAATTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAACTGGCTGGCAGTGGCTGgt           >  3:376808/1‑149 (MQ=255)
                                                                                                                                            cGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAACTGGCTGGCAGTGGCTGgtg          >  4:206417/1‑149 (MQ=255)
                                                                                                                                             ggATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAACTGGCTGGCAGTGGCTGgtgt         <  3:87806/149‑1 (MQ=255)
                                                                                                                                              gATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGAc                                            >  3:77558/1‑113 (MQ=255)
                                                                                                                                              gATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGAc                                            <  4:77558/113‑1 (MQ=255)
                                                                                                                                                   aTTTTATCCTGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAACTGGCTGACAGTGGCTGGTGTCGCCGt   <  3:780534/149‑1 (MQ=255)
                                                                                                                                                   aTTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAACTGGCTGGCAGTGGCTGGTGTCGCCGt   <  4:872612/149‑1 (MQ=255)
                                                                                                                                                    ttttATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCaga                                                        <  3:10974/95‑1 (MQ=255)
                                                                                                                                                    ttttATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCaga                                                        >  4:10974/1‑95 (MQ=255)
                                                                                                                                                    ttttATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTaa                                        <  4:335394/111‑1 (MQ=255)
                                                                                                                                                    ttttATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAAc                                       <  3:695833/112‑1 (MQ=255)
                                                                                                                                                    ttttATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAACTGGCTGGCAGTGGCTGGTGTCGCCGtt  <  3:603891/149‑1 (MQ=255)
                                                                                                                                                    ttttATCCGGGCTTTTTAGCATCGGTTTATTCGCATTGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTTGACAGACTTAACGGTCTAAAACTGGCTGGCAGTGGCTGGTGTCGACGtt  >  4:264782/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCAGAGTGTCGCCAGACTTAACGGTATAAAACTGGCTGGCAGTGGCTGGTGTCGCCGTT  >  NC_000913/2796227‑2796523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: