Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,725,630 Δ1 bp 86.4% coding (117/1299 nt) kgtP ← alpha‑ketoglutarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,725,6300A.86.4% 361.8 / 66.3 110coding (117/1299 nt)kgtPalpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base A (6/8);  new base . (47/48);  total (54/56)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01

Only 100 of 110 total aligned reads displayed.

CTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGA  >  NC_000913/2725488‑2725773
                                                                                                                                              |                                                                                                                                               
cTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGaaa                                                                                                                                           >  3:631377/1‑149 (MQ=255)
cTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAAt                                                                                                                                          <  4:814349/149‑1 (MQ=255)
    gCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGaa                                                                                                                                      <  3:478942/149‑1 (MQ=255)
     cGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGaa                                                                                                                                      <  3:601632/149‑1 (MQ=255)
      ggCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAAcc                                                                                                                                    >  3:419319/1‑149 (MQ=255)
         cAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACt                                                                                                                                 >  4:110159/1‑149 (MQ=255)
             tAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGa                                                                                                                              >  3:665137/1‑149 (MQ=255)
             tAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTCCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGAc                                                                                                                             <  4:911032/149‑1 (MQ=255)
               gCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCa                                                                                                                           <  3:27381/149‑1 (MQ=255)
               gCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGAACa                                                                                                                           <  3:810478/149‑1 (MQ=255)
                ccAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAg                                                                                                                          >  3:263430/1‑149 (MQ=255)
                 cAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGa                                                                                                                         <  4:781285/149‑1 (MQ=255)
                 cAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGa                                                                                                                         <  1:355174/149‑1 (MQ=255)
                 cAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGa                                                                                                                         >  1:68153/1‑149 (MQ=255)
                  aaCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGa                                                                                                                         <  4:631377/149‑1 (MQ=255)
                  aaCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAAt                                                                                                                                          <  1:117815/131‑1 (MQ=255)
                  aaCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAAt                                                                                                                                          >  2:117815/1‑131 (MQ=255)
                  aaCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTc                                                                                                                                >  1:1085379/1‑141 (MQ=255)
                  aaCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTc                                                                                                                                <  2:1085379/141‑1 (MQ=255)
                  aaCCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGAt                                                                                                                        >  3:648661/1‑149 (MQ=255)
                     cGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTAc                                                                                                                     >  1:14602/1‑149 (MQ=255)
                        cTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTg                                                                                                                  >  3:1023897/1‑149 (MQ=255)
                          aTTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGaa                                                                                                                                      <  4:955167/127‑1 (MQ=255)
                          aTTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGaa                                                                                                                                      >  3:955167/1‑127 (MQ=255)
                             gggCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAagag                                                                                                             >  1:935988/1‑149 (MQ=255)
                              ggCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAaga                                                                                                              <  1:1039926/147‑1 (MQ=255)
                               gcgcATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGc                                                                                                           <  2:920417/149‑1 (MQ=255)
                               gcgcATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGc                                                                                                           <  2:364844/149‑1 (MQ=255)
                                 gcATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGccc                                                                                                         >  4:901748/1‑149 (MQ=255)
                                  cATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGccc                                                                                                         <  2:98493/149‑1 (MQ=255)
                                        gAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAAt                                                                                                  >  1:11181/1‑149 (MQ=255)
                                        gAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGAGGGGGGCAAAGTAGAGGGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAAt                                                                                                  >  4:347514/1‑149 (MQ=255)
                                           tCCCGCAGCAAAAACACCTGCTGTTTGGAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAGGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGc                                                                                               <  2:721763/149‑1 (MQ=255)
                                            cccGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGcc                                                                                              <  4:31613/149‑1 (MQ=255)
                                             ccGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGccc                                                                                             >  4:132364/1‑149 (MQ=255)
                                               gcagcaAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCaa                                                                                           >  3:806032/1‑149 (MQ=255)
                                                   caAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgc                                                                                       >  4:139481/1‑149 (MQ=255)
                                                    aaaaaCACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgcg                                                                                      >  3:240702/1‑149 (MQ=255)
                                                    aaaaaCACCTGCTGTTTGTAGTAGTTGAGTCGTCGAGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgcg                                                                                      <  3:740018/149‑1 (MQ=255)
                                                     aaaaCACCTGCTGTTTTTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgcgg                                                                                     <  2:6144/149‑1 (MQ=255)
                                                     aaaaCACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgcg                                                                                      >  3:941891/1‑149 (MQ=255)
                                                      aaaCACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCACATgcggc                                                                                    >  4:532926/1‑149 (MQ=255)
                                                        acacCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcga                                                                                  >  1:826369/1‑149 (MQ=255)
                                                           ccTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGaa                                                                                                                                            <  3:140863/88‑1 (MQ=255)
                                                           ccTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcgacga                                                                               <  2:600026/149‑1 (MQ=255)
                                                           ccTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcgacga                                                                               >  2:247725/1‑149 (MQ=255)
                                                           ccTGCTGTACGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGaa                                                                                                                                            >  4:140863/1‑88 (MQ=255)
                                                             tgctgTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGt                                                                             >  4:138173/1‑149 (MQ=255)
                                                             tgctgTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGt                                                                             >  4:57476/1‑149 (MQ=255)
                                                              gctgTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGt                                                                             <  1:659021/149‑1 (MQ=255)
                                                              gctgTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTa                                                                            >  2:811951/1‑149 (MQ=255)
                                                               ctgTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTAt                                                                           <  1:829114/149‑1 (MQ=255)
                                                                 gTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAATAAGATGTGGGGAAAGTAGACTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTAACa                                                                         <  2:1088091/149‑1 (MQ=255)
                                                                 gTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCa                                                                         <  2:267902/149‑1 (MQ=255)
                                                                     gtagtaGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCactac                                                                     <  1:1061396/149‑1 (MQ=255)
                                                                     gtagtaGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCactac                                                                     <  3:110159/149‑1 (MQ=255)
                                                                       agtagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACtt                                                                   <  3:142496/149‑1 (MQ=255)
                                                                        gtagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTAGtt                                                                   >  3:718336/1‑149 (MQ=255)
                                                                        gtagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTg                                                                  <  1:294954/149‑1 (MQ=255)
                                                                         tagtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGt                                                                 >  2:251093/1‑149 (MQ=255)
                                                                           gtTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCa                                                               <  2:14602/149‑1 (MQ=255)
                                                                                 tcgtcgTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGccccc                                                                                                       >  1:246427/1‑104 (MQ=255)
                                                                                 tcgtcgTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGccccc                                                                                                       <  2:246427/104‑1 (MQ=255)
                                                                                 tcgtcgTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGc                                                         <  3:139481/149‑1 (MQ=255)
                                                                                  cgtcgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCTAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCt                                                        <  4:1023897/149‑1 (MQ=255)
                                                                                    acgtGTTCCCGGAAGGGAAGAAGAGGTGGGCAAAGTAGAGTGAACAGAACGAGTAGATTAGAAATCGAACCACTCGACTAGATGACCTGAATAGTCCCCCACAATCGCCCAAATGCGGCGACGAGTATCAATATGTGTCCGTTGGCCGt                                                      >  4:48600/2‑149 (MQ=255)
                                                                                     cgtGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTc                                                     <  4:263430/149‑1 (MQ=255)
                                                                                      gtgtTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCt                                                    >  2:1002832/1‑149 (MQ=255)
                                                                                        gtTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGc                                                  <  2:73225/149‑1 (MQ=255)
                                                                                           cccGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGt                                               <  4:1012950/149‑1 (MQ=255)
                                                                                             cGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGtt                                              <  4:718336/149‑1 (MQ=255)
                                                                                             cGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTa                                             >  4:44084/1‑149 (MQ=255)
                                                                                               gaagggaagAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACa                                           <  4:541967/149‑1 (MQ=255)
                                                                                                aagggaagAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAg                                          >  2:12687/1‑149 (MQ=255)
                                                                                                aagggaagAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAg                                          <  1:811951/149‑1 (MQ=255)
                                                                                                    gaagaaGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGGCAGTTTGCTGTCTGCCGTTACAGTACt                                      >  4:257152/1‑149 (MQ=255)
                                                                                                      agaagaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACttt                                    <  3:65268/149‑1 (MQ=255)
                                                                                                       gaagaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTc                                   >  1:726270/1‑149 (MQ=255)
                                                                                                       gaagaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTc                                   >  3:636276/1‑149 (MQ=255)
                                                                                                       gaagaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTc                                   <  3:231369/149‑1 (MQ=255)
                                                                                                        aagaTGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCa                                  >  3:715667/1‑149 (MQ=255)
                                                                                                               gggCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGt                                                      >  3:402806/1‑122 (MQ=255)
                                                                                                               gggCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGt                                                      <  4:402806/122‑1 (MQ=255)
                                                                                                                 gCAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGACCCCCACAATCGCCCAAATGCGGCGACGAGTATCactac                                                                     >  3:279899/1‑105 (MQ=255)
                                                                                                                  cAAAGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAgccatgccat                        <  3:940389/149‑1 (MQ=255)
                                                                                                                    aaGTAGAGTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCAACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTGCAGCCATGCCATTa                      <  2:429028/149‑1 (MQ=255)
                                                                                                                         gagTGAACAGAACGAGTAGACATAGAAATCGAACTACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGtc                  >  4:994716/1‑149 (MQ=255)
                                                                                                                          agTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTTCCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGtctc                <  1:323664/149‑1 (MQ=255)
                                                                                                                          agTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGtctc                >  3:3466/1‑149 (MQ=255)
                                                                                                                           gTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTcc               >  3:448969/1‑149 (MQ=255)
                                                                                                                           gTGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTcc               >  1:630882/1‑149 (MQ=255)
                                                                                                                            tGAACAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCt              <  2:685261/149‑1 (MQ=255)
                                                                                                                               aCAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGcc           >  3:1044739/1‑149 (MQ=255)
                                                                                                                                cAGAACGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCg          >  4:775313/1‑149 (MQ=255)
                                                                                                                                   aaCGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGt                   >  4:275974/1‑137 (MQ=255)
                                                                                                                                   aaCGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTcc               >  2:752892/1‑141 (MQ=255)
                                                                                                                                   aaCGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTcc               <  1:752892/141‑1 (MQ=255)
                                                                                                                                   aaCGAGTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTaa       >  4:597011/1‑149 (MQ=255)
                                                                                                                                    aCGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTaa       <  4:941891/149‑1 (MQ=255)
                                                                                                                                        gTAGACTAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGa  <  4:715667/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGA  >  NC_000913/2725488‑2725773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: