Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,775,093 G→A 5.7% intergenic (+72/‑452) yfjW → / → ypjI CP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,775,0930GA 5.7% 481.3 / 11.4 151intergenic (+72/‑452)yfjW/ypjICP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (71/71);  new base A (5/4);  total (76/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 151 total aligned reads displayed.

GTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCCAGCAGCTGTTGAATGGAGTACTGTAGGCAAATGGTGCCGTAACCGGCTTTCGTACTGAAAGGTTCTGTCTATCCCATCCCTTCTCTTGTATCTAACATTTAGTTGTGAATAGCTA  >  NC_000913/2774942‑2775353
                                                                                                                                                       |                                                                                                                                                                                                                                                                    
     ggTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCa                                                                                                                                                                                                                                                                    <  8:305064/149‑1 (MQ=255)
     ggTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCa                                                                                                                                                                                                                                                                    <  8:676225/149‑1 (MQ=255)
     ggTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCa                                                                                                                                                                                                                                                                    <  6:540740/149‑1 (MQ=255)
      gTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAg                                                                                                                                                                                                                                                                   <  8:532280/149‑1 (MQ=255)
          gCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCtt                                                                                                                                                                                                                                                               <  8:1009282/149‑1 (MQ=255)
           cTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTg                                                                                                                                                                                                                                                              >  8:828851/1‑149 (MQ=255)
             aGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGCTTTAAAAACAGGCttaac                                                                                                                                                                                                                                                            <  7:687612/149‑4 (MQ=255)
              gCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGc                                                                                                                                                                                                                                                           <  6:627962/149‑1 (MQ=255)
               cAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGTATTTGGCTTCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCGTGTTTTTTAAGCAGGCTTGAGCg                                                                                                                                                                                                                                                          >  6:819851/1‑149 (MQ=255)
                 aTTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTg                                                                                                                                                                                                                                                        >  8:546747/1‑149 (MQ=255)
                      tataAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCg                                                                                                                                                                                                                                                   >  6:362566/1‑149 (MQ=255)
                                 ttcattcTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATAc                                                                                                                                                                                                                                        <  5:355496/149‑1 (MQ=255)
                                   cattcTTAAAGTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTACTTATTAAAATATCCTAACTTAAGCCTGTTATTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCt                                                                                                                                                                                                                                      >  8:345263/1‑149 (MQ=255)
                                       cTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCtt                                                                                                                                                                                                                                  <  6:756222/149‑1 (MQ=255)
                                        ttACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCttt                                                                                                                                                                                                                                 >  6:676049/1‑149 (MQ=255)
                                           cTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTaaa                                                                                                                                                                                                                              <  5:631333/149‑1 (MQ=255)
                                           cTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTaaa                                                                                                                                                                                                                              <  8:604835/149‑1 (MQ=255)
                                              tAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTCTTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGc                                                                                                                                                                                                                         >  2:171990/1‑151 (MQ=255)
                                                 gAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCAt                                                                                                                                                                                                                                          <  6:655148/131‑1 (MQ=255)
                                                 gAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCAt                                                                                                                                                                                                                                          >  5:655148/1‑131 (MQ=255)
                                                 gAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCg                                                                                                                                                                                                                        >  5:452002/1‑149 (MQ=255)
                                                  aaTGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGttt                                                                                                                                                                                                                     <  4:171990/151‑1 (MQ=255)
                                                    tgatgTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCCAGCAGCTGtt                                                                                                           <  4:250733/255‑1 (MQ=255)
                                                      atgTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTTAGCGTGAAGCGGCCACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATTCCAACCCAGCGCCATGGAAGCGCCATCAGCGTTTTAATGGCGtctttcccctata                                                                                      >  4:629547/1‑262 (MQ=255)
                                                      atgTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGa                                                                                                                                                                                                                                                             >  4:671794/1‑107 (MQ=255)
                                                      atgTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTTGCGttttt                                                                                                                                                                                                                   <  7:998188/149‑1 (MQ=255)
                                                      atgTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGttttt                                                                                                                                                                                                                   <  5:329495/149‑1 (MQ=255)
                                                      atgTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGttttt                                                                                                                                                                                                                   >  6:899191/1‑149 (MQ=255)
                                                       tgtgAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTg                                                                                                                                                                                                                  >  6:688727/1‑149 (MQ=255)
                                                       tgtgAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTg                                                                                                                                                                                                                  >  7:274682/1‑149 (MQ=255)
                                                       tgtgAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTCTTg                                                                                                                                                                                                                  >  8:780112/1‑149 (MQ=255)
                                                          gaatgaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGt                                                                                                                                                                                                                                                         <  2:671794/107‑1 (MQ=255)
                                                          gaatgaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCCAGCAGCTg                                                                                                             <  2:629547/247‑1 (MQ=255)
                                                          gaatgaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAACAGGCTTGAGCGTGAAGCGGACAGTCATACCTGCTTTAAAGTGGCGTTTTTGTCg                                                                                                                                                                                                               >  5:316639/1‑149 (MQ=255)
                                                           aatgaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGc                                                                                                                                                                                                                         >  5:265661/1‑138 (MQ=255)
                                                           aatgaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGc                                                                                                                                                                                                                         <  6:265661/138‑1 (MQ=255)
                                                              gaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAc                                                                                                                                                                                                           <  6:634273/149‑1 (MQ=255)
                                                              gaaAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAc                                                                                                                                                                                                           >  5:42806/1‑149 (MQ=255)
                                                                aaaTTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAccc                                                                                                                                                                                                         <  7:546747/149‑1 (MQ=255)
                                                                   tttATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATa                                                                                                                                                                                                      <  5:912444/149‑1 (MQ=255)
                                                                      aTTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCt                                                                                                                                                                                                   >  6:993153/1‑149 (MQ=255)
                                                                             tAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATAtttt                                                                                                                                                                                            >  7:961664/1‑149 (MQ=255)
                                                                              aaCGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTc                                                                                                                                                                                           <  6:81984/149‑1 (MQ=255)
                                                                                 taaaaaggCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCtgt                                                                                                                                                                                        >  5:990322/7‑149 (MQ=255)
                                                                                  cATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACTTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCAAGAAATATGCCGAGCCAGGGGCAAGGAAGCGCCAGCAGCTGTTTAATGGAGTAATGTAGGGNNNAGGTGTGGTAACAGGCCTTGGTACTGaaa                                                         >  3:201460/1‑275 (MQ=255)
                                                                                   aTTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGc                                                                                                                                                                                                                         >  4:231268/1‑114 (MQ=255)
                                                                                    tttGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCCAGCAGCTGTTGAAGGGAGTACTGTAGGCAAATGGGTCCGTAAACGGCTTTCGAACTGAAAgg                                                       >  4:648214/1‑275 (MQ=255)
                                                                                     ttGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGGCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTgttgtt                                                                                                                                                                                    <  7:933102/149‑1 (MQ=255)
                                                                                     aaggCTCGAATTGGAGCCTTTTTATGACTATTCCTTAAAAAATCCCAAGTTAAGACTGTTTTTAAAGCAGGTTTGAGAGTGAATCGGACACTAATACCTGCTTTAAAGTTGCGTTTTTGTCGTTACCCATACCTATACTTTCTtttatt                                                                                                                                                                                    >  8:760314/3‑149 (MQ=255)
                                                                                      ttgCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCCAGCAGCTGTTGAATGGAGTACTGTAGGCAAATGGTGCCGTAACCGGCTTTCGTACTGaa                                                          <  1:201460/268‑1 (MQ=255)
                                                                                       ggCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGttt                                                                                                                                                                                                                     <  2:231268/114‑1 (MQ=255)
                                                                                         cTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAAc                                                                                                                                                                                <  7:801602/149‑1 (MQ=255)
                                                                                          tCCAATTGGAGCCTTTTTATGACTATTTATTATAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACt                                                                                                                                                                               >  8:925100/1‑149 (MQ=255)
                                                                                          tCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGacac                                                                                                                                                                                                                                              >  8:257743/1‑86 (MQ=255)
                                                                                          tCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACt                                                                                                                                                                               >  8:211554/1‑149 (MQ=255)
                                                                                          tCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACt                                                                                                                                                                               >  5:112659/1‑149 (MQ=255)
                                                                                           ccAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCCAGCAGCTGTTGAATGGAGTACTGTAGGCAAATGGTGCCGTAACCGGCTTTCGTACTGAAAGGTTCTGTc                                                <  2:320562/275‑1 (MQ=255)
                                                                                            cAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATAc                                                                                                                                                                                                                                        >  6:49750/1‑90 (MQ=255)
                                                                                            cAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATAc                                                                                                                                                                                                                                        <  5:49750/90‑1 (MQ=255)
                                                                                            cAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGc                                                                                                                                                                             <  7:970400/149‑1 (MQ=255)
                                                                                              aTTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCa                                                                                                                                                                           >  6:867882/1‑149 (MQ=255)
                                                                                                    gCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTg                                                                                                                                                                     <  8:411863/149‑1 (MQ=255)
                                                                                                     ccTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGa                                                                                                                                                                    <  8:401028/149‑1 (MQ=255)
                                                                                                      cTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAcc                                                                                                                                                                                                          <  2:195266/110‑1 (MQ=255)
                                                                                                      cTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAg                                                                                                                                                                   >  8:862498/1‑149 (MQ=255)
                                                                                                      cTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAg                                                                                                                                                                   >  7:672682/1‑149 (MQ=255)
                                                                                                       tttttATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGc                                                                                                                                                                  >  5:582409/1‑149 (MQ=255)
                                                                                                       tttttATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCATTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGc                                                                                                                                                                  >  8:611088/1‑149 (MQ=255)
                                                                                                        ttttATGACTATTTATTAAAATATCCTACGTCACGCCTCTGTAGAAAGCAGGCTTGAGCATGAAGCTGACACTCATACCTGCCTTAAAGTGGCGTTTTTGTCGTTTCCCCTACCTACATTTACTGTTTTTTAACGGCCAGAACTGAGCg                                                                                                                                                                 <  8:947413/149‑1 (MQ=255)
                                                                                                        ttttATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCg                                                                                                                                                                 <  5:993153/149‑1 (MQ=255)
                                                                                                        ttttATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCg                                                                                                                                                                 <  7:212577/149‑1 (MQ=255)
                                                                                                           tATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGtt                                                                                                                                                              <  6:244950/149‑1 (MQ=255)
                                                                                                               aCTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGaaa                                                                                                                                                          <  7:862498/149‑1 (MQ=255)
                                                                                                                  atttattAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGc                                                                                                                                                       <  6:735939/149‑1 (MQ=255)
                                                                                                                  atttattAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGc                                                                                                                                                       <  5:717550/149‑1 (MQ=255)
                                                                                                                       ttaAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGaa                                                                                                                                                  <  6:928476/149‑1 (MQ=255)
                                                                                                                          aaaTATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAata                                                                                                                                               <  8:69129/149‑1 (MQ=255)
                                                                                                                          aaaTATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAata                                                                                                                                               <  6:452002/149‑1 (MQ=255)
                                                                                                                          aaaTATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAata                                                                                                                                               >  8:925182/1‑149 (MQ=255)
                                                                                                                             tatCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGCACTGAGCGGTTGAAAAGCCAGAAATATGc                                                                                                                                            <  7:925100/149‑1 (MQ=255)
                                                                                                                                ccTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGa                                                                                                                                         >  5:1100910/1‑149 (MQ=255)
                                                                                                                                 cTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATAc                                                                                                                                                                                                     >  5:394015/1‑88 (MQ=255)
                                                                                                                                      tcaaGCCTGCTTTAAAAACAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGTCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCa                                                                                                                                                     <  6:478327/129‑1 (MQ=255)
                                                                                                                                      tcaaGCCTGCTTTAAAAACAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGTCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCa                                                                                                                                                     >  5:478327/3‑131 (MQ=255)
                                                                                                                                      tcaaGCCTGCTTTAAAAACAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGa                                                                                                                                                                    >  7:197367/3‑116 (MQ=255)
                                                                                                                                      tcaaGCCTGCTTTAAAAACAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGa                                                                                                                                                                    <  8:197367/114‑1 (MQ=255)
                                                                                                                                        aaGCCTGCTTTAAAAACAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCgg                                                                                                                                 >  7:506503/1‑149 (MQ=255)
                                                                                                                                         aGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTatat                                                                                                                                                                                               >  1:45466/1‑86 (MQ=255)
                                                                                                                                         aGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCCAGCAGCTGTTGAAGGGAGTACTGTAGGCAAATGGTGCCGTAACCGGCTTTAGTAATGAAAGGTTTTGTCTATCCCAACCCTTCCCTTGTATCTCACATTTAGTTGTGAAAAGcct  >  4:145171/1‑273 (MQ=255)
                                                                                                                                             tGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTc                                                                                                                                                                                           <  3:45466/86‑1 (MQ=255)
                                                                                                                                             tGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATgg                                                                                                                            >  5:741062/1‑149 (MQ=255)
                                                                                                                                             tGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCCAGCAGCTGTTGAATGGAGTACTGTAGGCAAATGGTGCCGTAACCGGCTTTCGTACTGAAAGGTTCTGTCTATCCCATCCCTTCTCTTGTATCTAACATTTAg               <  2:145171/258‑1 (MQ=255)
                                                                                                                                             tGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGACAGAAATATGCCGAGCCAGCGGCATgg                                                                                                                            >  8:344288/1‑149 (MQ=255)
                                                                                                                                               tttttAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGaa                                                                                                                          >  7:744832/1‑149 (MQ=255)
                                                                                                                                               tttttAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCACGGaa                                                                                                                          <  5:5058/149‑1 (MQ=255)
                                                                                                                                                ttttAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCCAGCAGCTGTTg                                                                                                          >  2:654446/1‑164 (MQ=255)
                                                                                                                                                 tttAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAgc                                                                                                                        <  6:385419/149‑1 (MQ=255)
                                                                                                                                                     aaGCAGGCTTGAGCGTGAAGCGGACGCTCATACCTGCTTTATAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCca                                                                                                                    >  7:347835/1‑149 (MQ=255)
                                                                                                                                                     aaGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTAccc                                                                                                                                                                                                         >  7:73604/1‑64 (MQ=255)
                                                                                                                                                      aGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCcag                                                                                                                   >  5:1021494/1‑149 (MQ=255)
                                                                                                                                                       |                                                                                                                                                                                                                                                                    
GTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCATACCTATATTTTCTGTTGTTTAACTGCCAGAACTGAGCGGTTGAAAAGCCAGAAATATGCCGAGCCAGCGGCATGGAAGCGCCAGCAGCTGTTGAATGGAGTACTGTAGGCAAATGGTGCCGTAACCGGCTTTCGTACTGAAAGGTTCTGTCTATCCCATCCCTTCTCTTGTATCTAACATTTAGTTGTGAATAGCTA  >  NC_000913/2774942‑2775353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: