Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,842,796 G→T 20.4% L332M (CTG→ATG)  adeQ ← adenine permease, high affinity; adenine:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,842,7960GT20.4% 277.6 / 56.2 103L332M (CTG→ATG) adeQadenine permease, high affinity; adenine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base G (41/41);  new base T (10/11);  total (51/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01

Only 100 of 103 total aligned reads displayed.

ATCCCAGTTAACGCGCGCCAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGAATTTACCGTTACCATCTAT  >  NC_000913/3842650‑3842943
                                                                                                                                                  |                                                                                                                                                   
atcCCAGTTAACGCGCGCCAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGcc                                                                                                                                                   <  3:861022/149‑1 (MQ=255)
  cccAGTTAACGCGCGCCAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGcccc                                                                                                                                                 >  4:620641/1‑149 (MQ=255)
   ccAGTTAACGCGCGCCAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCg                                                                                                                                                >  4:531027/1‑149 (MQ=255)
   ccAGTTAACGCGCGCCAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCg                                                                                                                                                >  3:373962/1‑149 (MQ=255)
    cAGTTAACGCGCGCCAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGt                                                                                                                                               >  1:827673/1‑149 (MQ=255)
       ttAACGCGCGCCAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCg                                                                                                                                            <  1:821262/149‑1 (MQ=255)
         aaCGCGCGCCAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGc                                                                                                                                          <  4:767140/149‑1 (MQ=255)
            gcgcgcCAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCAc                                                                                                                                       <  2:475712/149‑1 (MQ=255)
              gcgcCAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCg                                                                                                                                     >  2:160947/1‑149 (MQ=255)
                 ccAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACt                                                                                                                                  <  1:542869/149‑1 (MQ=255)
                 ccAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCGCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACt                                                                                                                                  <  4:314904/149‑1 (MQ=255)
                  cAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTg                                                                                                                                 >  3:275867/1‑149 (MQ=255)
                  cAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTg                                                                                                                                 >  4:615218/1‑149 (MQ=255)
                   aGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGc                                                                                                                                <  3:259642/149‑1 (MQ=255)
                      cTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCcac                                                                                                                             >  1:819423/1‑149 (MQ=255)
                       tCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGGACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCcaca                                                                                                                            >  4:566208/1‑149 (MQ=255)
                       tCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCcaca                                                                                                                            >  1:632340/1‑149 (MQ=255)
                        cGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTTAGCCCCGTGCGGCCACCGACTGCCacac                                                                                                                           <  1:886521/149‑1 (MQ=255)
                             tcatcaGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGc                                                                                                                                <  4:231097/139‑1 (MQ=255)
                             tcatcaGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGc                                                                                                                                >  3:231097/1‑139 (MQ=255)
                             tcatcaGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGaa                                                                                                                      <  4:541991/149‑1 (MQ=255)
                             tcataaGCACGACAACAAAGATTAACGCCCCGGCGGTTGTGTAAGGAGGAACGATCGCGACCAGCGGTGAGAAGAACATACCCACCAGGAAAATAACGCCAACCACACCCGCCGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGac                                                                                                                      <  2:905906/149‑2 (MQ=255)
                               atcaGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTGAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGt                                                                                                                    >  3:332547/1‑149 (MQ=255)
                               atcaGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGt                                                                                                                    >  2:307930/1‑149 (MQ=255)
                               atcaGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGt                                                                                                                    >  2:283370/1‑149 (MQ=255)
                               atcaGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGt                                                                                                                    >  2:129287/1‑149 (MQ=255)
                               atcaGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGcc                                                                                                                                                   >  3:49359/1‑118 (MQ=255)
                               atcaGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGcc                                                                                                                                                   <  4:49359/118‑1 (MQ=255)
                               atcaGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCTACCGACTGCCACACCAGAAga                                                                                                                    >  1:905906/1‑148 (MQ=255)
                                tcaGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTa                                                                                                                   <  1:58188/149‑1 (MQ=255)
                                 caGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTAc                                                                                                                  <  3:290079/149‑1 (MQ=255)
                                   gCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACtt                                                                                                                <  2:309031/149‑1 (MQ=255)
                                    cACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACttt                                                                                                               >  3:804110/1‑149 (MQ=255)
                                       gCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCaa                                                                                                            <  1:253200/149‑1 (MQ=255)
                                            caaAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAg                                                                                                       <  1:739447/149‑1 (MQ=255)
                                            caaAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAg                                                                                                       <  2:844451/149‑1 (MQ=255)
                                            caaAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAg                                                                                                       <  3:767474/149‑1 (MQ=255)
                                              aaGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGc                                                                                                     <  3:440256/149‑1 (MQ=255)
                                               aGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCa                                                                                                    <  2:317597/149‑1 (MQ=255)
                                               aGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCACAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCa                                                                                                    <  1:224721/149‑1 (MQ=255)
                                                  ttAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTa                                                                                                 >  4:849963/1‑149 (MQ=255)
                                                   tAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTaa                                                                                                <  4:399491/149‑1 (MQ=255)
                                                       gCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGa                                                                                            >  1:679655/1‑149 (MQ=255)
                                                        cTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGAc                                                                                           <  2:666375/149‑1 (MQ=255)
                                                         tCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACg                                                                                          >  2:905926/1‑149 (MQ=255)
                                                          ccGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGa                                                                                         <  1:842620/149‑1 (MQ=255)
                                                             gcggTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGt                                                                                      <  1:790706/149‑1 (MQ=255)
                                                             gcggTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGt                                                                                      <  4:246400/149‑1 (MQ=255)
                                                             gcggTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGt                                                                                      <  3:532642/149‑1 (MQ=255)
                                                               ggTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGc                                                                                    <  1:307930/149‑1 (MQ=255)
                                                                gTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGcc                                                                                   <  1:244464/149‑1 (MQ=255)
                                                                      tAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATaaa                                                                             >  4:635754/1‑149 (MQ=255)
                                                                      tAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGc                                                                                    <  2:503494/142‑1 (MQ=255)
                                                                      tAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGc                                                                                    >  1:503494/1‑142 (MQ=255)
                                                                       aaGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAAc                                                                            <  3:527395/149‑1 (MQ=255)
                                                                       aaGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAAc                                                                            <  2:548392/149‑1 (MQ=255)
                                                                       aaGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCACCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAAc                                                                            <  1:43181/149‑1 (MQ=255)
                                                                           aggaACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCAc                                                                        >  3:810041/1‑149 (MQ=255)
                                                                            ggaACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCAcc                                                                       >  4:890093/1‑149 (MQ=255)
                                                                               aCTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGc                                                                    >  2:223956/1‑149 (MQ=255)
                                                                                 tATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCa                                                                  >  1:368146/1‑149 (MQ=255)
                                                                                   tCGCCACCAGCGGTGAGAAGAACATACCCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCAcc                                                                >  3:573504/1‑149 (MQ=255)
                                                                                    cGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCg                                                               >  2:602231/1‑149 (MQ=255)
                                                                                           aGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGAc                                                        >  2:552866/1‑149 (MQ=255)
                                                                                              ggTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCt                                                     <  2:368146/149‑1 (MQ=255)
                                                                                                 gagaAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATc                                                  <  1:234298/149‑1 (MQ=255)
                                                                                                   gaagaaCATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCaa                                                >  1:918030/1‑149 (MQ=255)
                                                                                                   gaagaaCATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCaa                                                <  2:819423/149‑1 (MQ=255)
                                                                                                    aagaaCATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTCACGCTATCAAc                                               <  3:597392/149‑1 (MQ=255)
                                                                                                      gaaCATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAAtata                                                                                                        >  3:492515/1‑90 (MQ=255)
                                                                                                      gaaCATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAAtata                                                                                                        <  4:492515/90‑1 (MQ=255)
                                                                                                      gaaCATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACAt                                             >  1:868937/1‑149 (MQ=255)
                                                                                                      gaaCATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACAt                                             >  3:678475/1‑149 (MQ=255)
                                                                                                       aaCATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATa                                            <  4:332547/149‑1 (MQ=255)
                                                                                                        aCATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATAc                                           >  2:620973/1‑149 (MQ=255)
                                                                                                          aTAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGa                                                                                         >  1:181903/1‑101 (MQ=255)
                                                                                                          aTAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGa                                                                                         <  2:181903/101‑1 (MQ=255)
                                                                                                             aCCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAgcgc                                      >  1:661209/1‑149 (MQ=255)
                                                                                                              ccAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGcc                                     >  1:703511/1‑149 (MQ=255)
                                                                                                               cAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCt                                    >  1:417374/1‑149 (MQ=255)
                                                                                                                aaCAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCtt                                   >  1:127737/1‑149 (MQ=255)
                                                                                                                    ggAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTc                               <  3:917365/149‑1 (MQ=255)
                                                                                                                      aaCATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCat                             >  4:898489/1‑149 (MQ=255)
                                                                                                                        cATAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCatat                           <  1:129287/149‑1 (MQ=255)
                                                                                                                         aTAACGCCAACCACAACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATAtt                          >  3:393406/1‑149 (MQ=255)
                                                                                                                          tAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGATGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTg                         >  2:114930/1‑149 (MQ=255)
                                                                                                                           aaCGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTgg                        <  1:160947/149‑1 (MQ=255)
                                                                                                                              gCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGa                     >  3:24510/1‑149 (MQ=255)
                                                                                                                                    cacaACCGCAGTCATCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGAATTTAc               >  4:389709/1‑149 (MQ=255)
                                                                                                                                    cacaACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGAATTTAc               >  3:611593/1‑149 (MQ=255)
                                                                                                                                    cacaACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGAATTTAc               <  2:703511/149‑1 (MQ=255)
                                                                                                                                    cacaACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGAATTTAc               <  4:678475/149‑1 (MQ=255)
                                                                                                                                     acaACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGAATTTAcc              >  3:427040/1‑149 (MQ=255)
                                                                                                                                         ccGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGAATTTACCGTTa          >  2:928107/1‑149 (MQ=255)
                                                                                                                                         ccGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGAATTTACCGTTa          >  2:617872/1‑149 (MQ=255)
                                                                                                                                         ccGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGAATTTACCGTTa          <  4:24510/149‑1 (MQ=255)
                                                                                                                                          cGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGAATTTACCGTTAc         <  4:373962/149‑1 (MQ=255)
                                                                                                                                                cAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGAATTTACCGTTACCATCTa   <  2:510322/149‑1 (MQ=255)
                                                                                                                                                 aGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGAATTTACCGTTACCATCTAt  <  2:417374/149‑1 (MQ=255)
                                                                                                                                                 aGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGAATTTACCGTTACCATCTAt  <  3:890093/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATCCCAGTTAACGCGCGCCAGGCTCGAAGTCATCAGCACGCCAACAAAGATTAACGCTCCGGCGGTTGCGTAAGGAGGAACTATCGCCACCAGCGGTGAGAAGAACATAACCAACAGGAACATAACGCCAACCACAACCGCAGTCAGCCCCGTGCGGCCACCGACTGCCACACCAGAAGTACTTTCAATATAGGCAGTAACAGACGAGGTGCCGATAAACGCACCCGCCACCGAACTGACGCTATCAACATACAGCGCCTTATTCATATTGGGGAATTTACCGTTACCATCTAT  >  NC_000913/3842650‑3842943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: