Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,246,562 C→A 6.3% intergenic (+40/‑90) exuT → / → exuR hexuronate transporter/hexuronate regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,246,5620CA 6.3% 431.5 / 9.9 123intergenic (+40/‑90)exuT/exuRhexuronate transporter/hexuronate regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (53/62);  new base A (5/3);  total (58/65)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCG  >  NC_000913/3246414‑3246710
                                                                                                                                                    |                                                                                                                                                    
ggCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTtc                                                                                                                                                      >  2:42110/1‑149 (MQ=255)
ggCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTtc                                                                                                                                                      >  2:393493/1‑149 (MQ=255)
ggCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTATCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTtc                                                                                                                                                      <  3:11391/149‑1 (MQ=255)
 gCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTtct                                                                                                                                                     >  1:81782/1‑149 (MQ=255)
   aGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCa                                                                                                                                                   >  4:359023/1‑149 (MQ=255)
   aGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCa                                                                                                                                                   <  4:335236/149‑1 (MQ=255)
      gtTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGTGGTGGCGCAGGAAACGCATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAggc                                                                                                                                                <  1:620889/149‑1 (MQ=255)
          gACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCt                                                                                                                                            <  2:380727/149‑1 (MQ=255)
           aCCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCtt                                                                                                                                           <  4:531279/149‑1 (MQ=255)
            ccTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCttt                                                                                                                                          >  2:434871/1‑149 (MQ=255)
             cTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCtttt                                                                                                                                         <  2:338908/149‑1 (MQ=255)
              tGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGTAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTGAGAAGGCGGCtttga                                                                                                                                        <  2:219315/149‑3 (MQ=255)
                ttAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtca                                                                                                                                      >  1:155815/1‑149 (MQ=255)
                ttAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTGAGAAGGCGGCtttgaa                                                                                                                                       >  3:43903/1‑145 (MQ=255)
                ttAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTGAGAAGGCGGCtttgaa                                                                                                                                       <  4:43903/148‑4 (MQ=255)
                      gcgcTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTg                                                                                                                                <  1:393493/149‑1 (MQ=255)
                        gcTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCg                                                                                                                              >  4:472459/1‑149 (MQ=255)
                                tCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGc                                                                                                                      >  3:70957/1‑149 (MQ=255)
                                  tGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTa                                                                                                                    >  1:13248/1‑149 (MQ=255)
                                       cGTGTTGCAGAACAAACCGGCGATTGAGGAGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTc                                                                                                               <  2:434662/149‑1 (MQ=255)
                                               aGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGt                                                                                                       >  2:119631/1‑149 (MQ=255)
                                               aGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGt                                                                                                       >  2:300336/1‑149 (MQ=255)
                                                gAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTg                                                                                                      <  3:809072/149‑1 (MQ=255)
                                                       ccGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtcatca                                                                                                                                   <  3:516067/113‑1 (MQ=255)
                                                       ccGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtcatca                                                                                                                                   >  4:516067/1‑113 (MQ=255)
                                                         ggCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACaa                                                                                             >  2:465034/1‑149 (MQ=255)
                                                          gCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACaaa                                                                                            <  1:465034/149‑1 (MQ=255)
                                                              ttGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAAtata                                                                                        <  2:715614/149‑1 (MQ=255)
                                                              ttGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAAtata                                                                                        <  3:359023/149‑1 (MQ=255)
                                                                gAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGt                                                                                      <  1:434871/149‑1 (MQ=255)
                                                                   gTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACt                                                                                                                                 >  1:305684/1‑103 (MQ=255)
                                                                   gTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACt                                                                                                                                 <  2:305684/103‑1 (MQ=255)
                                                                     ggCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGcc                                                                                 >  1:377257/1‑149 (MQ=255)
                                                                       cgcAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGt                                                                               <  1:300336/149‑1 (MQ=255)
                                                                        gcAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTa                                                                              >  3:424179/1‑149 (MQ=255)
                                                                        gcAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTa                                                                              >  4:748408/1‑149 (MQ=255)
                                                                        gcAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTa                                                                              >  3:144786/1‑149 (MQ=255)
                                                                        gcAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTa                                                                              <  2:61180/149‑1 (MQ=255)
                                                                             aaaCACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATg                                                                         >  1:142049/1‑149 (MQ=255)
                                                                               acacATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGcc                                                                       <  4:70957/149‑1 (MQ=255)
                                                                                cacaTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCt                                                                      <  2:23716/149‑1 (MQ=255)
                                                                                 acaTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTg                                                                     <  4:758595/149‑1 (MQ=255)
                                                                                 acaTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTg                                                                     <  4:52669/149‑1 (MQ=255)
                                                                                 acaTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTg                                                                     >  3:158128/1‑149 (MQ=255)
                                                                                   aTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGa                                                                   >  3:395846/1‑149 (MQ=255)
                                                                                   aTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGa                                                                   >  1:534013/1‑149 (MQ=255)
                                                                                        gATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCa                                                              >  2:797068/1‑149 (MQ=255)
                                                                                        gATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCa                                                              >  1:451698/1‑149 (MQ=255)
                                                                                         aTCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCat                                                             <  2:720619/149‑1 (MQ=255)
                                                                                          tCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAGTATAGTCTGCCGTATCATGCCTGGAGCGCata                                                            <  1:717392/149‑1 (MQ=255)
                                                                                          tCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCata                                                            <  4:333334/149‑1 (MQ=255)
                                                                                          tCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCata                                                            <  3:472459/149‑1 (MQ=255)
                                                                                             cgcaccgcaACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATgg                                                         >  2:594493/1‑149 (MQ=255)
                                                                                              gcaccgcaACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGa                                                        <  3:799344/149‑1 (MQ=255)
                                                                                                accgcaACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGaaa                                                      <  4:28147/149‑1 (MQ=255)
                                                                                                    caACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCAc                                                  <  1:435739/149‑1 (MQ=255)
                                                                                                     aaCATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACt                                                 <  1:712541/149‑1 (MQ=255)
                                                                                                      aCATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGcc                                                                                 <  1:572752/116‑1 (MQ=255)
                                                                                                      aCATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGcc                                                                                 >  2:572752/1‑116 (MQ=255)
                                                                                                        aTTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCt                                                                      <  3:923035/125‑1 (MQ=255)
                                                                                                         ttAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAAtata                                                                                        <  2:632099/106‑1 (MQ=255)
                                                                                                         ttAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAAtata                                                                                        >  1:632099/1‑106 (MQ=255)
                                                                                                           aaCGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGa                                                        >  1:386962/1‑136 (MQ=255)
                                                                                                           aaCGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGa                                                        <  2:386962/136‑1 (MQ=255)
                                                                                                            aCGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCAc                                          >  4:911199/1‑149 (MQ=255)
                                                                                                               tttCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGa                                                                   >  3:367648/1‑121 (MQ=255)
                                                                                                               tttCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGa                                                                   <  4:367648/121‑1 (MQ=255)
                                                                                                               tttCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATg                                                          <  1:796416/130‑1 (MQ=255)
                                                                                                               tttCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATg                                                          >  2:796416/1‑130 (MQ=255)
                                                                                                                   gtgtAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCAc                                                  <  4:648274/134‑1 (MQ=255)
                                                                                                                   gtgtAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCAc                                                  >  3:648274/1‑134 (MQ=255)
                                                                                                                      tAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTa                                <  2:451698/149‑1 (MQ=255)
                                                                                                                      tAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTa                                <  3:333561/149‑1 (MQ=255)
                                                                                                                          ttttAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGGCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATcaa                            >  3:798704/1‑149 (MQ=255)
                                                                                                                          ttttAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATcaa                            <  4:395846/149‑1 (MQ=255)
                                                                                                                          ttttAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATcaa                            >  1:651468/1‑149 (MQ=255)
                                                                                                                           tttAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATcaac                           <  3:849747/149‑1 (MQ=255)
                                                                                                                             tAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATcaacaa                         <  3:136640/149‑1 (MQ=255)
                                                                                                                                tttCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCa                                           <  3:326808/128‑1 (MQ=255)
                                                                                                                                tttCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCa                                           >  4:326808/1‑128 (MQ=255)
                                                                                                                                tttCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACtt                      <  1:119631/149‑1 (MQ=255)
                                                                                                                                   cccTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGcc                   <  4:867112/149‑1 (MQ=255)
                                                                                                                                     tgaaaaaGCCGCCTGAGAAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAAcc                                            >  4:211814/5‑122 (MQ=255)
                                                                                                                                        cAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGa              <  1:72393/149‑1 (MQ=255)
                                                                                                                                        cAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGa              >  2:594510/1‑149 (MQ=255)
                                                                                                                                         aaaGCCGCCTGAGAAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGAc             >  1:293722/1‑149 (MQ=255)
                                                                                                                                          aaGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGAcc            <  3:911199/149‑1 (MQ=255)
                                                                                                                                          aaGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGAcc            <  4:672961/149‑1 (MQ=255)
                                                                                                                                           tgacgccTCCTCAGGCGGCTTTTTAATCGCTGCGAGCAGAGCTAAACTCGCAAAAGTGGTATAACAGCTATAGTCTGCCGTATCATGCCTGGAGGGAATATGTAAATCACTGAAACACGCCGTTTATTTCAACAACTTGCCGCTGACCt           <  4:210532/146‑1 (MQ=255)
                                                                                                                                           aGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCt           >  4:161187/1‑149 (MQ=255)
                                                                                                                                           aGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCt           >  4:101156/1‑149 (MQ=255)
                                                                                                                                                ccTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCAt                                                                          >  3:652729/1‑81 (MQ=255)
                                                                                                                                                ccTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCAt                                                                          <  4:652729/81‑1 (MQ=255)
                                                                                                                                                 cTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGa                                                                   >  1:439018/1‑87 (MQ=255)
                                                                                                                                                  ttCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCt                                                                      <  3:458111/83‑1 (MQ=255)
                                                                                                                                                  ttCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCt                                                                      >  4:458111/1‑83 (MQ=255)
                                                                                                                                                   tctcAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAgc   <  2:439894/149‑1 (MQ=255)
                                                                                                                                                   tctcAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAgc   <  1:594493/149‑1 (MQ=255)
                                                                                                                                                   tctcAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAgc   <  2:142049/149‑1 (MQ=255)
                                                                                                                                                    ctcAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAgcg  >  4:873272/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCG  >  NC_000913/3246414‑3246710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: