Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,424,468 A→C 7.8% intergenic (+82/+48) ulaF → / ← yjfY L‑ribulose 5‑phosphate 4‑epimerase/YhcN family protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,424,4680AC 7.8% 340.7 / 18.5 103intergenic (+82/+48)ulaF/yjfYL‑ribulose 5‑phosphate 4‑epimerase/YhcN family protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (48/47);  new base C (2/6);  total (50/53)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 105 total aligned reads displayed.

AGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTT  >  NC_000913/4424321‑4424613
                                                                                                                                                    |                                                                                                                                                 
aGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGaa                                                                                                                                                  >  3:773181/1‑149 (MQ=255)
aGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGaa                                                                                                                                                  >  4:241387/1‑149 (MQ=255)
   ttCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggg                                                                                                                                               >  3:687146/1‑149 (MQ=255)
   ttCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggg                                                                                                                                               <  1:184427/149‑1 (MQ=255)
   ttCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggg                                                                                                                                               <  2:533575/149‑1 (MQ=255)
    tCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAAGCCCCCTGCGAAggg                                                                                                                                               <  3:517084/149‑1 (MQ=255)
    tCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAgggg                                                                                                                                              <  4:227035/149‑1 (MQ=255)
         atgaATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgcttt                                                                                                                                         <  1:272013/149‑4 (MQ=255)
          tgaATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgctttt                                                                                                                                        <  2:25077/149‑5 (MQ=255)
           gaATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgcttttt                                                                                                                                       <  1:416794/149‑6 (MQ=255)
            aaTAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgctttttt                                                                                                                                      <  3:271513/149‑7 (MQ=255)
            aaTAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagca                                                                                                                                      >  2:86680/1‑149 (MQ=255)
            aaTAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagca                                                                                                                                      >  3:239634/1‑149 (MQ=255)
             aTAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagcaa                                                                                                                                     >  2:92028/1‑149 (MQ=255)
              tAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgctt                                                                                                                                          >  4:731747/1‑141 (MQ=255)
              tAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgctt                                                                                                                                          <  3:731747/143‑3 (MQ=255)
              tAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagcaaa                                                                                                                                    <  2:411244/149‑1 (MQ=255)
              tAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagcaaa                                                                                                                                    <  4:677402/149‑1 (MQ=255)
              tAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAAc                                                                                                                                   <  1:478232/149‑1 (MQ=255)
               aaaCACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgcttttt                                                                                                                                       <  4:405917/145‑6 (MQ=255)
               aaaCACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgcttttt                                                                                                                                       >  3:405917/1‑140 (MQ=255)
               aaaCACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAAc                                                                                                                                   <  3:105933/149‑1 (MQ=255)
                aaCACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACt                                                                                                                                  >  1:262234/1‑149 (MQ=255)
                 acacTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTa                                                                                                                                 >  1:494040/1‑149 (MQ=255)
                   acTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATg                                                                                                                               <  1:298819/149‑1 (MQ=255)
                   acTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATg                                                                                                                               >  2:888533/1‑149 (MQ=255)
                            gTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGttt                                                                                                                      >  3:233315/1‑149 (MQ=255)
                              aaaCACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                                                                                                                    <  4:773181/149‑1 (MQ=255)
                               aaCACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGt                                                                                                                   >  1:792659/1‑149 (MQ=255)
                                acacGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGtt                                                                                                                  <  1:33221/149‑1 (MQ=255)
                                acacGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGtt                                                                                                                  <  4:687146/149‑1 (MQ=255)
                                 cacGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTg                                                                                                                 >  4:512242/1‑149 (MQ=255)
                                  acGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTgg                                                                                                                >  3:900567/1‑149 (MQ=255)
                                  acGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTgg                                                                                                                <  4:239634/149‑1 (MQ=255)
                                   cGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGt                                                                                                               <  3:296655/149‑1 (MQ=255)
                                   cGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAAAGTTTCGTTGGt                                                                                                               <  3:596799/149‑1 (MQ=255)
                                    ggTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGCAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGtt                                                                                                              <  3:790011/149‑1 (MQ=255)
                                    ggTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGtt                                                                                                              <  3:241387/149‑1 (MQ=255)
                                         tAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATAcc                                                                                                         >  4:828782/1‑149 (MQ=255)
                                         tAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATAcc                                                                                                         >  1:436424/1‑149 (MQ=255)
                                             gCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGt                                                                                                     >  2:554658/1‑149 (MQ=255)
                                             gCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGt                                                                                                     >  2:318732/1‑149 (MQ=255)
                                             gCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGt                                                                                                     >  2:172955/1‑149 (MQ=255)
                                             gCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGt                                                                                                     >  1:584490/1‑149 (MQ=255)
                                             gCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTGTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGt                                                                                                     >  3:243988/1‑149 (MQ=255)
                                                 attaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTa                                                                                                 <  4:485230/149‑1 (MQ=255)
                                                 attaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTa                                                                                                 <  3:744387/149‑1 (MQ=255)
                                                 attaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTa                                                                                                 <  1:188727/149‑1 (MQ=255)
                                                  ttaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAg                                                                                                <  3:719014/149‑1 (MQ=255)
                                                  ttaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAg                                                                                                <  2:262234/149‑1 (MQ=255)
                                                       ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATa                                                                                           >  2:60149/1‑149 (MQ=255)
                                                       ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATa                                                                                           >  2:263324/1‑149 (MQ=255)
                                                            aaGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAatat                                                                                      <  1:147723/149‑1 (MQ=255)
                                                              gTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATcc                                                                                    <  1:92028/149‑1 (MQ=255)
                                                              gTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATcc                                                                                    >  4:361201/1‑149 (MQ=255)
                                                                aGAACGCGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgc                                                                                  <  4:620812/149‑1 (MQ=255)
                                                                   acacGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCTGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCt                                                                               >  4:234792/1‑149 (MQ=255)
                                                                   acacGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCt                                                                               >  4:819/1‑149 (MQ=255)
                                                                     acGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTgg                                                                             >  4:823840/1‑149 (MQ=255)
                                                                         gcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTg                                                                         >  3:249743/1‑149 (MQ=255)
                                                                               gAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGt                                                                                                     >  4:8/1‑115 (MQ=255)
                                                                               gAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGt                                                                                                     <  3:8/115‑1 (MQ=255)
                                                                               gAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATAtt                                                                   >  2:542268/1‑149 (MQ=255)
                                                                               gAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATAtt                                                                   <  3:828782/149‑1 (MQ=255)
                                                                                aaaTTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTg                                                                  <  2:178819/149‑1 (MQ=255)
                                                                                   tttCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCg                                                               <  1:888533/149‑1 (MQ=255)
                                                                                   tttCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCg                                                               <  4:438744/149‑1 (MQ=255)
                                                                                    ttccttccTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGt                                                                                                     <  1:757738/110‑1 (MQ=255)
                                                                                    ttccttccTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGt                                                                                                     >  2:757738/1‑110 (MQ=255)
                                                                                    ttccttccTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGt                                                              <  3:177848/149‑1 (MQ=255)
                                                                                        ttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACt                                                          >  4:83211/1‑149 (MQ=255)
                                                                                               ggAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGt                                                   >  2:24418/1‑149 (MQ=255)
                                                                                                 agaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTc                                                 <  1:554658/149‑1 (MQ=255)
                                                                                                    tAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCg                                              >  4:291939/1‑149 (MQ=255)
                                                                                                        tGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATc                                          >  4:913748/1‑149 (MQ=255)
                                                                                                        tGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATc                                          <  3:447963/149‑1 (MQ=255)
                                                                                                          gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGttt                                                                                                                      >  2:552191/1‑71 (MQ=255)
                                                                                                          gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGttt                                                                                                                      <  1:552191/71‑1 (MQ=255)
                                                                                                           tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGa                                       >  3:317509/1‑149 (MQ=255)
                                                                                                           tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGa                                       >  1:644432/1‑149 (MQ=255)
                                                                                                            cTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                      >  2:796530/1‑149 (MQ=255)
                                                                                                            cTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                      >  3:556672/1‑149 (MQ=255)
                                                                                                             tAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAAt                                     >  2:486320/1‑149 (MQ=255)
                                                                                                                  ccGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGt                                <  2:623858/149‑1 (MQ=255)
                                                                                                                  ccGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGt                                <  1:486320/149‑1 (MQ=255)
                                                                                                                   cGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTg                               <  3:83211/149‑1 (MQ=255)
                                                                                                                   cGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTg                               <  3:361201/149‑1 (MQ=255)
                                                                                                                          gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTAc                        >  3:391582/1‑149 (MQ=255)
                                                                                                                           tttttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACg                       >  3:47343/1‑149 (MQ=255)
                                                                                                                           tttttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACg                       >  3:246554/1‑149 (MQ=255)
                                                                                                                             tttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGtt                     <  4:900567/149‑1 (MQ=255)
                                                                                                                              ttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGTGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTAc                                  <  3:544868/135‑1 (MQ=255)
                                                                                                                              ttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTAc                                  >  4:544868/1‑135 (MQ=255)
                                                                                                                                  aaaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTgcgc                 >  4:699886/1‑149 (MQ=255)
                                                                                                                                    aaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCt               >  3:453053/1‑149 (MQ=255)
                                                                                                                                      aaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTg             <  3:292981/149‑1 (MQ=255)
                                                                                                                                             cccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCt      <  1:172955/149‑1 (MQ=255)
                                                                                                                                              ccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTg     >  3:456392/1‑149 (MQ=255)
                                                                                                                                                tGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGtt   <  4:391582/149‑1 (MQ=255)
                                                                                                                                                 gCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCATTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGttt  <  4:249743/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTT  >  NC_000913/4424321‑4424613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: