Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,002,400 T→A 9.0% intergenic (+24/+19) corA → / ← yigF magnesium/nickel/cobalt transporter/DUF2628 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,002,4000TA 9.0% 337.6 / 16.7 99intergenic (+24/+19)corA/yigFmagnesium/nickel/cobalt transporter/DUF2628 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (38/51);  new base A (5/4);  total (43/56)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

ATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACAAATAGAGAT  >  NC_000913/4002252‑4002548
                                                                                                                                                    |                                                                                                                                                    
aTGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCt                                                                                                                                                      <  2:83260/149‑1 (MQ=255)
   gCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGc                                                                                                                                                   >  3:499812/1‑149 (MQ=255)
   gCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGc                                                                                                                                                   <  4:339148/149‑1 (MQ=255)
     aTGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTa                                                                                                                                                 >  4:854354/1‑149 (MQ=255)
     aTGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTa                                                                                                                                                 >  2:917685/1‑149 (MQ=255)
      tGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTaa                                                                                                                                                <  4:443956/149‑1 (MQ=255)
        aaCTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGc                                                                                                                                              <  2:916756/149‑1 (MQ=255)
        aaCTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGc                                                                                                                                              >  1:264087/1‑149 (MQ=255)
          cTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCa                                                                                                                                            >  1:657607/1‑149 (MQ=255)
          cTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGCCAGACTAAGCCa                                                                                                                                            <  4:304874/149‑1 (MQ=255)
           tttGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCAc                                                                                                                                           <  1:917685/149‑1 (MQ=255)
           tttGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCAc                                                                                                                                           <  4:29311/149‑1 (MQ=255)
            ttGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCAcc                                                                                                                                          <  2:657607/149‑1 (MQ=255)
             tGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCt                                                                                                                                         <  2:363106/149‑1 (MQ=255)
                    aTGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTc                                                                                                                                  <  2:609040/149‑1 (MQ=255)
                       ccAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCaaa                                                                                                                               <  4:312220/149‑1 (MQ=255)
                        cAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAg                                                                                                                              >  2:541789/1‑149 (MQ=255)
                        cAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAg                                                                                                                              >  4:75852/1‑149 (MQ=255)
                          gAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGc                                                                                                                            <  2:85129/149‑1 (MQ=255)
                             cTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTcc                                                                                                                         >  1:36525/1‑149 (MQ=255)
                             cTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTcc                                                                                                                         >  2:741007/1‑149 (MQ=255)
                               gAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAg                                                                                                                       <  1:27313/149‑1 (MQ=255)
                               gAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAg                                                                                                                       <  2:74895/149‑1 (MQ=255)
                                  gTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTa                                                                                                                    >  1:550215/1‑149 (MQ=255)
                                       gCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTaa                                                                                                               <  1:741007/149‑1 (MQ=255)
                                        cTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAAc                                                                                                              <  1:675349/149‑1 (MQ=255)
                                          tCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAAccc                                                                                                            <  2:462350/149‑1 (MQ=255)
                                                aCCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTAc                                                                                                      <  2:303721/149‑1 (MQ=255)
                                                          gATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCa                                                                                            <  2:40029/149‑1 (MQ=255)
                                                          gATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCa                                                                                            <  2:218747/149‑1 (MQ=255)
                                                            ttATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATa                                                                                          >  3:204580/1‑149 (MQ=255)
                                                                cTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAAc                                                                                      <  4:204580/149‑1 (MQ=255)
                                                                 tttATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTcc                                                                                                                         >  1:64493/1‑113 (MQ=255)
                                                                 tttATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTcc                                                                                                                         <  2:64493/113‑1 (MQ=255)
                                                                 tttATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAAcc                                                                                     >  3:806859/1‑149 (MQ=255)
                                                                 tttATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAAcc                                                                                     >  1:506512/1‑149 (MQ=255)
                                                                 gttATGGTCCTCGCGGGCCTGGCACCGTTACAGTACTTTAGGCGGACGAATTGGTTGTTACAAACGCGAGAGGTGGCATTGTCAGGGTAAGTCACGTGTTTTGCAAAGCCTACAGGTATTTAATGTTTTTTCCTCTTTTTAGTAGaaaa                                                                                     >  4:567519/2‑147 (MQ=255)
                                                                   tATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCAt                                                                                   >  3:94539/1‑149 (MQ=255)
                                                                        tCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGtt                                                                              >  2:175509/1‑149 (MQ=255)
                                                                          cTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGCCAGACTAAGCCAc                                                                                                                                           >  3:131510/1‑86 (MQ=39)
                                                                          cTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGCCAGACTAAGCCAc                                                                                                                                           <  4:131510/86‑1 (MQ=39)
                                                                           tCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCgt                                                                           <  2:858454/149‑1 (MQ=255)
                                                                           tCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCgt                                                                           <  1:529783/149‑1 (MQ=255)
                                                                           tCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCgt                                                                           >  1:409875/1‑149 (MQ=255)
                                                                                ggCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTaa                                                                      >  2:305321/1‑149 (MQ=255)
                                                                                ggCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTaa                                                                      <  3:288256/149‑1 (MQ=255)
                                                                                ggCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTaa                                                                      <  1:507489/149‑1 (MQ=255)
                                                                                 gCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAAc                                                                     <  3:917306/149‑1 (MQ=255)
                                                                                  ccTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACa                                                                    <  4:87278/149‑1 (MQ=255)
                                                                                   cTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAg                                                                   >  4:501515/1‑149 (MQ=255)
                                                                                      gCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCaa                                                                <  2:656617/149‑1 (MQ=255)
                                                                                      gCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCaa                                                                <  1:923306/149‑1 (MQ=255)
                                                                                       cACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAAc                                                               <  1:790789/149‑1 (MQ=255)
                                                                                        aCCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAAc                                                                                                              >  2:869109/1‑101 (MQ=255)
                                                                                        aCCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAAc                                                                                                              <  1:869109/101‑1 (MQ=255)
                                                                                        aCCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACa                                                              <  1:392324/149‑1 (MQ=255)
                                                                                         ccGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACAt                                                             <  3:75852/149‑1 (MQ=255)
                                                                                          tgTATCTGTACTTTATGCAGAACAACTGGTGGTAAAAAACGAGAGCGGTGGCTTAGTCGGGCTAAGCCTCCTGTTATTCAAAGTCTCCAGGTATTTAACCCTATTACCTCTTTCGCATAGAACCATTTGTTCGTGTTAACAGCAACATa                                                            <  3:567519/148‑1 (MQ=255)
                                                                                           gTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAg                                                           >  1:103675/1‑149 (MQ=255)
                                                                                           gTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAg                                                           >  3:36016/1‑149 (MQ=255)
                                                                                              tCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGgct                                                        <  4:36016/149‑1 (MQ=255)
                                                                                              tCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGgct                                                        <  2:103675/149‑1 (MQ=255)
                                                                                                tgtgCTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGgctgc                                                      >  3:213309/1‑149 (MQ=255)
                                                                                                     tttAAGCGGAAGAACTGGTTGTAGAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACgg                                                 >  1:158239/1‑149 (MQ=255)
                                                                                                     tttAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACgg                                                 >  4:324074/1‑149 (MQ=255)
                                                                                                                  aCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAAt                                    <  1:175509/149‑1 (MQ=255)
                                                                                                                    tGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGaa                                                                                       >  1:497761/1‑96 (MQ=255)
                                                                                                                    tGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGaa                                                                                       <  2:497761/96‑1 (MQ=255)
                                                                                                                    tGGTTGTAAAAAACGAGAGCGGTGGCTTAGCCAGACTaa                                                                                                                                                <  2:448839/39‑1 (MQ=21)
                                                                                                                    tGGTTGTAAAAAACGAGAGCGGTGGCTTAGCCAGACTaa                                                                                                                                                >  1:448839/1‑39 (MQ=21)
                                                                                                                       ttGTAGAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTaa                               <  2:158239/149‑1 (MQ=255)
                                                                                                                       ttGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAgg                                                          <  3:384458/122‑1 (MQ=255)
                                                                                                                       ttGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAgg                                                          >  4:384458/1‑122 (MQ=255)
                                                                                                                       ttGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAgg                                                          <  3:905690/122‑1 (MQ=255)
                                                                                                                       ttGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTaa                               <  2:506512/149‑1 (MQ=255)
                                                                                                                       ttGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTaa                               <  1:748064/149‑1 (MQ=255)
                                                                                                                        tGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAAc                              <  4:806859/149‑1 (MQ=255)
                                                                                                                          tAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGc                            >  1:103594/1‑149 (MQ=255)
                                                                                                                          tAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGc                            >  4:710095/1‑149 (MQ=255)
                                                                                                                           aaaaaaCGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGcc                           >  1:58002/1‑149 (MQ=255)
                                                                                                                            aaaaaCGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGt                                  <  2:111176/141‑1 (MQ=255)
                                                                                                                            aaaaaCGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGt                                  >  1:111176/1‑141 (MQ=255)
                                                                                                                              aaaCGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAAt                        >  3:695153/1‑149 (MQ=255)
                                                                                                                                aCGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTCTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTa                      <  2:68693/149‑1 (MQ=255)
                                                                                                                                  gagaGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGt                    <  2:550215/149‑1 (MQ=255)
                                                                                                                                   taacaggTGGCTTAGCCAGACTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGt                    >  3:80792/6‑148 (MQ=255)
                                                                                                                                   taacaggTGGCTTAGCCAGACTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGt                    <  4:80792/143‑1 (MQ=255)
                                                                                                                                      gCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCAc                <  3:854354/149‑1 (MQ=255)
                                                                                                                                         gTGGCTTAGCCAGACTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACcaa             >  4:672943/1‑149 (MQ=255)
                                                                                                                                          tGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACcaac            >  2:259298/1‑149 (MQ=255)
                                                                                                                                             cTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTTCACCAACaaa         <  2:375451/149‑1 (MQ=255)
                                                                                                                                             cTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACaaa         <  1:259298/149‑1 (MQ=255)
                                                                                                                                              ttAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACAAAt        >  3:638075/1‑149 (MQ=255)
                                                                                                                                              ttAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACAAAt        >  3:492650/1‑149 (MQ=255)
                                                                                                                                              ttAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACAAAt        >  2:841621/1‑149 (MQ=255)
                                                                                                                                                 gTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACa                                                                    >  1:849070/1‑86 (MQ=255)
                                                                                                                                                 gTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACa                                                                    <  2:849070/86‑1 (MQ=255)
                                                                                                                                                 gTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACAAATaga     <  1:495524/149‑1 (MQ=255)
                                                                                                                                                   cTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACAAATAgaga   <  1:9131/149‑1 (MQ=255)
                                                                                                                                                    tGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACAAATAGAGAt  <  2:103594/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGCAATTCCCAGAATGTTAACGCCAATTAGTGCACCAACAAATAGAGAT  >  NC_000913/4002252‑4002548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: