Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,366,854 T→G 13.1% intergenic (‑81/+37) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,366,8540TG13.1% 234.5 / 29.3 83intergenic (‑81/+37)dcuA/aspAC4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base T (38/34);  new base G (7/4);  total (45/38)
Fisher's exact test for biased strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-01

CCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGTTCC  >  NC_000913/4366706‑4366999
                                                                                                                                                    |                                                                                                                                                 
ccTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGCAAAAAGGCACGTCAt                                                                                                                                                   >  1:875193/1‑149 (MQ=255)
 cTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATc                                                                                                                                                  >  1:829609/1‑149 (MQ=255)
 cTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATc                                                                                                                                                  <  1:905482/149‑1 (MQ=255)
 cTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATc                                                                                                                                                  <  4:788722/149‑1 (MQ=255)
  tATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCt                                                                                                                                                 <  1:453856/149‑1 (MQ=255)
    ttCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGa                                                                                                                                               >  4:734853/1‑149 (MQ=255)
    ttCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGa                                                                                                                                               <  3:598472/149‑1 (MQ=255)
        ccccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTg                                                                                                                                           >  3:523293/1‑149 (MQ=255)
         cccAATCTGTCACCCAAGAAGATCTCCAGCAAAACTATGATGAGTTCTACAACTAGCTTATTAGCCTTCCTGGTTTTTTAACAAGTTGAAATAAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGc                                                                                                                                          >  4:516188/1‑149 (MQ=255)
         cccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGc                                                                                                                                          <  4:523293/149‑1 (MQ=255)
           cattCTGGCGCCCAATAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTTTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGATACTGCTCACTAGAAAAAAGGCACGTCAGATCACGTGCCt                                                                                                                                        <  2:337743/146‑1 (MQ=255)
               cTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCttttt                                                                                                                                    <  3:392726/149‑1 (MQ=255)
               cTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGCCttttt                                                                                                                                    <  1:6825/149‑1 (MQ=255)
                tGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAGGGCACGTCATCTGACGt                                                                                                                                            >  4:29586/1‑140 (MQ=255)
                tGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAGGGCACGTCATCTGACGt                                                                                                                                            <  3:29586/140‑1 (MQ=255)
                 ggCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTa                                                                                                                                  <  2:573391/149‑1 (MQ=255)
                  gcgcCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTAt                                                                                                                                 <  2:889513/149‑1 (MQ=255)
                      ccAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGt                                                                                                                             >  2:602903/1‑149 (MQ=255)
                      ccAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGt                                                                                                                             >  2:90323/1‑149 (MQ=255)
                      ccAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGt                                                                                                                             <  3:747124/149‑1 (MQ=255)
                       cAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGta                                                                                                                            >  4:739674/1‑149 (MQ=255)
                        aagaagATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCttt                                                                                                                                      >  3:149709/1‑138 (MQ=255)
                        aagaagATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCttt                                                                                                                                      <  4:149709/138‑1 (MQ=255)
                              aTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCt                                                                                                                     >  2:559511/1‑149 (MQ=255)
                              aTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCt                                                                                                                     <  2:829609/149‑1 (MQ=255)
                                         aaCTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACt                                                                                                          <  4:917320/149‑1 (MQ=255)
                                                  gaGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCttt                                                                                                 <  3:737141/149‑1 (MQ=255)
                                                   aGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTTCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtc                                                                                                <  3:362387/149‑1 (MQ=255)
                                                     ttCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcat                                                                                              >  1:220328/1‑149 (MQ=255)
                                                     ttCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcat                                                                                              >  2:489209/1‑149 (MQ=255)
                                                     ttCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGc                                                                                                                                          <  1:576500/105‑1 (MQ=255)
                                                     ttCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGc                                                                                                                                          >  2:576500/1‑105 (MQ=255)
                                                       cTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcatca                                                                                            <  1:559511/149‑1 (MQ=255)
                                                        tACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCttt                                                                                                                                      <  4:27791/106‑1 (MQ=255)
                                                        tACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCttt                                                                                                                                      >  3:27791/1‑106 (MQ=255)
                                                        tACAACGAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAg                                                                                           >  2:456896/1‑149 (MQ=255)
                                                         aCAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGt                                                                                          >  4:391560/1‑149 (MQ=255)
                                                                gCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTCTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTAttt                                                                                                                               <  2:300494/105‑1 (MQ=255)
                                                                gCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTCTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTAttt                                                                                                                               >  1:300494/1‑105 (MQ=255)
                                                                     ttAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGc                                                                              >  3:634443/1‑149 (MQ=255)
                                                                      tAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCt                                                                             <  3:734853/149‑1 (MQ=255)
                                                                       aGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCtt                                                                            <  1:90323/149‑1 (MQ=255)
                                                                        gCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTAcc                                                                                                                       >  2:884380/1‑105 (MQ=255)
                                                                        gCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTAcc                                                                                                                       <  1:884380/105‑1 (MQ=255)
                                                                         ccttccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTg                                                                          >  2:280354/1‑149 (MQ=255)
                                                                          cttccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGt                                                                         >  3:210240/1‑149 (MQ=255)
                                                                          cttccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGt                                                                         >  1:807252/1‑149 (MQ=255)
                                                                           ttccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTa                                                                        >  3:260043/1‑149 (MQ=255)
                                                                                   tttttAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGt                                                                <  1:456896/149‑1 (MQ=255)
                                                                                           aaGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGa                                                        >  3:440682/1‑149 (MQ=255)
                                                                                            aGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGAt                                                       >  2:376188/1‑149 (MQ=255)
                                                                                             gTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTATTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCt                                                                             <  2:527589/126‑1 (MQ=255)
                                                                                             gTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTATTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCt                                                                             >  1:527589/1‑126 (MQ=255)
                                                                                              ttGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTc                                                     <  3:739674/149‑1 (MQ=255)
                                                                                              ttGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTc                                                     >  1:240986/1‑149 (MQ=255)
                                                                                                 atatTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGt                                                  <  3:391560/149‑1 (MQ=255)
                                                                                                      aGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGga                                             >  1:897790/1‑149 (MQ=255)
                                                                                                       gATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGgag                                            >  1:201626/1‑149 (MQ=255)
                                                                                                          tGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGaaa                                         >  4:814685/1‑149 (MQ=255)
                                                                                                             tATTTTCAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAata                                      >  1:413305/1‑149 (MQ=255)
                                                                                                                 tttAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGt                                  <  4:330463/149‑1 (MQ=255)
                                                                                                                  tcaaGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATAACGTc                                 <  2:413305/147‑1 (MQ=255)
                                                                                                                    aaGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCaa                               <  2:807252/149‑1 (MQ=255)
                                                                                                                       ttACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGtt                            <  3:814685/149‑1 (MQ=255)
                                                                                                                             cTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCtt                      <  1:280354/149‑1 (MQ=255)
                                                                                                                              tCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCttt                                                                           <  3:112884/95‑1 (MQ=255)
                                                                                                                              tCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCttt                                                                           >  4:112884/1‑95 (MQ=255)
                                                                                                                              tCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTc                     >  1:707093/1‑149 (MQ=255)
                                                                                                                                acaAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAg                   >  2:78011/1‑149 (MQ=255)
                                                                                                                                acaAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAg                   >  1:510842/1‑149 (MQ=255)
                                                                                                                                acaAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAg                   >  1:192163/1‑149 (MQ=255)
                                                                                                                                acaAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAg                   >  3:502868/1‑149 (MQ=255)
                                                                                                                                  aaGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTc                 >  1:185994/1‑149 (MQ=255)
                                                                                                                                  aaGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTc                 <  4:539016/149‑1 (MQ=255)
                                                                                                                                   ataaaaaaGGCACGTCAGATGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCa                >  4:709704/3‑149 (MQ=255)
                                                                                                                                       aaaaGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAg            >  1:417599/1‑149 (MQ=255)
                                                                                                                                        aaaGGCACGTCAGATGACGTGCCTTTTTTCTTTGTCCTACCCTGTACGATTACTGTTCCCTTTCATACGTATAGCGTTTTGCTTTGTAAGCCGGCTGCATCAGATTCTGTACGGAGAAAATATCGTCACGTTCCTCTTCAGTCAACAGa           >  1:729038/1‑149 (MQ=255)
                                                                                                                                         aaGGCACGTCAGATGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGAc          >  3:705928/1‑149 (MQ=255)
                                                                                                                                          aGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGAcc         <  1:376188/149‑1 (MQ=255)
                                                                                                                                          aGGCACGTCAGATGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGAcc         >  3:563496/1‑149 (MQ=255)
                                                                                                                                            gCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACcgc       >  3:184443/1‑149 (MQ=255)
                                                                                                                                            gCACGTCAGATGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACcgc       >  2:728283/1‑149 (MQ=255)
                                                                                                                                                gTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGTTc   <  2:201626/149‑1 (MQ=255)
                                                                                                                                                 tCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGTTcc  <  2:185994/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGTTCC  >  NC_000913/4366706‑4366999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: