Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,980,726 G→A 6.9% intergenic (+30/‑161) wecG → / → yifK UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase/putative APC family amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,980,7260GA 6.9% 323.4 / 10.9 101intergenic (+30/‑161)wecG/yifKUDP‑N‑acetyl‑D‑mannosaminuronic acid transferase/putative APC family amino acid transporter
Reads supporting (aligned to +/- strand):  ref base G (46/48);  new base A (4/3);  total (50/51)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01

Only 100 of 101 total aligned reads displayed.

AATCTGGCAAACGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAA  >  NC_000913/3980579‑3980870
                                                                                                                                                   |                                                                                                                                                
aaTCTGGCAAACGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGt                                                                                                                                                 <  4:289059/149‑1 (MQ=255)
  tCTGGCAAACGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCa                                                                                                                                               >  4:899516/1‑149 (MQ=255)
   cTGGCAAACGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCAc                                                                                                                                              <  3:899920/149‑1 (MQ=255)
        aaaCGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGt                                                                                                                                         >  1:665938/1‑149 (MQ=255)
         aaCGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGACCACTCCGTc                                                                                                                                        <  4:620946/149‑1 (MQ=255)
             cTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCt                                                                                                                                    <  2:71410/149‑1 (MQ=255)
                gggCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTc                                                                                                                                 <  3:889079/149‑1 (MQ=255)
                      gAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCttt                                                                                                                           >  1:503190/1‑149 (MQ=255)
                       aGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCtttt                                                                                                                          <  4:52774/149‑1 (MQ=255)
                        gTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCttttt                                                                                                                         >  1:859080/1‑149 (MQ=255)
                        gTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCttttt                                                                                                                         >  2:69773/1‑149 (MQ=255)
                        gTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCttttt                                                                                                                         >  1:775959/1‑149 (MQ=255)
                          ggCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTtta                                                                                                                       <  2:414833/149‑1 (MQ=255)
                           gCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGACCACTCCGTCGct                                                                                                                                     >  2:424416/1‑133 (MQ=255)
                           gCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGACCACTCCGTCGct                                                                                                                                     <  1:424416/134‑2 (MQ=255)
                                  cGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACaa                                                                                                               >  3:910105/1‑149 (MQ=255)
                                   gCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTacac                                                                                                                 >  2:822879/1‑146 (MQ=255)
                                   gCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTacac                                                                                                                 <  1:822879/146‑1 (MQ=255)
                                       gCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCAt                                                                                                          <  1:69222/149‑1 (MQ=255)
                                       gCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCAt                                                                                                          <  4:476452/149‑1 (MQ=255)
                                         tttCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTc                                                                                                        >  2:420412/1‑149 (MQ=255)
                                         tttCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTc                                                                                                        >  3:664967/1‑149 (MQ=255)
                                           tCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCaa                                                                                                      >  3:109291/1‑149 (MQ=255)
                                               agccgagccgCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACTGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAAttt                                                                                                  >  1:802742/1‑149 (MQ=255)
                                               agccgagccgCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAAttt                                                                                                  >  3:429646/1‑149 (MQ=255)
                                                gccgagccgCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAAtttt                                                                                                 <  4:501009/149‑1 (MQ=255)
                                                   gagccgCGTTAAGCGTCAGGTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTaa                                                                                              >  3:765562/1‑149 (MQ=255)
                                                        gCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCtt                                                                                         >  2:935481/1‑149 (MQ=255)
                                                         cATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACATAGCATTCAAATTTTTAATGCttt                                                                                        <  4:112821/149‑1 (MQ=255)
                                                         cATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCttt                                                                                        <  2:428187/149‑1 (MQ=255)
                                                             aaGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTAttt                                                                                    <  1:930304/149‑1 (MQ=255)
                                                             aaGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTAttt                                                                                    <  1:294334/149‑1 (MQ=255)
                                                             aaGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTAttt                                                                                    <  2:383965/149‑1 (MQ=255)
                                                             aaGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTAttt                                                                                    <  1:727005/149‑1 (MQ=255)
                                                             aaGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTAttt                                                                                    <  4:234509/149‑1 (MQ=255)
                                                               gCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGc                                                                                  <  2:503190/149‑1 (MQ=255)
                                                                    aGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTttatt                                                                                                                     <  2:627049/109‑1 (MQ=255)
                                                                    aGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTttatt                                                                                                                     >  1:627049/1‑109 (MQ=255)
                                                                     gCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTtc                                                                            >  2:65493/1‑149 (MQ=255)
                                                                     gCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTtc                                                                            >  2:335237/1‑149 (MQ=255)
                                                                       ttCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTtctc                                                                          <  1:821333/149‑1 (MQ=255)
                                                                       ttCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTtctc                                                                          >  2:821333/1‑149 (MQ=255)
                                                                            ttGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGaa                                                                     <  4:429646/149‑1 (MQ=255)
                                                                                tgcGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTTGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTAc                                                                 <  1:14302/149‑1 (MQ=255)
                                                                                tgcGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTAc                                                                 >  3:174650/1‑149 (MQ=255)
                                                                                   gTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGaa                                                              >  3:347386/1‑149 (MQ=255)
                                                                                    ttATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGcc                                                                                 <  4:748404/129‑1 (MQ=255)
                                                                                    ttATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGcc                                                                                 >  3:748404/1‑129 (MQ=255)
                                                                                       tttACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGATGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAAAGCTTTATTTGCCATTTCTCCTGAATTACGAAAACa                                                          >  4:602454/1‑149 (MQ=255)
                                                                                        ttACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTAc                                                                 >  3:767153/1‑141 (MQ=255)
                                                                                        ttACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTAc                                                                 <  4:767153/141‑1 (MQ=255)
                                                                                         tACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACaat                                                        <  4:664967/149‑3 (MQ=255)
                                                                                           cGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTg                                                      <  2:749022/149‑1 (MQ=255)
                                                                                           cGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTg                                                      <  4:109291/149‑1 (MQ=255)
                                                                                              tGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCaa                                                   >  3:324770/1‑149 (MQ=255)
                                                                                              tGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCaa                                                   >  1:582644/1‑149 (MQ=255)
                                                                                                  aCTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACt                                               <  3:33593/149‑1 (MQ=255)
                                                                                                     acacCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGa                                            <  1:335237/149‑1 (MQ=255)
                                                                                                     acacCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGa                                            <  1:65493/149‑1 (MQ=255)
                                                                                                      cacCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGAt                                           <  2:665938/149‑1 (MQ=255)
                                                                                                         cGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGt                                                                                                                                                 <  3:722989/44‑1 (MQ=255)
                                                                                                         cGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGt                                                                                                                                                 >  4:722989/1‑44 (MQ=255)
                                                                                                         cGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTa                                        >  4:267485/1‑149 (MQ=255)
                                                                                                                 tATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAAc                                <  2:740965/149‑1 (MQ=255)
                                                                                                                 tATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAAc                                >  1:740965/1‑149 (MQ=255)
                                                                                                                 tATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAAc                                >  3:839516/1‑149 (MQ=255)
                                                                                                                  aTGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTtctc                                                                          >  1:423847/1‑106 (MQ=255)
                                                                                                                  aTGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTtctc                                                                          <  2:423847/106‑1 (MQ=255)
                                                                                                                   tGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGa                              >  4:185310/1‑149 (MQ=255)
                                                                                                                    gATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGAt                             <  1:935481/149‑1 (MQ=255)
                                                                                                                       tttCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATCACGATAAc                          <  2:395859/149‑1 (MQ=255)
                                                                                                                       tttCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAAc                          <  4:347386/149‑1 (MQ=255)
                                                                                                                       tttCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAAGGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAAc                          <  4:406579/149‑1 (MQ=255)
                                                                                                                       tctCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAAc                          <  4:765562/147‑1 (MQ=255)
                                                                                                                        ttCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAAcc                         >  2:133065/1‑149 (MQ=255)
                                                                                                                           ctctctTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGt                      >  1:659711/1‑149 (MQ=255)
                                                                                                                              tctTTGTAAAGCGACGGAGAGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGCATTACGAAAACATTTGCAACACTCGAGGTACCCATAACGATAACCGGTAac                   >  2:448514/1‑149 (MQ=255)
                                                                                                                               ctTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAATACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAaca                  >  3:82228/1‑149 (MQ=255)
                                                                                                                                tttGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAacac                 >  1:246542/1‑149 (MQ=255)
                                                                                                                                tttGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAacac                 >  2:82706/1‑149 (MQ=255)
                                                                                                                                    tAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGa             >  4:834449/1‑149 (MQ=255)
                                                                                                                                      aaGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGaaa           >  3:32853/1‑149 (MQ=255)
                                                                                                                                      aaGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGaaa           <  1:420412/149‑1 (MQ=255)
                                                                                                                                       aGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCg               >  4:205557/1‑144 (MQ=255)
                                                                                                                                       aGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCg               <  3:205557/144‑1 (MQ=255)
                                                                                                                                       aGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAg          >  3:253314/1‑149 (MQ=255)
                                                                                                                                         cGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCa        <  3:899516/149‑1 (MQ=255)
                                                                                                                                         cGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCa        <  4:839516/149‑1 (MQ=255)
                                                                                                                                         cGACGGAGTGACCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCa        >  3:856683/1‑149 (MQ=255)
                                                                                                                                          gACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCAt       >  1:377484/1‑149 (MQ=255)
                                                                                                                                          gACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCAt       <  2:520907/149‑1 (MQ=255)
                                                                                                                                          gACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCAt       <  3:185310/149‑1 (MQ=255)
                                                                                                                                          gACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCAt       <  4:174650/149‑1 (MQ=255)
                                                                                                                                          gACGGAGTGACGACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCAt       >  3:540249/1‑149 (MQ=255)
                                                                                                                                          gACGGAGTGACCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTTTCCTGAATTACGAAAACATTTGCAacac                                                <  4:627959/108‑1 (MQ=255)
                                                                                                                                          gACGGAGTGACCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATGTTTCCTGAATTACGAAAACATTTGCAacac                                                >  3:627959/1‑108 (MQ=255)
                                                                                                                                           aCGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGt                      >  4:627902/1‑133 (MQ=255)
                                                                                                                                           aCGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGt                      <  3:627902/133‑1 (MQ=255)
                                                                                                                                             ggAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCa    >  1:733972/1‑149 (MQ=255)
                                                                                                                                               aGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCaaa  >  2:382730/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AATCTGGCAAACGCTGGGGCTGGAGTGGCTCTACCGCCTGCTTTCGCAGCCGAGCCGCATTAAGCGTCAGCTTCGTTTGCTGCGTTATTTACGCTGGCACTACACCGGCAACCTATGATTTTCCTCTCTTTGTAAAGCGACGGAGTGGTCACTCCGTCGCCTGTCTGCTTTTTTATTACACAAAGCATTCAAATTTTTAATGCTTTATTTGCCATTTCTCCTGAATTACGAAAACATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAA  >  NC_000913/3980579‑3980870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: