Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,725,630 Δ1 bp 88.3% coding (117/1299 nt) kgtP ← alpha‑ketoglutarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,725,6300A.88.3% 529.3 / 76.9 154coding (117/1299 nt)kgtPalpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base A (7/9);  new base . (66/70);  total (74/80)
Fisher's exact test for biased strand distribution p-value = 7.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.67e-01

Only 100 of 154 total aligned reads displayed.

TAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCA  >  NC_000913/2725369‑2725856
                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                  
                            tCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGTTTACCTGAAGAGGccccc                                                                                                                                                                                          >  2:608305/1‑275 (MQ=255)
                                                                               acCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGAAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCt                                                                                                                                       >  1:81296/1‑275 (MQ=255)
                                                                                  gATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGa                                                                                                                                                                                                    >  2:157664/1‑211 (MQ=255)
                                                                                  gATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGc                                                                                                                                     >  4:39174/1‑274 (MQ=255)
                                                                                   aTAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGa                                                                                                                                                                                                                 <  4:354940/198‑1 (MQ=255)
                                                                                      aCAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAagag                                                                                                                                                                                                <  4:157664/211‑1 (MQ=255)
                                                                                        aGCATCGAGTTTTTGCGACCATGTTTTTCGGCTATGCGGCCAAATAGCCAACCGCCGAGTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCAGTTTGAAGGAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAAACGAACCACTCGACCAg                                                                                                                                                                                                             <  2:650537/196‑1 (MQ=255)
                                                                                         cgattggttgtttGGGACCATGTTTAGCGNNTATGCGGACAAATCGCCAACCGCCTATTGGNGGCAACAGGAATCCCGCAGCAAAAACACCGGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCAGGAAGGGAAGAANATGTGGGCAAAGTAGAGTGAACAGAACGAGAAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAg                                                                                                                             <  3:81296/265‑1 (MQ=255)
                                                                                                                   tCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGtctc                                                                                                   <  2:39174/275‑1 (MQ=255)
                                                                                                                       cTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGaaa                                                                                                                                                                                                                              >  7:631377/1‑149 (MQ=255)
                                                                                                                       cTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAAt                                                                                                                                                                                                                             <  8:814349/149‑1 (MQ=255)
                                                                                                                          tGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAACCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGa                                                                                                                                                                                                    >  2:731265/1‑171 (MQ=255)
                                                                                                                           gCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGaa                                                                                                                                                                                                                         <  7:478942/149‑1 (MQ=255)
                                                                                                                              gCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAACCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAagag                                                                                                                                                                                                <  4:731265/171‑1 (MQ=255)
                                                                                                                                cAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACt                                                                                                                                                                                                                    >  8:110159/1‑149 (MQ=255)
                                                                                                                                    tAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTCCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGAc                                                                                                                                                                                                                <  8:911032/149‑1 (MQ=255)
                                                                                                                                      gCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCa                                                                                                                                                                                                              <  7:27381/149‑1 (MQ=255)
                                                                                                                                        cAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGa                                                                                                                                                                                                            <  5:355174/149‑1 (MQ=255)
                                                                                                                                        cAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGa                                                                                                                                                                                                            <  8:781285/149‑1 (MQ=255)
                                                                                                                                         aaCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGa                                                                                                                                                                                                            <  8:631377/149‑1 (MQ=255)
                                                                                                                                         aaCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTc                                                                                                                                                                                                                   <  6:1085379/141‑1 (MQ=255)
                                                                                                                                         aaCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTc                                                                                                                                                                                                                   >  5:1085379/1‑141 (MQ=255)
                                                                                                                                            cGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTAc                                                                                                                                                                                                        >  5:14602/1‑149 (MQ=255)
                                                                                                                                                    gggCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAagag                                                                                                                                                                                                >  5:935988/1‑149 (MQ=255)
                                                                                                                                                     ggCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAaga                                                                                                                                                                                                 <  5:1039926/147‑1 (MQ=255)
                                                                                                                                                      gcgcATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGc                                                                                                                                                                                              <  6:364844/149‑1 (MQ=255)
                                                                                                                                                       cgcATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGa                                                                                                                                                                                                            >  4:526701/1‑134 (MQ=255)
                                                                                                                                                        gcATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGccc                                                                                                                                                                                            >  7:1012950/1‑149 (MQ=255)
                                                                                                                                                         cATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGccc                                                                                                                                                                                            <  6:98493/149‑1 (MQ=255)
                                                                                                                                                         cATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGAAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGttt                                                             <  2:528152/275‑1 (MQ=255)
                                                                                                                                                          aTCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTAt                                                                                                                                                              <  2:378453/177‑1 (MQ=255)
                                                                                                                                                           tCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAg                                                                                                                                                                                                             <  4:674054/129‑1 (MQ=255)
                                                                                                                                                               gAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAAt                                                                                                                                                                                     >  5:11181/1‑149 (MQ=255)
                                                                                                                                                               gAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGAGGGGGGCAAAGTAGAGGGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAAt                                                                                                                                                                                     >  8:347514/1‑149 (MQ=255)
                                                                                                                                                                  tCCCGCAGCAAAAACACCTGCTGTTTGGAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAGGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGc                                                                                                                                                                                  <  6:721763/149‑1 (MQ=255)
                                                                                                                                                                   cccGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGcc                                                                                                                                                                                 <  8:31613/149‑1 (MQ=255)
                                                                                                                                                                    ccGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGccc                                                                                                                                                                                <  7:65008/149‑1 (MQ=255)
                                                                                                                                                                    ccGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGccc                                                                                                                                                                                >  8:132364/1‑149 (MQ=255)
                                                                                                                                                                     cGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGt                                                  <  4:239438/274‑1 (MQ=255)
                                                                                                                                                                      gcagcaAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCaa                                                                                                                                                                              >  7:806032/1‑149 (MQ=255)
                                                                                                                                                                       cagcaAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCaaa                                                                                                                                                                             <  7:99827/149‑1 (MQ=255)
                                                                                                                                                                        agcaAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAg                                                                                                                                                                                                             <  2:268311/116‑1 (MQ=255)
                                                                                                                                                                         gcaAAAACACCTGCTGTTTGTATTAGTTGTGTCGTCGGGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAgg                                             <  4:427606/275‑1 (MQ=255)
                                                                                                                                                                         gcaAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAgg                                             >  2:513154/1‑275 (MQ=255)
                                                                                                                                                                         gcaAAAACACCTGCTGTTTGTAGTAGTTGAGGCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAgg                                             <  1:146903/275‑1 (MQ=255)
                                                                                                                                                                          caAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgc                                                                                                                                                                          >  8:139481/1‑149 (MQ=255)
                                                                                                                                                                           aaaaaCACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgcg                                                                                                                                                                         >  7:240702/1‑149 (MQ=255)
                                                                                                                                                                            aaaaCACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgcg                                                                                                                                                                         >  7:941891/1‑149 (MQ=255)
                                                                                                                                                                             aaaCACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCACATgcggc                                                                                                                                                                       >  8:532926/1‑149 (MQ=255)
                                                                                                                                                                               acacCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcga                                                                                                                                                                     >  5:826369/1‑149 (MQ=255)
                                                                                                                                                                                  ccTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGaa                                                                                                                                                                                                                               <  7:140863/88‑1 (MQ=255)
                                                                                                                                                                                  ccTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcgacga                                                                                                                                                                  <  6:600026/149‑1 (MQ=255)
                                                                                                                                                                                  ccTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcgacga                                                                                                                                                                  >  6:247725/1‑149 (MQ=255)
                                                                                                                                                                                  ccTGCTGTACGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGaa                                                                                                                                                                                                                               >  8:140863/1‑88 (MQ=255)
                                                                                                                                                                                     gctgTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGt                                                                                                                                                                <  5:659021/149‑1 (MQ=255)
                                                                                                                                                                                     gctgTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTa                                                                                                                                                               >  6:811951/1‑149 (MQ=255)
                                                                                                                                                                                        gTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAATAAGATGTGGGGAAAGTAGACTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTAACa                                                                                                                                                            <  6:1088091/149‑1 (MQ=255)
                                                                                                                                                                                            gtagtaGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCactac                                                                                                                                                        <  7:110159/149‑1 (MQ=255)
                                                                                                                                                                                              agtagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACtt                                                                                                                                                      <  7:142496/149‑1 (MQ=255)
                                                                                                                                                                                               gtagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTAGtt                                                                                                                                                      >  7:718336/1‑149 (MQ=255)
                                                                                                                                                                                                tagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGt                                                                                                                                                    >  6:251093/1‑149 (MQ=255)
                                                                                                                                                                                                  gtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCa                                                                                                                                                  <  6:14602/149‑1 (MQ=255)
                                                                                                                                                                                                   ttGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGttt                                                             >  2:45598/1‑233 (MQ=255)
                                                                                                                                                                                                   ttGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCACCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCCTTCCATTATGTCTCCTGCCGTAATcc                                                                                       >  1:93479/1‑207 (MQ=255)
                                                                                                                                                                                                        tcgtggGGTTCCCGGAAGGGAAGAAAATTTGGGAAAAGTAGAGTGAACAGACCGAGT‑GAGCTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATg              <  4:608305/275‑1 (MQ=255)
                                                                                                                                                                                                        tcgtcgTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGccccc                                                                                                                                                                                          >  5:246427/1‑104 (MQ=255)
                                                                                                                                                                                                            cgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTc                                                                                                                                        <  8:263430/149‑1 (MQ=255)
                                                                                                                                                                                                             gtgtTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCt                                                                                                                                       >  6:1002832/1‑149 (MQ=255)
                                                                                                                                                                                                                  cccGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGt                                                                                                                                  <  8:1012950/149‑1 (MQ=255)
                                                                                                                                                                                                                    cGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGtt                                                                                                                                 <  8:718336/149‑1 (MQ=255)
                                                                                                                                                                                                                    cGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTa                                                                                                                                >  8:44084/1‑149 (MQ=255)
                                                                                                                                                                                                                     ggaagggaagAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCa  <  4:513154/274‑1 (MQ=255)
                                                                                                                                                                                                                      gaagggaagAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACa                                                                                                                              <  8:541967/149‑1 (MQ=255)
                                                                                                                                                                                                                       aagggaagAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAg                                                                                                                             <  5:811951/149‑1 (MQ=255)
                                                                                                                                                                                                                       aagggaagAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAg                                                                                                                             >  6:12687/1‑149 (MQ=255)
                                                                                                                                                                                                                          ggaagAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGa                                                                                                                                                                                                                 >  2:679101/1‑62 (MQ=255)
                                                                                                                                                                                                                             agaagaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACttt                                                                                                                       <  7:65268/149‑1 (MQ=255)
                                                                                                                                                                                                                              gaagaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAg                                                                                                                                                                                                             <  4:679101/62‑1 (MQ=255)
                                                                                                                                                                                                                              gaagaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTc                                                                                                                      >  5:726270/1‑149 (MQ=255)
                                                                                                                                                                                                                              gaagaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTc                                                                                                                      >  7:636276/1‑149 (MQ=255)
                                                                                                                                                                                                                               aagaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCa                                                                                                                     >  7:715667/1‑149 (MQ=255)
                                                                                                                                                                                                                                     tgGGCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGa                                                                                                                                                                                                    >  2:270157/1‑64 (MQ=255)
                                                                                                                                                                                                                                      gggCAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGt                                                                                                                                         >  7:402806/1‑122 (MQ=255)
                                                                                                                                                                                                                                         cAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAagag                                                                                                                                                                                                <  4:270157/64‑1 (MQ=255)
                                                                                                                                                                                                                                         cAAAGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccatgccat                                                                                                           <  7:940389/149‑1 (MQ=255)
                                                                                                                                                                                                                                           aaGTAGAGTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCAACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTGCAGCCATGCCATTa                                                                                                         <  6:429028/149‑1 (MQ=255)
                                                                                                                                                                                                                                                gagTGAACAGAACGAGTAGACATAGAAATCGAACTACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGtc                                                                                                     >  8:994716/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 agTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTTCCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGtctc                                                                                                   <  5:323664/149‑1 (MQ=255)
                                                                                                                                                                                                                                                 agTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGtctc                                                                                                   >  7:3466/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  gTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTcc                                                                                                  >  7:448969/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  gTGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTcc                                                                                                  >  5:630882/1‑149 (MQ=255)
                                                                                                                                                                                                                                                   tGAACAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCt                                                                                                 <  6:685261/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      aCAGAACGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGcc                                                                                              >  7:1044739/1‑149 (MQ=255)
                                                                                                                                                                                                                                                          aaCGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGt                                                                                                      >  8:275974/1‑137 (MQ=255)
                                                                                                                                                                                                                                                          aaCGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGt                                                                                                      <  7:275974/137‑1 (MQ=255)
                                                                                                                                                                                                                                                          aaCGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTcc                                                                                                  >  6:752892/1‑141 (MQ=255)
                                                                                                                                                                                                                                                          aaCGAGTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTaa                                                                                          >  8:597011/1‑149 (MQ=255)
                                                                                                                                                                                                                                                           aCGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTaa                                                                                          <  8:941891/149‑1 (MQ=255)
                                                                                                                                                                                                                                                               gTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGa                                                                                     <  8:715667/149‑1 (MQ=255)
                                                                                                                                                                                                                                                               gTAGAC‑TAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAat                                         >  2:702833/1‑193 (MQ=255)
                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                  
TAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCA  >  NC_000913/2725369‑2725856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑