Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,040,660 G→T 6.3% intergenic (+24/+246) rrfA → / ← mobB 5S ribosomal RNA of rrnA operon/molybdopterin‑guanine dinucleotide biosynthesis protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,040,6600GT 6.3% 299.7 / 6.7 92intergenic (+24/+246)rrfA/mobB5S ribosomal RNA of rrnA operon/molybdopterin‑guanine dinucleotide biosynthesis protein B
Reads supporting (aligned to +/- strand):  ref base G (44/41);  new base T (2/4);  total (46/46)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATC  >  NC_000913/4040514‑4040805
                                                                                                                                                  |                                                                                                                                                 
aTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGa                                                                                                                                                 >  3:277377/1‑149 (MQ=34)
 tttGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCCTGCGAGAGTAGGGAACTGCCAGGCATCAAATAAATCGAAAGCCTCATTCCGaa                                                                                                                                                <  2:637123/149‑1 (MQ=11)
 tttGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGaa                                                                                                                                                <  2:421032/149‑1 (MQ=34)
      cTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTg                                                                                                                                           <  1:350420/149‑1 (MQ=35)
       tGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTgg                                                                                                                                          <  4:165822/149‑1 (MQ=35)
         gcggcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGc                                                                                                                                        <  3:198409/149‑1 (MQ=35)
               gTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCg                                                                                                                                  >  4:808655/1‑149 (MQ=34)
                 aGCGCGGTGGTCCCTCCTGACACCATGCCGAACTCAGAAGTGAAACGCTGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGtt                                                                                                                                <  1:243157/149‑1 (MQ=255)
                         ggtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTCGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATttga                                                                                                                        <  1:667890‑M1/149‑5 (MQ=255)
                            cccACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCtgttgt                                                                                                                     <  2:132796/149‑1 (MQ=35)
                             ccACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTgttgtt                                                                                                                    <  3:860849/149‑1 (MQ=35)
                              cACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGttgttt                                                                                                                   <  2:677963/149‑1 (MQ=35)
                              cACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGttgttt                                                                                                                   <  4:631904/149‑1 (MQ=35)
                               aCCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTAT                                                                                                                            >  4:724176‑M1/1‑139 (MQ=255)
                               aCCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTAT                                                                                                                            <  3:724176‑M1/139‑1 (MQ=255)
                                 cTGAACCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTc                                                                                                                <  3:775798/149‑1 (MQ=21)
                                       ccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGaa                                                                                                          >  4:559461/1‑149 (MQ=35)
                                       ccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGaa                                                                                                          <  1:61544/149‑1 (MQ=34)
                                             cGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGctct                                                                                                    <  4:109696/149‑1 (MQ=35)
                                              gAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTc                                                                                                                                   >  4:683673/1‑117 (MQ=38)
                                              gAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTc                                                                                                                                   <  3:683673/117‑1 (MQ=38)
                                                aCTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCt                                                                                                 <  4:681717/149‑1 (MQ=35)
                                                  taagaAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATttgatg                                                                                                                      <  3:110049‑M1/124‑7 (MQ=255)
                                                  tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGa                                                                                               <  3:407040/149‑1 (MQ=35)
                                                  tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGa                                                                                               >  2:906720/1‑149 (MQ=34)
                                                  tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATttgatg                                                                                                                      >  4:110049‑M1/1‑120 (MQ=255)
                                                   cAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAg                                                                                              <  4:277377/149‑1 (MQ=35)
                                                    aGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGc                                                                                                       >  4:640937/1‑139 (MQ=35)
                                                    aGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGc                                                                                                       <  3:640937/139‑1 (MQ=37)
                                                     gAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTa                                                                                            >  3:907890/1‑149 (MQ=34)
                                                      aaGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCTAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAg                                                                                           <  4:665600/149‑1 (MQ=25)
                                                      aaGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAg                                                                                           <  4:907890/149‑1 (MQ=35)
                                                        gTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATt                                                                                                                           >  1:817728‑M1/1‑114 (MQ=255)
                                                        gTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATt                                                                                                                           <  2:817728‑M1/115‑2 (MQ=255)
                                                         tGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAg                                                                                           <  1:873786/146‑1 (MQ=34)
                                                         tGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAg                                                                                           >  2:873786/1‑146 (MQ=35)
                                                           aaaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACaa                                                                                      >  2:302215/1‑149 (MQ=35)
                                                            aaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACaaa                                                                                     <  3:308154/149‑1 (MQ=35)
                                                                  gTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATccgcc                                                                               <  3:808655/149‑1 (MQ=35)
                                                                   tAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATccgctc                                                                              >  1:264053/1‑147 (MQ=25)
                                                                          gATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCgg                                                                       >  1:812568/1‑149 (MQ=35)
                                                                             ggTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGAtt                                                                    <  2:812568/149‑1 (MQ=34)
                                                                              gTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGAttt                                                                   >  2:783468/1‑149 (MQ=34)
                                                                                     ggggTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGt                                                                                                                                 <  3:442224/80‑1 (MQ=40)
                                                                                     ggggTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGt                                                                                                                                 >  4:442224/1‑80 (MQ=255)
                                                                                      gggTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAAc                                                               <  4:670683/145‑1 (MQ=34)
                                                                                      gggTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAAc                                                               >  3:670683/1‑145 (MQ=34)
                                                                                      gggTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTg                                                           <  3:559461/149‑1 (MQ=35)
                                                                                          ctcctcATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGaa                                                       <  4:830836/149‑1 (MQ=35)
                                                                                          ctcctcATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGaa                                                       <  2:175926/149‑1 (MQ=35)
                                                                                             ctcATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGc                                                          >  2:62703/1‑143 (MQ=34)
                                                                                             ctcATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGc                                                          <  1:62703/143‑1 (MQ=34)
                                                                                             ctcATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCa                                                    >  4:546944/1‑149 (MQ=33)
                                                                                             ctcATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCa                                                    >  1:800703/1‑149 (MQ=34)
                                                                                              tcATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCTGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGATCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCaa                                                   <  4:582063/149‑1 (MQ=18)
                                                                                                   cGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCAAAGCAACGGcc                                              <  1:693782/149‑1 (MQ=17)
                                                                                                    gagaGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGccc                                             >  2:893605/1‑149 (MQ=32)
                                                                                                       agTAGGGAACTGCCAGGCATCAAATAAAACGAAGGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGa                                          <  1:893605/149‑1 (MQ=17)
                                                                                                          aGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGcctggcagt                                       >  1:332440/1‑142 (MQ=17)
                                                                                                          aGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGCCGGAAGACTGGGCCTTTCGTTTTATCTATTGTTTGTCGGTGAACGCTCTCCTGAGTAGGTCAAATACGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAggg                                       >  3:776339/1‑149 (MQ=255)
                                                                                                               aCTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCg                                  <  1:896545/149‑1 (MQ=32)
                                                                                                                   ccAGGCATCAAATAAAACGAAAGGCACAGGCGGAAGGCTGGGCTTTTCGTTTTATCTGTGGTTTGTCGGCGAACGCTCTCTTGAGTAGGACAACTCCGCCGGGAGCGGATTTGAACGTTGCGAAACACCGGCCCGGAGGGTGGCGGCCa                              <  4:776339/149‑1 (MQ=11)
                                                                                                                          tCAAATAAATCGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCGGGAGGGTGGCGGGCAGGAcgcc                       >  1:802715/1‑149 (MQ=17)
                                                                                                                            aaaTAAAACAAAAGGCTCAGTCGGAAGACTGGGCCTTTTGTTTTATttgatgtctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccac                     >  1:172811‑M1/1‑46 (MQ=255)
                                                                                                                             aaTAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGAcgcccgc                    >  4:394332/1‑149 (MQ=32)
                                                                                                                                  aacaaaaggctCAGTCGGAAGACTGGGCCTTTTGTTTTATttgatgtctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggg                                    <  4:286644‑M1/124‑89 (MQ=255)
                                                                                                                                  aaCAAAAGGCTCAGTCGGAAGACTGGGCCTTTTGTTTTATttgatgtctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggg                                    >  3:286644‑M1/1‑40 (MQ=255)
                                                                                                                                   aCGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatgaggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgcta              >  3:115762‑M1/1‑39 (MQ=255)
                                                                                                                                   aCGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatgaggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgcta              >  3:138307‑M1/1‑39 (MQ=255)
                                                                                                                                    caaaaggCTCAGTCGGAAGACTGGGCCTTTTGTTTTATttgatgtctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctac             >  1:915517‑M1/3‑38 (MQ=255)
                                                                                                                                     gAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACt            <  3:546944/149‑1 (MQ=32)
                                                                                                                                     gAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACt            >  1:553614/1‑149 (MQ=32)
                                                                                                                                     gAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACt            >  1:173346/1‑149 (MQ=32)
                                                                                                                                     aaaagGCTCAGTCGGAAGACTGGGCCTTTTGTTTTATttgatgtctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctact            >  1:245092‑M1/2‑37 (MQ=255)
                                                                                                                                       aaGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatgaggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgc          >  3:401982‑M1/1‑35 (MQ=255)
                                                                                                                                        aGGCTCAGTCGGAAGACTGGGCCTTTTGTTTTATttgatgtctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgcc         >  3:437560‑M1/1‑34 (MQ=255)
                                                                                                                                        aGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatgaggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgcc         >  4:557897‑M1/1‑34 (MQ=255)
                                                                                                                                        aGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgcc         >  4:868984‑M1/1‑34 (MQ=255)
                                                                                                                                        aGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgttt                                                         <  2:559288‑M1/101‑68 (MQ=255)
                                                                                                                                        aGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgttt                                                         >  1:559288‑M1/1‑34 (MQ=255)
                                                                                                                                         ggCTCAGTCGGAAGACTGGGCCTTTTGTTTTATttgatgtctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgttt                                                         >  1:581489‑M1/1‑33 (MQ=255)
                                                                                                                                         ggCTCAGTCGGAAGACTGGGCCTTTTGTTTTATttgatgtctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgttt                                                         <  2:581489‑M1/100‑68 (MQ=255)
                                                                                                                                         ggCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCa        <  2:553614/149‑1 (MQ=32)
                                                                                                                                         ggCTCAGTCGAAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccg        >  1:57058‑M1/1‑33 (MQ=255)
                                                                                                                                         ggCTCAGTCGAAAGACTGGGCCTTTCGGTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccg        >  4:444095‑M1/1‑33 (MQ=255)
                                                                                                                                          gCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAg       >  4:558706/1‑149 (MQ=32)
                                                                                                                                            tCAGTCGGAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatgaggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgcca     <  3:557897‑M1/149‑120 (MQ=255)
                                                                                                                                            tCAGTCGGAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatgaggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgcca     >  3:136958‑M1/1‑30 (MQ=255)
                                                                                                                                            tCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGc     >  4:615937/1‑149 (MQ=32)
                                                                                                                                             cAGTCGGAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatgaggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgccag    <  4:136958‑M1/149‑121 (MQ=255)
                                                                                                                                               gTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATc  >  2:191939/1‑149 (MQ=32)
                                                                                                                                               gTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATc  >  1:937352/1‑149 (MQ=32)
                                                                                                                                                  |                                                                                                                                                 
ATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATC  >  NC_000913/4040514‑4040805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: