Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 481,228 C→A 6.8% intergenic (‑520/+26) tomB ← / ← acrB Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913481,2280CA 6.8% 552.4 / 21.3 163intergenic (‑520/+26)tomB/acrBHha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (76/76);  new base A (6/5);  total (82/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01

Only 100 of 165 total aligned reads displayed.

AGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGACGTAAACGCATCCG  >  NC_000913/481013‑481499
                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                               
aGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTa                                                                                                                                                                                                                                                        >  4:9617/1‑241 (MQ=255)
    atgaatAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCaa                                                                                                                                                                                                                                                    <  2:9617/241‑1 (MQ=255)
                                                                      tAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATc                                                                                                                                                                                                                                                      >  2:286784/1‑173 (MQ=255)
                                                                       cgcgcTACTTAATATAAATTAACTAACACTCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCCCTTACGCgg                                                                                                                                                                                                                                                                             <  8:931033/148‑1 (MQ=255)
                                                                       aGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCgg                                                                                                                                                                                                                                                                             >  6:309835/1‑149 (MQ=255)
                                                                         cgcTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGcc                                                                                                                                                                                                                                                                           <  6:625382/149‑1 (MQ=255)
                                                                             aCTTAATATAAATTAACTATCAATCGGTATTATTTATGTTTTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCtttt                                                                                                                                                                                                                                                                       <  5:52659/149‑3 (MQ=255)
                                                                              cTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGt                                                                                                                                                                                                                                                                      <  8:714809/149‑1 (MQ=255)
                                                                                 aaTATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAt                                                                                                                                                                                                                                                                   <  5:601081/149‑1 (MQ=255)
                                                                                 aaTATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAt                                                                                                                                                                                                                                                                   <  8:652497/149‑1 (MQ=255)
                                                                                 aaTATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAt                                                                                                                                                                                                                                                                   <  6:400826/149‑1 (MQ=255)
                                                                                   tataAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTTATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTa                                                                                                                                                                                                                                                                 >  6:160969/1‑149 (MQ=255)
                                                                                   tataAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTa                                                                                                                                                                                                                                                                 >  8:1080560/1‑149 (MQ=255)
                                                                                      aaaTTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTCACGTTTCGGGCACCa                                                                                                                                 >  4:555987/1‑274 (MQ=255)
                                                                                            actaacAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTa                                                                                                                                                                                                                                                        <  8:121076/149‑1 (MQ=255)
                                                                                             ctaacAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTAt                                                                                                                                                                                                                                                       <  8:1071132/149‑1 (MQ=255)
                                                                                              taacAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATc                                                                                                                                                                                                                                                      >  5:206331/1‑149 (MQ=255)
                                                                                              taacAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATc                                                                                                                                                                                                                                                      >  8:175014/1‑149 (MQ=255)
                                                                                               aacAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCa                                                                                                                                                                                                                                                     >  6:21486/1‑149 (MQ=255)
                                                                                                 cAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAat                                                                                                                                                                                                                                                   <  5:457080/149‑1 (MQ=255)
                                                                                                    tCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatgat                                                                                                                                                                                                                                                >  5:774374/1‑149 (MQ=255)
                                                                                                    tCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatgat                                                                                                                                                                                                                                                <  7:368364/149‑1 (MQ=255)
                                                                                                       gTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATgatgat                                                                                                                                                                                                                                             <  8:864987/149‑1 (MQ=255)
                                                                                                          ttttttATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGACAGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGa                                                                                                                                                                                                                                          >  8:357397/1‑149 (MQ=255)
                                                                                                           tttatATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGAc                                                                                                                                                                                                                                         <  7:1065342/145‑1 (MQ=255)
                                                                                                               tATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTa                                                                                                                                                                                                                                     <  7:102923/149‑1 (MQ=255)
                                                                                                                  gAATTTTATCCGTTGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCTGCCTTAGTATTTACACGTTGTATCAATGATGATCGACAGTATgg                                                                                                                                                                                                                                  <  8:922503/149‑1 (MQ=255)
                                                                                                                      tttATCCGTTGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtgt                                                                                                                                                                                                                              <  5:390848/149‑1 (MQ=255)
                                                                                                                       ttATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtgtg                                                                                                                                                                                                                             >  5:232008/1‑149 (MQ=255)
                                                                                                                        tATCCGTGGTTAATACTGTTTTTCGTATGAGATCCAGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTGACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGc                                                                                                                                                                                                                            <  5:318532/149‑1 (MQ=255)
                                                                                                                          tCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCtttttt                                                                                                                                                                                                                                                                     >  7:44558/1‑102 (MQ=255)
                                                                                                                          tCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCtttttt                                                                                                                                                                                                                                                                     <  8:44558/106‑5 (MQ=255)
                                                                                                                             gTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCtttt                                                                                                                                                                                                                                                                       >  8:284346/1‑99 (MQ=255)
                                                                                                                             gTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCtttt                                                                                                                                                                                                                                                                       <  7:284346/101‑3 (MQ=255)
                                                                                                                               ggTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAt                                                                                                                                                                                                                                                                   >  3:142128/1‑103 (MQ=255)
                                                                                                                               ggTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCg                                                                                        >  1:120185/1‑274 (MQ=255)
                                                                                                                                  tAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCtt                                                                                                                                                                                                                  <  5:863757/149‑1 (MQ=255)
                                                                                                                                     tACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttt                                                                                                                                                                                                                                                                      <  6:917010/94‑4 (MQ=255)
                                                                                                                                          gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTac                                                                                                                                                                                                                                                                >  4:143034/1‑95 (MQ=255)
                                                                                                                                          gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATg                                                                                                                                                                                                                                   >  4:490578/1‑124 (MQ=255)
                                                                                                                                          gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCAt                                                                                                                       >  4:195929/1‑232 (MQ=255)
                                                                                                                                            tttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGt                                                                                                                                                                                                                                                            <  6:425190/97‑1 (MQ=255)
                                                                                                                                            tttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCg                                                                                                                                                                                                        <  7:651691/149‑1 (MQ=255)
                                                                                                                                              tCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtg                                                                                                                                                                                                                               <  2:490578/124‑1 (MQ=255)
                                                                                                                                              tCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTaca                                                                                                                   <  2:195929/232‑1 (MQ=255)
                                                                                                                                              tCGTATGAGATCCTGAGTTGGTGGTCCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGt                                                                                                                                                                                                                                                            <  2:143034/95‑1 (MQ=255)
                                                                                                                                                 tATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTaa                                                                                                                                                                                                   >  8:767457/1‑149 (MQ=255)
                                                                                                                                                  aTGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTaaa                                                                                                                                                                                                  <  6:892737/149‑1 (MQ=255)
                                                                                                                                                   tGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAg                                                                                                                                                                                                 >  6:87816/1‑149 (MQ=255)
                                                                                                                                                    gagaTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTTCGGTCACCATCCCGCCCATTACACCGGTACCTACTTCGTTCTGCGCGCCGGAACCAGCCCCAGTAcctaaa                                                                   >  4:132573/1‑269 (MQ=255)
                                                                                                                                                       aTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc                                                                                                                                                                                             >  8:1045358/1‑149 (MQ=255)
                                                                                                                                                         ccTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaag                                                                                                                                                             >  2:521135/1‑179 (MQ=255)
                                                                                                                                                          cTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCaa                                                                                                                                                                                                                                                    <  6:86549/91‑1 (MQ=255)
                                                                                                                                                          cTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggc                                                                                                                                                                                          >  7:324670/1‑149 (MQ=255)
                                                                                                                                                             aGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAagaaga                                                                                                                                                         <  4:521135/179‑1 (MQ=255)
                                                                                                                                                             aGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCTCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCa                                                         <  2:132573/275‑1 (MQ=255)
                                                                                                                                                                  gtggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAaccacc                                                                                                                                                                                  >  8:1043059/1‑149 (MQ=255)
                                                                                                                                                                  gtggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAAc                                                     >  4:502236/1‑274 (MQ=255)
                                                                                                                                                                     gtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCt                                                                                                                                                                                                                                >  5:888780/1‑100 (MQ=255)
                                                                                                                                                                     gtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca                                                                                                                                                                               >  6:823755/1‑149 (MQ=255)
                                                                                                                                                                         aaTTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGa                                                                                                                                                                           >  8:192860/1‑149 (MQ=255)
                                                                                                                                                                         aaTTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGa                                                                                                                                                                           >  6:753799/1‑149 (MQ=255)
                                                                                                                                                                          aTTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTc                                                                                                                                                                                                             <  5:500091/114‑1 (MQ=255)
                                                                                                                                                                          aTTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTc                                                                                                                                                                                                             >  6:500091/1‑114 (MQ=255)
                                                                                                                                                                          aTTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATAGCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGaa                                                                                                                                                                          <  8:758053/149‑1 (MQ=255)
                                                                                                                                                                           ttACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAAt                                                                                                                                                                         >  7:89627/1‑149 (MQ=255)
                                                                                                                                                                             aCTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATAc                                                                                                                                                                       <  6:206331/149‑1 (MQ=255)
                                                                                                                                                                               tCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaa                                                                                                                                                              >  2:752830/1‑156 (MQ=255)
                                                                                                                                                                                ccTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTa                                                                                                                                                                                                                                                        >  3:22158/1‑65 (MQ=255)
                                                                                                                                                                                 cTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGa                                                                                                                                                                   >  7:769270/1‑149 (MQ=255)
                                                                                                                                                                                  ttAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGc                                                                                                                                                                                                                                 >  8:684110/1‑86 (MQ=255)
                                                                                                                                                                                  ttAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGc                                                                                                                                                                                                                                 <  7:684110/86‑1 (MQ=255)
                                                                                                                                                                                  ttAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGaa                                                                                                                                                                  <  7:182866/149‑1 (MQ=255)
                                                                                                                                                                                   tAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAAc                                                                                                                                                                 >  5:693342/1‑149 (MQ=255)
                                                                                                                                                                                   tAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGaagaag                                                                                                                                                          <  4:752830/156‑1 (MQ=255)
                                                                                                                                                                                   gataagagacaGGTTATGAATAAAAAAGGCCGCTTACGCGGACTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCa                                    <  1:155081/264‑1 (MQ=255)
                                                                                                                                                                                    aaTGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCaa                                                                                                                                                                                                                                                    <  1:22158/65‑1 (MQ=255)
                                                                                                                                                                                    aaTGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCAt                                                                                                                                                                                                               >  1:141726/1‑102 (MQ=255)
                                                                                                                                                                                      tGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAAGACCGGAACgaa                                                                                                                                                              >  6:593150/1‑149 (MQ=255)
                                                                                                                                                                                        ttCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCtt                                                                                                                                                                                                           <  3:141726/102‑1 (MQ=255)
                                                                                                                                                                                           gTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACaa                                                                                                                                                                              >  4:447528/1‑128 (MQ=255)
                                                                                                                                                                                           gTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAagaaga                                                                                                                                                         >  6:905049/1‑149 (MQ=255)
                                                                                                                                                                                           gTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCCGTACTGATAACCCGCGGCCTAACGCCGAGGATAAACGCCAGCCGGGtca                           >  2:538522/1‑275 (MQ=255)
                                                                                                                                                                                             aGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGAtt                                                                                                                                                       <  7:175014/149‑1 (MQ=255)
                                                                                                                                                                                             aGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGAtt                                                                                                                                                       <  6:810273/149‑1 (MQ=255)
                                                                                                                                                                                               gTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGaa                                                                                                                                                                          <  2:447528/128‑1 (MQ=255)
                                                                                                                                                                                               gTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGc                                                                                                                                                     >  7:597728/1‑149 (MQ=255)
                                                                                                                                                                                                  aTGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAg                                                                                                                                                  <  8:597728/149‑1 (MQ=255)
                                                                                                                                                                                                   tGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGAt                    >  1:152495/1‑274 (MQ=255)
                                                                                                                                                                                                     aatcactaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGTTATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTAc                                                                                                                                               >  5:932190/8‑149 (MQ=255)
                                                                                                                                                                                                      aTAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCg                                                                                                                                                                                                        >  7:716614/1‑91 (MQ=255)
                                                                                                                                                                                                         aaaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt                                                                                                                                           <  7:1043059/149‑1 (MQ=255)
                                                                                                                                                                                                         aaaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt                                                                                                                                           >  6:800076/1‑149 (MQ=255)
                                                                                                                                                                                                          ctaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg                                                                                                                                          >  8:944560/3‑149 (MQ=255)
                                                                                                                                                                                                                  gCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa                                                                                                                     >  2:584835/1‑162 (MQ=255)
                                                                                                                                                                                                                    ttACGCGGCCTTAGTGGTTAAACGGTGGACAACGGCTGATCGACAGGAGGGGTGGGCTCGAAATCCTCATTCTTGCGGCTAAGGGGGCGGCGAAGCATCACAAATAATACCGGAACGAACAAGATTGCAAGTACCGTCGGGGTCACCATCGCGCTCAGTACACCGGTACCTACTGCGTGCTGAGCGCGGGAACCAGCACCAGGACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGACGTAAACGCATccg  <  4:164546/275‑1 (MQ=255)
                                                                                                                                                                                                                    ttACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTAGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCAt                                                                                                                                >  5:945467/1‑149 (MQ=255)
                                                                                                                                                                                                                      aCGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATcc                                                                                                                              >  5:69959/1‑149 (MQ=255)
                                                                                                                                                                                                                      aCGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACAcc                                                                                                                 <  4:584835/162‑1 (MQ=255)
                                                                                                                                                                                                                      aCGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATaa                                                                  >  2:680935/1‑209 (MQ=255)
                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                               
AGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGACGTAAACGCATCCG  >  NC_000913/481013‑481499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: