Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,998,686 T→A 5.6% L235Q (CTG→CAG)  uvrD → DNA‑dependent ATPase I and helicase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,998,6860TA 5.6% 378.9 / 9.7 108L235Q (CTG→CAG) uvrDDNA‑dependent ATPase I and helicase II
Reads supporting (aligned to +/- strand):  ref base T (53/49);  new base A (2/4);  total (55/53)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

Only 100 of 113 total aligned reads displayed.

GTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGAAACTATTCGTCTGGAGCAAAACTACCGC  >  NC_000913/3998541‑3998834
                                                                                                                                                 |                                                                                                                                                    
gtggACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCctgct                                                                                                                                                   <  1:14883/149‑1 (MQ=255)
 tggACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCctgctg                                                                                                                                                  >  4:801772/1‑149 (MQ=255)
 tggACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCctgctg                                                                                                                                                  >  1:822627/1‑149 (MQ=255)
    aCTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTggcg                                                                                                                                               >  1:690982/1‑149 (MQ=255)
     cTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTggcgg                                                                                                                                              <  1:223339/149‑1 (MQ=255)
     cTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCAGCAggcgg                                                                                                                                              >  4:189033/1‑149 (MQ=255)
      ttCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTggcggg                                                                                                                                             >  3:484638/1‑149 (MQ=255)
       tCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCAGCAggcggat                                                                                                                                            <  3:189033/149‑3 (MQ=255)
       tCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCAGCAggcggat                                                                                                                                            <  1:513397/149‑3 (MQ=255)
          ccGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCAGCAggcggatcca                                                                                                                                         <  3:257209/149‑6 (MQ=255)
              gctgctgctGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCg                                                                                                                                     <  1:22584/149‑1 (MQ=255)
                 gctgctgcGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCa                                                                                                                                  >  1:353672/1‑149 (MQ=255)
                   tgctgcGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCaaa                                                                                                                                <  1:678024/149‑1 (MQ=255)
                   tgctgcGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCaaa                                                                                                                                >  4:408849/1‑149 (MQ=255)
                    gctgcGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAg                                                                                                                               <  1:658580/149‑1 (MQ=255)
                       gcgcgcTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGtga                                                                                                                            <  2:703625/149‑1 (MQ=255)
                        cgcgcTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGtgat                                                                                                                           >  1:931085/1‑149 (MQ=255)
                            cTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGAtc                                                                                                                       <  4:58742/149‑1 (MQ=255)
                            cTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGAtc                                                                                                                       <  3:628363/149‑1 (MQ=255)
                             tCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGGGACACCGGCAACGTGATGAtcg                                                                                                                      >  1:686986/1‑149 (MQ=255)
                             tCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGAtcg                                                                                                                      >  2:728090/1‑149 (MQ=255)
                             tCACGAGTTGTGGCTTAACAAGCCGCATAGCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGATGGCGGGCGACACCGGCAAAGTGATGAtcg                                                                                                                      >  3:752742/1‑149 (MQ=255)
                               aCGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGAtcgtc                                                                                                                    >  4:815696/1‑149 (MQ=255)
                                cGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGAtcgtcg                                                                                                                   <  1:408164/149‑1 (MQ=255)
                                  aGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGt                                                                                                                 <  2:166763/149‑1 (MQ=255)
                                   gTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGtg                                                                                                                <  2:855942/149‑1 (MQ=255)
                                    ttGTGGCTTAATAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGtga                                                                                                               >  3:490177/1‑149 (MQ=255)
                                    ttGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGtga                                                                                                               >  4:714433/1‑149 (MQ=255)
                                    ttGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGtga                                                                                                               <  4:726222/149‑1 (MQ=255)
                                      gtgGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGtgatg                                                                                                             <  4:540270/149‑1 (MQ=255)
                                        ggCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATgac                                                                                                           <  1:456830/149‑1 (MQ=255)
                                         gCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATgacg                                                                                                          >  4:896548/1‑149 (MQ=255)
                                          cTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATgacga                                                                                                         >  2:826884/1‑149 (MQ=255)
                                           ttAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATgacgac                                                                                                        >  2:816831/1‑149 (MQ=255)
                                           ttAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATgacgac                                                                                                        >  1:60718/1‑149 (MQ=255)
                                           ttAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATgacgac                                                                                                        >  2:563129/1‑149 (MQ=255)
                                           ttAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCACCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATgacgac                                                                                                        >  4:433492/1‑149 (MQ=255)
                                              aCAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAg                                                                                                     >  4:449008/1‑149 (MQ=255)
                                              aCAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAg                                                                                                     <  3:456388/149‑1 (MQ=255)
                                               cAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATTGTCGGTGATGACGACCAGt                                                                                                    >  4:552922/1‑149 (MQ=255)
                                               cAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGt                                                                                                    >  1:7949/1‑149 (MQ=255)
                                               cAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGt                                                                                                    >  1:727334/1‑149 (MQ=255)
                                                       atatCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTAc                                                                                            <  4:503926/149‑1 (MQ=255)
                                                              gCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTAc                                                                                            >  1:88298/1‑142 (MQ=255)
                                                                   acTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCggg                                                                                <  1:506810/149‑1 (MQ=255)
                                                                     tACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGgc                                                                              <  3:714433/149‑1 (MQ=255)
                                                                           gAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGt                                                                        <  2:690982/149‑1 (MQ=255)
                                                                            aaCGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTg                                                                       >  1:233599/1‑149 (MQ=255)
                                                                            aaCGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTg                                                                       >  4:261638/1‑149 (MQ=255)
                                                                              cGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGga                                                                     <  2:7949/149‑1 (MQ=255)
                                                                              cGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGga                                                                     <  4:54986/149‑1 (MQ=255)
                                                                                   tACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCACCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAAGCTACGGCTGGCGCGGGGCGCAGGGGGAGAata                                                                <  3:433492/149‑1 (MQ=255)
                                                                                    aCCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAatat                                                               >  4:393070/1‑149 (MQ=255)
                                                                                      cAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTc                                                             >  4:373015/1‑149 (MQ=255)
                                                                                      cAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTc                                                             >  4:583446/1‑149 (MQ=255)
                                                                                       aaTATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCa                                                            <  4:88249/149‑1 (MQ=255)
                                                                                        atatCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAg                                                           >  1:821992/1‑149 (MQ=255)
                                                                                         tatCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGc                                                          >  2:274322/1‑149 (MQ=255)
                                                                                           tCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAg                                                                          <  3:394861/131‑1 (MQ=255)
                                                                                             cTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGttt                                                      >  3:250583/1‑149 (MQ=255)
                                                                                             cTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGttt                                                      >  3:513617/1‑149 (MQ=255)
                                                                                               ggtggACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTcc                                                    <  1:563129/149‑1 (MQ=255)
                                                                                                   gACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGGTGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTaa                                                <  1:567641/149‑1 (MQ=255)
                                                                                                    aCGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAAt                                               <  3:696829/149‑1 (MQ=255)
                                                                                                    aCGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAAt                                               <  4:377538/149‑1 (MQ=255)
                                                                                                      gAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCaa                                                                                                                                 <  4:577439/65‑1 (MQ=255)
                                                                                                      gAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCaa                                                                                                                                 >  3:577439/1‑65 (MQ=255)
                                                                                                      gAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGc                                                          >  3:478714/1‑136 (MQ=255)
                                                                                                      gAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGc                                                          <  4:478714/136‑1 (MQ=255)
                                                                                                      gAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGa                                             <  2:822627/149‑1 (MQ=255)
                                                                                                        aTTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGAtt                                           >  3:349624/1‑149 (MQ=255)
                                                                                                         ttCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGAttt                                          >  4:35020/1‑149 (MQ=255)
                                                                                                         ttCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGAttt                                          <  4:484638/149‑1 (MQ=255)
                                                                                                         ttCAAGGATACAAACAAAATTCAGTACGCGTGGATCCGCCTGCTGGCAGGCGACACCGGCAAAGTGATCATCGTCGGTGATGACGACCAGTCACTCTACGGCTGGCGCGGGGCGCAGGTGCAGAATATTCAGCGTTTCCTTAATGAttt                                          >  2:899388/1‑149 (MQ=255)
                                                                                                           ccAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTcc                                        <  2:878954/149‑1 (MQ=255)
                                                                                                            cAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAat                                                                 >  2:561523/1‑123 (MQ=255)
                                                                                                            cAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAat                                                                 <  1:561523/123‑1 (MQ=255)
                                                                                                            cAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTcc                                                    <  4:655940/136‑1 (MQ=255)
                                                                                                            cAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTcc                                                    >  3:655940/1‑136 (MQ=255)
                                                                                                              ggATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCg                                     <  4:490177/149‑1 (MQ=255)
                                                                                                                     aacaacATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTg                                                                       <  2:34367/108‑1 (MQ=255)
                                                                                                                     aacaacATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTg                                                                       >  1:34367/1‑108 (MQ=255)
                                                                                                                     aacaacATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGa                              >  2:322800/1‑149 (MQ=255)
                                                                                                                     aacaacATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGa                              >  3:333105/1‑149 (MQ=255)
                                                                                                                     aacaacATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGa                              >  1:733494/1‑149 (MQ=255)
                                                                                                                     aacaacATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGa                              >  3:619540/1‑149 (MQ=255)
                                                                                                                     aacaacATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGa                              >  3:506205/1‑149 (MQ=255)
                                                                                                                      acaacaTTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGaa                             >  3:910360/1‑149 (MQ=255)
                                                                                                                       caacaTTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGaaa                            >  1:474762/1‑149 (MQ=255)
                                                                                                                       caacaTTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGaaa                            >  2:316040/1‑149 (MQ=255)
                                                                                                                        cacaTTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGAAAc                           <  1:826884/148‑1 (MQ=255)
                                                                                                                           aTTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAAt                                               <  4:753183/126‑1 (MQ=255)
                                                                                                                           aTTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAAt                                               >  3:753183/1‑126 (MQ=255)
                                                                                                                           aTTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGAAACTAt                        <  1:72267/149‑1 (MQ=255)
                                                                                                                            ttCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGga                                                                     >  1:426236/1‑103 (MQ=255)
                                                                                                                            ttCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGga                                                                     <  2:426236/103‑1 (MQ=255)
                                                                                                                                  aCGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGAAACTATTCGTCTg                 <  3:261638/149‑1 (MQ=255)
                                                                                                                                           tCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGAAACTATTCGTCTGGAGCAAAAc        <  4:619540/149‑1 (MQ=255)
                                                                                                                                              gCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGAAACTATTCGTCTGGAGCAAAACTAc     <  1:863987/149‑1 (MQ=255)
                                                                                                                                                 tgctgGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGAAACTATTCGTCTGGAGCAAAACTACCGc  <  4:506205/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGGCTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGACGAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACCGGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAGGTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGAAACTATTCGTCTGGAGCAAAACTACCGC  >  NC_000913/3998541‑3998834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: