Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,888,212 Δ1 bp 5.3% intergenic (+20/‑223) mnmE → / → tnaC tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,888,2120T. 5.3% 326.1 / 10.7 95intergenic (+20/‑223)mnmE/tnaCtRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide
Reads supporting (aligned to +/- strand):  ref base T (49/41);  new base . (2/3);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

CTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACA  >  NC_000913/3888066‑3888359
                                                                                                                                                  |                                                                                                                                                    
cTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTc                                                                                                                                                    <  4:132349/149‑1 (MQ=255)
cTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTCGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTc                                                                                                                                                    >  4:440960/1‑149 (MQ=255)
   ggCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGa                                                                                                                                                 <  3:83367/149‑1 (MQ=255)
    gCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGAc                                                                                                                                                <  4:65331/149‑1 (MQ=255)
       ggTGAACTGCAGGCGGTAGAGTTGCGTCTGGCCCAGCAGAACTTAAGTGAAATGACCGGGGAATTTACTTCAGACGACCTGCTGGGCCGGACTTTCTCCAGCTTCTGTATTAGTAAGTAACCGCGCTTACGAACCCGCATTCTGACTGt                                                                                                                                             >  2:1018673/1‑149 (MQ=255)
        gTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTc                                                                                                                                            >  1:368117/1‑149 (MQ=255)
        gTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTGTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCCTTCTGACTGTc                                                                                                                                            <  2:701448/149‑1 (MQ=255)
            aCTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGAt                                                                                                                                       <  1:574486/149‑1 (MQ=255)
              tgctgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGc                                                                                                                                     <  1:1036337/149‑1 (MQ=255)
              tgctgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGc                                                                                                                                     <  4:829251/149‑1 (MQ=255)
                 tgGCGGAAGAGTTGCGTCTGGCGCAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                                                                  <  3:611937/149‑1 (MQ=255)
                 tgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgc                                                                                                                                  <  3:53340/149‑1 (MQ=255)
                 tgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                                                                  <  3:267535/149‑1 (MQ=255)
                  ggCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCACATTCTGACTGTCAGATGCGgct                                                                                                                                 >  2:1017590/1‑149 (MQ=255)
                    cGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttc                                                                                                                               <  1:28532/149‑1 (MQ=255)
                           gTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAt                                                                                                                        <  3:273574/149‑1 (MQ=255)
                           gTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAt                                                                                                                        >  2:900282/1‑149 (MQ=255)
                            ttGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAtt                                                                                                                       <  4:834063/149‑1 (MQ=255)
                            ttGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAtt                                                                                                                       <  2:139865/149‑1 (MQ=255)
                            ttGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAtt                                                                                                                       >  4:373935/1‑149 (MQ=255)
                               cGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGCGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACAGTCAGATGCGGCTTCGCTTCATTGtt                                                                                                                    <  2:308087/149‑1 (MQ=255)
                                gTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGTCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTa                                                                                                                   >  3:171689/1‑149 (MQ=255)
                                 tCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGTCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                                                                  <  4:171689/149‑1 (MQ=255)
                                 tCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                                                                  <  4:109358/149‑1 (MQ=255)
                                 tCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                                                                  <  1:1049870/149‑1 (MQ=255)
                                       acaGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTcc                                                                                                            >  4:72742/1‑149 (MQ=255)
                                          gcagAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGt                                                                                                         >  4:335833/1‑149 (MQ=255)
                                          gcagAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGt                                                                                                         >  1:1003406/1‑149 (MQ=255)
                                            agAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTa                                                                                                       >  4:339092/1‑149 (MQ=255)
                                             gAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAt                                                                                                      >  1:52902/1‑149 (MQ=255)
                                                cTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTg                                                                                                          >  4:755597/1‑142 (MQ=255)
                                                cTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTg                                                                                                          <  3:755597/142‑1 (MQ=255)
                                                cTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTcc                                                                                                   <  1:716117/149‑1 (MQ=255)
                                                 ttAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCt                                                                                                  >  1:382677/1‑149 (MQ=255)
                                                 ttAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCt                                                                                                  >  1:132436/1‑149 (MQ=255)
                                                             aCCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgc                                                                                                                             <  1:986959/110‑1 (MQ=255)
                                                             aCCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgc                                                                                                                             >  2:986959/1‑110 (MQ=255)
                                                             aCCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttttt                                                                                      >  2:453395/1‑149 (MQ=255)
                                                                 gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa                                                                                  <  2:531916/149‑1 (MQ=255)
                                                                 gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa                                                                                  <  3:339092/149‑1 (MQ=255)
                                                                 gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa                                                                                  <  3:467374/149‑1 (MQ=255)
                                                                 gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa                                                                                  >  2:38376/1‑149 (MQ=255)
                                                                 gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa                                                                                  <  4:491631/149‑1 (MQ=255)
                                                                      tttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTa                                                                             >  2:903343/1‑149 (MQ=255)
                                                                      tttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTa                                                                             >  3:780297/1‑149 (MQ=255)
                                                                           ttCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAAtt                                                                        >  4:963469/1‑149 (MQ=255)
                                                                           ttCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAAtt                                                                        >  1:424919/1‑149 (MQ=255)
                                                                            tCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTc                                                                       <  3:64133/149‑1 (MQ=255)
                                                                              aGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCtt                                                                     >  2:416484/1‑149 (MQ=255)
                                                                               gacgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTa                                                                    >  1:270988/1‑149 (MQ=255)
                                                                                acgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTAc                                                                   <  2:769574/149‑1 (MQ=255)
                                                                                acgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTAc                                                                   <  2:270988/149‑1 (MQ=255)
                                                                                  gacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGt                                                                 >  3:39321/1‑149 (MQ=255)
                                                                                   acCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTa                                                                >  3:308106/1‑149 (MQ=255)
                                                                                         tgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTtat                                                          <  1:416484/149‑1 (MQ=255)
                                                                                               ggATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCtt                                                    <  2:52902/149‑1 (MQ=255)
                                                                                                gATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCttt                                                   >  2:298690/1‑149 (MQ=255)
                                                                                                       tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaaaaaaa                                            <  3:72742/149‑1 (MQ=255)
                                                                                                       tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaaaaaaa                                            <  1:659333/149‑1 (MQ=255)
                                                                                                       tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaaaaaaa                                            <  3:883459/149‑1 (MQ=255)
                                                                                                        ccAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAg                                           <  1:854371/149‑1 (MQ=255)
                                                                                                         cAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgctt                                                                                                                           >  4:548291/1‑68 (MQ=255)
                                                                                                         cAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgctt                                                                                                                           <  3:548291/68‑1 (MQ=255)
                                                                                                         cAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGc                                          >  3:269183/1‑149 (MQ=255)
                                                                                                         cAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGc                                          <  3:335833/149‑1 (MQ=255)
                                                                                                          aGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttttttt                                                                                     >  3:197565/1‑105 (MQ=255)
                                                                                                          aGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttttttt                                                                                     <  4:197565/105‑1 (MQ=255)
                                                                                                          aGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCa                                         >  3:152266/1‑149 (MQ=255)
                                                                                                          aGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCa                                         >  1:769759/1‑149 (MQ=255)
                                                                                                           gCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTa                                                  >  2:968956/1‑139 (MQ=255)
                                                                                                           gCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTa                                                  <  1:968956/139‑1 (MQ=255)
                                                                                                             ttCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCAttt                                      >  2:671061/1‑149 (MQ=255)
                                                                                                               cTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTaa                                    <  1:893545/149‑1 (MQ=255)
                                                                                                                tGTATTGGTAAGTAACCGCGCTTACGAAGCCACATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAat                                   <  1:1017590/149‑1 (MQ=255)
                                                                                                                 gTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAata                                  >  1:571532/1‑149 (MQ=255)
                                                                                                                  tATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACATAATTTATAATCTTTAAAAAAAGCATTTAatat                                 >  3:118879/1‑149 (MQ=255)
                                                                                                                       gtaagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTc                            >  4:224050/1‑149 (MQ=255)
                                                                                                                       gtaagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTc                            >  3:546190/1‑149 (MQ=255)
                                                                                                                        taagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTcc                           >  1:504826/1‑149 (MQ=255)
                                                                                                                          agtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTcccc                         >  4:948972/1‑149 (MQ=255)
                                                                                                                               ccGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACg                    >  2:105401/1‑149 (MQ=255)
                                                                                                                                         gAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattc          >  3:99607/1‑149 (MQ=255)
                                                                                                                                         gAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattc          >  4:985917/1‑149 (MQ=255)
                                                                                                                                         gAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattc          >  4:373925/1‑149 (MQ=255)
                                                                                                                                           aGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattcga        >  4:329403/1‑149 (MQ=255)
                                                                                                                                           aGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattcga        >  4:74984/1‑149 (MQ=255)
                                                                                                                                            gCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattcgat       <  1:613865/149‑1 (MQ=255)
                                                                                                                                            gCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattcgat       <  3:963469/149‑1 (MQ=255)
                                                                                                                                             ccGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgattcgatt      <  3:469420/149‑1 (MQ=255)
                                                                                                                                             ccGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaa                                                 >  2:1051972/1‑106 (MQ=38)
                                                                                                                                             ccGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaa                                                 <  1:1051972/106‑1 (MQ=38)
                                                                                                                                               gCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTca    >  4:1069295/1‑149 (MQ=255)
                                                                                                                                               gCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACGCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAACATTTATTATTGCTCCCCGAACTATTGTGATTCGATTca    >  4:302690/1‑149 (MQ=255)
                                                                                                                                                 aTTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTcaca  >  2:717802/1‑149 (MQ=255)
                                                                                                                                                 aTTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTcaca  >  1:1019272/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACA  >  NC_000913/3888066‑3888359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: