Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,439,845 T→A 7.9% intergenic (+52/+27) ytfK → / ← ytfL DUF1107 family protein/UPF0053 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,439,8450TA 7.9% 303.7 / 13.6 89intergenic (+52/+27)ytfK/ytfLDUF1107 family protein/UPF0053 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (38/44);  new base A (3/4);  total (41/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

TAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATCCAC  >  NC_000913/4439697‑4439988
                                                                                                                                                    |                                                                                                                                               
tAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAt                                                                                                                                                 >  3:842312/1‑149 (MQ=255)
 aaGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAtt                                                                                                                                                <  3:321727/149‑1 (MQ=255)
  aGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTGAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAGAGTGCTATGCAGTAATAAGACGGCTCCTGATTc                                                                                                                                               <  1:268447/149‑1 (MQ=255)
    aTTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCCATGCAGTAATAAGACGGCTCCTGATTCAg                                                                                                                                             >  3:378937/1‑149 (MQ=255)
      tCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGa                                                                                                                                           >  2:429406/1‑149 (MQ=255)
      tCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGa                                                                                                                                           <  1:271659/149‑1 (MQ=255)
        ttATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGc                                                                                                                                         <  4:383989/149‑1 (MQ=255)
         tATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGcc                                                                                                                                        <  1:125866/149‑1 (MQ=255)
         tATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGcc                                                                                                                                        <  3:222915/149‑1 (MQ=255)
         tATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGAATCAGGAGcc                                                                                                                                        <  4:316637/149‑1 (MQ=255)
           tCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGt                                                                                                                                      >  3:142938/1‑149 (MQ=255)
              cAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGa                                                                                                                                   >  3:150362/1‑149 (MQ=255)
              cAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGa                                                                                                                                   >  1:594283/1‑149 (MQ=255)
              cAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGa                                                                                                                                   >  4:892714/1‑149 (MQ=255)
              cAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGa                                                                                                                                   >  3:842702/1‑149 (MQ=255)
                 aaGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGt                                                                                                                                <  3:679412/149‑1 (MQ=255)
                 aaGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGt                                                                                                                                <  2:327460/149‑1 (MQ=255)
                     gAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCTTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCt                                                                                                                            <  4:565692/149‑1 (MQ=255)
                     gAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCt                                                                                                                            <  4:842312/149‑1 (MQ=255)
                     gAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCt                                                                                                                            <  1:637732/149‑1 (MQ=255)
                     gAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCt                                                                                                                            <  1:23180/149‑1 (MQ=255)
                      aaGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTg                                                                                                                           <  3:820639/149‑1 (MQ=255)
                        ggATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTggg                                                                                                                         <  1:163986/149‑1 (MQ=255)
                         gATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTgggg                                                                                                                        >  4:739895/1‑149 (MQ=255)
                         gATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTgggg                                                                                                                        <  3:642221/149‑1 (MQ=255)
                           tAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGt                                                                                                                      >  3:787107/1‑149 (MQ=255)
                               cTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTAcg                                                                                                                  >  4:162744/1‑149 (MQ=255)
                                       gTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCtt                                                                                                          >  2:630677/1‑149 (MQ=255)
                                        tCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCttt                                                                                                         <  2:332934/149‑1 (MQ=255)
                                          aGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTttct                                                                                                       >  4:848625/1‑149 (MQ=255)
                                           gTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTttctt                                                                                                      >  2:834437/1‑149 (MQ=255)
                                            tGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTttcttc                                                                                                     <  2:145084/149‑1 (MQ=255)
                                             gATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTtcttct                                                                                                    <  4:15410/149‑1 (MQ=255)
                                                 tCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtctttat                                                                                                <  1:437816/149‑1 (MQ=255)
                                                  ccGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTtctttatc                                                                                               <  4:142938/149‑1 (MQ=255)
                                                        ttAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGc                                                                                         >  1:361825/1‑149 (MQ=255)
                                                            ccGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCa                                                                                     <  3:848625/149‑1 (MQ=255)
                                                             cGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAg                                                                                    <  1:730037/149‑1 (MQ=255)
                                                                cAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCa                                                                                 <  1:151971/149‑1 (MQ=255)
                                                                        gCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGc                                                                         >  4:561604/1‑149 (MQ=255)
                                                                           tCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGaa                                                                      <  1:630677/149‑1 (MQ=255)
                                                                             tGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAg                                                                    <  3:561604/149‑1 (MQ=255)
                                                                                aaaCAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGc                                                                 >  1:719370/1‑149 (MQ=255)
                                                                                aaaCAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGc                                                                 >  1:365426/1‑149 (MQ=255)
                                                                                  aCAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCgg                                                               >  3:157701/1‑149 (MQ=255)
                                                                                   cAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGt                                                              <  3:739895/149‑1 (MQ=255)
                                                                                    agagATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGa                                                                                                               >  1:458953/1‑99 (MQ=255)
                                                                                    agagATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGa                                                                                                               <  2:458953/99‑1 (MQ=255)
                                                                                    agagATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTg                                                             <  3:162744/149‑1 (MQ=255)
                                                                                       gaTGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGcc                                                          >  4:763869/1‑149 (MQ=255)
                                                                                           gCTTAACCAAAGTGCTAGGCAGTAAAAAGTGCTATGCAGTAATAAAACGGCTCGTGATTCAGGAGCCGGTGATGTTTCTGGAGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGc                                                      <  2:901061/149‑1 (MQ=255)
                                                                                            cTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCt                                                     >  4:771746/1‑149 (MQ=255)
                                                                                             ttAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTgggg                                                                                                                        <  4:399577/81‑1 (MQ=255)
                                                                                             ttAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTgggg                                                                                                                        >  3:399577/1‑81 (MQ=255)
                                                                                             ttAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGa                                                                                                               <  2:621679/90‑1 (MQ=255)
                                                                                             ttAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGa                                                                                                               >  1:621679/1‑90 (MQ=255)
                                                                                             ttAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTg                                                    >  4:554180/1‑149 (MQ=255)
                                                                                             ttAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTg                                                    >  3:562612/1‑149 (MQ=255)
                                                                                                 ccAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGa                                                >  3:588093/1‑149 (MQ=255)
                                                                                                 ccAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGa                                                >  4:161324/1‑149 (MQ=255)
                                                                                                 ccAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGa                                                >  3:901203/1‑149 (MQ=255)
                                                                                                      gtgctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCTGTGGCCTTGCCGTCTATCCgg                                           <  4:157701/149‑1 (MQ=255)
                                                                                                      gtgctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCgg                                           <  3:771746/149‑1 (MQ=255)
                                                                                                      gtgctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCgg                                           <  2:594283/149‑1 (MQ=255)
                                                                                                      gtgctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCgg                                           <  3:161324/149‑1 (MQ=255)
                                                                                                      gtgctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCgg                                           <  2:929249/149‑1 (MQ=255)
                                                                                                        gctatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGt                                         <  2:719370/149‑1 (MQ=255)
                                                                                                          tatgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCa                                       >  1:733385/1‑149 (MQ=255)
                                                                                                           atgcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCAc                                      <  1:333859/149‑1 (MQ=255)
                                                                                                             gcagtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACca                                    >  1:251057/1‑149 (MQ=255)
                                                                                                               agtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCAcc                                     >  4:483646/1‑146 (MQ=255)
                                                                                                               agtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCAcc                                     <  3:483646/146‑1 (MQ=255)
                                                                                                               agtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACcagc                                  >  2:65137/1‑149 (MQ=255)
                                                                                                                gtaaaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTTTCGATCCGGGTCACcagca                                 >  4:814683/1‑149 (MQ=255)
                                                                                                                  aaaaagtgccatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCagcagc                               <  4:378937/149‑1 (MQ=255)
                                                                                                                   aaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCt                              <  1:65137/149‑1 (MQ=255)
                                                                                                                   aaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCt                              >  2:126036/1‑149 (MQ=255)
                                                                                                                   aaaagtgctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCt                              <  2:731257/149‑1 (MQ=255)
                                                                                                                          ctatgcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGAt                       >  1:86602/1‑149 (MQ=255)
                                                                                                                              gcagtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCgg                   >  2:367888/1‑149 (MQ=255)
                                                                                                                                agtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTa                 <  1:887193/149‑1 (MQ=255)
                                                                                                                                agtaaTAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTa                 <  3:763869/149‑1 (MQ=255)
                                                                                                                                  taataaGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGt               >  1:683368/1‑149 (MQ=255)
                                                                                                                                      aaGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATc           <  1:690941/149‑1 (MQ=255)
                                                                                                                                         aCGGCTCCTGAATCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGat        >  1:745848/1‑149 (MQ=255)
                                                                                                                                           ggCTCCTGAATCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATg                      >  4:752028/1‑133 (MQ=255)
                                                                                                                                           ggCTCCTGAATCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATg                      <  3:752028/133‑1 (MQ=255)
                                                                                                                                           ggCTCCTGAATCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGatat      >  4:874730/1‑149 (MQ=255)
                                                                                                                                             cTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATcc    <  4:150362/149‑1 (MQ=255)
                                                                                                                                              tcttGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATcca   >  3:232229/4‑149 (MQ=255)
                                                                                                                                               ccTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATccac  <  4:847080/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGTGCTATGCAGTAATAAGACGGCTCCTGATTCAGGAGCCGTTGATGTTTCTGGGGGTTACGCGACGCTTTCTTCTTTATCTTTAGCGTCAGGCAGTTTTGGCGAAAGGGCGGTGGCCTTGCTGTCGATCCGGGTCACCAGCAGCTGGTCGATGCGGTAGTTATCGATATCCAC  >  NC_000913/4439697‑4439988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: