BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,180,497	Δ1 bp	6.8%	coding (77/1161 nt)	ygiC →	ATP‑Grasp family ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,180,497	0	T	.	6.8%	405.2 / 20.6	117	coding (77/1161 nt)	ygiC	ATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand): ref base T (54/55); new base . (3/5); total (57/60)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01

Only 100 of 117 total aligned reads displayed.

AAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAA  >  NC_000913/3180356‑3180644

aaacaaaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGc                                                                                                                                               >  3:288701/1‑149 (MQ=255)

aaacaaaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGc                                                                                                                                               >  4:31323/1‑149 (MQ=255)

 aacaaaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCg                                                                                                                                              >  1:934553/1‑149 (MQ=255)

 aacaaaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCg                                                                                                                                              >  4:211065/1‑149 (MQ=255)

  acaaaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGa                                                                                                                                             >  1:477284/1‑149 (MQ=255)

     aaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGcc                                                                                                                                          <  4:418533/149‑1 (MQ=255)

      aGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTCCACACCATGTACGGCGAGCCg                                                                                                                                         <  4:510263/149‑1 (MQ=255)

           tATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑Tg                                                                                                                                   >  4:1062353/1‑149 (MQ=255)

           tATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTAcatg                                                                                                                                   <  1:1103540/149‑4 (MQ=255)

                aGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGtgtg                                                                                                                              <  1:968624/149‑1 (MQ=255)

                aGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGtgtg                                                                                                                              >  2:844019/1‑149 (MQ=255)

                  cGTAGTGCAACCGGTACCTCTTCGCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGaa                                                                                                                            <  1:71779/149‑1 (MQ=255)

                   gTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGCATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAg                                                                                                                           >  3:116964/1‑149 (MQ=255)

                     aGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGAt                                                                                                                         <  1:932571/149‑1 (MQ=255)

                       tGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGc                                                                                                                       <  3:1045108/149‑1 (MQ=255)

                       tGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGc                                                                                                                       >  1:227386/1‑149 (MQ=255)

                       tGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGc                                                                                                                       <  2:782890/149‑1 (MQ=255)

                         cAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCtt                                                                                                                     >  2:433658/1‑149 (MQ=255)

                              ggTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtactac                                                                                                                >  4:73632/1‑149 (MQ=255)

                                tACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACaa                                                                                                              <  4:633008/149‑1 (MQ=255)

                                tACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACaa                                                                                                              <  3:66635/149‑1 (MQ=255)

                                 aCCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAg                                                                                                             >  1:42623/1‑149 (MQ=255)

                                 aCCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAg                                                                                                             <  4:311729/149‑1 (MQ=255)

                                  ccTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGTCGTAC‑TGGTGTGAAGATGCTTACTACAAGt                                                                                                            <  1:865480/149‑1 (MQ=255)

                                   ctctTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTAcatggt                                                                                                                                 <  2:1040121/127‑6 (MQ=255)

                                   ctctTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTAcatggt                                                                                                                                 >  1:1040121/1‑122 (MQ=255)

                                    tcttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTg                                                                                                          >  1:791267/1‑149 (MQ=255)

                                           gTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCg                                                                                                   >  4:368253/1‑149 (MQ=255)

                                           gTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCg                                                                                                   >  4:847629/1‑149 (MQ=255)

                                               aaTGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCa                                                                                               <  2:772605/149‑1 (MQ=255)

                                               aaTGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCa                                                                                               >  2:1019814/1‑149 (MQ=255)

                                                  gggTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGt                                                                                            >  1:199680/1‑149 (MQ=255)

                                                   ggTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGtt                                                                                           <  2:1047830/149‑1 (MQ=255)

                                                       gCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGaaa                                                                                       >  1:721628/1‑149 (MQ=255)

                                                        cTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGaaaa                                                                                      <  1:917780/149‑1 (MQ=255)

                                                          gATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGc                                                                                    >  4:150175/1‑149 (MQ=255)

                                                           aTACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCt                                                                                   <  2:1088092/149‑1 (MQ=255)

                                                            tACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTg                                                                                  <  2:238354/149‑1 (MQ=255)

                                                            tACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTg                                                                                  <  3:764324/149‑1 (MQ=255)

                                                               cGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaa                                                                               <  1:717583/149‑1 (MQ=255)

                                                                 aTGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCt                                                                                   <  2:567752/143‑1 (MQ=255)

                                                                 aTGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCt                                                                                   >  1:567752/1‑143 (MQ=255)

                                                                  tGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa                                                                            <  4:233991/149‑1 (MQ=255)

                                                                  tGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa                                                                            <  1:1039197/149‑1 (MQ=255)

                                                                  tGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa                                                                            >  1:305374/1‑149 (MQ=255)

                                                                  tGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa                                                                            <  3:368253/149‑1 (MQ=255)

                                                                  tGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa                                                                            <  3:785778/149‑1 (MQ=255)

                                                                   ggAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGaagaag                                                                           >  2:126984/1‑149 (MQ=255)

                                                                    gAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGt                                                                          <  3:881417/149‑1 (MQ=255)

                                                                      aaGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCATAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCa                                                                        <  4:858435/149‑1 (MQ=255)

                                                                           tCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCTCGCCGTAcat                                                                                                                                    <  1:33391/85‑3 (MQ=255)

                                                                           tCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCTCGCCGTAcat                                                                                                                                    >  2:33391/1‑83 (MQ=255)

                                                                           tCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCAccgcc                                                                   >  3:1078182/1‑149 (MQ=255)

                                                                               tATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtac                                                                                                                   <  3:836586/97‑1 (MQ=255)

                                                                               tATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtac                                                                                                                   >  4:836586/1‑97 (MQ=255)

                                                                               tATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAAc                                                               >  2:1059542/1‑149 (MQ=255)

                                                                                    ccGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCAc                                                          >  1:782843/1‑149 (MQ=255)

                                                                                           cccGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtgt                                                   <  2:432273/149‑1 (MQ=255)

                                                                                            ccGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtgtg                                                  >  2:978653/1‑149 (MQ=255)

                                                                                            ccGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtgtg                                                  >  2:242607/1‑149 (MQ=255)

                                                                                            ccGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtgtg                                                  >  3:340878/1‑149 (MQ=255)

                                                                                              ggACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGcc                                                <  1:242607/149‑1 (MQ=255)

                                                                                                   ggCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGaaa                                           <  3:211065/149‑1 (MQ=255)

                                                                                                      gTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGa                                                      <  3:352937/135‑1 (MQ=255)

                                                                                                      gTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGa                                                      >  4:352937/1‑135 (MQ=255)

                                                                                                      gTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtg                                        <  2:782843/149‑1 (MQ=255)

                                                                                                       tGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtgg                                       <  1:433658/149‑1 (MQ=255)

                                                                                                       tGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtgg                                       >  4:52485/1‑149 (MQ=255)

                                                                                                       tGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtgg                                       <  2:1101571/149‑1 (MQ=255)

                                                                                                          gaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGt                                                                          >  4:248184/1‑111 (MQ=255)

                                                                                                          gaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGt                                                                          <  3:248184/111‑1 (MQ=255)

                                                                                                          gaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGtggtgg                                    <  3:673775/149‑1 (MQ=255)

                                                                                                               cccACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGaaaaa                               <  4:537726/149‑1 (MQ=255)

                                                                                                               cccACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGaaaaa                               <  1:1059542/149‑1 (MQ=255)

                                                                                                                ccACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGc                                                 >  4:1002261/1‑130 (MQ=255)

                                                                                                                ccACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGc                                                 <  3:1002261/130‑1 (MQ=255)

                                                                                                                ccACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAg                              >  2:313676/1‑149 (MQ=255)

                                                                                                                ccACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAg                              >  1:502528/1‑149 (MQ=255)

                                                                                                                    gAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGAt                          <  4:487334/149‑1 (MQ=255)

                                                                                                                     aaTACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATc                         <  1:128631/149‑1 (MQ=255)

                                                                                                                       tACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGtt                                                                                           >  3:566106/1‑81 (MQ=255)

                                                                                                                       tACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGtt                                                                                           <  4:566106/81‑1 (MQ=255)

                                                                                                                       tACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGc                       >  2:267460/1‑149 (MQ=255)

                                                                                                                        aCGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGcc                      >  4:205663/1‑149 (MQ=255)

                                                                                                                          ggTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAg                    >  1:1065215/1‑149 (MQ=255)

                                                                                                                           gTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGc                   >  4:74665/1‑149 (MQ=255)

                                                                                                                                aaTTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGa              >  1:397956/1‑149 (MQ=255)

                                                                                                                                 atcttCACACCA‑GTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa                                                                            >  2:74012/4‑86 (MQ=21)

                                                                                                                                 atcttCACACCA‑GTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa                                                                            <  1:74012/83‑1 (MQ=21)

                                                                                                                                  ttttCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGc                                                 >  3:140591/1‑112 (MQ=255)

                                                                                                                                  ttttCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGc                                                 <  4:140591/112‑1 (MQ=255)

                                                                                                                                  ttttCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGc            <  3:710306/149‑1 (MQ=255)

                                                                                                                                    ttCACACCA‑GTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtggt                                      >  3:937702/1‑121 (MQ=38)

                                                                                                                                     tCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtga         <  2:227386/149‑1 (MQ=255)

                                                                                                                                        cacCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGATTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgatga      <  2:477284/149‑1 (MQ=255)

                                                                                                                                         acCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAc     >  1:294640/1‑149 (MQ=255)

                                                                                                                                         acCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAc     >  3:605480/1‑149 (MQ=255)

                                                                                                                                          ccATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCCCCCAGGTTCAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGGAACAGTGATCGCCAGCGATGAGCTGATGAcc    <  4:663718/149‑1 (MQ=255)

                                                                                                                                           cATGTACGGCGAGCCGTAC‑TGGTCTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAAAAGTCACCGCCGAACTGCACCAGATGGGCCTGAAAGTGGTGGAAAAATTGATCGCCAGCGATGAGCTGATGACCa   >  2:616838/1‑149 (MQ=255)

                                                                                                                                            aTGTACGGCGCGGCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCaa  >  2:607416/1‑149 (MQ=255)

AAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAA  >  NC_000913/3180356‑3180644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`