Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,376,291 T→C 5.3% intergenic (+26/‑26) efp → / → ecnA polyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,376,2910TC 5.3% 423.2 / 4.9 125intergenic (+26/‑26)efp/ecnApolyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B
Reads supporting (aligned to +/- strand):  ref base T (62/56);  new base C (2/5);  total (64/61)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

ACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGC  >  NC_000913/4376143‑4376439
                                                                                                                                                    |                                                                                                                                                    
aCTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCt                                                                                                                                                      <  1:936641/149‑1 (MQ=255)
  tggtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGc                                                                                                                                                    >  2:722739/1‑149 (MQ=255)
  tggtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGc                                                                                                                                                    <  4:243616/149‑1 (MQ=255)
   ggtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCa                                                                                                                                                   <  1:238055/149‑1 (MQ=255)
   ggtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCa                                                                                                                                                   <  2:520535/149‑1 (MQ=255)
    gtggCAAACCGGCTACCTTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTAAAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAc                                                                                                                                                  <  4:853458/149‑1 (MQ=255)
     tggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAcc                                                                                                                                                 <  2:720836/149‑1 (MQ=255)
       gCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCAt                                                                                                                                               >  4:693911/1‑149 (MQ=255)
       gCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCAt                                                                                                                                               >  3:274896/1‑149 (MQ=255)
         aaaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                             <  1:812582/149‑1 (MQ=255)
         aaaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                             >  3:894626/1‑149 (MQ=255)
             cGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAc                                                                                                                                                  <  3:693379/140‑1 (MQ=255)
              ggCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTAt                                                                                                                                        >  1:831621/1‑149 (MQ=255)
               gCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTAtt                                                                                                                                       <  3:367914/149‑1 (MQ=255)
                cTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTc                                                                                                                                      <  4:217909/149‑1 (MQ=255)
                 tACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCAcc                                                                                                                                                 <  3:849968/137‑1 (MQ=255)
                 tACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCAcc                                                                                                                                                 >  4:849968/1‑137 (MQ=255)
                 tACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCa                                                                                                                                     >  3:816766/1‑149 (MQ=255)
                 tACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCa                                                                                                                                     <  1:153242/149‑1 (MQ=255)
                   cccTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTa                                                                                                                                         <  1:350348/143‑1 (MQ=255)
                   cccTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTa                                                                                                                                         >  2:350348/1‑143 (MQ=255)
                    ccTGTCTACTGACGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGt                                                                                                                                  >  4:32551/1‑149 (MQ=255)
                     cTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTc                                                                                                                                 >  3:65215/1‑149 (MQ=255)
                      tGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCa                                                                                                                                >  3:613838/1‑149 (MQ=255)
                        tCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCaga                                                                                                                              >  4:725125/1‑149 (MQ=255)
                        tCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCaga                                                                                                                              <  4:65215/149‑1 (MQ=255)
                         cTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCagag                                                                                                                             <  4:266355/149‑1 (MQ=255)
                           aCTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgat                                                                                                                           <  3:291306/149‑1 (MQ=255)
                           aCTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTAAGGTCAGAgat                                                                                                                           <  3:598388/149‑1 (MQ=255)
                            cTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatg                                                                                                                          >  1:86354/1‑149 (MQ=255)
                              ggCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGAGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatgat                                                                                                                        <  1:600040/149‑1 (MQ=255)
                                     tgGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGc                                                                                                                 <  4:265197/149‑1 (MQ=255)
                                       gTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatgat                                                                                                                        >  3:428043/1‑140 (MQ=255)
                                       gTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatgat                                                                                                                        <  4:428043/140‑1 (MQ=255)
                                       gTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCt                                                                                                               >  4:376715/1‑149 (MQ=255)
                                       gTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCt                                                                                                               >  3:485434/1‑149 (MQ=255)
                                       gTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCt                                                                                                               >  2:684227/1‑149 (MQ=255)
                                       gTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCt                                                                                                               >  1:480275/1‑149 (MQ=255)
                                        ttAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCtt                                                                                                              >  1:743120/1‑149 (MQ=255)
                                        ttAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCtt                                                                                                              >  2:148668/1‑149 (MQ=255)
                                         tAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTa                                                                                                             >  2:31401/1‑149 (MQ=255)
                                          aaaGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTAt                                                                                                            >  2:10551/1‑149 (MQ=255)
                                           aaGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATc                                                                                                           <  1:148668/149‑1 (MQ=255)
                                            aGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCg                                                                                                          <  2:34832/149‑1 (MQ=255)
                                             gTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGGAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGt                                                                                                         >  1:108758/1‑149 (MQ=255)
                                              ttCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGtt                                                                                                        <  1:298878/149‑1 (MQ=255)
                                              ttCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGtt                                                                                                        <  2:629729/149‑1 (MQ=255)
                                               tCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTc                                                                                                       <  1:722739/149‑1 (MQ=255)
                                               tCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTc                                                                                                       <  2:645577/149‑1 (MQ=255)
                                                ccGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                             >  2:207815/1‑110 (MQ=255)
                                                ccGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCt                                                                                                      <  1:10551/149‑1 (MQ=255)
                                                  gCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCg                                                                                                          >  2:55406/1‑143 (MQ=255)
                                                  gCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCg                                                                                                          <  1:55406/143‑1 (MQ=255)
                                                     gTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGttt                                                                                                 >  2:533311/1‑149 (MQ=255)
                                                          tACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgctt                                                                                            >  3:639434/1‑149 (MQ=255)
                                                           aCAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttg                                                                                           >  4:321663/1‑149 (MQ=255)
                                                           aCAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttg                                                                                           >  1:529632/1‑149 (MQ=255)
                                                            cAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttgc                                                                                          >  1:475656/1‑149 (MQ=255)
                                                             aaaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGcc                                                                                         >  1:15170/1‑149 (MQ=255)
                                                             aaaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGcc                                                                                         <  1:306888/149‑1 (MQ=255)
                                                              aaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCa                                                                                        >  1:189164/1‑149 (MQ=255)
                                                                  ggCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCAc                                                                                    >  2:903655/1‑149 (MQ=255)
                                                                   gCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGTTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACg                                                                                   >  1:628294/1‑149 (MQ=255)
                                                                     gAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgct                                                                                 <  1:932075/149‑1 (MQ=255)
                                                                      aaGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctg                                                                                <  2:281612/149‑1 (MQ=255)
                                                                       aGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctgc                                                                               <  2:895677/149‑1 (MQ=255)
                                                                           atcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCAc                                                                           >  4:749275/1‑149 (MQ=255)
                                                                            tcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACg                                                                          >  4:324323/1‑149 (MQ=255)
                                                                                aGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCt                                                                      <  1:903655/149‑1 (MQ=255)
                                                                                 gTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTACAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTg                                                                     >  2:911208/1‑149 (MQ=255)
                                                                                    gATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTaa                                                                  <  4:894626/149‑1 (MQ=255)
                                                                                      tACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAaca                                                                <  3:32551/149‑1 (MQ=255)
                                                                                       aCCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAacac                                                               <  4:682119/149‑1 (MQ=255)
                                                                                         ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTAt                                                                                                                                        >  1:72081/1‑74 (MQ=255)
                                                                                         ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCg                                                             >  2:6186/1‑149 (MQ=255)
                                                                                          cGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTCGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGc                                                            >  2:867189/1‑149 (MQ=255)
                                                                                                 gTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCATCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGt                                                     <  3:693911/149‑1 (MQ=255)
                                                                                                 gTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGt                                                     <  4:602161/149‑1 (MQ=255)
                                                                                                  tGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGtt                                                    >  1:121033/1‑149 (MQ=255)
                                                                                                       aCGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGc                                               <  2:181889/149‑1 (MQ=255)
                                                                                                         gTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGc                                                                                                                 >  1:316541/1‑81 (MQ=255)
                                                                                                         gTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGa                                             >  3:9251/1‑149 (MQ=255)
                                                                                                         gTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGa                                             >  1:516534/1‑149 (MQ=255)
                                                                                                          tctctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGaa                                            >  1:562286/1‑149 (MQ=255)
                                                                                                                gcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTc                                                                                                       <  1:768608/84‑1 (MQ=255)
                                                                                                                gcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTc                                                                                                       >  2:768608/1‑84 (MQ=255)
                                                                                                                   tGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCaaa                                   <  4:322713/149‑1 (MQ=255)
                                                                                                                   tGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCaaa                                   <  4:613838/149‑1 (MQ=255)
                                                                                                                     aaGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTc                                                  >  1:100442/1‑132 (MQ=255)
                                                                                                                        tAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgc                                                                                              <  2:413075/85‑1 (MQ=255)
                                                                                                                        tAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACAtct                              >  1:32650/1‑149 (MQ=255)
                                                                                                                          aTGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCg                            <  4:69637/149‑1 (MQ=255)
                                                                                                                          aTGCGGCTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCg                            <  3:121229/149‑1 (MQ=255)
                                                                                                                           tGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATTATGAAACGCCTTATCGTTCTTGTTTTGCTTGcc                                                                                         <  2:286282/87‑1 (MQ=255)
                                                                                                                           tGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGcc                                                                                         >  1:286282/1‑87 (MQ=255)
                                                                                                                           tGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGcc                                                                                         <  2:825677/87‑1 (MQ=255)
                                                                                                                           tGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGcc                                                                                         >  1:825677/1‑87 (MQ=255)
                                                                                                                           tGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCgg                           <  3:803838/149‑1 (MQ=255)
                                                                                                                               gTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAAc                       >  4:467821/1‑149 (MQ=255)
                                                                                                                                      tgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTAGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCAtca                >  2:536534/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGCCAGC  >  NC_000913/4376143‑4376439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: