Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,330,402 | Δ1 bp | 10.0% | intergenic (+21/+15) | sohB → / ← yciN | inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,330,402 | 0 | A | . | 10.0% | 419.2 / 39.2 | 130 | intergenic (+21/+15) | sohB/yciN | inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN |
Reads supporting (aligned to +/- strand): ref base A (63/53); new base . (3/10); total (66/64) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.16e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 |
Only 100 of 130 total aligned reads displayed.
TGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACG > NC_000913/1330255‑1330546 | tGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGTAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGAc < 2:346609/149‑1 (MQ=255) gAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGAcc < 3:100376/149‑1 (MQ=255) aGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTc > 4:869532/1‑149 (MQ=255) gTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGt < 2:13417/149‑1 (MQ=255) gTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGt < 1:74222/149‑1 (MQ=255) gTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGt > 2:885546/1‑149 (MQ=255) gTCAATGTACGCGACATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGt > 3:82154/1‑149 (MQ=255) tCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGtt < 1:429315/149‑1 (MQ=255) cAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGttt < 4:539496/149‑1 (MQ=255) aaTGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGtttt < 1:297443/149‑1 (MQ=255) aaTGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGtttt > 4:619581/1‑149 (MQ=255) aaTGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGtttt < 1:159524/149‑1 (MQ=255) aaTGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGtttt < 2:622679/149‑1 (MQ=255) aaTGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGtttt < 1:772757/149‑1 (MQ=255) gTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGCAAAACGCGAGGTCTTAG‑CCTCGCGtttgtct < 1:97603/149‑5 (MQ=38) tACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGtttgtctt > 2:297706/1‑144 (MQ=255) aCGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGTTTGTCttt < 1:297706/149‑1 (MQ=255) cGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTGAAAGACAAACGCGAGGTCTTAG‑CCTCGCGtttgtctttt < 4:894421/149‑8 (MQ=255) gCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTaa < 1:484103/149‑1 (MQ=255) atGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAAc < 3:99733/149‑1 (MQ=255) cAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAg > 1:858654/1‑149 (MQ=255) aGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAga < 1:59507/149‑1 (MQ=255) gCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAgat > 4:365340/1‑149 (MQ=255) gTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAgatga > 1:784502/1‑149 (MQ=255) aaaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGata > 3:787905/1‑149 (MQ=255) aCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAtt < 1:616696/149‑1 (MQ=255) gACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAtttt > 1:312310/1‑149 (MQ=255) tCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGATG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt < 2:936919/149‑1 (MQ=255) tCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGtttgtcttt < 3:294288/126‑7 (MQ=38) tCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGTTTGTCttt > 4:294288/1‑126 (MQ=38) tCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt > 2:995688/1‑149 (MQ=255) tCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt < 4:788628/149‑1 (MQ=255) tCAGTGACCGATTCGCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt > 2:1001802/1‑149 (MQ=255) aTTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGa > 2:462996/1‑149 (MQ=255) aTTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGa > 2:351046/1‑149 (MQ=255) aTTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGa > 4:289568/1‑149 (MQ=255) aTTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTGTCTTTAATCAACCAGATGATATTTTTCTGa > 3:437835/1‑149 (MQ=255) tGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGaaa > 2:453854/1‑149 (MQ=255) cGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGcac > 2:172757/1‑149 (MQ=255) cGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGcac > 1:87401/1‑149 (MQ=255) gATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGcaca < 1:172757/149‑1 (MQ=255) aTTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACAt > 2:669935/1‑149 (MQ=255) tCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATgg < 2:724483/149‑1 (MQ=255) aCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAAc > 1:107245/1‑119 (MQ=255) aCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAAc < 2:107245/119‑1 (MQ=255) aCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGc > 2:188348/1‑149 (MQ=255) cAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGgt < 1:157622/149‑1 (MQ=255) gcgcGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGgtgt > 2:203591/1‑149 (MQ=255) gcgGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGttt < 3:423758/149‑1 (MQ=255) gcgGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGttt > 4:529814/1‑149 (MQ=255) gcgGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGttt < 3:146284/149‑1 (MQ=255) gCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGaa < 1:188348/149‑1 (MQ=255) gCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGaa < 2:667123/149‑1 (MQ=255) gagCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACatat < 4:763522/149‑1 (MQ=255) agCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATAtt > 3:334320/1‑149 (MQ=255) gcgcCGATCGATTGTTACTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTa > 3:465147/1‑149 (MQ=255) gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTc > 1:485474/1‑113 (MQ=255) gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTc < 2:485474/113‑1 (MQ=255) atcgatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACAcc > 3:785520/1‑149 (MQ=255) atTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAtttt < 1:575458/103‑1 (MQ=255) atTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAtttt < 3:879140/103‑1 (MQ=255) atTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAtttt > 4:879140/1‑103 (MQ=255) atTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAtttt > 2:575458/1‑103 (MQ=255) cTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCtttt < 1:889229/149‑1 (MQ=255) tACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCtttt < 1:840930/148‑1 (MQ=255) tACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCtttt > 2:840930/1‑148 (MQ=255) tACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTg > 4:219910/1‑149 (MQ=255) tACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTg > 2:533454/1‑149 (MQ=255) tACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTg < 2:202417/149‑1 (MQ=255) tACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTg < 3:179972/149‑1 (MQ=255) aCGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGGCCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTgg < 1:698563/149‑1 (MQ=255) tggtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGt > 2:703502/1‑149 (MQ=255) gtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTg > 2:329275/1‑149 (MQ=255) gggTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCtt < 2:87401/149‑1 (MQ=255) cAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTc < 2:884704/149‑1 (MQ=255) cAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGATTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTc < 1:325472/149‑1 (MQ=255) aaaGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCAt < 1:246623/149‑1 (MQ=255) aaaGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGAAGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCAt > 3:249445/1‑149 (MQ=255) aGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTTATCAACCCGATGATACTTTTCTGAAAGCACATGGGTCACGTGTTTGGACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATTCTTATTCATCt > 2:799156/1‑149 (MQ=255) aTTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaa > 3:556083/1‑149 (MQ=255) aTTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaa > 3:966174/1‑149 (MQ=255) ttGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa > 1:15678/1‑149 (MQ=255) ttGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa < 1:252785/149‑1 (MQ=255) ttGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa < 3:835492/149‑1 (MQ=255) ttGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa < 1:351046/149‑1 (MQ=255) tAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTc > 4:102112/1‑149 (MQ=255) tAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTc > 4:72346/1‑149 (MQ=255) tAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTc < 2:168446/149‑1 (MQ=255) aaaaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg > 3:496637/1‑149 (MQ=255) aaaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAAc > 3:359923/1‑88 (MQ=255) aaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGcc > 1:924350/1‑149 (MQ=255) aGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATc < 2:583976/133‑1 (MQ=255) aaaCGCGAGGTCTTAG‑CCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTaa > 3:355527/1‑149 (MQ=255) aaaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGt > 1:431272/1‑124 (MQ=255) aaaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTaa > 4:949637/1‑149 (MQ=255) aaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTaaa < 4:73481/149‑1 (MQ=255) gcgAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATAc < 3:949637/149‑1 (MQ=255) gAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCa < 1:275155/149‑1 (MQ=255) tAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACg > 1:178455/1‑149 (MQ=255) tAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGCTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACg > 4:731899/1‑149 (MQ=255) | TGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACG > NC_000913/1330255‑1330546 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |