Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,513,106 C→G 6.1% A102P (GCG→CCG)  fecC ← ferric citrate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,513,1060CG 6.1% 350.8 / 11.5 99A102P (GCG→CCG) fecCferric citrate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (40/53);  new base G (3/3);  total (43/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 101 total aligned reads displayed.

TTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTC  >  NC_000913/4512961‑4513253
                                                                                                                                                 |                                                                                                                                                   
tttCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAgcgccgc                                                                                                                                                  >  4:741219/1‑149 (MQ=255)
      tCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGGCGCGGCGCt                                                                                                                                            <  2:429704/149‑1 (MQ=255)
        aTGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGt                                                                                                                                          <  2:788820/149‑1 (MQ=255)
        aTGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGt                                                                                                                                          <  3:747150/149‑1 (MQ=255)
        aTGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGt                                                                                                                                          <  1:444361/149‑1 (MQ=255)
         tGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGtt                                                                                                                                         >  2:78521/1‑149 (MQ=255)
            gTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAACTCACGCCGCCCCCGCAGGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTAAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGCTAAt                                                                                                                                      <  2:866554/149‑1 (MQ=255)
              aTGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGGCGCGGCGCTggctatgg                                                                                                                                    <  2:914812/149‑8 (MQ=255)
               tGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGcc                                                                                                                                   <  4:203000/149‑1 (MQ=255)
               tGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGcc                                                                                                                                   <  1:410817/149‑1 (MQ=255)
                gACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCg                                                                                                                                  >  1:797454/1‑149 (MQ=255)
                gACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCg                                                                                                                                  >  1:170321/1‑149 (MQ=255)
                gACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCg                                                                                                                                  >  1:815024/1‑149 (MQ=255)
                gACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCg                                                                                                                                  <  4:330441/149‑1 (MQ=255)
                gACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCg                                                                                                                                  >  4:445798/1‑149 (MQ=255)
                 aCGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGa                                                                                                                                 <  3:788272/149‑1 (MQ=255)
                 aCGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGa                                                                                                                                 <  1:749601/149‑1 (MQ=255)
                  cGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAg                                                                                                                                >  1:19307/1‑149 (MQ=255)
                   gAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGc                                                                                                                               >  3:725573/1‑149 (MQ=255)
                   gAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGc                                                                                                                               >  2:897059/1‑149 (MQ=255)
                    aaaTCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCa                                                                                                                              <  1:446347/149‑1 (MQ=255)
                      aTCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGt                                                                                                                            <  3:590133/149‑1 (MQ=255)
                      aTCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGt                                                                                                                            <  1:357621/149‑1 (MQ=255)
                        ccGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGa                                                                                                                          >  3:519930/1‑149 (MQ=255)
                          gCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAg                                                                                                                        <  2:418235/149‑1 (MQ=255)
                          gCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAg                                                                                                                        >  4:923920/1‑149 (MQ=255)
                              cctGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGgaga                                                                                                                    <  2:815024/149‑1 (MQ=255)
                              cctGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGgaga                                                                                                                    <  3:834025/149‑1 (MQ=255)
                               ctGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCTAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGagag                                                                                                                   <  2:501464/149‑1 (MQ=255)
                                tGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAgg                                                                                                                  >  2:860813/1‑149 (MQ=255)
                                 gCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGc                                                                                                                 <  2:298519/149‑1 (MQ=255)
                                     tCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTGAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCAtt                                                                                                             >  4:46637/1‑149 (MQ=255)
                                     tCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCAtt                                                                                                             >  1:316721/1‑149 (MQ=255)
                                     tCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCAtt                                                                                                             <  3:212454/149‑1 (MQ=255)
                                     tCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCAtt                                                                                                             >  1:68826/1‑149 (MQ=255)
                                     tCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCAtt                                                                                                             >  2:559488/1‑149 (MQ=255)
                                      cATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTg                                                                                                            >  1:189909/1‑149 (MQ=255)
                                          aCCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGtt                                                                                                        <  4:546277/149‑1 (MQ=255)
                                             agcagcCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTtgtg                                                                                                     <  3:765899/149‑1 (MQ=255)
                                              gcagcCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTgg                                                                                                    <  1:687303/149‑1 (MQ=255)
                                                 gccagcTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTc                                                                                                 >  3:263236/1‑149 (MQ=255)
                                                 gccagcTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTc                                                                                                 >  3:235461/1‑149 (MQ=255)
                                                  ccagcTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTtgt                                                                                                      <  1:585611/143‑1 (MQ=255)
                                                  ccagcTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTtgt                                                                                                      >  2:585611/1‑143 (MQ=255)
                                                    agcTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAgg                                                                                              <  1:454768/149‑1 (MQ=255)
                                                    agcTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAgg                                                                                              >  4:728664/1‑149 (MQ=255)
                                                       tCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAAt                                                                                                                                      <  4:490024/106‑1 (MQ=255)
                                                       tCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAAt                                                                                                                                      >  3:490024/1‑106 (MQ=255)
                                                        cACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGttt                                                                                          >  1:542129/1‑149 (MQ=255)
                                                         aCGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCa                                                                                                                              <  4:884915/112‑1 (MQ=255)
                                                         aCGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCa                                                                                                                              >  3:884915/1‑112 (MQ=255)
                                                         aCGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTg                                                                                         <  2:96182/149‑1 (MQ=255)
                                                              gccCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTgcagca                                                                                    <  1:643465/149‑1 (MQ=255)
                                                                  ccGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGt                                                                                <  1:860813/149‑1 (MQ=255)
                                                                            cgATGAATGACAGAGAATAGCCTGCAATCGGCGTGGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCCGCGTGCCCGCGAgc                                                                      <  2:846617/149‑1 (MQ=255)
                                                                              atgaatgaCAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGtt                                                                                           >  1:873163/1‑126 (MQ=255)
                                                                              atgaatgaCAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGtt                                                                                           <  2:873163/126‑1 (MQ=255)
                                                                                gaatgaCAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGTTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCa                                                                  <  4:468820/149‑1 (MQ=255)
                                                                                gaatgaCAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCa                                                                  <  2:575740/149‑1 (MQ=255)
                                                                                 aatgaCAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAg                                                                 <  1:559488/149‑1 (MQ=255)
                                                                                      cAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTg                                                            <  2:189909/149‑1 (MQ=255)
                                                                                      cAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTg                                                            >  2:72092/1‑149 (MQ=255)
                                                                                       agagAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTgc                                                           >  1:757815/1‑149 (MQ=255)
                                                                                        gagaATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTgcg                                                          >  3:527088/1‑149 (MQ=255)
                                                                                         agaATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTgcgc                                                         <  1:810428/149‑1 (MQ=255)
                                                                                          gaATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGcc                                                        <  3:428196/149‑1 (MQ=255)
                                                                                              aGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATc                                                    <  4:843035/149‑1 (MQ=255)
                                                                                                   gCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAAc                                               <  1:633118/149‑1 (MQ=255)
                                                                                                    cAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACg                                              <  1:78521/149‑1 (MQ=255)
                                                                                                     aaTCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGt                                                                                >  2:70144/1‑114 (MQ=255)
                                                                                                     aaTCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGt                                                                                <  1:70144/114‑1 (MQ=255)
                                                                                                      aTCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGc                                            <  1:600401/149‑1 (MQ=255)
                                                                                                       tCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCg                                           >  4:250720/1‑149 (MQ=255)
                                                                                                                gACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCtt                                  >  1:350560/1‑149 (MQ=255)
                                                                                                                gACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCtt                                  >  4:727067/1‑149 (MQ=255)
                                                                                                                   tCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGt                               >  2:923405/1‑149 (MQ=255)
                                                                                                                   tCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGt                               >  4:456208/1‑149 (MQ=255)
                                                                                                                   tCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGt                               <  1:72092/149‑1 (MQ=255)
                                                                                                                    cAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTg                              <  3:741219/149‑1 (MQ=255)
                                                                                                                             ggTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGaa                     <  2:858413/149‑1 (MQ=255)
                                                                                                                             ggTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGaa                     <  1:28400/149‑1 (MQ=255)
                                                                                                                              gTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAg                    <  2:19307/149‑1 (MQ=255)
                                                                                                                              gTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAg                    <  1:897059/149‑1 (MQ=255)
                                                                                                                                 aGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGaa                     >  1:692158/1‑145 (MQ=255)
                                                                                                                                 aGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGaa                     <  2:692158/145‑1 (MQ=255)
                                                                                                                                 aGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGtt                 >  4:355238/1‑149 (MQ=255)
                                                                                                                                  gcgcCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGttt                >  3:681946/1‑149 (MQ=255)
                                                                                                                                   cgcCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGtttt               <  4:203430/149‑1 (MQ=255)
                                                                                                                                    gcCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTg              <  2:316721/149‑1 (MQ=255)
                                                                                                                                     ccATAGCCAGCGCCGCGCTGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGc             >  2:269875/1‑149 (MQ=255)
                                                                                                                                      cATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCa            >  3:508483/1‑149 (MQ=255)
                                                                                                                                       aTAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCAc           <  1:233778/149‑1 (MQ=255)
                                                                                                                                        ttaacAGCGGCGCGGCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCAcc          >  3:671816/5‑149 (MQ=255)
                                                                                                                                        tAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGtttt               >  1:441894/1‑144 (MQ=255)
                                                                                                                                        tAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGtttt               <  2:441894/144‑1 (MQ=255)
                                                                                                                                          gCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCACGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAg        >  3:815882/1‑149 (MQ=255)
                                                                                                                                           acAGCGGCGCGGCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACgg                                             <  4:259599/110‑1 (MQ=255)
                                                                                                                                           acAGCGGCGCGGCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACgg                                             >  3:259599/2‑111 (MQ=255)
                                                                                                                                           acAGCGGCGCGGCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAgc       >  3:116764/2‑149 (MQ=255)
                                                                                                                                                gccgcgccgcTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTc  >  3:907273/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGTTGTGGGTCAGGGTTTGCAGCAGCGTGCCCGCGAGCGCCAGGCTTGCGCCGATCAGAACGGCGACCAGGCTTCGTGGCAAACGAAGGTTTTGCACCAGCGCTTC  >  NC_000913/4512961‑4513253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: