Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,330,396:1 | +T | 6.0% | intergenic (+15/+21) | sohB → / ← yciN | inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,330,396 | 1 | . | T | 6.0% | 878.3 / 53.3 | 218 | intergenic (+15/+21) | sohB/yciN | inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN |
Reads supporting (aligned to +/- strand): ref base . (108/97); new base T (3/10); total (111/107) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.68e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01 |
Only 100 of 223 total aligned reads displayed.
CTTCTCTGGATATTGAACAGGTGGCAACGGGTGAACACTGGTACGGACAACAGGCGGTAGAGAAAGGCCTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCGTATCCTCATGCTCACGAATGATTTTGTTGGCCTCTTTCAACAGCGTTTCGCGGTCGATGGGTTGTGTCTCT > NC_000913/1330131‑1330660 | tGAACAGGTGGCAACGGGTGAACACTGGTACGGACAACAGGCGGTAGAGAAAGGCCTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCttt > 2:13903/1‑275 (MQ=255) gTGGCAACGGGTGAACACTGGTACGGACAACAGGCGGTAGAGAAAGGCCTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCATCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAGACGCGAGG‑CTACGACCTCGCGTTTTGCTTTACTCAAc > 2:499478/1‑275 (MQ=255) aCGGGTGAACACTGGTACGGACGACAGGCGGTATAGAAAGGCCTGGTATATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTTATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAGCCAgatg < 4:173053/275‑1 (MQ=255) tGGTACGGACAACAGGCGGTAGAGAAAGGCCTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGa > 2:479683/1‑275 (MQ=255) aaaacaGGCGGTAGAGAAAGGCCTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGcaca < 2:566055/272‑1 (MQ=255) aagagctgtgtgatgaAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTTACCGCTTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACTCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGGGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGa < 4:499478/263‑1 (MQ=255) aaGGCCTGGTTGATGAAATCAACACAAGGTATGAAGTGTTTCTTAGCCTGATGGAATGCCGTGAAGTGGTCAATGTACGCTATTTGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAAc < 4:492135/275‑1 (MQ=255) ggCCTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGca < 2:236642/253‑1 (MQ=255) ccTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACata > 2:332317/1‑274 (MQ=255) ggTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGCGCGCCGATCGATTGTTACTACGCTCGTGGCAGCTGGGTCAACATCCATTAATGTAAAAGACCAACGCAAGG‑CTAAGACCTCGACTATTGCTCTAATCAACCCGAGGATATTTTTCTTAAAGCACATTGTCCAGGTTTTCGCACATATTaa > 4:116087/1‑275 (MQ=255) tgaaaacCAGGTATGAAGTTATGCTTAGCGTGATGGAAGGCCGTTAAGTGGTCAATGTAAGCGATATGCAGCGTAAACGACTCATTGACCGATTCACAGGCAGCGCGGCAGAGAGAGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATGTATCTGAAAGCACATGGGCCa < 1:13507/241‑1 (MQ=255) gTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCt > 1:16647/1‑275 (MQ=255) tgatgaAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTg > 2:468735/1‑275 (MQ=255) aTTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGCGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGATTTTGGCTGTCGGAAAAcc > 4:143765/1‑275 (MQ=255) gtggcTGATGGAAGGGCGGGAAGTGGTCAATGTAAGCGATATGCAGCGTAAACGACTTCTTGGCCGGTTCACCGGCAGAGCGGCAGAGGGCGGCGAGCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAGCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGtt < 4:297570/271‑1 (MQ=255) aaGGCCGTGAAGTGGTCAATGTACGCTATATGCATCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAgg > 6:159524/1‑149 (MQ=255) aaGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAgg > 6:246623/1‑149 (MQ=255) aGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑c > 6:484103/1‑149 (MQ=255) gTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCACTTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTTCTTTTGGCTGTTGGCAATCCTTGTTCATCAAAAAAgtcgtc > 4:487913/1‑274 (MQ=255) tGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGTAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGAc < 6:346609/149‑1 (MQ=255) tGAAGTGGTCAATGTACACTATATACAGCGAAAACTACTCATTGACCGATTCAACGGCAGGGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGc < 1:90089/275‑1 (MQ=255) gAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGAcc < 7:100376/149‑1 (MQ=255) gTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGt > 6:885546/1‑149 (MQ=255) tCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGtt < 5:429315/149‑1 (MQ=255) cAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGttt < 6:488152/149‑1 (MQ=255) cAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGttt < 5:283813/149‑1 (MQ=255) cAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGttt < 8:539496/149‑1 (MQ=255) aaTGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGtttt > 8:619581/1‑149 (MQ=255) aaTGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGtttt < 6:622679/149‑1 (MQ=255) tACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGtttgtctt > 6:297706/1‑144 (MQ=255) aCGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGGGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCa < 2:173862/275‑1 (MQ=255) aCGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGTTTGTCttt < 5:297706/149‑1 (MQ=255) cGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTGAAAGACAAACGCGAGGTCTTAG‑CCTCGCGtttgtctttt < 8:894421/149‑8 (MQ=255) aggtataTGAAGGGTAAACAACTCATTGACCTATTCACCGGAAGGGCGGCAGAGAGCGCCGATTGAGTGTTGCTACGCGGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCa < 4:241571/271‑1 (MQ=255) gCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTaa < 8:878812/149‑1 (MQ=255) tataTGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATc > 5:474370/1‑149 (MQ=255) atGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCCCCGGTAAATCCCAGCACGccct > 4:465443/1‑272 (MQ=255) cAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAg > 5:854991/1‑149 (MQ=255) gCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAgat > 8:365340/1‑149 (MQ=255) gCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTTTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCCCCGGTACATCCCCGCACCCAATTACGc > 4:82847/1‑274 (MQ=255) cGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt > 2:39830/1‑159 (MQ=255) gTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAgatga > 5:784502/1‑149 (MQ=255) gTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTg > 4:42061/1‑274 (MQ=255) aaaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAACAGCCATTGATGTAAAAGACAAACGCGCGG‑CTAAGACCTCGCGTTTTGCTTTAATCCACCAAATAATATTTTTCTGAAAGCACATgg > 2:695016/1‑168 (MQ=255) aaaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGata > 7:787905/1‑149 (MQ=255) aaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAAc < 4:356944/182‑1 (MQ=255) aCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAtt < 5:616696/149‑1 (MQ=255) tCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGATG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt < 6:936919/149‑1 (MQ=255) tCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGTTTGTCttt > 8:294288/1‑126 (MQ=38) tCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt < 8:788628/149‑1 (MQ=255) tCAGTGACCGATTCGCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt > 6:1001802/1‑149 (MQ=255) tGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGaaa > 6:453854/1‑149 (MQ=255) cGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGcac > 5:168446/1‑149 (MQ=255) cGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGcac > 5:87401/1‑149 (MQ=255) gATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGcaca > 5:746716/1‑149 (MQ=255) aTTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACAt > 6:669935/1‑149 (MQ=255) aCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAAc > 5:107245/1‑119 (MQ=255) cAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGgt < 5:157622/149‑1 (MQ=255) cAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGa < 2:528362/275‑1 (MQ=255) cgcgGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGtt < 6:703710/149‑1 (MQ=255) gcgGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGttt < 7:146284/149‑1 (MQ=255) gCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGaa < 5:188348/149‑1 (MQ=255) agCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCACTCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTCCGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCGTATCCTCATg > 2:95376/1‑275 (MQ=255) gcgcCGATCGATTGTTACTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTa > 7:465147/1‑149 (MQ=255) gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTc > 5:485474/1‑113 (MQ=255) cgatcgatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCgg > 2:445701/1‑239 (MQ=255) gatTGTTGCTACGCTGGTGACAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACcgcg > 7:893056/1‑149 (MQ=255) atTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAtttt > 6:575458/1‑103 (MQ=255) ttgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCt > 8:757503/1‑149 (MQ=255) tACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTg > 8:219910/1‑149 (MQ=255) tACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTg < 6:202417/149‑1 (MQ=255) gCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCGTATCCTCATGCTCACGAATGATTTTGTTGGcc < 4:534675/275‑1 (MQ=255) ggtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAATTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGtt < 6:1097182/149‑1 (MQ=255) gtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAacac > 4:218629/1‑129 (MQ=255) gtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCGTATCCTCATGCTCACGAATGATTTTGTTGGCctct > 4:471851/1‑274 (MQ=255) cGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGCGACGCCGGTGGCTTCAATCCCt > 2:607863/1‑225 (MQ=255) gggTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCtt < 6:87401/149‑1 (MQ=255) gTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCa < 4:63565/96‑1 (MQ=255) gTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGt > 7:32498/1‑149 (MQ=255) gTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGa < 4:607863/225‑1 (MQ=255) cAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGATTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTc < 5:325472/149‑1 (MQ=255) aaaGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGAAGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCAt > 7:249445/1‑149 (MQ=255) aGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTTATCAACCCGATGATACTTTTCTGAAAGCACATGGGTCACGTGTTTGGACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATTCTTATTCATCt > 6:799156/1‑149 (MQ=255) aTTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaa > 7:556083/1‑149 (MQ=255) aTTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaa > 7:966174/1‑149 (MQ=255) ttGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa < 5:252785/149‑1 (MQ=255) ttGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa < 7:835492/149‑1 (MQ=255) tGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACGGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCGTATCCTCATGCTCACGAATGATTTTGTTGGCCTCTTTCAACAGCGTTTCGCGGTCGAt < 4:332317/274‑1 (MQ=255) tAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTc > 8:102112/1‑149 (MQ=255) aaaaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg > 7:496637/1‑149 (MQ=255) aagtacacacGCGGGG‑CTATGACCTCGAGTATTGCTTTAATCAACCAGATGATATTTTTCTGAAAGACCATGGGACAGGTGTTTTACCAGATTAAACACCGCGGGGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTCCTCTGCGTGACGCCGGTGGCTTCAAGCCCTGCGAGCGTATCCTCATGCTCACGAATGATTTTGTTGGCCTCTTTCAACAGCGTTTCGCGGTCGATGGGTtgtg < 4:558700/271‑1 (MQ=255) aaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGcc > 5:924350/1‑149 (MQ=255) aaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGcc > 6:176198/1‑149 (MQ=255) aGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATc > 5:583976/1‑133 (MQ=255) cAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATa > 2:298272/1‑153 (MQ=255) aaaCGCGAGGTCTTAG‑CCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTaa > 7:355527/1‑149 (MQ=255) aaaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGttt > 3:109192/1‑78 (MQ=255) cgcgAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCgg < 2:539899/179‑1 (MQ=255) gcgAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAAc < 1:109192/78‑1 (MQ=255) gAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCa < 7:365340/149‑1 (MQ=255) | CTTCTCTGGATATTGAACAGGTGGCAACGGGTGAACACTGGTACGGACAACAGGCGGTAGAGAAAGGCCTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCGTATCCTCATGCTCACGAATGATTTTGTTGGCCTCTTTCAACAGCGTTTCGCGGTCGATGGGTTGTGTCTCT > NC_000913/1330131‑1330660 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |