Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,480,666 T→A 7.9% S166C (AGC→TGC) ‡ kefG ← potassium‑efflux system ancillary protein for KefB, glutathione‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,480,6660TA 7.9% 310.0 / 12.2 89S166C (AGC→TGC) ‡kefGpotassium‑efflux system ancillary protein for KefB, glutathione‑regulated
Reads supporting (aligned to +/- strand):  ref base T (35/47);  new base A (3/4);  total (38/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTT  >  NC_000913/3480518‑3480814
                                                                                                                                                    |                                                                                                                                                    
gcCAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCt                                                                                                                                                      >  4:688160/1‑149 (MQ=255)
gcCAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCt                                                                                                                                                      >  1:52583/1‑149 (MQ=255)
  cAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCg                                                                                                                                                    >  3:842525/1‑149 (MQ=255)
    aTACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGcc                                                                                                                                                  >  3:107826/1‑149 (MQ=255)
    aTACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGcc                                                                                                                                                  <  3:796214/149‑1 (MQ=255)
     tACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCa                                                                                                                                                 >  2:527836/1‑149 (MQ=255)
       cccAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCCAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAga                                                                                                                                               <  2:432854/149‑2 (MQ=255)
        ccAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCt                                                                                                                                              <  2:865159/149‑1 (MQ=255)
        ccAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCt                                                                                                                                              >  3:169552/1‑149 (MQ=255)
         cAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTc                                                                                                                                             <  1:917247/149‑1 (MQ=255)
          aGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAgagcc                                                                                                                                            <  3:74032/149‑5 (MQ=255)
             cgcgATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCtgt                                                                                                                                         <  1:87090/149‑1 (MQ=255)
                gATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTgcg                                                                                                                                      >  1:805181/1‑149 (MQ=255)
                 aTGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTgcgc                                                                                                                                     <  4:5397/149‑1 (MQ=255)
                       gCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGcc                                                                                                                               >  1:405399/1‑149 (MQ=255)
                            aCCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGtctc                                                                                                                          <  3:421532/149‑1 (MQ=255)
                                cAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGccc                                                                                                                      <  3:732244/149‑1 (MQ=255)
                                     aCCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTaa                                                                                                                 >  3:112250/1‑149 (MQ=255)
                                     aCCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAgag                                                                                                                                              <  1:645824/120‑3 (MQ=255)
                                     aCCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAgag                                                                                                                                              >  2:645824/1‑118 (MQ=255)
                                         ccgcGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAaataa                                                                                                             <  4:803349/149‑1 (MQ=255)
                                             gAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgat                                                                                                         <  3:514184/149‑1 (MQ=255)
                                              aaGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgatg                                                                                                        >  1:281203/1‑149 (MQ=255)
                                               agagAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATgg                                                                                                       >  3:783753/1‑149 (MQ=255)
                                               agagAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATgg                                                                                                       <  2:728133/149‑1 (MQ=255)
                                                gagaAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATggg                                                                                                      >  3:321669/1‑149 (MQ=255)
                                                 agaAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGa                                                                                                     <  2:172531/149‑1 (MQ=255)
                                                   aaaCAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACt                                                                                                   >  1:502635/1‑149 (MQ=255)
                                                     aCAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGcc                                                                                                                               >  2:581468/1‑119 (MQ=255)
                                                     aCAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGcc                                                                                                                               <  1:581468/119‑1 (MQ=255)
                                                      cAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTaa                                                                                                <  2:281203/149‑1 (MQ=255)
                                                      cAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTaa                                                                                                <  3:220225/149‑1 (MQ=255)
                                                       aGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAAc                                                                                               <  2:142922/149‑1 (MQ=255)
                                                       aGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAAc                                                                                               <  3:688160/149‑1 (MQ=255)
                                                          aCTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                            >  4:161033/1‑149 (MQ=255)
                                                           cTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAAt                                                                                           <  1:527836/149‑1 (MQ=255)
                                                            tCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATg                                                                                          <  1:903620/149‑1 (MQ=255)
                                                             ccTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCCTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGc                                                                                         <  2:823806/149‑1 (MQ=255)
                                                              cTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCa                                                                                        >  4:146072/1‑149 (MQ=255)
                                                              cTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCa                                                                                        >  3:817687/1‑149 (MQ=255)
                                                               tGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCAt                                                                                       >  2:456613/1‑149 (MQ=255)
                                                                     tAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGca                                                                                 >  2:558872/1‑149 (MQ=255)
                                                                     tAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGca                                                                                 >  4:602906/1‑149 (MQ=255)
                                                                        aaaaTCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGTGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACAt                                                                              >  4:430759/1‑149 (MQ=255)
                                                                        aaaaTCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACAt                                                                              >  3:885911/1‑149 (MQ=255)
                                                                          aaTCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGc                                                                            <  4:107826/149‑1 (MQ=255)
                                                                          aaTCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGc                                                                            >  3:78941/1‑149 (MQ=255)
                                                                                aaCCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCccgccg                                                                      >  2:100467/1‑149 (MQ=255)
                                                                                  ccTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTGTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCgccgcc                                                                    >  3:568374/1‑149 (MQ=255)
                                                                                    ttCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATgg                                                                                                       >  3:207564/1‑112 (MQ=255)
                                                                                    ttCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATgg                                                                                                       <  4:207564/112‑1 (MQ=255)
                                                                                    ttCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAg                                                                  >  1:821318/1‑149 (MQ=255)
                                                                                      ccATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGtt                                                                <  4:842525/149‑1 (MQ=255)
                                                                                       cATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAAc                                                                                               <  1:221291/117‑1 (MQ=255)
                                                                                       cATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAAc                                                                                               >  2:221291/1‑117 (MQ=255)
                                                                                        aTCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCa                                                              >  3:729867/1‑149 (MQ=255)
                                                                                          cAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaaa                                                            <  3:602906/149‑1 (MQ=255)
                                                                                          cAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaaa                                                            <  1:558872/149‑1 (MQ=255)
                                                                                          cAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaaa                                                            <  4:112250/149‑1 (MQ=255)
                                                                                             cGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAggg                                                         <  4:532542/149‑1 (MQ=255)
                                                                                                 ctcctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGgcgc                                                     <  3:195249/149‑1 (MQ=255)
                                                                                                  tcctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCgca                                                    >  4:697739/1‑149 (MQ=255)
                                                                                                   cctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCgcag                                                   <  3:161033/149‑1 (MQ=255)
                                                                                                    ctGGAGACAGAGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCgcagc                                                  <  1:348142/149‑1 (MQ=255)
                                                                                                     tGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCgcagca                                                 <  4:724130/149‑1 (MQ=255)
                                                                                                         gaCAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATc                                             <  4:658479/149‑1 (MQ=255)
                                                                                                                  tttGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCccgcc                                                                       <  3:821994/114‑1 (MQ=255)
                                                                                                                  ttagccagccaGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCAGCACATGCccgcc                                                                       >  4:821994/4‑114 (MQ=255)
                                                                                                                    tGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGt                                  <  4:746874/149‑1 (MQ=255)
                                                                                                                    tGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGt                                  <  2:821318/149‑1 (MQ=255)
                                                                                                                        agccagTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCg                              <  1:456613/149‑1 (MQ=255)
                                                                                                                         gccagTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGa                             <  4:321669/149‑1 (MQ=255)
                                                                                                                         gccagTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGa                             >  2:665825/1‑149 (MQ=255)
                                                                                                                             gTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCa                         <  2:242500/149‑1 (MQ=255)
                                                                                                                              tCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAg                        >  3:725063/1‑149 (MQ=255)
                                                                                                                               cACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAgc                       <  3:44097/149‑1 (MQ=255)
                                                                                                                                 ccGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAgcgc                     <  3:851695/149‑1 (MQ=255)
                                                                                                                                    tAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTc                  <  2:526099/149‑1 (MQ=255)
                                                                                                                                     aGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCa                 >  3:508846/1‑149 (MQ=255)
                                                                                                                                       gagctGGCGAGCCACGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATa               >  4:491950/4‑149 (MQ=255)
                                                                                                                                        ctctGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATat              >  1:669159/1‑148 (MQ=255)
                                                                                                                                        ctctGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATaa              <  3:317459/149‑1 (MQ=255)
                                                                                                                                         tctGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTCAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAAc             >  3:631357/1‑149 (MQ=255)
                                                                                                                                         gctGGCGAGCCACGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaaa                                                            <  2:684771/101‑1 (MQ=255)
                                                                                                                                         gctGGCGAGCCACGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaaa                                                            >  1:684771/2‑102 (MQ=255)
                                                                                                                                             gCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTa         <  4:351073/149‑1 (MQ=255)
                                                                                                                                              cGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTaa        <  1:204904/149‑1 (MQ=255)
                                                                                                                                              cGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTaa        <  2:840235/149‑1 (MQ=255)
                                                                                                                                              cGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTaa        <  3:430759/149‑1 (MQ=255)
                                                                                                                                               gTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGCCTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAg       >  3:562346/1‑149 (MQ=255)
                                                                                                                                                  gCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCAc    <  4:784862/149‑1 (MQ=255)
                                                                                                                                                    tCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCggg                                   >  4:117174/1‑116 (MQ=255)
                                                                                                                                                    tCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCggg                                   <  3:117174/116‑1 (MQ=255)
                                                                                                                                                    tCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACtt  <  4:232219/149‑1 (MQ=255)
                                                                                                                                                    tCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTCAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACtt  >  3:79738/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTT  >  NC_000913/3480518‑3480814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: