| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,127,252 | A→G | 5.5% | intergenic (+27/+34) | rpmE → / ← yiiX | 50S ribosomal subunit protein L31/putative lipid binding hydrolase, DUF830 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,127,252 | 0 | A | G | 5.5% | 629.4 / 16.4 | 192 | intergenic (+27/+34) | rpmE/yiiX | 50S ribosomal subunit protein L31/putative lipid binding hydrolase, DUF830 family protein |
| Reads supporting (aligned to +/- strand): ref base A (92/87); new base G (4/7); total (98/94) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01 | |||||||||||
Only 100 of 194 total aligned reads displayed.
GAGCGACGCGGCCTTAAACCGAGGTTTTCCCATGAAAAAAGATATTCACCCGAAATACGAAGAAATTACTGCTAGCTGCTCTTGCGGTAACGTAATGAAAATCCGCTCCACCGTTGGTCATGACCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCTTTGAGCTTTGCCTGAACTTGTGGGCTGCTCAGGTCGAACTCTTTCAACTTCTGCTGCTCGCCCACGCGCATTCCCAGCGCGTTCTGGTAAACCTTCCACACCACTTCCGAA > NC_000913/4126982‑4127511 | gagCGACGCGGCCTTAAACCGAGGTTTTCCCATGAAAAAAGATATTCACCCGAAATACGAAGAAATTACTGCTAGCTGCTCTTGCGGTAACGTAATGAAAATCCGCTCCACCGTTGGTCATGACCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACgg < 2:58194/274‑1 (MQ=255)gagCGACGCGGCCTTAAACCGAGGTTTTCCCATGAAAAAAGATATTCACCCGAAATACGAAGAAATTACTGCTAGCTGCTCTTGCGGTAACGTAATGAAAATCCGCTCCACCGTTGGTCATGACCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACgg < 2:338487/274‑1 (MQ=255) ggCCTGAAACCTAGGTTTTCCCAAGTAAAAAGATATTCACCCGAAATACGAGTAAATAACTGCTGGCTGCTCTTGCTGTAACGAAAGGAAAATCCGCTCCACCGTGGGTCATGACCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTGGATCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCttttttt < 4:502512/275‑1 (MQ=255) ccATGAAAAAAGATATTCACCCGAAATACGAAGAAATTACTGCTAGCTGCTCTTGCGGTAACGTAATGAAAATCCGCTCCACCGTTGGTCATGACCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTa < 2:165419/275‑1 (MQ=255) aaaTACGAAGAAATTACTGCTAGCTGCTCTTGCGGTCACGTAATGAAAATCAGCTCTCCCGTTGGTCATGACCTCAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCCCTGGCAAACGCCGTGATGTTGCTACCAGTGGCCGTTTTGACCGCTTCAACACGAGTTTCAACATCCCTGGCAGCAACTCAGAACGACTTTACGAAACACGCGCCACACCGCCCTTTTTTTGTTCCTGAATTTTGGACGTATTACAACGAAAACAGCGGACAtt > 4:298935/1‑275 (MQ=255) ttACTGCTAGCTGCTCTTGCGGTAACGTAATGAAAATCCGCTCCACCGTTGGTCATGACCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACAGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTGCCAAGAAAACAGCGGCCATTCTTTGGCGACCg > 2:328330/1‑274 (MQ=255) aaaaTCCGCTCTACCGTTGGTCATGACCTGAACCTCGACGTGTGCAGCAAGTGTCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGATACCGGTGGCGGTGTTGACTGTTTCAACAAGCGTTGCAACATCCCCGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGAACGCACCTTTTGTTCTGCCTGAATTTTGGTCGTACTACCACGCAAACCGCGGCCATTCTTTGTCGACCGCGGTTAGTTGCGGGGTCAAC‑AAAACGGCttcgt > 4:232412/1‑272 (MQ=255) tCCGCTCCACCGTTGGTCATGACCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCACCATCCAGGGCAGCANATAAGACCGGTTTTCCGAAAAAAGCGCAGCAAGGCGCCTTTTTTGTGCCGTAATTTTGGTCGTATTACCACGAAAACAAANGCCATTGTTTGGGGACTGGGGTGACNGTTGGCG‑GATAGAAAACGGCGTggggag > 3:129584/1‑272 (MQ=255) taaagatcagccgcGACGTGTGCAGCAATTGCCACCCTTTCTTCACTGGCAAACAGCGTGTTGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACTTCCCGGGCAGCAAATAAAACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTTAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCaa < 4:558519/261‑1 (MQ=255) cTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACg > 7:129415/1‑149 (MQ=255) cTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACg > 8:242920/1‑149 (MQ=255) cTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACg > 7:315671/1‑149 (MQ=255) tGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCCAACAGCGTGATGTTGCTACCGTTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGAATTTCCGAAAAAAGCGCCGCACgg < 8:946808/149‑1 (MQ=255) gAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGgc > 8:320646/1‑149 (MQ=255) ccTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGATAAAAGCGCCGTGCGGCGCt < 6:1021441/149‑1 (MQ=255) ccTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCt < 8:368214/149‑1 (MQ=255) cTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCtt > 8:293816/1‑149 (MQ=255) cTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCtt > 8:162309/1‑149 (MQ=255) cTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGCTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCCCGGCGCtt > 6:504200/1‑149 (MQ=255) tCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCttt > 5:429645/1‑149 (MQ=255) cGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGTGCGGCGCtttt < 8:967526/149‑1 (MQ=255) cGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCttttttt < 7:418744/149‑1 (MQ=255) tgtgCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGAcc < 4:200258/201‑1 (MQ=255) gcggcgtgtgtCACCCTTTTGTTACTGGCAAACAGCGTGATGTTTCTGCCGGTGGCCGTGTTGCCCACTTCAACGAGGGTTTCAACATCCCGGGCAGCAAATAAGACCGTATTTCCGAAAAAAGCGCCGCACGTCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAAc < 4:328330/267‑1 (MQ=255) gcagcaAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAAc > 2:272028/1‑274 (MQ=255) gcagcaAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAAc > 2:202766/1‑274 (MQ=255) cagcaAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACACGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAACACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGCTTCCTCAAGGGGAATAtttctgtcccttga > 2:558519/1‑262 (MQ=255) gcaAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGa > 7:870726/1‑149 (MQ=255) gcaAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGa > 7:931169/1‑149 (MQ=255) aGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAAtt < 8:722203/149‑1 (MQ=255) gTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAAttt > 7:226352/1‑149 (MQ=255) gTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAAttt < 6:611719/149‑1 (MQ=255) ccACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTgg > 7:307699/1‑149 (MQ=255) cccGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCg > 6:574438/1‑149 (MQ=255) gTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTAt > 5:730752/1‑149 (MQ=255) tcttcACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACa > 4:175277/1‑160 (MQ=255) ttcACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTAcc > 8:183666/1‑149 (MQ=255) ttcACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTAcc > 6:184931/1‑149 (MQ=255) tcACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTAcct < 6:934829/149‑2 (MQ=255) cACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCAc < 8:931169/149‑1 (MQ=255) cACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCTTTGGGCTTTGccctgtc > 2:665260/1‑269 (MQ=255) aaaCAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACa > 6:645760/1‑149 (MQ=255) aaCAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTAc > 6:46301/1‑138 (MQ=255) cAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCg < 7:242920/149‑1 (MQ=255) ttGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCttt < 7:438662/149‑1 (MQ=255) tGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGCAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTg < 8:731013/149‑1 (MQ=255) tGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTg < 8:619255/149‑1 (MQ=255) tACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCg > 8:434057/1‑149 (MQ=255) cGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTAtt < 2:402819/117‑1 (MQ=255) gTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCgt < 8:263329/149‑1 (MQ=255) gTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGC‑TTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCgtg < 7:938260/149‑1 (MQ=255) gCCGTGTTGACCGCTTCAACGAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGa < 2:143906/142‑1 (MQ=255) tgtTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGt < 7:188302/149‑1 (MQ=255) gACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTtgtg > 7:387448/1‑149 (MQ=255) gACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCTTTGAGCTTTCCCTTAACTTGTTGGCTGCTCAGGTCGAACTCTTTCAACTTCTgctgct > 2:18379/1‑275 (MQ=255) aCCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTgg < 5:640632/149‑1 (MQ=255) ccGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTggt > 5:783576/1‑148 (MQ=255) cGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGgcg > 7:798937/1‑149 (MQ=255) cGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGgcg > 6:485283/1‑149 (MQ=255) ttCAACAAGCGTTTCAACATCCCGCGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAATTGTGGCG‑CAt < 7:948827/149‑1 (MQ=255) tCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATc < 8:676251/149‑1 (MQ=255) acaaGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGaa > 8:1048528/1‑149 (MQ=255) acaaGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGaa > 5:123059/1‑149 (MQ=255) caaGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCAAGCAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCTGTAACGTTCTTTGAGCTTTGCCTGAACTTTTGGGTTGCTCAGGTCGAAATATTTCTACTTCTGCTTCTCGCCCCCtcgc > 1:68371/1‑275 (MQ=255) cgggntttCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCNGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCtt < 4:665260/201‑1 (MQ=255) gCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGATGTGGCG‑CATCGAAAACGGCTTGTGGCTAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTTTTTGAGCTTTGCCTGAACTTGGTGGTTGCCCAGGTCGAGCTCTTTCAACTTCTTCTGCTCGCCCACGGCCAtt > 4:105238/1‑275 (MQ=255) cGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCTTTGAGATTTGCCTGAACTTGTGGGATGATCCGGTCGACCTCTTTTAACTTATGCTGCTCGCCCCCGCGCCTTc > 1:18855/1‑275 (MQ=255) gTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGTGCGGCGCttt > 6:259234/1‑62 (MQ=255) gTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGTGCGGCGCttt < 5:259234/62‑1 (MQ=38) aaCATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCGCGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTtgtg > 7:512815/1‑149 (MQ=255) aaCATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCa < 5:1036800/102‑1 (MQ=255) aaCATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCa > 6:1036800/1‑102 (MQ=255) aCATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTgg < 8:407579/149‑1 (MQ=255) aCATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTgg > 7:614704/1‑149 (MQ=255) tCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTtgtg > 4:145896/1‑124 (MQ=255) cagcaAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCTTTGAGCTTTGCCTGAACTTGTGGGCTGCTCAGGTCGAACTCTTTCAACTTCTGCTGCTCGCCCACGCGCATTCCCAGCGCGTTCTGGTaa < 2:444577/275‑1 (MQ=255) aagcaAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGATACCACCGTTTCCTAAAGCGGAATATTCTTGCCGTAACGTTCTTTGAGCTTTGCCTGAACTTGTGGGCTGCTCAGGTCGAACTCTTTCAGCTTCTGCTGCTCGCCCACGCGCATTCCCAGCGCGTTCTGGTaa < 2:232412/274‑1 (MQ=255) aTAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTcc < 5:484130/149‑1 (MQ=255) aaGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGTG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTc < 6:783576/149‑1 (MQ=255) aCCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTg < 6:868308/87‑1 (MQ=255) aCCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTg > 5:868308/1‑87 (MQ=255) ccGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGc > 6:231557/1‑149 (MQ=255) gATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCTTTGAGCTTTGCCTGAACTTGTGGGCTGCTCAGGTCGAACTCTTTCAACTTCTGTTGCTCGCCCACGCGCCTTCCCCGCGGGTTCTGGTACACCTTACccac > 1:206492/1‑271 (MQ=255) aTTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCttt > 1:43045/1‑171 (MQ=255) tttCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAat > 5:546610/1‑149 (MQ=255) tCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCTTTGAGCTTTGCCTGAACTTGTGGGCTGCTCAGGTCGAACTCTTTCAACTTCTGCTGCTCGCCCa < 4:404410/230‑1 (MQ=255) ccGAAAAAAGCGCCGCACGGCGCTTTTTTTGTNCCTGAATTNTGGGCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCTTTGAGc < 3:43045/171‑1 (MQ=255) ccGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATAtt < 5:684283/149‑1 (MQ=255) ctaaaaaaGGGCCGAACGGGGCTTTTTTTGTGCCTGAAATTTGGTGGTATTACCACGTAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCTTTGAGCTTTGCCTGAACTTGTGGGCTGCTCAGGTCGAACTCTTTCAACTTCTGCTGCTCGCCCACGCGCATTCCCAGCGCGTTCTGGTAAACCTTCCACACCACTTCCGaa < 4:608715/273‑1 (MQ=255) cGAACAAAGGGCCGCACGGCGCTTTTTTTTTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCTTTGAGCTTTGCCTGAACTTGTGGGCTGCTCAGGTCGAACTCTTTCAACTTCTGCTGCTCGCCCACGCGCATTCCCAGCGCGTTCTGGTAAACCTTCCACACCACTTCCGaa < 4:202766/275‑1 (MQ=255) cGAAAAAAGCGCCGCACGTCGCTTTTTTTGTGCCTGAATTTTTGTCGTATTACCACGAAAACAGCGGCCATTCTTTGTCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCTAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCTTTGAGCTTTGCCTGAACTTGTGGGCTGCTCAGGTCGAACTCTTTCAACTTCTGCTGCTCGCCCACGCGCATTCCCAGCGCGTTCTGGTAAACCTTCCACACCACTTCCGaa < 1:129584/275‑1 (MQ=255) cGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTc < 8:854141/149‑1 (MQ=255) gAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCt > 6:712305/1‑149 (MQ=255) aaaaaaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTc < 6:923418/44‑1 (MQ=255) aaaaaaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCtt < 7:724981/149‑1 (MQ=255) aaaaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGc > 5:373123/1‑149 (MQ=255) aaaGCGCCGTGCGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGcc > 7:336268/1‑149 (MQ=255) aaaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGcc > 7:1020051/1‑149 (MQ=255) gccgcACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAAc > 5:219100/1‑149 (MQ=255) cgcgcgGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGt > 5:950029/1‑149 (MQ=255) | GAGCGACGCGGCCTTAAACCGAGGTTTTCCCATGAAAAAAGATATTCACCCGAAATACGAAGAAATTACTGCTAGCTGCTCTTGCGGTAACGTAATGAAAATCCGCTCCACCGTTGGTCATGACCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCG‑CATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCTTTGAGCTTTGCCTGAACTTGTGGGCTGCTCAGGTCGAACTCTTTCAACTTCTGCTGCTCGCCCACGCGCATTCCCAGCGCGTTCTGGTAAACCTTCCACACCACTTCCGAA > NC_000913/4126982‑4127511 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |