Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,608,704 | A→G | 5.0% | S107P (TCG→CCG) | yneE ← | bestrophin family putative inner membrane protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,608,704 | 0 | A | G | 5.0% | 460.7 / 9.3 | 140 | S107P (TCG→CCG) | yneE | bestrophin family putative inner membrane protein |
Reads supporting (aligned to +/- strand): ref base A (65/67); new base G (2/5); total (68/72) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.43e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 |
Only 100 of 141 total aligned reads displayed.
TTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTG > NC_000913/1608457‑1608974 | ttAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTACCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCAGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTcgcg > 1:127327/1‑275 (MQ=255) aTCGTTGAGGCTTATAAACAGGATATCTAAAAGCTGTACATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCAAGTACACGCTGAAGATCTTCAGTCTTTAGCTAATGAGCCAGCACTTCCGCCTGTGGCTGCTTGCGTAATGTCATCCCTAAACAGTGGGCGAAAGCGATTCGCAGCCTGGCAAATCCCCTTACACTTCCCGAATCCGGCATTGTCGTTTCTACCTccaacagtacac > 4:111715/1‑264 (MQ=255) aGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTCCCTAGCGCAGTAACGACCGTGAGGCCATCATCaa > 4:145712/1‑275 (MQ=255) aaaGCTGTCCATTGCGTCGCTGTACCGCCAACCACTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTAGACGATGAAGTTCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGTAATGTCGTTTTTACCTCGCGCACTAATGACCGTGATTGATTCATCAACTTCCCCCa > 4:289497/1‑274 (MQ=255) aGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGt > 2:83813/1‑249 (MQ=255) ccATTTCGGCGCTGAACCGCTAACCATTCTCCCATTATTAACAATGTACGGTTAGCTCGAGAGTTCGAAGCCAGTACACGCTGAAGATCCTCAGTCTGTATATAATGATCCAGCTCTTCCTCTTGTGGCTGTATGCGTAATGTCATGCGTAAGCAGTGGGCGAAAGCGATTTGCAGCCTCGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTcg < 4:381945/275‑1 (MQ=255) aTTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTcgcg < 2:55542/275‑1 (MQ=255) attaACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGCATCCGGCAATGTCGTTTTTACCTCGCCCAGTAACGACGTTGAGGCAATCAACAACTGCCCCCAAAGTTTGCGTCCATAAAGGTAA‑GGGGGTATCCCAGCAATATTACg > 4:635223/1‑275 (MQ=255) gATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCaa > 4:425395/1‑275 (MQ=255) aGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGt > 2:113073/1‑190 (MQ=255) gctgggaggTTCGAAGCCAGTACACGTGGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACATCCGCCTGTTGTTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAatggc < 2:635223/267‑1 (MQ=255) ggAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATc > 8:157354/1‑149 (MQ=255) ggAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATc > 6:295029/1‑149 (MQ=255) ggAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAgg < 4:113073/190‑1 (MQ=255) ttCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaa < 7:987268/149‑1 (MQ=255) ttCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCaa > 7:725559/1‑149 (MQ=255) cGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaagt < 5:643810/149‑3 (MQ=255) gAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGt > 8:490331/1‑149 (MQ=255) gAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGt > 8:44870/1‑149 (MQ=255) gAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGt < 7:490331/149‑1 (MQ=255) aaGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAgg < 4:480620/180‑1 (MQ=255) aGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGt > 2:174578/1‑175 (MQ=255) gCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGt < 7:775358/149‑1 (MQ=255) ccAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGtt < 7:692833/149‑1 (MQ=255) cAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGttt > 7:272527/1‑149 (MQ=255) aGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAgg < 4:174578/175‑1 (MQ=255) acacGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTAc < 6:749293/149‑1 (MQ=255) cacGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCGGTAACGACCGTGAGGCAATCATCAACGGCCCCCAAAGTTTTCGCGCTTCAACGGAACGGGCGTA‑CACGGCCTCATTACGAAAACCAAGAAAAATGGAGAGAGCGACACCgagacagacg > 4:89043/1‑269 (MQ=255) acGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGttt < 1:49492/143‑1 (MQ=255) cTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAAc < 5:68207/149‑1 (MQ=255) cTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAAc < 7:821404/149‑1 (MQ=255) ttCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaagt > 6:862165/1‑123 (MQ=255) ttCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaagt < 5:862165/125‑3 (MQ=255) aGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGAcc < 5:803294/149‑1 (MQ=255) gTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTACACGTAACGGGCGTA‑CCCGGCATCATTACCAAAACCAAGAAAAATGGAGATGGCGACACCCAGAATGCTTGACGGTGCggggaggaa > 4:268111/1‑270 (MQ=255) tCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGt < 6:737206/149‑1 (MQ=255) gaTAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCa > 8:239776/1‑149 (MQ=255) aTGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCTAGGGTGAATTTGATGCCCa > 2:269293/1‑275 (MQ=255) aTGAGCCAGCACTTCCGCCTGGGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAatca < 8:1070780/149‑1 (MQ=255) ccAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAAc > 5:560139/1‑149 (MQ=255) ccAGCACTGCCGCCGGTGGCTGTTTGCGTAATGTCATGCGTAAACAGGGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGAt < 1:56050/273‑1 (MQ=255) cAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACt > 5:722068/1‑149 (MQ=255) aGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTg > 8:495693/1‑149 (MQ=255) aCTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGTAGTAACGACCGTGAGGCAATCATCAACTGccc > 6:939221/1‑149 (MQ=255) aCTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGccc < 6:133244/149‑1 (MQ=255) cTTCCGCCTGGGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTAc < 2:190632/275‑1 (MQ=255) gCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGGTCGGGGGTGGATTTGCTGCCTAAAGACGGTTACCAggg > 4:190632/1‑275 (MQ=255) tgtgGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGttt < 6:1103827/149‑1 (MQ=255) gCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTcgc < 7:157354/149‑1 (MQ=255) gTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGt > 4:734467/1‑113 (MQ=255) cGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAAc < 6:112672/149‑1 (MQ=255) cGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTCGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAAc < 8:112462/149‑1 (MQ=255) gTAATGTCATGCGTAAACAGTGTGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAAc < 2:268111/274‑1 (MQ=255) tCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCATTGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACgg < 7:1020744/149‑1 (MQ=255) tCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACgg > 6:548620/1‑149 (MQ=255) tCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACgg > 8:112970/1‑149 (MQ=255) tGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAg > 8:668789/1‑101 (MQ=255) tGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAg < 7:668789/101‑1 (MQ=255) tAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCTGGCAGTAACGACCGTGAGGCAATCCCCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGGCACCGAGAATGCAGAACGGTGCGAGGGTGAATTTGAGTCCCAGATGAGGGTACCAGGGCAGCATAACAATAACAGCCACAGaaaaaa > 4:647898/1‑273 (MQ=255) ggCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGt > 2:735251/1‑85 (MQ=255) ggCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCattat > 5:181363/1‑149 (MQ=255) ggCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGc > 4:457203/1‑191 (MQ=255) ggCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGGGCGAGGGTGAATTTGATGCCCAGACGCGTATACCAGGGCAGCATGAAAAAAACAGCGATAGAAAAGAGAAAAtcaa > 1:158292/1‑272 (MQ=255) cGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCAttatta < 6:73220/149‑1 (MQ=255) gAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTAc > 8:290355/1‑149 (MQ=255) gAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTAc < 5:558411/149‑1 (MQ=255) aaaGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAgg < 4:735251/85‑1 (MQ=255) aaaGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGaa < 2:457203/191‑1 (MQ=255) aaGGGATGTTCAGCCGGGCAAACTCCCTTACAATGGCCGAAGCCGGCAATGTCGTTTTTACCTCGCGCAGTAAAGACCGTGAGGCAATCATCAACGGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCTGCATTATTACGAAATCCAAGAAAAATGGCGATGGCGACACCGGGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGagtagt < 4:269293/275‑1 (MQ=255) aGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGagtagt < 2:425395/274‑1 (MQ=255) aGCGATTTGCAGCCGAGAAAACGCCCGGACACTTGCAGAAGCCGGAAATGTCGTTTTTAACACGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCAAAAAGTTTTCGCGCTTCAACGGAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGagtagt < 1:80095/274‑1 (MQ=255) cGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGaaaa < 7:36231/149‑1 (MQ=255) gATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAAc < 8:725559/149‑1 (MQ=255) tGCAGCCGGGCAAACTCCCTTCCACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAgcg < 2:289497/272‑1 (MQ=255) tGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAg < 8:652644/149‑1 (MQ=255) aGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGaaa > 8:38747/1‑149 (MQ=255) gCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGaaaa > 5:640084/1‑149 (MQ=255) tgtcAAACTCCCTTCCCCTTGCCGAATCCTCCACTGTCGTTTTTACCTCGCGCATTAACGACCGTGAGGCAATCATCATCTTCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGAAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATc < 1:2365/272‑1 (MQ=255) gggCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGt < 8:124742/92‑1 (MQ=255) gggCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGt > 7:124742/1‑92 (MQ=255) gggCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAatg > 8:501851/1‑149 (MQ=255) aggCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGTGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATc < 2:136521/274‑1 (MQ=255) ggCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAatgg < 5:1038564/149‑1 (MQ=255) ggCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAatgg < 7:1064701/149‑1 (MQ=255) aCTCCCTTAAACTTGACGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCATCTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAATACCAAGAAAAatggcgttg < 6:1017468/149‑1 (MQ=255) tCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAatggcgatggc > 8:1005001/1‑149 (MQ=255) tCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAatggcgatggc < 7:38747/149‑1 (MQ=255) tCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAatggcgatggc < 8:272527/149‑1 (MQ=255) tACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGacac > 6:1024545/1‑149 (MQ=255) gacattGCCGGATTCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAg > 6:84617/5‑128 (MQ=255) gacattGCCGGATTCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAg < 5:84617/124‑1 (MQ=255) acTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCga < 7:495693/149‑1 (MQ=255) ttGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑ccc > 2:705096/1‑103 (MQ=255) ttGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCACGAAAAATGGCGATGGCGACACCgaga > 7:475361/1‑149 (MQ=255) ttGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCACCAACTGCCCCAAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCgaga > 6:164898/1‑149 (MQ=255) tGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGaa < 5:682440/149‑1 (MQ=255) gCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCAGGAAAATAACAGCGATAGAAAAGCAGACATTGAGTAGTAAGCGCGAGGATAACTTGGATAATACTGAGcctgc > 4:324208/1‑272 (MQ=255) cGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCGCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGc > 8:738714/1‑149 (MQ=255) cGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGc < 6:77621/149‑1 (MQ=255) gAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCt < 6:48038/149‑1 (MQ=255) | TTAAGCCGATCGTTGAGGCTAATAAACAGGATATCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTA‑CCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTG > NC_000913/1608457‑1608974 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |