Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,704,304 A→G 6.3% intergenic (‑241/+31) rnc ← / ← lepB RNase III/leader peptidase (signal peptidase I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,704,3040AG 6.3% 623.8 / 20.8 191intergenic (‑241/+31)rnc/lepBRNase III/leader peptidase (signal peptidase I)
Reads supporting (aligned to +/- strand):  ref base A (97/82);  new base G (3/9);  total (100/91)
Fisher's exact test for biased strand distribution p-value = 7.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.58e-01

Only 100 of 194 total aligned reads displayed.

CCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGTCCCGGAGGAACAATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCC  >  NC_000913/2704040‑2704564
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                    
                                      taaatGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGAc                                                                                                                                                                                                                                                            <  4:240540/237‑1 (MQ=255)
                                              tCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTTAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTa                                                                                                                                                                                                              >  2:65496/1‑275 (MQ=255)
                                                   agagacagacacGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAgcg                                                                                                                                                                                                          <  2:146128/266‑1 (MQ=255)
                                                          cacaCGAAACAGACCTGAGATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCa                                                                                                                                                                                                   <  2:562084/274‑1 (MQ=255)
                                                                   cAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCAtt                                                                                                                                                                                         <  4:65496/275‑1 (MQ=255)
                                                                                    gTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTTTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGa                                                                                                                                                                                                                                                 <  4:21553/202‑1 (MQ=255)
                                                                                    gTTCTGCTAACTGCTTCGCTGCAGTTTAAGTTTACCGGTATATGGGGACCAACGCTGGTTCGTGTGCCGTGGCAAACTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATc                                                                                                                                                                         <  2:18715/274‑1 (MQ=255)
                                                                                         gCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACt                                                                                                                                                                                                                <  2:369703/230‑1 (MQ=255)
                                                                                          cTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCAATTCGCCTTCTTGCTTATCGAAGCta                                                                                                                                                                  >  1:53818/1‑274 (MQ=255)
                                                                                           tAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACg                                                                                                                                                                                                                                                  <  2:344954/194‑1 (MQ=255)
                                                                                            aaCTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTc                                                                                                                                                                                                <  2:43045/243‑1 (MQ=255)
                                                                                                              tcagtttACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACCACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGGGACTTAAGCACAGACCAGTCGGCCATTCGCCTTATTTGTTAACGAAGCCCAACCAAATTCCCGTTGccc                                                                                                                                               >  4:394535/1‑274 (MQ=255)
                                                                                                                      ccGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATa                                                                                                                                                                                                                                                                    >  5:174205/1‑149 (MQ=255)
                                                                                                                       cGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATaa                                                                                                                                                                                                                                                                   >  5:659643/1‑149 (MQ=255)
                                                                                                                           atatGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCTAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGc                                                                                                                                                                                                                                                               <  6:22171/149‑1 (MQ=255)
                                                                                                                           atatGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGc                                                                                                                                                                                                                                                               <  7:470552/149‑1 (MQ=255)
                                                                                                                           atatGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGc                                                                                                                                                                                                                                                               <  6:134185/149‑1 (MQ=255)
                                                                                                                               ggggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACa                                                                                                                                                                                                                                                           <  5:667560/149‑1 (MQ=255)
                                                                                                                                gggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACCCAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACaa                                                                                                                                                                                                                                                          <  8:707477/149‑1 (MQ=255)
                                                                                                                                 ggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAAc                                                                                                                                                                                                                                                         <  7:228694/149‑1 (MQ=255)
                                                                                                                                 ggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAAc                                                                                                                                                                                                                                                         <  6:944463/149‑1 (MQ=255)
                                                                                                                                 ggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTACCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCgcccac                                                                                                                                                                                                                                                         <  6:401092/149‑6 (MQ=255)
                                                                                                                                  gACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAACg                                                                                                                                                                                                                                                        <  7:899116/149‑1 (MQ=255)
                                                                                                                                  gACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACg                                                                                                                                                                                                                                                        >  7:584850/1‑149 (MQ=255)
                                                                                                                                   aCCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGt                                                                                                                                                                                                                                                       <  5:797897/149‑1 (MQ=255)
                                                                                                                                      aaCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAACGTGaa                                                                                                                                                                                                                                                    >  8:766843/1‑148 (MQ=255)
                                                                                                                                      aaCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAACGTGaa                                                                                                                                                                                                                                                    <  7:766843/148‑1 (MQ=255)
                                                                                                                                       aCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAAc                                                                                                                                                                                                                                                   <  5:76180/149‑1 (MQ=255)
                                                                                                                                       aCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGAGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGACACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAAc                                                                                                                                                                                                                                                   <  7:708283/149‑1 (MQ=255)
                                                                                                                                          cTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGaa                                                                                                                                                                                                                                                <  6:174205/149‑1 (MQ=255)
                                                                                                                                          cTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGaa                                                                                                                                                                                                                                                <  5:560926/149‑1 (MQ=255)
                                                                                                                                           tGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAg                                                                                                                                                                                                                                               >  5:339146/1‑149 (MQ=255)
                                                                                                                                                       cGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAAt                                                                                                                                                                                                                                   <  7:1080938/149‑1 (MQ=255)
                                                                                                                                                        gTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATg                                                                                                                                                                                                                                  >  8:598216/1‑149 (MQ=255)
                                                                                                                                                         tGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATgg                                                                                                                                                                                                                                 >  7:650043/1‑149 (MQ=255)
                                                                                                                                                           gCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGTGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACACCGTGAACGAATATGGCTATTAATGGAt                                                                                                                                                                                                                               >  6:1064457/1‑149 (MQ=255)
                                                                                                                                                             aaCCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTaa                                                                                                                                                                                                                                    <  2:397018/142‑1 (MQ=255)
                                                                                                                                                             aaCCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATgc                                                                                                                                                                                                                             >  5:966227/1‑149 (MQ=255)
                                                                                                                                                             aaCCTGGAGGTGCCAACCTTAAACTTCAGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATgc                                                                                                                                                                                                                             >  7:722193/1‑149 (MQ=255)
                                                                                                                                                                cTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATgccgc                                                                                                                                                                                                                          >  5:361184/1‑149 (MQ=255)
                                                                                                                                                                   gAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCaa                                                                                                                                                                                                                       >  8:902627/1‑149 (MQ=255)
                                                                                                                                                                   gAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCaa                                                                                                                                                                                                                       >  8:207212/1‑149 (MQ=255)
                                                                                                                                                                      gTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGc                                                                                                                                                                                                                    >  5:455160/1‑149 (MQ=255)
                                                                                                                                                                       tGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCAACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCg                                                                                                                                                                                                                   >  6:658552/1‑149 (MQ=255)
                                                                                                                                                                         ccAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGAc                                                                                                                                                                                                                 >  8:763339/1‑149 (MQ=255)
                                                                                                                                                                         ccAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGAc                                                                                                                                                                                                                 >  8:167201/1‑149 (MQ=255)
                                                                                                                                                                          cAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACt                                                                                                                                                                                                                <  7:763339/149‑1 (MQ=255)
                                                                                                                                                                              cTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAg                                                                                                                                                                                                            >  8:277534/1‑149 (MQ=255)
                                                                                                                                                                              cTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAg                                                                                                                                                                                                            >  7:950283/1‑149 (MQ=255)
                                                                                                                                                                                 aaaCTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAACGCCCCAGTAACGGCCGTCGCCGCTGTTGTCGCGGtttgcc                                                                           >  4:552271/1‑271 (MQ=255)
                                                                                                                                                                                  aaCTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAAc                                                                                                                                                                                                                                                         >  5:777111/1‑100 (MQ=255)
                                                                                                                                                                                  aaCTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAAc                                                                                                                                                                                                                                                         <  6:777111/100‑1 (MQ=255)
                                                                                                                                                                                  aaCTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCa                                                                                                                                                                                                        <  7:207212/149‑1 (MQ=255)
                                                                                                                                                                                    cTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGa                                                                                                                                                                                                      <  8:584850/149‑1 (MQ=255)
                                                                                                                                                                                       cgtgggAACAGTCTTTACACAACTACTGAGTCAGCCGTTAGCAACGTGTAACTTATTTAATAATAAGTTCCAGTCGTCGGAATAATGTCGACAACATTTACGAATCTGTCTATTATACGATTCTGCCAATGCGACTGAGGCACAGACTATACGGCTATTCGCCTTCTTGCTTATCGAAGCACATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCCCAAAGCCACAGTAACGGCTGTCCGCGCTGTTGTCGTGGTTGCAGcccct                                                                      >  4:187090/4‑272 (MQ=255)
                                                                                                                                                                                       cGGGGGCATATTCTATACACAACGACGGGGGCTGTCGTTAGCCGCGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCANTGCGACTTAAGCGCAGACCAGTCGGCCCTTCTCCTTCTTGCTTATCGACGCTCATCAAGATAGCCGTTGNCCGACCGACCATATTCGCTTCCGGCCCCCACCCCCCCTAACGGCTNCNCCCCCTCTTGTCGGGGttattcccacg                                                                      >  3:57580/1‑265 (MQ=255)
                                                                                                                                                                                       cGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATc                                                                                                                                                                         >  2:697022/1‑175 (MQ=255)
                                                                                                                                                                                          gggAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGc                                                                                                                                                                                                                    <  6:970074/129‑1 (MQ=255)
                                                                                                                                                                                          gggAATATGCTAAACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATg                                                                  <  2:242362/275‑1 (MQ=255)
                                                                                                                                                                                           ggAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAg                                                                                                                                                                     <  4:697022/175‑1 (MQ=255)
                                                                                                                                                                                           ggAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCcg                                                                                               <  4:365023/245‑1 (MQ=255)
                                                                                                                                                                                            gAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACtt                                                                                                                                                                                                               <  2:194054/132‑1 (MQ=255)
                                                                                                                                                                                                 ttCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCAtt                                                                                                                                                                                         >  5:95288/1‑149 (MQ=255)
                                                                                                                                                                                                         acaACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACg                                                                                                                                                                                                                                                  >  2:751859/1‑84 (MQ=255)
                                                                                                                                                                                                         acaACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCcttct                                                                                                                                                                                 >  5:233894/1‑149 (MQ=255)
                                                                                                                                                                                                          caACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTACTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCcttctt                                                                                                                                                                                <  5:722720/149‑1 (MQ=255)
                                                                                                                                                                                                           aaCGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTg                                                                                                                                                                               >  8:334404/1‑149 (MQ=255)
                                                                                                                                                                                                           aaCGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACaa                                                                                                                    >  1:144542/1‑208 (MQ=255)
                                                                                                                                                                                                           aaCGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGTCCCggagg                                                 >  2:606677/1‑275 (MQ=255)
                                                                                                                                                                                                               acgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAGCGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATc                                                                                                                                                               <  2:716455/161‑1 (MQ=255)
                                                                                                                                                                                                                cgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCACGACCGACCAGATTCGCTTCCGGCACAAAGCCCAAGTAACGGCTGTCAGCGCTGGTGCCGGGGTCTGCGCCCCACATTGTGTATGGTCCCGGAGGAACag                                            >  4:73025/1‑274 (MQ=255)
                                                                                                                                                                                                                 gggggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATc                                                                                                                                                                         >  7:302688/1‑149 (MQ=255)
                                                                                                                                                                                                                    ggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGTTGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGaa                                                                                                                                                                      <  5:945321/149‑1 (MQ=255)
                                                                                                                                                                                                                          cGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCTCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGTTTATCGAATCTCATCCAGATAGCCGTTGCCCGACCGACCTGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTTGCCCTTCATGAAGTATTTTCCTGGCGGAACAATCTAGGTTGc                                  >  1:140943/1‑275 (MQ=255)
                                                                                                                                                                                                                                ccACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGAt                                                                                                                                                          <  7:1023942/149‑1 (MQ=255)
                                                                                                                                                                                                                                 cACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATa                                                                                                                                                         >  6:123153/1‑149 (MQ=255)
                                                                                                                                                                                                                                    gggAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAAAGTGAAGGAAGAGGGCTATTAATGGATGCGGCCAATGCGACTTAAGCGCAGACCAGTCGGCAATTCGCCTTCTTGCGTATCGAAGCTCATCCAGATAGcc                                                                                                                                                      >  6:892295/1‑149 (MQ=255)
                                                                                                                                                                                                                                      gagaTTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACCTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGt                                                                                                                                                    <  8:356018/149‑1 (MQ=255)
                                                                                                                                                                                                                                            tATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGTCCCGGAGGAACAATCCAGGTTGCCAGTTGTTGCCCTGgctg                <  2:223522/275‑1 (MQ=255)
                                                                                                                                                                                                                                              tctcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgac                                                                                                                                            >  7:612509/1‑149 (MQ=255)
                                                                                                                                                                                                                                                tcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgaccg                                                                                                                                          >  7:375774/1‑149 (MQ=255)
                                                                                                                                                                                                                                                tcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgaccg                                                                                                                                          >  5:524034/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  ataaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCcgaccgac                                                                                                                                        >  5:191739/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  ataaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCcgaccgac                                                                                                                                        <  5:647574/149‑1 (MQ=255)
                                                                                                                                                                                                                                                   taaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCgaccgacc                                                                                                                                       <  8:43876/149‑1 (MQ=255)
                                                                                                                                                                                                                                                    aaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCa                                                                                                                                      >  6:733393/1‑149 (MQ=255)
                                                                                                                                                                                                                                                       taatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGAt                                                                                                                                   <  5:1064457/149‑1 (MQ=255)
                                                                                                                                                                                                                                                         atTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTc                                                                                                                                 <  6:785727/149‑1 (MQ=255)
                                                                                                                                                                                                                                                          ttacagTTGGCGCCAAATCGGCGAAAACGTGAACGATGATGGCTATTAATGGATGCCCCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGCTAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGTCCCGGAGGAACAATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATcc  <  4:254303/270‑1 (MQ=255)
                                                                                                                                                                                                                                                          ttCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCg                                                                                                                                >  6:595031/1‑149 (MQ=255)
                                                                                                                                                                                                                                                          ttCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGTCCCGGAGGAACAATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATcc  <  4:435918/275‑1 (MQ=255)
                                                                                                                                                                                                                                                             aCGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGc                                                                                                                                                                    <  6:289564/110‑1 (MQ=255)
                                                                                                                                                                                                                                                             aCGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGc                                                                                                                                                                    >  5:289564/1‑110 (MQ=255)
                                                                                                                                                                                                                                                             aCGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCtt                                                                                                                             >  8:914075/1‑149 (MQ=255)
                                                                                                                                                                                                                                                              cGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTc                                                                                                                            <  5:123153/149‑1 (MQ=255)
                                                                                                                                                                                                                                                               gTTGTCGCCATAACGGCGACAACGTGAACGAAGCTGACTATTAATGGATGCCACCAATGCGACTTAGGCGCAAACCGGTTGGCCATTCTTCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCTGATTCGCTTcc                                                                                                                           >  8:569782/1‑149 (MQ=255)
                                                                                                                                                                                                                                                               gTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGc                                                                                                                                                                                             >  1:174978/1‑83 (MQ=255)
                                                                                                                                                                                                                                                               gTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTcc                                                                                                                           <  6:524034/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                     gCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGcaca                                                                                                                     >  8:1047776/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                      ccATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACaa                                                                                                                    >  6:570357/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                    
CCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGTCCCGGAGGAACAATCCAGGTTGCCAGTTGTTGCCCTGGCTGCTGGTAATACATCC  >  NC_000913/2704040‑2704564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: