Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,172 T→C 7.6% T29A (ACG→GCG)  ttdR ← transcriptional activator of ttdABT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,1720TC 7.6% 332.1 / 13.5 105T29A (ACG→GCG) ttdRtranscriptional activator of ttdABT
Reads supporting (aligned to +/- strand):  ref base T (49/48);  new base C (3/5);  total (52/53)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.67e-01

Only 100 of 108 total aligned reads displayed.

TGAGGATTTCCAACGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCT  >  NC_000913/3206025‑3206315
                                                                                                                                                   |                                                                                                                                               
tGAGGATTTCCAACGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTc                                                                                                                                                <  3:477835/149‑1 (MQ=255)
tGAGGATTTCCAACGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTc                                                                                                                                                <  1:164859/149‑1 (MQ=255)
tGAGGATTTCCAACGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTc                                                                                                                                                >  1:936202/1‑149 (MQ=255)
tGAGGATTTCCAACGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTc                                                                                                                                                <  3:581340/149‑1 (MQ=255)
tGAGGATTTCCAACGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTc                                                                                                                                                <  2:667680/149‑1 (MQ=255)
 gAGGATTTCCAACGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGCcg                                                                                                                                               >  3:372632/1‑149 (MQ=255)
  aGGATTTCCAACGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGcgccgc                                                                                                                                              >  2:313485/1‑149 (MQ=255)
          cAACGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGCCGCAgcggcga                                                                                                                                      >  4:418619/1‑148 (MQ=255)
           aaCGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTgcggc                                                                                                                                     >  1:881633/1‑149 (MQ=255)
           aaCGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTgcggc                                                                                                                                     >  1:814576/1‑149 (MQ=255)
           aaCGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTgcggc                                                                                                                                     <  4:558796/149‑1 (MQ=255)
            aCGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTgcggcg                                                                                                                                    >  2:384420/1‑149 (MQ=255)
            aCGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTgcggcg                                                                                                                                    >  3:216233/1‑149 (MQ=255)
             cGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGCCGCAGCGGCGacgc                                                                                                                                   <  4:372632/149‑1 (MQ=255)
              gCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCt                                                                                                                                  >  1:230786/1‑149 (MQ=255)
              gCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCt                                                                                                                                  >  1:768775/1‑149 (MQ=255)
               cATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGCCGCAGCGGCGACGCtt                                                                                                                                 <  1:313485/149‑2 (MQ=255)
               cATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGCCGCAGCGGCGACGCtt                                                                                                                                 <  2:516075/149‑2 (MQ=255)
                  gTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTaaaa                                                                                                                              <  2:346584/149‑1 (MQ=255)
                          caacGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCt                                                                                                                      >  4:143658/1‑149 (MQ=255)
                               ttGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGaa                                                                                                                 <  4:322463/149‑1 (MQ=255)
                               ttGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGaa                                                                                                                 <  2:55074/149‑1 (MQ=255)
                               ttGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGaa                                                                                                                 <  4:215655/149‑1 (MQ=255)
                                tGGCCACTTTCAGTCAGCGCCACGCCGCGAGTGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAAc                                                                                                                <  2:265111/149‑1 (MQ=255)
                                 ggCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGACCg                                                                                                               >  4:846788/1‑149 (MQ=255)
                                 ggCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACg                                                                                                               >  1:377551/1‑149 (MQ=255)
                                  gCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGa                                                                                                              >  4:232120/1‑149 (MQ=255)
                                    cACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAACCTCCCGCTGTGAACGAtt                                                                                                            <  3:846344/149‑1 (MQ=255)
                                    cACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGCCGCAGCGGCGACGCttgg                                                                                                                               <  4:445150/130‑4 (MQ=255)
                                    cACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGCCGCAGCGGCGACGCttgg                                                                                                                               >  3:445150/1‑127 (MQ=255)
                                     aCTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGAttt                                                                                                           <  4:746763/149‑1 (MQ=255)
                                       tttCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTcgccgc                                                                                                                                           >  2:543077/1‑115 (MQ=255)
                                       tttCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTcgccgc                                                                                                                                           >  1:652171/1‑115 (MQ=255)
                                       tttCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTcgccgc                                                                                                                                           <  1:543077/115‑1 (MQ=255)
                                       tttCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTcgccgc                                                                                                                                           <  2:652171/115‑1 (MQ=255)
                                       tttCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGc                                                                                                                       <  4:514160/135‑1 (MQ=255)
                                       tttCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGc                                                                                                                       >  3:514160/1‑135 (MQ=255)
                                       tttCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCt                                                                                                         >  3:169059/1‑149 (MQ=255)
                                       tttCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCt                                                                                                         >  4:786174/1‑149 (MQ=255)
                                               agCGCCACGCCGCGAGCGGAGCGGATCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTAc                                                                                                 <  2:814576/149‑1 (MQ=255)
                                                     aCGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCTCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCaa                                                                                           <  1:302509/149‑1 (MQ=255)
                                                           cgAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCCAGTCttt                                                                                     <  4:159846/149‑1 (MQ=255)
                                                           cgAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCttt                                                                                     <  2:354486/149‑1 (MQ=255)
                                                           cgAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCttt                                                                                     <  3:143658/149‑1 (MQ=255)
                                                             agcggagcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTgg                                                                                   <  3:792840/149‑1 (MQ=255)
                                                                ggagcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTa                                                                                >  2:474555/1‑149 (MQ=255)
                                                                  agcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTaaa                                                                              <  3:226171/149‑1 (MQ=255)
                                                                  agcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTaaa                                                                              <  1:384420/149‑1 (MQ=255)
                                                                   gcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGATGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGGACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAg                                                                             <  4:266662/149‑1 (MQ=255)
                                                                      gTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGcc                                                                          <  1:699340/149‑1 (MQ=255)
                                                                       ttCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCa                                                                         <  2:53043/149‑1 (MQ=255)
                                                                            cagcGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAACACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTAt                                                                    >  3:162942/1‑149 (MQ=255)
                                                                               cGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCa                                                                 <  2:935680/149‑1 (MQ=255)
                                                                                   gttgCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCAt                                                             >  1:427210/1‑149 (MQ=255)
                                                                                     tgCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCg                                                           <  3:528911/149‑1 (MQ=255)
                                                                                       ccAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTg                                                                                    >  4:220258/1‑122 (MQ=255)
                                                                                       ccAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTg                                                                                    <  3:220258/122‑1 (MQ=255)
                                                                                       ccAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGtt                                                         >  3:13049/1‑149 (MQ=255)
                                                                                         aGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTaa                                                       >  2:102416/1‑149 (MQ=255)
                                                                                         aGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTaa                                                       >  1:848280/1‑149 (MQ=255)
                                                                                          gCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAAt                                                      >  1:13379/1‑149 (MQ=255)
                                                                                          gCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAAt                                                      <  1:124902/149‑1 (MQ=255)
                                                                                              aTTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATc                                                  >  1:153831/1‑149 (MQ=255)
                                                                                               ttttCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATcc                                                 >  1:403515/1‑149 (MQ=255)
                                                                                                  tCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCa                                              >  1:470389/1‑149 (MQ=255)
                                                                                                  tCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCa                                              >  2:517171/1‑149 (MQ=255)
                                                                                                   cAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAg                                             >  4:462976/1‑149 (MQ=255)
                                                                                                    aaGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGt                                            >  3:7317/1‑149 (MQ=255)
                                                                                                     aGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGtt                                           >  3:136479/1‑149 (MQ=255)
                                                                                                     aGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGtt                                           <  2:427210/149‑1 (MQ=255)
                                                                                                       gATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTtgt                                         >  4:732572/1‑149 (MQ=255)
                                                                                                          ttGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGAt                                      <  2:13379/149‑1 (MQ=255)
                                                                                                            ggATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATaa                                    >  1:380870/1‑149 (MQ=255)
                                                                                                            ggATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATaa                                    >  3:463576/1‑149 (MQ=255)
                                                                                                            ggATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATaa                                    <  2:780749/149‑1 (MQ=255)
                                                                                                             gATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAg                                   <  4:219987/149‑1 (MQ=255)
                                                                                                             gATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAg                                   >  1:607982/1‑149 (MQ=255)
                                                                                                              aTCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAgc                                  <  4:641596/149‑1 (MQ=255)
                                                                                                               tCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCa                                                                                                     <  2:741043/81‑1 (MQ=255)
                                                                                                               tCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCa                                                                                                     >  1:741043/1‑81 (MQ=255)
                                                                                                               tCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAgcg                                 >  4:517708/1‑149 (MQ=255)
                                                                                                                    ttGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAgtg                            >  1:512299/1‑149 (MQ=255)
                                                                                                                     tGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAgtgt                           <  3:216558/149‑1 (MQ=255)
                                                                                                                      ggTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTa                          >  3:502340/1‑149 (MQ=255)
                                                                                                                        tGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAtt                        >  4:239861/1‑149 (MQ=255)
                                                                                                                         gACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAttt                       <  3:232120/149‑1 (MQ=255)
                                                                                                                          aCAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAttt                       >  3:164848/1‑148 (MQ=255)
                                                                                                                          aCAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAttt                       <  4:164848/148‑1 (MQ=255)
                                                                                                                          aCAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCAGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGGAGTGTATTTc                      <  4:601771/149‑1 (MQ=255)
                                                                                                                            aaaTGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGc                    <  1:698722/149‑1 (MQ=255)
                                                                                                                            aaaTGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGc                    >  2:343236/1‑149 (MQ=255)
                                                                                                                              aTGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCaa                  >  2:847015/1‑149 (MQ=255)
                                                                                                                                    gCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACAtt            <  3:54613/149‑1 (MQ=255)
                                                                                                                                      gTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGc          <  2:848280/149‑1 (MQ=255)
                                                                                                                                           cccAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTc     >  4:17933/1‑149 (MQ=255)
                                                                                                                                            ccAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTaa                                                       <  3:91557/98‑1 (MQ=255)
                                                                                                                                            ccAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTcc    >  2:486987/1‑149 (MQ=255)
                                                                                                                                              aaGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCt  <  4:169059/149‑1 (MQ=255)
                                                                                                                                                   tCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTccc   >  2:22632/1‑143 (MQ=255)
                                                                                                                                                   tCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTccc   <  1:22632/143‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TGAGGATTTCCAACGCATGTTCGTAACAACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCT  >  NC_000913/3206025‑3206315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: