Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,799,370 | Δ1 bp | 5.7% | intergenic (‑100/+23) | pheM ← / ← rplT | phenylalanyl‑tRNA synthetase operon leader peptide/50S ribosomal subunit protein L20 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,799,370 | 0 | A | . | 5.7% | 866.4 / 39.2 | 245 | intergenic (‑100/+23) | pheM/rplT | phenylalanyl‑tRNA synthetase operon leader peptide/50S ribosomal subunit protein L20 |
Reads supporting (aligned to +/- strand): ref base A (112/117); new base . (8/6); total (121/124) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.93e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-02 |
Only 100 of 248 total aligned reads displayed.
CGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTAAGAAATACCGTTCTGACGTGCTGCTGCGTTGATACGCGCAATCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATA > NC_000913/1799103‑1799633 | cTGATGAGACAGGCTTTTTATTTTTCTAAACGCTCATATAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCTTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAAc < 2:78548/274‑1 (MQ=255) agaCAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTa > 1:198121/1‑275 (MQ=255) cTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCATTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGAGGGCTTTGAAACGAAAAGAGGGAGCCTCG‑CTCCCTCTGTCAACTGGCTTAtcacaaa > 4:591214/1‑269 (MQ=255) tttCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTACAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTCCCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATACAAGTCAATGTTGTGATGTCGTTGAAACGAAAAGAGGGAGACTAC‑CTCCCTTTTTCAACTCGCTTATGCCATAGCTGCTTTc > 2:638834/1‑275 (MQ=255) tttCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGcc > 2:106231/1‑263 (MQ=255) cgccACTGGGAGGCTTTACGTGGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACg < 4:636543/272‑1 (MQ=255) ctcACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACgg < 1:83675/274‑1 (MQ=255) cgcTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTTNGATACNNGCCAGTACCTTTCGGTT‑GAttt > 3:3725/1‑275 (MQ=255) aGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCa < 1:3725/273‑1 (MQ=255) cctcctGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACag < 2:227140/275‑1 (MQ=255) ttCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTa > 7:247707/1‑149 (MQ=255) ttCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTa < 5:181618/149‑1 (MQ=255) aGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑Ct > 7:729669/1‑149 (MQ=255) gTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTcc < 5:326076/149‑1 (MQ=255) gTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAgctgct > 2:682868/1‑179 (MQ=255) tAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCctct > 6:1050489/1‑149 (MQ=255) aaaGTAAAAAAAGAAGCGGAAAATATCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAG‑CTAGTCTCCCTCtt < 7:644631/149‑1 (MQ=255) aaaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCtt < 8:420493/149‑1 (MQ=255) aaaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCtt < 8:897209/149‑1 (MQ=255) aaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCttt < 8:247707/149‑1 (MQ=255) aaaaaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACt > 8:397384/1‑149 (MQ=255) aagCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCt < 1:125966/274‑1 (MQ=255) agCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCt > 4:243467/1‑273 (MQ=255) gAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGaa < 2:720304/182‑1 (MQ=255) tAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGCTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAgctg > 8:702527/1‑149 (MQ=255) attcattGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGAc < 7:198357/107‑1 (MQ=255) attcattGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGc < 8:1029226/149‑1 (MQ=255) ttcattGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCt > 7:1030326/1‑149 (MQ=255) cattGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCgg > 2:665253/1‑163 (MQ=255) attGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCtttt < 6:678981/149‑1 (MQ=255) attGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCGACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCtttt > 6:944060/1‑149 (MQ=255) cttgtGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCctct < 5:659746/110‑1 (MQ=255) cttgcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCttt > 6:179724/1‑144 (MQ=255) gcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTc < 6:127198/110‑1 (MQ=255) gTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCaga < 6:229270/149‑1 (MQ=255) ttACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTAAGAAATACCGTTCTGACGTGCTGCTTCg > 2:178119/1‑274 (MQ=255) ttttGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTg > 6:635509/1‑149 (MQ=255) ggTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACg > 6:355051/1‑149 (MQ=255) aCTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTa > 7:170164/1‑149 (MQ=255) ctctTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTTAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCATCAGAGGNnttttt > 3:110577/1‑209 (MQ=255) ctctTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGCCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGACCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTAAGAAATACTGTTCTGACGTGCTGCTGCGTTGATACGCGCAAt > 2:388744/1‑275 (MQ=255) ttCAAAAGACCTTTATTGAAAAGGCTACGGTGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACttt > 5:811269/1‑149 (MQ=255) ttCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACttt > 7:690060/1‑149 (MQ=255) ttCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACttt > 8:141976/1‑149 (MQ=255) ttCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACttt < 5:1050489/149‑1 (MQ=255) tCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAgg < 1:110577/209‑1 (MQ=255) cAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGt < 7:235955/149‑1 (MQ=255) cAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAAtt > 1:99992/1‑221 (MQ=255) cAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTAAGAAATACCGTTCTGa > 4:101667/1‑244 (MQ=255) cAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACGGGCTTATGCCAGAGCTGCTTTCTCTTTTTCAACCAGAGCGGTGAACGCTACTTTGt < 6:582407/149‑1 (MQ=255) aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACt < 4:737371/144‑1 (MQ=255) aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc < 7:1053410/149‑1 (MQ=255) aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc < 6:114394/149‑1 (MQ=255) cTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACt > 7:864537/1‑149 (MQ=255) cTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATTAATTTGCTTTAAGAAATACCGTTCTGACTTTCTGCTGCGTTGCTACGCGCAATCCACACTTGACgg > 2:83995/1‑275 (MQ=255) cTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTAAGAAATACCGTTCTGACGTGCTGCTGCGTTGATACGCGCAATCCACAGTTGACgg > 4:508809/1‑275 (MQ=255) cTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAA‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTAAGAAATCCCGTTCTGACGTGCTGCTGCGTTGATACGCGCAATCCACAGTTGACgg > 2:115567/1‑275 (MQ=255) tttATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTg < 5:355051/149‑1 (MQ=255) ttATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAgctgct > 2:554591/1‑102 (MQ=255) ttATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGc < 5:433080/149‑1 (MQ=255) ttATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTAAGAAATACCGTTCTGACGTGCTGCGGCGTTGATACGCCAAATCCAAAGTTTACGGac > 4:490110/1‑274 (MQ=255) tGANAAGGNTACGGCGATANAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCt < 4:704847/212‑1 (MQ=255) tGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTAAGAAATACCGTTCTGACGTGCTGCTGCGTTGATACGCGCAATCca < 2:490110/259‑1 (MQ=255) tGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAG‑CTAGTCTCCctc < 5:942037/71‑1 (MQ=38) aaGGCTACGGCGATAAAAGTGAATGTTTTGATGGCGTGGAAACGAAAAGAGGGAGACTTG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTAAGAAATACCGTTCTGACGTGCTGCTGCGTTGATACGCGCAATCCACAGTTGACGGAACTGACGCt < 4:388744/275‑1 (MQ=255) aaGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACNGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTAATANATACCGTTCTGACGTGCTGCTGCGTTGATANNGGTAATTCAAAGTTGACGGaagcgaacgt > 3:92095/1‑267 (MQ=255) aGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGc < 8:864537/149‑1 (MQ=255) gCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCa > 8:109234/1‑149 (MQ=255) cGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGAt < 6:365343/149‑1 (MQ=255) cGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCtt < 7:994122/149‑1 (MQ=255) aTAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTAc > 8:607874/1‑149 (MQ=255) tAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCa > 2:365414/1‑159 (MQ=255) aGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACag < 4:365414/159‑1 (MQ=255) gTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑Ga < 7:830994/149‑1 (MQ=255) aTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTAAGAAATACCGTTCTGACGTGCTGCTGCGTTGATACGCGCAATCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGt < 2:304951/274‑1 (MQ=255) gTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCg < 3:1293/74‑1 (MQ=255) ttttGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAAc > 7:398435/1‑149 (MQ=255) ttttGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAAc > 7:477896/1‑149 (MQ=255) ggCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGa > 8:49448/1‑111 (MQ=255) gCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACGAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGcc < 6:957482/118‑1 (MQ=255) gCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGcc > 5:957482/1‑118 (MQ=255) gCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCCTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCtt > 5:837831/1‑149 (MQ=255) gTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTAAGAAATACCGTTCTGACGTGCTGCTGCGTTGATACGCGCAATCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGAcc > 2:597085/1‑274 (MQ=255) ttGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCttttt > 5:176893/1‑149 (MQ=255) ttGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCttttt > 8:32791/1‑149 (MQ=255) gAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTc > 8:819231/1‑149 (MQ=255) aaCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAg < 8:539895/149‑1 (MQ=255) aaCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAg < 7:317480/149‑1 (MQ=255) aaCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAg < 6:429943/149‑1 (MQ=255) gAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGcc < 7:573210/149‑1 (MQ=255) taaaGGGGGTGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTAAGAAATACCGTTCTGACGTGCTGCTGCGTTGATACGCGCAATCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATa < 4:178119/274‑1 (MQ=255) gaaaGAGGGAG‑CTAGTCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCa > 7:379534/2‑149 (MQ=255) aaaaGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTaa > 4:666481/1‑167 (MQ=255) aaaGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCAt > 5:465172/1‑149 (MQ=255) agagGGAG‑CTAGTCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATtg > 5:534206/1‑149 (MQ=255) agagGGAG‑CTAGTCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATtg > 8:754246/1‑149 (MQ=255) agGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATtgat < 7:328132/149‑1 (MQ=255) agGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATtgat < 6:811269/149‑1 (MQ=255) gggAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATtgatg > 6:433447/1‑149 (MQ=255) aCTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCa > 1:131846/1‑138 (MQ=255) | CGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCACGCGCTAGCCTCCTGGATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTC‑GATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTTGCTGTAAGAAATACCGTTCTGACGTGCTGCTGCGTTGATACGCGCAATCCACAGTTGACGGAACTGACGCTTACGTTGACGACGGTCACGGTAAGCATACTGACCAGCTTTGATA > NC_000913/1799103‑1799633 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |