Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,414,533 G→T 77.9% A87S (GCC→TCC)  aidB → DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,414,5330GT77.9% 227.6 / 82.4 114A87S (GCC→TCC) aidBDNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (9/16);  new base T (41/47);  total (50/64)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

Only 100 of 115 total aligned reads displayed.

GGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGAT  >  NC_000913/4414388‑4414678
                                                                                                                                                 |                                                                                                                                                 
ggATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCcctcct                                                                                                                                                <  2:30526/149‑1 (MQ=255)
  aTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTgg                                                                                                                                              >  2:191532/1‑149 (MQ=255)
       gATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACct                                                                                                                                         <  1:249267/149‑1 (MQ=255)
         ttttCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACctgc                                                                                                                                       <  3:777831/149‑1 (MQ=255)
           ttCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACctgctg                                                                                                                                     <  1:759181/149‑1 (MQ=255)
            tCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGa                                                                                                                                    <  3:3339/149‑1 (MQ=255)
            tCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGa                                                                                                                                    <  2:482502/149‑1 (MQ=255)
             cTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGAt                                                                                                                                   <  4:874511/149‑1 (MQ=255)
             cTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGAt                                                                                                                                   <  3:141307/149‑1 (MQ=255)
              tAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATg                                                                                                                                  <  1:818936/149‑1 (MQ=255)
              tAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATg                                                                                                                                  >  1:707671/1‑149 (MQ=255)
               aGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGc                                                                                                                                 <  2:612329/149‑1 (MQ=255)
               aGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGc                                                                                                                                 <  2:578929/149‑1 (MQ=255)
                gCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCACGGACGCCGTCTGGACGATGCGCGTTTTCACTCCCCCTGCCACgtgctgccgca                                                                                                                                <  2:846184/149‑6 (MQ=255)
                 ccAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTATGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGccg                                                                                                                               >  4:400582/1‑147 (MQ=255)
                  cAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAgg                                                                                                                              >  3:63278/1‑149 (MQ=255)
                  cAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGAGGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAgg                                                                                                                              <  2:67394/149‑1 (MQ=255)
                   aGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGgc                                                                                                                             <  1:928321/149‑1 (MQ=255)
                    gTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGcc                                                                                                                                                 <  4:137100/128‑1 (MQ=255)
                       ttGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTa                                                                                                                         <  4:562054/149‑1 (MQ=255)
                        tGGTCAGCAGTTAGGAACGGATGAATCCCTTGAACTGGGGCGGCTAGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTAt                                                                                                                        <  3:760387/149‑1 (MQ=255)
                          gTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGt                                                                                                                      >  2:279015/1‑149 (MQ=255)
                           tCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTa                                                                                                                     >  4:113349/1‑149 (MQ=255)
                           tCAGCAGTTAGGAACGGCTGAAGCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCTTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACGTGCTGATGCAGGCGGTATGTa                                                                                                                     >  4:251799/1‑149 (MQ=255)
                            cagcagTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTAc                                                                                                                    <  3:88883/149‑1 (MQ=255)
                               cagTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCaa                                                                                                                 >  1:717508/1‑149 (MQ=255)
                                agTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAAt                                                                                                                >  4:305774/1‑149 (MQ=255)
                                   tAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCgg                                                                                                             <  1:929558/149‑1 (MQ=255)
                                   tAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCgg                                                                                                             <  4:377423/149‑1 (MQ=255)
                                    aGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCggg                                                                                                            <  2:640668/149‑1 (MQ=255)
                                     ggAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGt                                                                                                           <  3:810368/149‑1 (MQ=255)
                                      gAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGt                                                                                                                      <  2:204735/137‑1 (MQ=255)
                                      gAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTg                                                                                                          >  1:413994/1‑149 (MQ=255)
                                       aaCGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGc                                                                                                         <  2:413994/149‑1 (MQ=255)
                                            cTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAAt                                                                                                    >  1:174446/1‑149 (MQ=255)
                                             tGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATc                                                                                                   >  4:227365/1‑149 (MQ=255)
                                                aTCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTg                                                                                                >  4:384593/1‑149 (MQ=255)
                                                   ccTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCt                                                                                             >  3:314790/1‑149 (MQ=255)
                                                   ccTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCt                                                                                             >  1:167949/1‑149 (MQ=255)
                                                    cTTGAACTGGGGCGGCTGGCGGATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACctgc                                                                                                                                       >  3:118796/1‑106 (MQ=255)
                                                    cTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTg                                                                                            >  2:533997/1‑149 (MQ=255)
                                                     ttGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTgg                                                                                           >  1:251809/1‑149 (MQ=255)
                                                      tGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTggg                                                                                          <  2:707671/149‑1 (MQ=255)
                                                      tGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTggg                                                                                          <  3:384593/149‑1 (MQ=255)
                                                      tGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACACCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTggg                                                                                          <  2:829868/149‑1 (MQ=255)
                                                       gAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGga                                                                                         >  3:425454/1‑149 (MQ=255)
                                                           tGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGcc                                                                                              <  4:168798/140‑1 (MQ=255)
                                                           tGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGcc                                                                                              >  3:168798/1‑140 (MQ=255)
                                                            ggggCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGaagaag                                                                                    <  2:717508/149‑1 (MQ=255)
                                                             gggCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAagaaga                                                                                   >  3:606505/1‑149 (MQ=255)
                                                                   cTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGAcgctcg                                                                             >  4:52270/1‑149 (MQ=255)
                                                                        gAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCg                                                                        >  3:441014/1‑149 (MQ=255)
                                                                        gAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCg                                                                        >  3:699481/1‑149 (MQ=255)
                                                                          aTGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGgc                                                                      <  2:167949/149‑1 (MQ=255)
                                                                            gtgAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGgcgc                                                                    >  1:545184/1‑149 (MQ=255)
                                                                                 tCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTtg                                                               >  3:187963/1‑149 (MQ=255)
                                                                                    gCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTgg                                                            >  1:225502/1‑149 (MQ=255)
                                                                                    gCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTgg                                                            >  4:240963/1‑149 (MQ=255)
                                                                                      cTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGgcg                                                          >  2:536642/1‑149 (MQ=255)
                                                                                       tGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTgg                                                                                           >  1:12013/1‑115 (MQ=255)
                                                                                       tGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTgg                                                                                           <  2:12013/115‑1 (MQ=255)
                                                                                       tGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGgcgc                                                         >  2:492096/1‑149 (MQ=255)
                                                                                       tGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGgcgc                                                         <  1:259306/149‑1 (MQ=255)
                                                                                         aaTTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATc                                                                                                               >  3:134906/1‑93 (MQ=255)
                                                                                         aaTTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATc                                                                                                               <  4:134906/93‑1 (MQ=255)
                                                                                           ttattGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGgcgcgcgc                                                     >  1:89545/1‑149 (MQ=255)
                                                                                                  gcTACGATGCGTAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGt                                              >  3:540085/1‑149 (MQ=255)
                                                                                                   cTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGtt                                             >  3:458970/1‑149 (MQ=255)
                                                                                                    tACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGttt                                            >  2:440333/1‑149 (MQ=255)
                                                                                                     aCGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGtttt                                           <  3:198599/149‑1 (MQ=255)
                                                                                                       gATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTAt                                         >  1:408459/1‑149 (MQ=255)
                                                                                                       gATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTAt                                         <  1:184245/149‑1 (MQ=255)
                                                                                                         tGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGt                                       <  4:63278/149‑1 (MQ=255)
                                                                                                         tGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGt                                       <  2:850205/149‑1 (MQ=255)
                                                                                                           cgcAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTa                                     <  3:669800/149‑1 (MQ=255)
                                                                                                           cgcAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTa                                     <  2:649297/149‑1 (MQ=255)
                                                                                                           cgcAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTa                                     <  1:533997/149‑1 (MQ=255)
                                                                                                                        tCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTAt                                         <  4:291434/132‑1 (MQ=255)
                                                                                                                        tCTGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTAt                                         >  3:291434/1‑132 (MQ=255)
                                                                                                                          tGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAg                      >  1:145098/1‑149 (MQ=255)
                                                                                                                          tGGACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAg                      <  2:145098/149‑1 (MQ=255)
                                                                                                                          tGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAg                      <  4:210228/149‑1 (MQ=255)
                                                                                                                            gACGATGTGCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGc                    >  1:412385/1‑149 (MQ=255)
                                                                                                                                  gtgCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTc              <  1:536642/149‑1 (MQ=255)
                                                                                                                                    gCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCgcgcgg                                                   <  2:454958/110‑1 (MQ=255)
                                                                                                                                    gCGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCgcgcgg                                                   >  1:454958/1‑110 (MQ=255)
                                                                                                                                     cGTTTTCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGtt           <  2:173238/149‑1 (MQ=255)
                                                                                                                                     cGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGtt           <  3:240963/149‑1 (MQ=255)
                                                                                                                                       ttttCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTAt         <  4:314790/149‑1 (MQ=255)
                                                                                                                                       ttttCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTAt         >  4:201575/1‑149 (MQ=255)
                                                                                                                                         ttCACCCCTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGt       <  1:55914/149‑1 (MQ=255)
                                                                                                                                          tCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTc      <  3:595340/149‑1 (MQ=255)
                                                                                                                                          tCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTc      <  3:713390/149‑1 (MQ=255)
                                                                                                                                             ccccTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGa   <  1:191532/149‑1 (MQ=255)
                                                                                                                                             ccccTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGa   <  2:408459/149‑1 (MQ=255)
                                                                                                                                             ccccTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGa   <  4:699481/149‑1 (MQ=255)
                                                                                                                                              cccTCCTGGCACCTGCTGATGCAGGCGGTATGTACCAATCGGGTGCACAATCTTGCCTAGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGACGCGTTTTATGTTACATGCGCAGGATGAGGCAGGGTCGTTATGTCCGAt  <  3:251799/149‑1 (MQ=255)
                                                                                                                                              cccTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGtt                                             >  1:144693/1‑106 (MQ=255)
                                                                                                                                              cccTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGt            >  3:840781/1‑139 (MQ=255)
                                                                                                                                              cccTCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGt            <  4:840781/139‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGAT  >  NC_000913/4414388‑4414678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: