BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,020,838	T→C	8.7%	V251A (GTG→GCG)	fliM →	flagellar motor switching and energizing component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,020,838	0	T	C	8.7%	416.7 / 23.8	140	V251A (GTG→GCG)	fliM	flagellar motor switching and energizing component
Reads supporting (aligned to +/- strand): ref base T (78/47); new base C (5/7); total (83/57)
Fisher's exact test for biased strand distribution p-value = 2.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-03

Only 100 of 142 total aligned reads displayed.

TGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCG  >  NC_000913/2020572‑2021108

tGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTACGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTAGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTCACCCGCCGCTGGACAACTCGCGTAATGAATATCAGAACTGGCGCGATAACCTGGTGCGCCAg                                                                                                                                                                                                                                                                          >  2:407995/1‑274 (MQ=255)

    ggCCATAGCGACGCCTGGAAGGCGAGTAATCCGCTGGAAGTTGAGGACGTGCGGTCGGAAATGCAGGTGAAATTTACAAATATCACCACCCCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGAATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTgca                                                                                                                                                                                                                                                                     <  2:19982/275‑1 (MQ=255)

     gCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTTCGACAGGTgcag                                                                                                                                                                                                                                                                    >  2:167249/1‑275 (MQ=255)

         taGGGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATTTCACAACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTgca                                                                                                                                                                                                                                                                     <  2:149886/270‑1 (MQ=255)

                                                  aCGTGCGTTCGGAAATGCAGGTGAACTTTACCAATATCACCACCTCGCCGCACGACATTGTGGTTAACACACCGTTCCATATGGAGATTGGCAACCTGACCGGCCAATTTAATCGCTGCCTGCCATTCAGCATGATCGCGCCGCTACGGGAATTGTTGGGTAACCCGCCGCTGGAAAACTCGCGTCATCACGCACAGAACTGGCGCGATAACCTGGTGCTCCAGATGCAACACTCCCAGCTGGAGCTGGTCGCCAACTTCGCCGattttccgccc                                                                                                                                                                                                                       >  4:561107/1‑266 (MQ=255)

                                                                                     atCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGa                                                                                                                                                                                   >  2:103400/1‑275 (MQ=255)

                                                                                          caccTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGCAAANTCGCGTAATGAAGATCAGAACTGGCGCGATAAACTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCNAACTTTTCCGATATCTCGCTCCCCCTTCCGNNACTTTTAACACAGACAccccccgcactcc                                                                                                                                                                               >  3:89484/1‑266 (MQ=255)

                                                                                               cGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTTCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCAGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCTACGTCCTACCg                                                                                                                                                                         >  2:620641/1‑275 (MQ=255)

                                                                                               cGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACagctggagctg                                                                                                                                                                                                                                                  >  2:210375/1‑203 (MQ=255)

                                                                                               cGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTAGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCg                                                                                                                                                                         >  2:363659/1‑275 (MQ=255)

                                                                                                                      acGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGt                                                                                                                                                                                                                                                                                 >  8:393514/1‑149 (MQ=255)

                                                                                                                       cGCCTTTCCATGTGGAGATTGGCAACCTGACCGGCGAAGTTAATATCTGCCTGCCAGTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGAGCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGcatcat                                                                                                                                                  <  1:154976/274‑1 (MQ=255)

                                                                                                                               cATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGAGCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGt                                                                                                                                                                                                                                                                        >  8:436871/1‑149 (MQ=255)

                                                                                                                                aggtgGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTg                                                                                                                                                                                                                                                                       <  8:896174/147‑1 (MQ=255)

                                                                                                                                 tgtgGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTgc                                                                                                                                                                                                                                                                      >  5:112109/1‑149 (MQ=255)

                                                                                                                                  gtgGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGCGCACCAGGt                                                                                                                                                                                                                                                                        >  6:228243/1‑146 (MQ=255)

                                                                                                                                   tgGAGATTGGCAACCTGACCGGGGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGCGCACCAGGttatc                                                                                                                                                                                                                                                                    <  8:617273/149‑5 (MQ=255)

                                                                                                                                   tgGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGCGCACCAGGttatc                                                                                                                                                                                                                                                                    <  6:954903/149‑5 (MQ=255)

                                                                                                                                    ggAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAACAACCCGATCGCATCATCGCCCATGTTGACg                                                                                                                                    >  2:243283/1‑275 (MQ=255)

                                                                                                                                      agaTTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTAGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACCGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCTACGTCCTTCCGAAAGAAAACCCCGATACCATCCTCCCCCaatgttccgg                                                                                                                                   >  4:453171/1‑265 (MQ=255)

                                                                                                                                         ttGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTca                                                                                                                                                                                                                                                              <  7:703497/149‑1 (MQ=255)

                                                                                                                                          tGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTc                                                                                                                                                                                                                                                               <  4:199312/147‑1 (MQ=255)

                                                                                                                                           ggCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTcaca                                                                                                                                                                                                                                                            >  8:647068/1‑149 (MQ=255)

                                                                                                                                           ggCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTcaca                                                                                                                                                                                                                                                            <  5:632381/149‑1 (MQ=255)

                                                                                                                                            gCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACag                                                                                                                                                                                                                                                           >  5:434064/1‑149 (MQ=255)

                                                                                                                                             cAACCTGAACGACGAATTGAATATCGGCCACCCGATCAGCATGATCGGGCCGCTAAGGGAATTGTTGGTTAACCCGCCGCTGGAAGGCTCGCGTAATGAAGATCAGAACTGGCACGTTAACCTGGGGCGCCAGATGCAGCATTCACAGCTGGAGCTGGTAGCCAAC‑GTGCCGATAACTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGt                                                                                                                          <  4:211934/275‑1 (MQ=255)

                                                                                                                                                 cTGACCGTCTAATGTAACATCTACCTGCCATTCGGCGTGATCCAGCCGGTACGGGGATTGTTGGTTAACCCGCCTCTGGAAAACACGCGTAATGAAGGTCAGAGCTGGCGCGATAACCTGGTGCGCCAGGTGCAACATTCACAGCTGGCGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGACGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTg                                                                                                                         <  1:196737/273‑1 (MQ=255)

                                                                                                                                                    aCCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGCTTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGACCATGTTGACGGCGTCCCGGtcgtga                                                                                                                     >  1:5157/1‑269 (MQ=255)

                                                                                                                                                      cGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGCGCACCAGGttatc                                                                                                                                                                                                                                                                    >  6:1007896/1‑126 (MQ=255)

                                                                                                                                                      cGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGCGCACCAGGttatc                                                                                                                                                                                                                                                                    <  5:1007896/130‑5 (MQ=255)

                                                                                                                                                       ggCTAATTTAAGATCTGCCTGCCATGAAGCATGAGCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAAGGAAGATCAGAACTGGCGCGAGAACCTGGGGCGCCAGGTGCAGCATTCACAGCTGGAGCTTATCGCCAACTTTGCCGATATCTCGCTACGCCTGTAGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACca                                                                                                                  <  1:74835/274‑1 (MQ=255)

                                                                                                                                                        gCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACagctggagctg                                                                                                                                                                                                                                                  >  6:99560/1‑146 (MQ=255)

                                                                                                                                                        gCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACagctggagctg                                                                                                                                                                                                                                                  <  5:99560/146‑1 (MQ=255)

                                                                                                                                                           aaTTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGcc                                                                                                                                                                                                                                            >  8:189289/1‑149 (MQ=255)

                                                                                                                                                             tttAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTgca                                                                                                                                                                                                                                                                     <  6:745613/122‑1 (MQ=255)

                                                                                                                                                             tttAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTgca                                                                                                                                                                                                                                                                     >  5:745613/1‑122 (MQ=255)

                                                                                                                                                             tttAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCaa                                                                                                                                                                                                                                          >  5:79851/1‑149 (MQ=255)

                                                                                                                                                             tttAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGCGCACCAGGttat                                                                                                                                                                                                                                                                     <  7:516198/122‑4 (MQ=255)

                                                                                                                                                             tttAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGCGCACCAGGttat                                                                                                                                                                                                                                                                     >  8:516198/1‑119 (MQ=255)

                                                                                                                                                              ttAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAAc                                                                                                                                                                                                                                         >  8:906308/1‑149 (MQ=255)

                                                                                                                                                              ttAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAAc                                                                                                                                                                                                                                         >  8:339116/1‑149 (MQ=255)

                                                                                                                                                                aaTATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACtt                                                                                                                                                                                                                                       >  5:664383/1‑149 (MQ=255)

                                                                                                                                                                    tCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGcc                                                                                                                                                                                                                                   >  7:194106/1‑149 (MQ=255)

                                                                                                                                                                       gcctgccATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGat                                                                                                                                                                                                                                <  7:574823/149‑1 (MQ=255)

                                                                                                                                                                       gccggcaATTCAGCATGATCGAGCGGTTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCt                                                                                                  <  2:561107/274‑1 (MQ=255)

                                                                                                                                                                        cctgccATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCa                                                                                                >  2:573618/1‑275 (MQ=255)

                                                                                                                                                                              aTTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGc                                                                                                                                                                                                                         >  8:31935/1‑149 (MQ=255)

                                                                                                                                                                                  aGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACg                                                                                                                                                                                                                     >  8:601912/1‑149 (MQ=255)

                                                                                                                                                                                    cATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGcc                                                                                                                                                                                                                   >  7:714447/1‑149 (MQ=255)

                                                                                                                                                                                     aTGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCt                                                                                                                                                                                                                  >  6:211613/1‑149 (MQ=255)

                                                                                                                                                                                     aTGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGCGCACCAGGttatc                                                                                                                                                                                                                                                                    >  8:78864/1‑95 (MQ=255)

                                                                                                                                                                                        aTCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTc                                                                                                                                                                                                               <  8:257368/149‑1 (MQ=255)

                                                                                                                                                                                        aTCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTc                                                                                                                                                                                                               >  8:150163/1‑149 (MQ=255)

                                                                                                                                                                                           gAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTGGCTACGCCTGTCGCa                                                                                                                                                                                                            >  7:831227/1‑149 (MQ=255)

                                                                                                                                                                                           gAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCa                                                                                                                                                                                                            >  6:1034369/1‑149 (MQ=255)

                                                                                                                                                                                            aGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCCTTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCCGCTTTTAAAACTGA‑ACCCCGGCGCCGTCCTGCCCATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCCTCCCGGTGCTGGCCAGCCAGTATGGCACCCTCACCGGTCAGTATGCGttctg                                                                             >  1:79418/1‑271 (MQ=255)

                                                                                                                                                                                            aGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAACGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACgg                                                                            >  2:292710/1‑275 (MQ=255)

                                                                                                                                                                                              ccGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATNCCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAAACCGAGCGCNAACTCACCCCTGTTTACGGCGCCCCGGTGCTCACCAACCCGTATGNGNCCCCCCACGGCCAGTCTCCGtttccttt                                                                          >  3:59765/1‑269 (MQ=255)

                                                                                                                                                                                               cGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTg                                                                                                                                                                                                                                                                       >  7:333003/1‑86 (MQ=255)

                                                                                                                                                                                               cGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGAtt                                                                                                                                                                                                        <  5:303727/149‑1 (MQ=255)

                                                                                                                                                                                                     gggAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTaaaa                                                                                                                                                                                                  <  7:321910/149‑1 (MQ=255)

                                                                                                                                                                                                      gaaatgtatgGTTAACACGCCACGGGAAAACCCGCGGAATGAAGACCAGAACTGGCTCGGTAACCTGTGGCGCCAGATGCAGCAGTCCAAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTAGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGACGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCGGACAGTATGGCACCCGAAACGGTCAGTATGCGTTACGGATAGAACAt                                                                   <  4:407995/266‑1 (MQ=255)

                                                                                                                                                                                                         aTTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGa                                                                                                                                                                                              >  7:874606/1‑149 (MQ=255)

                                                                                                                                                                                                          ttgttgGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGa‑a                                                                                                                                                                                            >  6:610912/1‑149 (MQ=255)

                                                                                                                                                                                                                gTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGTTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCgg                                                                                                                                                                                      >  7:25628/1‑149 (MQ=255)

                                                                                                                                                                                                                  tAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTCTTGCACCCTCAACGGTCAGTATGCGTTCAGGATAGAACATTTGATTAACCCGa                                                      >  2:636275/1‑275 (MQ=255)

                                                                                                                                                                                                                  tAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCACGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCaa                                                                                               >  2:341123/1‑234 (MQ=255)

                                                                                                                                                                                                                      ccgccgCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCACGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGt                                                                                           <  4:341123/234‑1 (MQ=255)

                                                                                                                                                                                                                       cgccgcTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTc                                                                                                                                                                               >  7:378154/1‑149 (MQ=255)

                                                                                                                                                                                                                               gAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGAt                                                                                                                                                                       <  8:660479/149‑1 (MQ=255)

                                                                                                                                                                                                                                aaaaCTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATa                                                                                                                                                                      >  6:410187/1‑149 (MQ=255)

                                                                                                                                                                                                                                    cTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGaaa                                                                                                                                                                  >  7:68694/1‑149 (MQ=255)

                                                                                                                                                                                                                                    cTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGt                                                                                           >  2:448803/1‑220 (MQ=255)

                                                                                                                                                                                                                                     tggcgTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGaaaa                                                                                                                                                                 >  8:741863/3‑149 (MQ=255)

                                                                                                                                                                                                                                       gcgTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAg                                  >  4:251350/1‑274 (MQ=255)

                                                                                                                                                                                                                                        cgTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGt                                                                                       <  4:448803/220‑1 (MQ=255)

                                                                                                                                                                                                                                         gTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGa                                <  4:103400/274‑1 (MQ=255)

                                                                                                                                                                                                                                           aaTGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGa                                                                                                                                                           >  7:1046484/1‑149 (MQ=255)

                                                                                                                                                                                                                                           aaTGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGaa                               <  2:251350/273‑1 (MQ=255)

                                                                                                                                                                                                                                                aGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGca                                                                                                                                                      <  7:484060/149‑1 (MQ=255)

                                                                                                                                                                                                                                                 gATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGcat                                                                                                                                                     >  5:255659/1‑149 (MQ=255)

                                                                                                                                                                                                                                                  aTCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGCTAGAAAAACCCGATCGcatc                                                                                                                                                    >  5:225818/1‑149 (MQ=255)

                                                                                                                                                                                                                                                    cAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGcatcat                                                                                                                                                  >  7:4595/1‑149 (MQ=255)

                                                                                                                                                                                                                                                    cAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGcatcat                                                                                                                                                  <  6:457621/149‑1 (MQ=255)

                                                                                                                                                                                                                                                     aGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCatcatc                                                                                                                                                 <  8:831227/149‑1 (MQ=255)

                                                                                                                                                                                                                                                         cTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAAGGGGTg                >  2:570415/1‑274 (MQ=255)

                                                                                                                                                                                                                                                            gcgcGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCAGGAGCTGGTCGCCAACTTTGCCGATATCGCGCTACTCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATg                                                                                                                                          >  8:485962/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                gATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGa                                                                                                                                      >  5:836915/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                 tgcaCCTGGCGCACCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGAc                                                                                                                                     <  5:399284/146‑1 (MQ=255)

                                                                                                                                                                                                                                                                 tgcaCCTGGCGCACCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGAc                                                                                                                                     >  6:399284/4‑149 (MQ=255)

                                                                                                                                                                                                                                                                 aTAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGAc                                                                                                                                     <  6:395165/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                 aTAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAAt                     >  4:641100/1‑261 (MQ=255)

                                                                                                                                                                                                                                                                    aCCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGc                                                                                                                                  >  7:552366/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                     ccTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGt                 <  2:641100/261‑1 (MQ=255)

                                                                                                                                                                                                                                                                      cTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGt                                                                                                                                >  8:213073/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                      cTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGt                                                                                                                                <  7:601912/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                      cTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCCACGGTCAGTATGCGTTACGGCTAGAACCTTTGATTAACCCGATTTTGAATTCTCTGAAAGAGGAACAGCCCAAATAAGTGACATGAATAATccc  >  2:622948/1‑274 (MQ=255)

                                                                                                                                                                                                                                                                       tGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGc                                                                                                                                  <  5:857650/146‑1 (MQ=255)

                                                                                                                                                                                                                                                                       tGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGc                                                                                                                                  >  6:857650/1‑146 (MQ=255)

                                                                                                                                                                                                                                                                       tGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTc                                                                                                                               >  5:886707/1‑149 (MQ=255)

TGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGA‑ACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTCCCGGTGCTGACCAGTCAGTATGGCACCCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGAGTGACATGAATAATCCG  >  NC_000913/2020572‑2021108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`