Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,474,031 C→G 6.1% K17N (AAG→AAC rpsG ← 30S ribosomal subunit protein S7

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,474,0310CG 6.1% 445.8 / 13.6 128K17N (AAG→AACrpsG30S ribosomal subunit protein S7
Reads supporting (aligned to +/- strand):  ref base C (58/61);  new base G (3/5);  total (62/66)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 128 total aligned reads displayed.

GCTACTTCGAATGCTTCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGGAGAACC  >  NC_000913/3473888‑3474176
                                                                                                                                               |                                                                                                                                                 
gCTACTTCGAATGCTTCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCgg                                                                                                                                              <  3:605641/149‑1 (MQ=255)
     ttCGAATGCTTCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCg                                                                                                                                         >  1:667448/1‑149 (MQ=255)
     ttCGAATGCTTCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCg                                                                                                                                         >  3:613855/1‑149 (MQ=255)
      tCGAATGCTTCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCgg                                                                                                                                        <  4:101542/149‑1 (MQ=255)
       cGAATGCTTCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAAGTTCGGATcagaa                                                                                                                                       <  1:931265/149‑5 (MQ=255)
       cGAATGCTTCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAAGTTCGGATcagaa                                                                                                                                       <  3:221844/149‑5 (MQ=255)
        gAATGCTTCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCa                                                                                                                                      >  4:784782/1‑149 (MQ=255)
        gAATGCTTCCAGTTCAGATTTACCAGAGCGCTGAGACAGGGTCTCCAGCGAGCTGTTTACGATAGATTCAGCAGTAGATATTTTGCCGTCTCCCATCAGGATATTTACAAATTTAGCCAGCAGCTCTGATCCGAAGTTCAGATCCGGCa                                                                                                                                      <  2:101277/149‑1 (MQ=255)
         aaTGCTTCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAg                                                                                                                                     >  3:437920/1‑149 (MQ=255)
         aaTGCTTCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAg                                                                                                                                     >  4:359084/1‑149 (MQ=255)
            gCTTCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAAt                                                                                                                                  <  1:275012/149‑1 (MQ=255)
             cTTCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAAtt                                                                                                                                 <  1:148126/149‑1 (MQ=255)
              ttCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAAttt                                                                                                                                >  1:264173/1‑149 (MQ=255)
              ttCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAAttt                                                                                                                                >  4:224868/1‑149 (MQ=255)
              ttCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAAttt                                                                                                                                >  2:914225/1‑149 (MQ=255)
              ttCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAAttt                                                                                                                                >  1:163095/1‑149 (MQ=255)
                 cAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTAc                                                                                                                             >  3:750533/1‑149 (MQ=255)
                 cAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTAc                                                                                                                             >  2:258861/1‑149 (MQ=255)
                  aGTTCAGATTTACCAGAGCGCTGACCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAGATTTAGCCAGCAGTTCTGATCCGAAATTCGGATCCGGCAGAATTTTACg                                                                                                                            >  3:578478/1‑149 (MQ=255)
                    ttCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCt                                                                                                                          >  3:463935/1‑149 (MQ=255)
                      cAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGa                                                                                                                        <  4:834957/149‑1 (MQ=255)
                        gATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGAcc                                                                                                                      >  1:89245/1‑149 (MQ=255)
                         aTTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCGGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCATCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCa                                                                                                                     >  3:79215/1‑149 (MQ=255)
                         aTTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGAt                                                                                                                                            <  3:734079/126‑1 (MQ=255)
                         aTTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCa                                                                                                                     >  1:85488/1‑149 (MQ=255)
                              ccAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGAc                                                                                                                <  1:698511/149‑1 (MQ=255)
                                agagCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTCCCATCTACCATCAGGATATTTACAATTTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCCATGAcgc                                                                                                              <  4:216977/149‑1 (MQ=255)
                                 gagCGCTGAGCCAGGGTCTCCAGCGCGCCGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGAcgcg                                                                                                             >  3:729906/1‑149 (MQ=255)
                                        gAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGt                                                                                                      <  4:505476/149‑1 (MQ=255)
                                          gCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACTATCAGGATATTGACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTgg                                                                                                    <  2:667448/149‑1 (MQ=255)
                                          gCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTgg                                                                                                    >  4:757935/1‑149 (MQ=255)
                                            cAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCa                                                                                                  >  3:557138/1‑149 (MQ=255)
                                             aGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCAt                                                                                                 >  2:712338/1‑149 (MQ=255)
                                                  ctcCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGaaa                                                                                            <  1:112545/149‑1 (MQ=255)
                                                   tcCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAAt                                                                                           >  2:488493/1‑149 (MQ=255)
                                                   tcCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAAt                                                                                           >  1:551184/1‑149 (MQ=255)
                                                   tcCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACAATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGCAAt                                                                                           >  4:743596/1‑149 (MQ=255)
                                                     cAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATAc                                                                                         >  1:518499/1‑149 (MQ=255)
                                                      aGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACt                                                                                        <  1:856948/149‑1 (MQ=255)
                                                        cgcgcTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTcc                                                                                      <  2:551184/149‑1 (MQ=255)
                                                           gcTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCATCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCgtt                                                                                   <  4:548575/149‑1 (MQ=255)
                                                           gcTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCgtt                                                                                   <  2:89245/149‑1 (MQ=255)
                                                             tGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCgttgt                                                                                 >  2:820576/1‑149 (MQ=255)
                                                               tataCGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTa                                                                               >  1:464400/1‑149 (MQ=255)
                                                                ataCGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTaa                                                                              <  3:784782/149‑1 (MQ=255)
                                                                    gATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGCTCCGAAGTTCGGATcaga                                                                                                                                        >  3:371976/1‑84 (MQ=255)
                                                                    gATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAAGtt                                                                                                                                                 >  2:558069/1‑78 (MQ=255)
                                                                    gATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAAGtt                                                                                                                                                 <  1:558069/78‑1 (MQ=255)
                                                                    gATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAAGTTCGGATcaga                                                                                                                                        <  4:371976/87‑4 (MQ=255)
                                                                         aTTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAg                                                                         >  2:80646/1‑145 (MQ=255)
                                                                         aTTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAg                                                                         <  1:80646/145‑1 (MQ=255)
                                                                         aTTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGAtt                                                                     <  4:231952/149‑1 (MQ=255)
                                                                         aTTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGAtt                                                                     <  4:24926/149‑1 (MQ=255)
                                                                            cagcagTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCAGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTc                                                                  <  3:875389/149‑1 (MQ=255)
                                                                             agcagTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCa                                                                          >  2:424399/1‑140 (MQ=255)
                                                                             agcagTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCa                                                                          <  1:424399/140‑1 (MQ=255)
                                                                                 gTAGATTTTTTACCATCTACCATCAGTATATTTACAAATTAAGCAATCAGTTCTAATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGCCGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCaaaa                                                             <  2:226687/149‑1 (MQ=255)
                                                                                 gTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCaaaa                                                             <  2:246300/149‑1 (MQ=255)
                                                                                  tAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTc                                                                           <  3:536904/134‑1 (MQ=255)
                                                                                  tAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTc                                                                           >  4:536904/1‑134 (MQ=255)
                                                                                   aGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAAct                                                           >  4:66970/1‑149 (MQ=255)
                                                                                    gATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGACATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAACctc                                                          <  2:128372/149‑1 (MQ=255)
                                                                                      ttttttACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTTATTCAGGATTGTCCAAAACTCTa                                                        >  4:432632/1‑149 (MQ=255)
                                                                                         tttACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGa                                                     <  4:701567/149‑1 (MQ=255)
                                                                                              catctaccatcAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGagttta                                                <  4:79215/149‑1 (MQ=255)
                                                                                              catctaccatcAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGagtt                                                  <  1:654406/147‑1 (MQ=255)
                                                                                              catctaccatcAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTACAAAACTCTACGagttta                                                >  3:640126/1‑149 (MQ=255)
                                                                                                tctaccatcAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGagtttagt                                              >  1:106032/1‑149 (MQ=255)
                                                                                                   accatcAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTg                                           >  3:219933/1‑149 (MQ=255)
                                                                                                    ccatcAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGa                                          >  4:383956/1‑149 (MQ=255)
                                                                                                      atcAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACa                                        >  4:528171/1‑149 (MQ=255)
                                                                                                       tcAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGa                                                                                                                        >  3:320744/1‑68 (MQ=255)
                                                                                                       tcAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGa                                                                                                                        <  4:320744/68‑1 (MQ=255)
                                                                                                       tcAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTc                                                                                       <  3:382278/101‑1 (MQ=255)
                                                                                                       tcAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTc                                                                                       >  4:382278/1‑101 (MQ=255)
                                                                                                          ggATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTa                                    >  1:139600/1‑149 (MQ=255)
                                                                                                                  aCAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTaaaa                            <  1:914225/149‑1 (MQ=255)
                                                                                                                  aCAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGAGCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTaaaa                            >  4:874539/1‑149 (MQ=255)
                                                                                                                       tttAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGttt                       <  3:770235/149‑1 (MQ=255)
                                                                                                                       tttAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGttt                       <  4:862828/149‑1 (MQ=255)
                                                                                                                       tttAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGttt                       >  1:118362/1‑149 (MQ=255)
                                                                                                                       tttAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGttt                       <  4:729906/149‑1 (MQ=255)
                                                                                                                       tttAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGttt                       <  2:839542/149‑1 (MQ=255)
                                                                                                                         tAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTgg                     <  3:517984/149‑1 (MQ=255)
                                                                                                                         tAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTgg                     <  2:461908/149‑1 (MQ=255)
                                                                                                                          aGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGc                    >  1:45583/1‑149 (MQ=255)
                                                                                                                            ccAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCct                  >  1:223268/1‑149 (MQ=255)
                                                                                                                             cagcagTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCctt                 >  1:409511/1‑149 (MQ=255)
                                                                                                                              agcagTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCctta                <  1:12318/149‑1 (MQ=255)
                                                                                                                               gcagTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCcttac               <  3:823713/149‑1 (MQ=255)
                                                                                                                                     cTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAg                                                                         >  1:826317/1‑85 (MQ=255)
                                                                                                                                     cTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAg                                                                         <  2:826317/85‑1 (MQ=255)
                                                                                                                                     cTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACg         <  2:518499/149‑1 (MQ=255)
                                                                                                                                      tGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACgg        >  2:461357/1‑149 (MQ=255)
                                                                                                                                        aTCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGgag      >  3:901079/1‑149 (MQ=255)
                                                                                                                                         tCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCctt                 >  4:164470/1‑137 (MQ=255)
                                                                                                                                         tCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCctt                 <  3:164470/137‑1 (MQ=255)
                                                                                                                                          ccGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGGAGaa    <  3:285531/149‑1 (MQ=255)
                                                                                                                                            gAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGGAGAAcc  >  1:259559/1‑149 (MQ=255)
                                                                                                                                            gAACTTCAGATCCGGCAGAATTTTACGCTGACCAATGACGCTACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTACTAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAGACGTTTGGCCTTACTTAACGGAGAAcc  <  2:671476/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GCTACTTCGAATGCTTCCAGTTCAGATTTACCAGAGCGCTGAGCCAGGGTCTCCAGCGCGCTGTATACGATAGATTCAGCAGTAGATTTTTTACCATCTACCATCAGGATATTTACAAATTTAGCCAGCAGTTCTGATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGGAGAACC  >  NC_000913/3473888‑3474176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: