Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,332,823 T→C 9.8% intergenic (‑88/+39) yhbE ← / ← rpmA EamA family inner membrane putative transporter/50S ribosomal subunit protein L27

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,332,8230TC 9.8% 238.4 / 12.1 80intergenic (‑88/+39)yhbE/rpmAEamA family inner membrane putative transporter/50S ribosomal subunit protein L27
Reads supporting (aligned to +/- strand):  ref base T (33/39);  new base C (5/3);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

CCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCA  >  NC_000913/3332676‑3332963
                                                                                                                                                   |                                                                                                                                            
cccccAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACgtg                                                                                                                                             <  3:768394/149‑1 (MQ=255)
 ccccAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACgtgt                                                                                                                                            >  1:47938/1‑149 (MQ=255)
    cAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGc                                                                                                                                         <  1:8313/149‑1 (MQ=255)
      gCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCgg                                                                                                                                       <  2:351501/149‑1 (MQ=255)
       cAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGGTTAACGTCGCACCATGCGCATTTTTACCGGTTGTCGAATGTAAAAAGCCCCGCAACACGTTGCggg                                                                                                                                      <  3:254395/149‑1 (MQ=255)
       cAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCggg                                                                                                                                      <  4:897468/149‑1 (MQ=255)
             gCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACACGTTGCGGGGCtttt                                                                                                                                <  1:738471/149‑2 (MQ=255)
                gtggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATc                                                                                                                             <  3:305518/149‑1 (MQ=255)
                 tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATcc                                                                                                                            <  4:674755/149‑1 (MQ=255)
                    tgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGtt                                                                                                                         <  1:515296/149‑1 (MQ=255)
                         gcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCgg                                                                                                                    >  4:4334/1‑149 (MQ=255)
                           cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGa                                                                                                                  >  4:461253/1‑149 (MQ=255)
                           cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGa                                                                                                                  >  3:851169/1‑149 (MQ=255)
                           cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGa                                                                                                                  >  3:724357/1‑149 (MQ=255)
                            aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGAc                                                                                                                 >  1:176896/1‑149 (MQ=255)
                              aaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGAcgc                                                                                                               >  2:741680/1‑149 (MQ=255)
                              aaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGAcgc                                                                                                               <  2:47938/149‑1 (MQ=255)
                                     ccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAGAAc                                                                                                        <  3:902632/149‑1 (MQ=255)
                                     ccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAAc                                                                                                        <  4:885084/149‑1 (MQ=255)
                                      caatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACt                                                                                                       >  3:513766/1‑149 (MQ=255)
                                           cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTc                                                                                                  <  3:461253/149‑1 (MQ=255)
                                            ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCa                                                                                                 <  3:264331/149‑1 (MQ=255)
                                             tgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACTTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAg                                                                                                <  2:313195/149‑1 (MQ=255)
                                              gcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGc                                                                                               >  4:503696/1‑149 (MQ=255)
                                               cctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCt                                                                                              <  4:134076/149‑1 (MQ=255)
                                                 tgctgcTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTc                                                                                            <  4:509988/149‑1 (MQ=255)
                                                 tgctgcTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTc                                                                                            >  4:745615/1‑149 (MQ=255)
                                                 tgctgcTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTc                                                                                            >  3:677739/1‑149 (MQ=255)
                                                   ctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGa                                                                                          <  3:651139/149‑1 (MQ=255)
                                                     gctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTAt                                                                                                    <  1:458915/137‑1 (MQ=255)
                                                     gctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTAt                                                                                                    >  2:458915/1‑137 (MQ=255)
                                                     gctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATg                                                                                        >  3:143930/1‑149 (MQ=255)
                                                        tCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTCCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTg                                                                                     >  4:625463/1‑149 (MQ=255)
                                                         cATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGa                                                                                    >  2:717979/1‑149 (MQ=255)
                                                           tACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATa                                                                                  >  1:444418/1‑149 (MQ=255)
                                                               gTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAAtt                                                                              <  2:176896/149‑1 (MQ=255)
                                                                tATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAAttt                                                                             >  1:650471/1‑149 (MQ=255)
                                                                  ttCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTAc                                                                           >  3:536660/1‑149 (MQ=255)
                                                                     ccTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGt                                                                        >  2:893212/1‑149 (MQ=255)
                                                                       tGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCtt                                                                                             >  4:838024/1‑126 (MQ=255)
                                                                       tGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCtt                                                                                             <  3:838024/126‑1 (MQ=255)
                                                                         cAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGttttt                                                                    <  1:488656/149‑1 (MQ=255)
                                                                            aGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCgg                                                                 <  2:650471/149‑1 (MQ=255)
                                                                             gcgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGTTGCTGATAAATTTACGGTTTCTCggg                                                                <  4:790368/149‑1 (MQ=255)
                                                                             gcgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCggg                                                                <  4:513766/149‑1 (MQ=255)
                                                                               gcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGcc                                                              >  1:561650/1‑149 (MQ=255)
                                                                                cATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCt                                                             <  3:503451/149‑1 (MQ=255)
                                                                                 aTTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCtt                                                            >  4:333774/1‑149 (MQ=255)
                                                                                  tttGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCttt                                                           >  2:459056/1‑149 (MQ=255)
                                                                                  tttGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCttt                                                           >  3:546353/1‑149 (MQ=255)
                                                                                        gATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTc                                                     >  3:173818/1‑149 (MQ=255)
                                                                                         aTTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCg                                                    <  2:594924/149‑1 (MQ=255)
                                                                                           tAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGaa                                                  <  3:120377/149‑1 (MQ=255)
                                                                                                tCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAAttt                                               <  4:866480/147‑1 (MQ=255)
                                                                                                tCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAAttt                                               >  3:866480/1‑147 (MQ=255)
                                                                                                  gCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACt                                           <  3:4334/149‑1 (MQ=255)
                                                                                                      cATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTAc                                       <  2:250652/149‑1 (MQ=255)
                                                                                                              tttttACCGTTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCt                               <  3:745615/149‑1 (MQ=255)
                                                                                                               ttttACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCtt                              <  3:503696/149‑1 (MQ=255)
                                                                                                               ttttACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCtt                              <  4:189198/149‑1 (MQ=255)
                                                                                                                tttACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCttt                             <  1:459056/149‑1 (MQ=255)
                                                                                                                 ttACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTa                            <  3:699435/149‑1 (MQ=255)
                                                                                                                  tACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAg                           <  1:373163/149‑1 (MQ=255)
                                                                                                                     cGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCaa                        >  4:359456/1‑149 (MQ=255)
                                                                                                                      ggTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCaaa                       <  3:242246/149‑1 (MQ=255)
                                                                                                                       gTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAAc                      >  1:119078/1‑149 (MQ=255)
                                                                                                                        ttATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAAcg                     <  3:333774/149‑2 (MQ=255)
                                                                                                                            cGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAgt                 <  1:752943/149‑1 (MQ=255)
                                                                                                                              aaTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGtt                                                                       >  3:414064/1‑93 (MQ=255)
                                                                                                                              aaTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGtt                                                                       <  4:414064/93‑1 (MQ=255)
                                                                                                                              aaTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAgtgt               >  1:49006/1‑149 (MQ=255)
                                                                                                                               aTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGtgtg              <  3:176338/149‑1 (MQ=255)
                                                                                                                                  tAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTc           >  4:132526/1‑149 (MQ=255)
                                                                                                                                       atccccGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATGTACGGTTTTGCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGat      >  2:771915/3‑148 (MQ=255)
                                                                                                                                       aGCCCCGCAACACGTTGCGGGGCTTTCATCCGTTACAGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGAc      >  4:875368/1‑149 (MQ=255)
                                                                                                                                        gCCCCGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGAcc     >  4:361176/1‑149 (MQ=255)
                                                                                                                                         ccccGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCg    >  4:480225/1‑149 (MQ=255)
                                                                                                                                          cccGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACaga                   >  2:501976/1‑133 (MQ=255)
                                                                                                                                          cccGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACaga                   <  1:501976/133‑1 (MQ=255)
                                                                                                                                          cccGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGc   >  2:539322/1‑149 (MQ=255)
                                                                                                                                           ccGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCa  >  2:547212/1‑149 (MQ=255)
                                                                                                                                           ccGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCa  >  3:494804/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
CCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCA  >  NC_000913/3332676‑3332963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑