Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,410,026 G→A 6.4% intergenic (+20/‑107) rlmB → / → yjfI 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,410,0260GA 6.4% 247.6 / 5.7 78intergenic (+20/‑107)rlmB/yjfI23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein
Reads supporting (aligned to +/- strand):  ref base G (33/40);  new base A (3/2);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

ATGCGTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAA  >  NC_000913/4409878‑4410172
                                                                                                                                                    |                                                                                                                                                   
aTGCGTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAg                                                                                                                                                     >  2:658844/1‑149 (MQ=255)
    gTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCAt                                                                                                                                                 <  3:34887/149‑1 (MQ=255)
    gTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCAt                                                                                                                                                 <  4:637239/149‑1 (MQ=255)
     tCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATc                                                                                                                                                >  1:525869/1‑149 (MQ=255)
     tCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATc                                                                                                                                                <  4:563959/149‑1 (MQ=255)
       gCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATGTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCa                                                                                                                                              >  3:640698/1‑149 (MQ=255)
          tGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGAt                                                                                                                                           <  4:500075/149‑1 (MQ=255)
             cTCGCGAACATTGCGATGAGTTGATCAGCATCCCGATGGTTGGAAGCCTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCCGTGCGCCAGCGCAGCTAATTTCTCAGAAGTATGAAAAGCCATCCAGATTTg                                                                                                                                        <  4:576242/149‑1 (MQ=255)
              tCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTgg                                                                                                                                       >  1:393389/1‑149 (MQ=255)
               cGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGa                                                                                                                                      <  3:357494/149‑1 (MQ=255)
                gTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGAt                                                                                                                                     >  3:73073/1‑149 (MQ=255)
                gTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGAt                                                                                                                                     >  3:590886/1‑149 (MQ=255)
                 tGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATg                                                                                                                                    >  2:182114/1‑149 (MQ=255)
                   aaCATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑t                                                                                                                                 <  3:150145/149‑1 (MQ=255)
                     cATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑ttt                                                                                                                               >  1:666062/1‑149 (MQ=255)
                         gCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑ttttttt                                                                                                                           <  2:466479/149‑1 (MQ=255)
                         gCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑ttttttt                                                                                                                           <  4:54209/149‑1 (MQ=255)
                          cGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑tttttttt                                                                                                                          <  3:650023/149‑1 (MQ=255)
                           gATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGa                                                                                                                                      >  4:20535/1‑137 (MQ=255)
                           gATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGa                                                                                                                                      <  3:20535/137‑1 (MQ=255)
                           gATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑ttttttttt                                                                                                                         <  3:371475/149‑1 (MQ=255)
                           gATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑ttttttttt                                                                                                                         <  2:349268/149‑1 (MQ=255)
                                 ttGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑tttttttttttttta                                                                                                                   >  4:137555/1‑143 (MQ=255)
                                    aTCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGc                                                                                                                >  2:697372/1‑149 (MQ=255)
                                     tCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCt                                                                                                               <  1:182114/149‑1 (MQ=255)
                                        gCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGt                                                                                                            >  3:331125/1‑149 (MQ=255)
                                         cATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTa                                                                                                           <  2:125662/149‑1 (MQ=255)
                                          aTCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTaa                                                                                                          <  3:509794/149‑1 (MQ=255)
                                             ccGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGAt                                                                                                       >  2:746243/1‑149 (MQ=255)
                                                aTGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAAt                                                                                                    <  1:85697/149‑1 (MQ=255)
                                                   gttGGAAGCGTTTCTTCCCTGAACGTTTCGTTTGCAACGGGAATTTGTTTATTTGAAGGGGTGCGCCAGCGCAGTTAATTTCTCAAAATAATAAAAAGCCATCCAGATTGGGTTGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTAc                                                                                                 <  4:283356/147‑1 (MQ=255)
                                                     tGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGt                                                                                               >  2:729382/1‑149 (MQ=255)
                                                     tGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGt                                                                                               >  3:889265/1‑149 (MQ=255)
                                                      ggAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTa                                                                                              >  2:555869/1‑149 (MQ=255)
                                                      ggAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTa                                                                                              <  4:73073/149‑1 (MQ=255)
                                                            gTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCaa                                                                                        >  4:137034/1‑149 (MQ=255)
                                                             tttCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCaaa                                                                                       >  4:698098/1‑149 (MQ=255)
                                                                   cccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCa                                                                                 <  2:525869/149‑1 (MQ=255)
                                                                    ccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCTATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAt                                                                                <  4:640698/149‑1 (MQ=255)
                                                                     cTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtt                                                                               >  1:145725/1‑149 (MQ=255)
                                                                     cTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGG‑cttttcat                                                                                                                          <  3:786095/106‑4 (MQ=38)
                                                                     cTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGG‑cttttcat                                                                                                                          >  4:786095/1‑103 (MQ=38)
                                                                      tGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAttt                                                                              <  1:160305/149‑1 (MQ=255)
                                                                        aaCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCATCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGAATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTtta                                                                            <  2:461099/149‑1 (MQ=255)
                                                                        aaCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTtta                                                                            <  1:746243/149‑1 (MQ=255)
                                                                        aaCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTtta                                                                            <  3:491656/149‑1 (MQ=255)
                                                                              tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAata                                                                      <  2:393389/149‑1 (MQ=255)
                                                                               cGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAatat                                                                     <  1:658844/149‑1 (MQ=255)
                                                                                ggTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAatat                                                                     <  4:590886/149‑1 (MQ=255)
                                                                                  ttGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTa                                                                  >  2:554131/1‑149 (MQ=255)
                                                                                   tGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaa                                                                 <  3:137034/149‑1 (MQ=255)
                                                                                     cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAAc                                                               <  3:698098/149‑1 (MQ=255)
                                                                                      gACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGG‑cttt                                                                                                                              <  4:891618/85‑1 (MQ=38)
                                                                                      gACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGG‑cttt                                                                                                                              >  3:891618/1‑85 (MQ=38)
                                                                                        ccGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTa                                                            >  3:17967/1‑149 (MQ=255)
                                                                                              tttGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                                                      >  1:295363/1‑149 (MQ=255)
                                                                                                tGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTatat                                                    <  1:729382/149‑1 (MQ=255)
                                                                                                   ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGa                                                 >  3:251144/1‑149 (MQ=255)
                                                                                                     aTTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaat                                               >  2:177393/1‑149 (MQ=255)
                                                                                                      tttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAttt                                                                              >  4:866215/1‑117 (MQ=255)
                                                                                                      tttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAttt                                                                              <  3:866215/117‑1 (MQ=255)
                                                                                                        tGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaataat                                            <  1:555869/149‑1 (MQ=255)
                                                                                                          aaGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTatat                                                    >  1:389048/1‑139 (MQ=255)
                                                                                                          aaGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTatat                                                    <  2:389048/139‑1 (MQ=255)
                                                                                                                   gcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATgg                                 >  2:131203/1‑149 (MQ=255)
                                                                                                                     tggcgcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGt                                                                                                                       <  1:630426/59‑1 (MQ=255)
                                                                                                                     tggcgcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGt                                                                                                                       >  2:630426/3‑61 (MQ=255)
                                                                                                                           aGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGtt                         <  4:331125/149‑1 (MQ=255)
                                                                                                                           aGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGtt                         <  2:295363/149‑1 (MQ=255)
                                                                                                                             cTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTgg                       >  2:333353/1‑149 (MQ=255)
                                                                                                                                 tttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCt                   >  3:164362/1‑149 (MQ=255)
                                                                                                                                 tttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGAAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCt                   <  4:889265/149‑1 (MQ=255)
                                                                                                                                  ttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTa                  <  3:721150/149‑1 (MQ=255)
                                                                                                                                     tcAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCg               >  4:654237/1‑149 (MQ=255)
                                                                                                                                     tcAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCg               >  1:122884/1‑149 (MQ=255)
                                                                                                                                               gAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGc     >  4:438135/1‑149 (MQ=255)
                                                                                                                                               gAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGc     >  4:926366/1‑149 (MQ=255)
                                                                                                                                               aaaaaACCATCCAAATCTGGATGGCTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTg      >  1:710928/2‑149 (MQ=255)
                                                                                                                                                  aaGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCaaa  <  3:438135/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATGCGTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAA  >  NC_000913/4409878‑4410172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑