Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,479,020 A→T 6.1% intergenic (+131/+14) rraB → / ← yjgM protein inhibitor of RNase E/GNAT family putative N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,479,0200AT 6.1% 325.6 / 8.1 95intergenic (+131/+14)rraB/yjgMprotein inhibitor of RNase E/GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (49/40);  new base T (2/4);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

CGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATA  >  NC_000913/4478874‑4479165
                                                                                                                                                  |                                                                                                                                                 
cGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACAcg                                                                                                                                                 >  4:670214/1‑149 (MQ=255)
 ggAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACAcgc                                                                                                                                                <  2:706482/149‑1 (MQ=255)
 ggAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACAcgc                                                                                                                                                <  3:625958/149‑1 (MQ=255)
  gAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACAcgcg                                                                                                                                               >  3:810693/1‑149 (MQ=255)
   aGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGTGTTCAACTCGCgg                                                                                                                                              <  4:440522/149‑1 (MQ=255)
   aGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCgg                                                                                                                                              <  1:444170/149‑1 (MQ=255)
    gTTCGCCACTAATTAAGCGTAGCGCATCAGGCACAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGTGTTCAACTCGCggg                                                                                                                                             <  1:910192/149‑1 (MQ=255)
          cACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCAtttt                                                                                                                                       >  2:425599/1‑149 (MQ=255)
             tAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGATAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCAttttttt                                                                                                                                    >  3:511979/1‑149 (MQ=255)
               aTTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTag                                                                                                                                  >  4:54614/1‑149 (MQ=255)
                ttAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTaga                                                                                                                                 >  3:481876/1‑149 (MQ=255)
                ttAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTaga                                                                                                                                 <  1:173886/149‑1 (MQ=255)
                  aaGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGt                                                                                                                               <  1:915325/149‑1 (MQ=255)
                   aGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGtt                                                                                                                              <  2:684412/149‑1 (MQ=255)
                         gcgcATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCa                                                                                                                        >  1:889013/1‑149 (MQ=255)
                            cATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACa                                                                                                                     >  4:157266/1‑149 (MQ=255)
                               cAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATcc                                                                                                                  <  1:483986/149‑1 (MQ=255)
                               cAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATcc                                                                                                                  <  3:157266/149‑1 (MQ=255)
                                aGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCg                                                                                                                 <  3:731869/149‑1 (MQ=255)
                                 ggCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGc                                                                                                                <  2:847572/149‑1 (MQ=255)
                                    ccAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACt                                                                                                             >  1:752362/1‑149 (MQ=255)
                                     cAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACtt                                                                                                            >  3:853294/1‑149 (MQ=255)
                                      aGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTc                                                                                                           >  2:903206/1‑149 (MQ=255)
                                       ggTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGAATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTca                                                                                                          <  1:272992/149‑1 (MQ=255)
                                           gTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAAt                                                                                                      >  2:626993/1‑149 (MQ=255)
                                              ccTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGa                                                                                                   <  3:670214/149‑1 (MQ=255)
                                               cTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTc                                                                                                           <  1:131870/140‑1 (MQ=255)
                                               cTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTc                                                                                                           >  2:131870/1‑140 (MQ=255)
                                               cTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAATTCGCGCAACATCCGCACTTCACAATCGAc                                                                                                  >  1:605992/1‑149 (MQ=255)
                                                 gCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACAt                                                                                                <  1:444839/149‑1 (MQ=255)
                                                    aCCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGc                                                                                             <  3:54614/149‑1 (MQ=255)
                                                    aCCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGc                                                                                             >  2:241289/1‑149 (MQ=255)
                                                     ccTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGca                                                                                            <  2:918749/149‑2 (MQ=255)
                                                      cTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGccc                                                                                           >  4:674277/1‑149 (MQ=255)
                                                          tGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGc                                                                                       >  3:151352/1‑149 (MQ=255)
                                                           gAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCa                                                                                      <  1:26315/149‑1 (MQ=255)
                                                              tttGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGcc                                                                                   >  2:636271/1‑149 (MQ=255)
                                                                  tAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAgc                                                                               <  4:470438/149‑1 (MQ=255)
                                                                  tAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAgc                                                                               <  3:674277/149‑1 (MQ=255)
                                                                    gCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAgcgc                                                                             >  2:654232/1‑149 (MQ=255)
                                                                     cccGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCa                                                                            <  2:605992/149‑1 (MQ=255)
                                                                       cGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATa                                                                          <  1:654232/149‑1 (MQ=255)
                                                                       cGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATa                                                                          <  1:148888/149‑1 (MQ=255)
                                                                         gATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGCTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGt                                                                        >  4:606/1‑149 (MQ=255)
                                                                           tAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCg                                                                      <  1:636271/149‑1 (MQ=255)
                                                                            aaGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGAGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGa                                                                     >  2:674110/1‑149 (MQ=255)
                                                                              ggCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGata                                                                   >  2:816036/1‑149 (MQ=255)
                                                                              ggCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGata                                                                   >  4:62256/1‑149 (MQ=255)
                                                                              ggCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGCTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGata                                                                   <  3:606/149‑1 (MQ=255)
                                                                                 aTTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACCTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGc                                                                >  1:887213/1‑149 (MQ=255)
                                                                                 aTTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGCCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGAGATGc                                                                >  4:7353/1‑149 (MQ=255)
                                                                                  ttCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCt                                                               >  1:687880/1‑149 (MQ=255)
                                                                                     aTGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCaa                                                                                 >  1:696443/1‑128 (MQ=255)
                                                                                     aTGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCaa                                                                                 <  2:696443/128‑1 (MQ=255)
                                                                                     aTGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCaa                                                            >  1:781762/1‑149 (MQ=255)
                                                                                      tGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCaaa                                                           >  4:436657/1‑149 (MQ=255)
                                                                                          acaTCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGccc                                                       >  3:357555/1‑149 (MQ=255)
                                                                                            aTCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCaa                                                     >  3:129670/1‑149 (MQ=255)
                                                                                            aTCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCaa                                                     >  1:423438/1‑149 (MQ=255)
                                                                                            aTCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCACATAGTCGATATGCTCAAAGCCCaa                                                     >  1:283733/1‑149 (MQ=255)
                                                                                             tCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCaaa                                                    <  1:241289/149‑1 (MQ=255)
                                                                                               cGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATg                                                  >  1:748776/1‑149 (MQ=255)
                                                                                                 gCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGCTATGCTCAAAGCCCAAATGCt                                                >  3:907265/1‑149 (MQ=255)
                                                                                                  cATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTc                                               >  1:98975/1‑149 (MQ=255)
                                                                                                  cATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTc                                               <  3:659811/149‑1 (MQ=255)
                                                                                                   aTAAACAGAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCa                                              >  2:507289/1‑149 (MQ=255)
                                                                                                   aTAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCa                                              <  4:151352/149‑1 (MQ=255)
                                                                                                   aTAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCa                                              >  4:530350/1‑149 (MQ=255)
                                                                                                    tAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCAt                                             >  1:47808/1‑149 (MQ=255)
                                                                                                    tAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCAt                                             <  2:889013/149‑1 (MQ=255)
                                                                                                     aaaCAAAAGGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATa                                            >  3:853535/1‑149 (MQ=255)
                                                                                                     aaaCAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATa                                            >  4:163305/1‑149 (MQ=255)
                                                                                                       aCAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCATATGCTCATaaa                                          <  3:800657/149‑1 (MQ=255)
                                                                                                       aCAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATaaa                                          >  2:860655/1‑149 (MQ=255)
                                                                                                       aCAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATaaa                                          <  4:554602/149‑1 (MQ=255)
                                                                                                       aCAAAACGTACATTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATaaa                                          >  1:329618/1‑149 (MQ=255)
                                                                                                               tacgtTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATg                                  >  3:535456/1‑149 (MQ=255)
                                                                                                                  gttgttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCt                               >  2:644816/1‑149 (MQ=255)
                                                                                                                      ttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCt                           >  3:625655/1‑149 (MQ=255)
                                                                                                                      ttAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCt                           <  2:423438/149‑1 (MQ=255)
                                                                                                                       tAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCtt                          <  4:842800/149‑1 (MQ=255)
                                                                                                                        aaTGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCttt                         >  1:848993/1‑149 (MQ=255)
                                                                                                                            ttCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTaaaa                     >  3:128959/1‑149 (MQ=255)
                                                                                                                                aaTGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGc                 <  2:848993/149‑1 (MQ=255)
                                                                                                                                  tGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCgg               <  4:853294/149‑1 (MQ=255)
                                                                                                                                    cccGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTc             <  3:736733/149‑1 (MQ=255)
                                                                                                                                    cccGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTc             <  2:47808/149‑1 (MQ=255)
                                                                                                                                     ccGCGTGTTCAACTCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCaaa                                                    <  4:364333/109‑1 (MQ=255)
                                                                                                                                     ccGCGTGTTCAACTCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCaaa                                                    >  3:364333/1‑109 (MQ=255)
                                                                                                                                     ccGCGTGTTCAACTCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGc                                                 >  4:41309/1‑112 (MQ=255)
                                                                                                                                     ccGCGTGTTCAACTCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGc                                                 <  3:41309/112‑1 (MQ=255)
                                                                                                                                         gAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTc        <  1:860655/149‑1 (MQ=255)
                                                                                                                                         gAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTc        <  1:288498/149‑1 (MQ=255)
                                                                                                                                               aaCACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATa  >  2:557150/1‑149 (MQ=255)
                                                                                                                                               aaCACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATa  <  4:853535/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAGATA  >  NC_000913/4478874‑4479165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: