Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,376,633 C→T 5.5% G27G (GGC→GGT ecnB → entericidin B membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,376,6330CT 5.5% 421.1 / 9.1 126G27G (GGC→GGTecnBentericidin B membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (63/56);  new base T (5/2);  total (68/58)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 126 total aligned reads displayed.

GGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGC  >  NC_000913/4376486‑4376779
                                                                                                                                                   |                                                                                                                                                  
ggCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCg                                                                                                                                                   >  3:696942/1‑149 (MQ=255)
  cGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGtt                                                                                                                                                 <  2:562286/149‑1 (MQ=255)
    aTGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGTCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTgg                                                                                                                                               <  2:285554/149‑1 (MQ=255)
     tgttgtCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGt                                                                                                                                              <  2:68376/149‑1 (MQ=255)
     tgttgtCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGt                                                                                                                                              <  2:587994/149‑1 (MQ=255)
        tgtCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGaa                                                                                                                                           <  2:886827/149‑1 (MQ=255)
        tgtCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGaa                                                                                                                                           <  4:396545/149‑1 (MQ=255)
        tgtCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGaa                                                                                                                                           <  1:521318/149‑1 (MQ=255)
        tgtCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGaa                                                                                                                                           <  2:370067/149‑1 (MQ=255)
          tCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGa                                                                                                                                         >  1:651040/1‑149 (MQ=255)
              ttattaATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAtt                                                                                                                                     <  3:899660/149‑1 (MQ=255)
                   aaTTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGa                                                                                                                                <  2:189164/149‑1 (MQ=255)
                   aaTTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGa                                                                                                                                <  2:125645/149‑1 (MQ=255)
                    aTTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGAt                                                                                                                               <  2:441845/149‑1 (MQ=255)
                    aTTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGAt                                                                                                                               <  2:649576/149‑1 (MQ=255)
                          tataGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGt                                                                                                                         >  3:373903/1‑149 (MQ=255)
                          tataGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGt                                                                                                                         >  1:593380/1‑149 (MQ=255)
                             aGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAAc                                                                                                                      <  4:373903/149‑1 (MQ=255)
                                 aaaCATAAATGGCATTACCTAAAAGGCAGACGTTATGGTGAAGGAGACAATTGCAGCGATCTTTTCTGTTCTGGGGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGAGGGCGGTAACGCGa                                                                                                                  >  2:830503/1‑149 (MQ=255)
                                 aaaCATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGa                                                                                                                  >  4:149565/1‑149 (MQ=255)
                                  aaCATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGAt                                                                                                                 <  1:129225/149‑1 (MQ=255)
                                      taaataaCATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCt                                                                                                             >  1:375262/1‑149 (MQ=255)
                                        aataaCATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTgg                                                                                                           >  3:662000/1‑149 (MQ=255)
                                          taaCATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGgcg                                                                                                         >  1:819893/1‑149 (MQ=255)
                                          taaCATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGgcg                                                                                                         >  1:577570/1‑149 (MQ=255)
                                             cATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCgcag                                                                                                      >  2:889127/1‑149 (MQ=255)
                                             cATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCgcag                                                                                                      <  2:835792/149‑1 (MQ=255)
                                               ttACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCgcagca                                                                                                    >  2:141788/1‑149 (MQ=255)
                                                 aCCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAAc                                                                                                  <  1:821092/149‑1 (MQ=255)
                                                    tAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGaa                                                                                               >  4:610157/1‑149 (MQ=255)
                                                     aaaaGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGaaa                                                                                              >  4:502743/1‑149 (MQ=255)
                                                      aaaGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAg                                                                                             >  1:227119/1‑149 (MQ=255)
                                                      aaaGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGAGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAg                                                                                             >  4:425504/1‑149 (MQ=255)
                                                       aaGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAgc                                                                                            >  4:486224/1‑149 (MQ=255)
                                                         ggAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAgcgc                                                                                          <  4:687934/149‑1 (MQ=255)
                                                          gAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCgca                                                                                         <  3:262324/149‑1 (MQ=255)
                                                                 ttATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCagcaataa                                                                                  >  1:796600/1‑149 (MQ=255)
                                                                 ttATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAACCGCagcaataa                                                                                  <  3:598520/149‑1 (MQ=255)
                                                                        gaagaaGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAgcaataagcaataa                                                                           <  3:486224/149‑1 (MQ=255)
                                                                        gaagaaGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAgcaataagcaataa                                                                           <  2:444324/149‑1 (MQ=255)
                                                                        gaagaaGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGAGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAgcaataagcaataa                                                                           <  4:664597/149‑1 (MQ=255)
                                                                         aagaagACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAAc                                                                          >  2:902322/1‑149 (MQ=255)
                                                                           gaagaCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACgg                                                                        >  3:417149/1‑149 (MQ=255)
                                                                           gaagaCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACgg                                                                        <  3:253179/149‑1 (MQ=255)
                                                                           gaagaCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACgg                                                                        >  3:721813/1‑149 (MQ=255)
                                                                            aagaCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGt                                                                       >  4:243740/1‑149 (MQ=255)
                                                                            aagaCAATTGCAGCGATCCTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGt                                                                       >  4:353125/1‑149 (MQ=255)
                                                                              gaCAATTGCAGCGGTCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTAc                                                                     >  2:99199/1‑149 (MQ=255)
                                                                              gaCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAgcg                                                                                           <  1:244760/127‑1 (MQ=255)
                                                                              gaCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAgcg                                                                                           >  2:244760/1‑127 (MQ=255)
                                                                               aCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAt                                                                                                                                      <  2:807548/83‑1 (MQ=255)
                                                                               aCAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAt                                                                                                                                      >  1:807548/1‑83 (MQ=255)
                                                                                 aaTTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGAc                                                                  >  4:479897/1‑149 (MQ=255)
                                                                                   ttGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAg                                                                <  1:361964/149‑1 (MQ=255)
                                                                                   ttGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAg                                                                <  2:227119/149‑1 (MQ=255)
                                                                                      cAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCtg                                                             >  2:695267/1‑149 (MQ=255)
                                                                                       aGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGAtt                                                                                                                <  1:292953/97‑1 (MQ=255)
                                                                                       aGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGAtt                                                                                                                >  2:292953/1‑97 (MQ=255)
                                                                                       aGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGAt                                                                                                                 <  3:823935/96‑1 (MQ=255)
                                                                                       aGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGAt                                                                                                                 >  4:823935/1‑96 (MQ=255)
                                                                                       aGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCtgt                                                            <  3:857646/149‑1 (MQ=255)
                                                                                              ttttCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGc                                                     >  4:100401/1‑149 (MQ=255)
                                                                                              ttttCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGc                                                     >  4:764108/1‑149 (MQ=255)
                                                                                              ttttCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGc                                                     >  4:386615/1‑149 (MQ=255)
                                                                                                          gTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGc                                                                                                                            >  1:225656/1‑66 (MQ=255)
                                                                                                          gTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGtttttt                                         <  1:286076/149‑1 (MQ=255)
                                                                                                             cTTTCAACAGTATTAACTGCCTGCAACACCACGAGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTg                                      <  3:425504/149‑1 (MQ=255)
                                                                                                              tttCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGc                                     <  2:651040/149‑1 (MQ=255)
                                                                                                              tttCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTAGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTACCGTTTGTTTTTTCTGc                                     >  2:675334/1‑149 (MQ=255)
                                                                                                                  aaCAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATa                                 >  1:344650/1‑149 (MQ=255)
                                                                                                                       tATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTcac                            >  1:362770/1‑149 (MQ=255)
                                                                                                                         ttAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACaa                          >  3:123930/1‑149 (MQ=255)
                                                                                                                          tAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACaaa                         <  1:203760/149‑1 (MQ=255)
                                                                                                                           aaCTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGCTTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAg                        >  1:53642/1‑149 (MQ=255)
                                                                                                                            aCTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAgg                       <  2:819893/149‑1 (MQ=255)
                                                                                                                             ctgcctgcAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGt                      >  1:288743/1‑149 (MQ=255)
                                                                                                                                 ctgcAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATa                  >  3:124321/1‑149 (MQ=255)
                                                                                                                                 ctgcAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATa                  <  3:610157/149‑1 (MQ=255)
                                                                                                                                 ctgcAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGCTAATa                  <  1:75750/149‑1 (MQ=255)
                                                                                                                                  tgcAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTCTGCGATAGTCACAAAGGTAATAGt                >  2:841247/1‑149 (MQ=255)
                                                                                                                                  accAACGCCACGCGTGGTGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCAGGCGCAGCAACGAAAGCTCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTCGTTTTTTCTCCGATAGACACAAGGGTAATAg                 >  3:572324/3‑149 (MQ=255)
                                                                                                                                   ccAACGCCACGCGTGGTGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGt                >  2:9927/2‑149 (MQ=255)
                                                                                                                                    cAACGCCACGCGTGGTGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGtt               >  3:257567/1‑149 (MQ=255)
                                                                                                                                    cAACGCCACGCGTGGTGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGtt               >  1:883954/1‑149 (MQ=255)
                                                                                                                                    cAACGCCACGCGTGGTGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGtt               >  1:857404/1‑149 (MQ=255)
                                                                                                                                    cAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGtt               <  4:696942/149‑1 (MQ=255)
                                                                                                                                    cAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGtt               >  3:705111/1‑149 (MQ=255)
                                                                                                                                     aaCACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTg              >  4:311378/1‑149 (MQ=255)
                                                                                                                                      acgccacGCGTGGTGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGa             <  2:883954/146‑1 (MQ=255)
                                                                                                                                      acgccacGCGTGGTGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGa             <  2:857404/146‑1 (MQ=255)
                                                                                                                                      acacTACGCGTGGCGTTGGTGAAGTCATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGa             >  1:794698/1‑149 (MQ=255)
                                                                                                                                        accacGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATa                  <  3:748380/142‑1 (MQ=255)
                                                                                                                                        accacGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATa                  >  4:748380/1‑142 (MQ=255)
                                                                                                                                        accacGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGaaa           <  3:502743/149‑1 (MQ=255)
                                                                                                                                        accacGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGaaa           <  2:128561/149‑1 (MQ=255)
                                                                                                                                        accacGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGaaa           <  2:569203/149‑1 (MQ=255)
                                                                                                                                             gcgTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCtgt                                                            >  1:878441/1‑95 (MQ=255)
                                                                                                                                             gcgTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTccc      >  2:778787/1‑149 (MQ=255)
                                                                                                                                              cgTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTcccc     <  1:830503/149‑1 (MQ=255)
                                                                                                                                                 ggCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGc  <  2:234671/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGAAAGCGCAGCAATAAGCAATAACGGTACGACAGCTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGC  >  NC_000913/4376486‑4376779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: