Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,002,398 T→C 8.0% intergenic (+22/+21) corA → / ← yigF magnesium/nickel/cobalt transporter/DUF2628 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,002,3980TC 8.0% 319.8 / 12.9 100intergenic (+22/+21)corA/yigFmagnesium/nickel/cobalt transporter/DUF2628 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (40/52);  new base C (4/4);  total (44/56)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

TATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACAAATAGA  >  NC_000913/4002251‑4002545
                                                                                                                                                   |                                                                                                                                                   
tATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTc                                                                                                                                                    >  2:900703/1‑149 (MQ=255)
 aTGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCt                                                                                                                                                   <  2:83260/149‑1 (MQ=255)
    gCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGc                                                                                                                                                >  3:499812/1‑149 (MQ=255)
    gCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGc                                                                                                                                                <  4:339148/149‑1 (MQ=255)
      aTGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTa                                                                                                                                              >  2:917685/1‑149 (MQ=255)
      aTGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTa                                                                                                                                              >  4:854354/1‑149 (MQ=255)
       tGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTaa                                                                                                                                             <  4:443956/149‑1 (MQ=255)
         aaCTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGc                                                                                                                                           <  2:916756/149‑1 (MQ=255)
         aaCTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGc                                                                                                                                           >  1:264087/1‑149 (MQ=255)
           cTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCa                                                                                                                                         >  1:657607/1‑149 (MQ=255)
           cTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGCCAGACTAAGCCa                                                                                                                                         <  4:304874/149‑1 (MQ=255)
            tttGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCAc                                                                                                                                        <  4:29311/149‑1 (MQ=255)
            tttGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCAc                                                                                                                                        <  1:917685/149‑1 (MQ=255)
             ttGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCAcc                                                                                                                                       <  2:657607/149‑1 (MQ=255)
              tGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCt                                                                                                                                      <  2:363106/149‑1 (MQ=255)
                     aTGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTc                                                                                                                               <  2:609040/149‑1 (MQ=255)
                        ccAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCaaa                                                                                                                            <  4:312220/149‑1 (MQ=255)
                         cAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAg                                                                                                                           >  4:75852/1‑149 (MQ=255)
                         cAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAg                                                                                                                           >  2:541789/1‑149 (MQ=255)
                           gAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGc                                                                                                                         <  2:85129/149‑1 (MQ=255)
                              cTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTcc                                                                                                                      >  1:36525/1‑149 (MQ=255)
                              cTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTcc                                                                                                                      >  2:741007/1‑149 (MQ=255)
                                gAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAg                                                                                                                    <  1:27313/149‑1 (MQ=255)
                                gAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAg                                                                                                                    <  2:74895/149‑1 (MQ=255)
                                   gTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTa                                                                                                                 >  1:550215/1‑149 (MQ=255)
                                        gCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTaa                                                                                                            <  1:741007/149‑1 (MQ=255)
                                         cTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAAc                                                                                                           <  1:675349/149‑1 (MQ=255)
                                           tCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAAccc                                                                                                         <  2:462350/149‑1 (MQ=255)
                                                 aCCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTAc                                                                                                   <  2:303721/149‑1 (MQ=255)
                                                           gATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCa                                                                                         <  2:40029/149‑1 (MQ=255)
                                                           gATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCa                                                                                         <  2:218747/149‑1 (MQ=255)
                                                             ttATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATa                                                                                       >  3:204580/1‑149 (MQ=255)
                                                                 cTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAAc                                                                                   <  4:204580/149‑1 (MQ=255)
                                                                  tttATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTcc                                                                                                                      <  2:64493/113‑1 (MQ=255)
                                                                  tttATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTcc                                                                                                                      >  1:64493/1‑113 (MQ=255)
                                                                  tttATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAAcc                                                                                  >  3:806859/1‑149 (MQ=255)
                                                                  tttATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAAcc                                                                                  >  1:506512/1‑149 (MQ=255)
                                                                  gttATGGTCCTCGCGGGCCTGGCACCGTTACAGTACTTTAGGCGGACGAATTGGTTGTTACAAACGCGAGAGGTGGCATTGTCAGGGTAAGTCACGTGTTTTGCAAAGCCTACAGGTATTTAATGTTTTTTCCTCTTTTTAGTAGaaaa                                                                                  >  4:567519/2‑147 (MQ=255)
                                                                    tATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCAt                                                                                >  3:94539/1‑149 (MQ=255)
                                                                         tCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGtt                                                                           >  2:175509/1‑149 (MQ=255)
                                                                           cTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGCCAGACTAAGCCAc                                                                                                                                        >  3:131510/1‑86 (MQ=39)
                                                                           cTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGCCAGACTAAGCCAc                                                                                                                                        <  4:131510/86‑1 (MQ=39)
                                                                            tCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCgt                                                                        <  1:529783/149‑1 (MQ=255)
                                                                            tCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCgt                                                                        <  2:858454/149‑1 (MQ=255)
                                                                            tCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCgt                                                                        >  1:409875/1‑149 (MQ=255)
                                                                                 ggCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTaa                                                                   <  3:288256/149‑1 (MQ=255)
                                                                                 ggCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTaa                                                                   <  1:507489/149‑1 (MQ=255)
                                                                                 ggCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTaa                                                                   >  2:305321/1‑149 (MQ=255)
                                                                                  gCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAAc                                                                  <  3:917306/149‑1 (MQ=255)
                                                                                   ccTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACa                                                                 <  4:87278/149‑1 (MQ=255)
                                                                                    cTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAg                                                                >  4:501515/1‑149 (MQ=255)
                                                                                       gCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCaa                                                             <  1:923306/149‑1 (MQ=255)
                                                                                       gCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCaa                                                             <  2:656617/149‑1 (MQ=255)
                                                                                        cACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAAc                                                            <  1:790789/149‑1 (MQ=255)
                                                                                         aCCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAAc                                                                                                           >  2:869109/1‑101 (MQ=255)
                                                                                         aCCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAAc                                                                                                           <  1:869109/101‑1 (MQ=255)
                                                                                         aCCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACa                                                           <  1:392324/149‑1 (MQ=255)
                                                                                          ccGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACAt                                                          <  3:75852/149‑1 (MQ=255)
                                                                                           tgTATCTGTACTTTATGCAGAACAACTGGTGGTAAAAAACGAGAGCGGTGGCTTAGTCGGGCTAAGCCTCCTGTTATTCAAAGTCTCCAGGTATTTAACCCTATTACCTCTTTCGCATAGAACCATTTGTTCGTGTTAACAGCAACATa                                                         <  3:567519/148‑1 (MQ=255)
                                                                                            gTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAg                                                        >  1:103675/1‑149 (MQ=255)
                                                                                            gTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAg                                                        >  3:36016/1‑149 (MQ=255)
                                                                                               tCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGgct                                                     <  2:103675/149‑1 (MQ=255)
                                                                                               tCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGgct                                                     <  4:36016/149‑1 (MQ=255)
                                                                                                 tgtgCTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGgctgc                                                   >  3:213309/1‑149 (MQ=255)
                                                                                                      tttAAGCGGAAGAACTGGTTGTAGAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACgg                                              >  1:158239/1‑149 (MQ=255)
                                                                                                      tttAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACgg                                              >  4:324074/1‑149 (MQ=255)
                                                                                                                   aCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAAt                                 <  1:175509/149‑1 (MQ=255)
                                                                                                                     tGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGaa                                                                                    >  1:497761/1‑96 (MQ=255)
                                                                                                                     tGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGaa                                                                                    <  2:497761/96‑1 (MQ=255)
                                                                                                                     tGGTTGTAAAAAACGAGAGCGGTGGCTTAGCCAGACTaa                                                                                                                                             >  1:448839/1‑39 (MQ=21)
                                                                                                                     tGGTTGTAAAAAACGAGAGCGGTGGCTTAGCCAGACTaa                                                                                                                                             <  2:448839/39‑1 (MQ=21)
                                                                                                                        ttGTAGAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTaa                            <  2:158239/149‑1 (MQ=255)
                                                                                                                        ttGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAgg                                                       >  4:384458/1‑122 (MQ=255)
                                                                                                                        ttGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAgg                                                       <  3:905690/122‑1 (MQ=255)
                                                                                                                        ttGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAgg                                                       >  4:905690/1‑122 (MQ=255)
                                                                                                                        ttGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAgg                                                       <  3:384458/122‑1 (MQ=255)
                                                                                                                        ttGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTaa                            <  2:506512/149‑1 (MQ=255)
                                                                                                                        ttGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTaa                            <  1:748064/149‑1 (MQ=255)
                                                                                                                         tGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAAc                           <  4:806859/149‑1 (MQ=255)
                                                                                                                           tAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGc                         >  1:103594/1‑149 (MQ=255)
                                                                                                                           tAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGc                         >  4:710095/1‑149 (MQ=255)
                                                                                                                            aaaaaaCGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGcc                        >  1:58002/1‑149 (MQ=255)
                                                                                                                             aaaaaCGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGt                               >  1:111176/1‑141 (MQ=255)
                                                                                                                             aaaaaCGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGt                               <  2:111176/141‑1 (MQ=255)
                                                                                                                               aaaCGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAAt                     >  3:695153/1‑149 (MQ=255)
                                                                                                                                 aCGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTCTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTa                   <  2:68693/149‑1 (MQ=255)
                                                                                                                                   gagaGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGt                 <  2:550215/149‑1 (MQ=255)
                                                                                                                                    taacaggTGGCTTAGCCAGACTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGt                 >  3:80792/6‑148 (MQ=255)
                                                                                                                                    taacaggTGGCTTAGCCAGACTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGt                 <  4:80792/143‑1 (MQ=255)
                                                                                                                                       gCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCAc             <  3:854354/149‑1 (MQ=255)
                                                                                                                                          gTGGCTTAGCCAGACTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACcaa          >  4:672943/1‑149 (MQ=255)
                                                                                                                                           tGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACcaac         >  2:259298/1‑149 (MQ=255)
                                                                                                                                              cTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTTCACCAACaaa      <  2:375451/149‑1 (MQ=255)
                                                                                                                                              cTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACaaa      <  1:259298/149‑1 (MQ=255)
                                                                                                                                               ttAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACAAAt     >  2:841621/1‑149 (MQ=255)
                                                                                                                                               ttAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACAAAt     >  3:492650/1‑149 (MQ=255)
                                                                                                                                               ttAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACAAAt     >  3:638075/1‑149 (MQ=255)
                                                                                                                                                  gTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACa                                                                 >  1:849070/1‑86 (MQ=255)
                                                                                                                                                  gTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACa                                                                 <  2:849070/86‑1 (MQ=255)
                                                                                                                                                  gTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACAAATaga  <  1:495524/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACAAATAGA  >  NC_000913/4002251‑4002545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑