Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,480,662 T→A 7.7% H167L (CAC→CTC)  kefG ← potassium‑efflux system ancillary protein for KefB, glutathione‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,480,6620TA 7.7% 319.9 / 13.2 91H167L (CAC→CTC) kefGpotassium‑efflux system ancillary protein for KefB, glutathione‑regulated
Reads supporting (aligned to +/- strand):  ref base T (38/46);  new base A (3/4);  total (41/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GCGCCAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAG  >  NC_000913/3480516‑3480809
                                                                                                                                                  |                                                                                                                                                   
gcgcCAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTgg                                                                                                                                                   >  1:840235/1‑149 (MQ=255)
  gcCAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCt                                                                                                                                                 >  4:688160/1‑149 (MQ=255)
  gcCAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCt                                                                                                                                                 >  1:52583/1‑149 (MQ=255)
    cAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCg                                                                                                                                               >  3:842525/1‑149 (MQ=255)
      aTACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGcc                                                                                                                                             <  3:796214/149‑1 (MQ=255)
      aTACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGcc                                                                                                                                             >  3:107826/1‑149 (MQ=255)
       tACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCa                                                                                                                                            >  2:527836/1‑149 (MQ=255)
         cccAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCCAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAga                                                                                                                                          <  2:432854/149‑2 (MQ=255)
          ccAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCt                                                                                                                                         <  2:865159/149‑1 (MQ=255)
          ccAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCt                                                                                                                                         >  3:169552/1‑149 (MQ=255)
           cAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTc                                                                                                                                        <  1:917247/149‑1 (MQ=255)
            aGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAgagcc                                                                                                                                       <  3:74032/149‑5 (MQ=255)
               cgcgATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCtgt                                                                                                                                    <  1:87090/149‑1 (MQ=255)
                  gATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTgcg                                                                                                                                 >  1:805181/1‑149 (MQ=255)
                   aTGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTgcgc                                                                                                                                <  4:5397/149‑1 (MQ=255)
                         gCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGcc                                                                                                                          >  1:405399/1‑149 (MQ=255)
                              aCCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGtctc                                                                                                                     <  3:421532/149‑1 (MQ=255)
                                  cAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGccc                                                                                                                 <  3:732244/149‑1 (MQ=255)
                                       aCCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTaa                                                                                                            >  3:112250/1‑149 (MQ=255)
                                       aCCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAgag                                                                                                                                         <  1:645824/120‑3 (MQ=255)
                                       aCCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAgag                                                                                                                                         >  2:645824/1‑118 (MQ=255)
                                           ccgcGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAaataa                                                                                                        <  4:803349/149‑1 (MQ=255)
                                               gAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgat                                                                                                    <  3:514184/149‑1 (MQ=255)
                                                aaGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgatg                                                                                                   >  1:281203/1‑149 (MQ=255)
                                                 agagAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATgg                                                                                                  <  2:728133/149‑1 (MQ=255)
                                                 agagAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATgg                                                                                                  >  3:783753/1‑149 (MQ=255)
                                                  gagaAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATggg                                                                                                 >  3:321669/1‑149 (MQ=255)
                                                   agaAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGa                                                                                                <  2:172531/149‑1 (MQ=255)
                                                     aaaCAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACt                                                                                              >  1:502635/1‑149 (MQ=255)
                                                       aCAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGcc                                                                                                                          >  2:581468/1‑119 (MQ=255)
                                                       aCAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGcc                                                                                                                          <  1:581468/119‑1 (MQ=255)
                                                        cAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTaa                                                                                           <  3:220225/149‑1 (MQ=255)
                                                        cAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTaa                                                                                           <  2:281203/149‑1 (MQ=255)
                                                         aGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAAc                                                                                          <  2:142922/149‑1 (MQ=255)
                                                         aGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAAc                                                                                          <  3:688160/149‑1 (MQ=255)
                                                            aCTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                       >  4:161033/1‑149 (MQ=255)
                                                             cTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAAt                                                                                      <  1:527836/149‑1 (MQ=255)
                                                              tCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATg                                                                                     <  1:903620/149‑1 (MQ=255)
                                                               ccTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCCTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGc                                                                                    <  2:823806/149‑1 (MQ=255)
                                                                cTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCa                                                                                   >  4:146072/1‑149 (MQ=255)
                                                                cTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCa                                                                                   >  3:817687/1‑149 (MQ=255)
                                                                 tGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCAt                                                                                  >  2:456613/1‑149 (MQ=255)
                                                                       tAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGca                                                                            >  2:558872/1‑149 (MQ=255)
                                                                       tAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGca                                                                            >  4:602906/1‑149 (MQ=255)
                                                                          aaaaTCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGTGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACAt                                                                         >  4:430759/1‑149 (MQ=255)
                                                                          aaaaTCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACAt                                                                         >  3:885911/1‑149 (MQ=255)
                                                                            aaTCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGc                                                                       >  3:78941/1‑149 (MQ=255)
                                                                            aaTCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGc                                                                       <  4:107826/149‑1 (MQ=255)
                                                                                  aaCCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCccgccg                                                                 >  2:100467/1‑149 (MQ=255)
                                                                                    ccTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTGTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCgccgcc                                                               >  3:568374/1‑149 (MQ=255)
                                                                                      ttCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATgg                                                                                                  <  4:207564/112‑1 (MQ=255)
                                                                                      ttCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATgg                                                                                                  >  3:207564/1‑112 (MQ=255)
                                                                                      ttCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAg                                                             >  1:821318/1‑149 (MQ=255)
                                                                                        ccATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGtt                                                           <  4:842525/149‑1 (MQ=255)
                                                                                         cATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAAc                                                                                          <  1:221291/117‑1 (MQ=255)
                                                                                         cATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAAc                                                                                          >  2:221291/1‑117 (MQ=255)
                                                                                          aTCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCa                                                         >  3:729867/1‑149 (MQ=255)
                                                                                            cAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaaa                                                       <  3:602906/149‑1 (MQ=255)
                                                                                            cAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaaa                                                       <  1:558872/149‑1 (MQ=255)
                                                                                            cAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaaa                                                       <  4:112250/149‑1 (MQ=255)
                                                                                               cGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAggg                                                    <  4:532542/149‑1 (MQ=255)
                                                                                                   ctcctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGgcgc                                                <  3:195249/149‑1 (MQ=255)
                                                                                                    tcctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCgca                                               >  4:697739/1‑149 (MQ=255)
                                                                                                     cctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCgcag                                              <  3:161033/149‑1 (MQ=255)
                                                                                                      ctGGAGACAGAGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCgcagc                                             <  1:348142/149‑1 (MQ=255)
                                                                                                       tGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCgcagca                                            <  4:724130/149‑1 (MQ=255)
                                                                                                           gaCAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATc                                        <  4:658479/149‑1 (MQ=255)
                                                                                                                    tttGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCccgcc                                                                  <  3:821994/114‑1 (MQ=255)
                                                                                                                    ttagccagccaGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCAGCACATGCccgcc                                                                  >  4:821994/4‑114 (MQ=255)
                                                                                                                      tGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGt                             <  2:821318/149‑1 (MQ=255)
                                                                                                                      tGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGt                             <  4:746874/149‑1 (MQ=255)
                                                                                                                          agccagTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCg                         <  1:456613/149‑1 (MQ=255)
                                                                                                                           gccagTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGa                        >  2:665825/1‑149 (MQ=255)
                                                                                                                           gccagTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGa                        <  4:321669/149‑1 (MQ=255)
                                                                                                                               gTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCa                    <  2:242500/149‑1 (MQ=255)
                                                                                                                                tCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAg                   >  3:725063/1‑149 (MQ=255)
                                                                                                                                 cACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAgc                  <  3:44097/149‑1 (MQ=255)
                                                                                                                                   ccGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAgcgc                <  3:851695/149‑1 (MQ=255)
                                                                                                                                      tAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTc             <  2:526099/149‑1 (MQ=255)
                                                                                                                                       aGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCa            >  3:508846/1‑149 (MQ=255)
                                                                                                                                         gagctGGCGAGCCACGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATa          >  4:491950/4‑149 (MQ=255)
                                                                                                                                          ctctGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATat         >  1:669159/1‑148 (MQ=255)
                                                                                                                                          ctctGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATaa         <  3:317459/149‑1 (MQ=255)
                                                                                                                                           tctGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTCAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAAc        >  3:631357/1‑149 (MQ=255)
                                                                                                                                           gctGGCGAGCCACGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaaa                                                       >  1:684771/2‑102 (MQ=255)
                                                                                                                                           gctGGCGAGCCACGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaaa                                                       <  2:684771/101‑1 (MQ=255)
                                                                                                                                               gCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTa    <  4:351073/149‑1 (MQ=255)
                                                                                                                                                cGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTaa   <  2:840235/149‑1 (MQ=255)
                                                                                                                                                cGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTaa   <  3:430759/149‑1 (MQ=255)
                                                                                                                                                cGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTaa   <  1:204904/149‑1 (MQ=255)
                                                                                                                                                 gTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGCCTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAg  >  3:562346/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCGCCAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAG  >  NC_000913/3480516‑3480809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑