Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,017,216:1 +A 5.7% intergenic (+24/‑117) udp → / → rmuC uridine phosphorylase/DNA recombination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,017,2161.A 5.7% 660.7 / 33.7 157intergenic (+24/‑117)udp/rmuCuridine phosphorylase/DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base . (80/68);  new base A (3/6);  total (83/74)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

CGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTAT  >  NC_000913/4017069‑4017364
                                                                                                                                                    |                                                                                                                                                    
cGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGa                                                                                                                                                     >  2:93347/1‑149 (MQ=255)
 ggTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGAc                                                                                                                                                    <  1:623517/149‑1 (MQ=255)
     tGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGt                                                                                                                                                <  2:651369/149‑1 (MQ=255)
      ggTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTACTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGtt                                                                                                                                               >  2:693083/1‑149 (MQ=255)
       gTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTc                                                                                                                                              >  1:120083/1‑149 (MQ=255)
          gCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGc                                                                                                                                           >  1:348692/1‑149 (MQ=255)
           cGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGAACGCGTCGGcc                                                                                                                                          <  1:1050647/149‑1 (MQ=255)
            gggTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCt                                                                                                                                         >  2:899839/1‑149 (MQ=255)
              gtgtTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGAACGCGTCGGCCttc                                                                                                                                       <  3:173492/149‑2 (MQ=255)
               tgtTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGAACGCGTCGGCCttca                                                                                                                                      <  1:451546/149‑3 (MQ=255)
               tgtTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCtttt                                                                                                                                      <  3:129203/149‑1 (MQ=255)
               tgtTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCtttt                                                                                                                                      <  1:549157/149‑1 (MQ=255)
                 ttATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGt                                                                                                                                    <  1:416564/149‑1 (MQ=255)
                   aTCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAt                                                                                                                                  >  2:1074798/1‑149 (MQ=255)
                    tCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGCGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAtt                                                                                                                                 <  4:731407/149‑1 (MQ=255)
                    tCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAtt                                                                                                                                 >  1:775055/1‑149 (MQ=255)
                      gTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAtttt                                                                                                                               >  2:467349/1‑149 (MQ=255)
                      gTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAtttt                                                                                                                               >  3:613680/1‑149 (MQ=255)
                      gTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAtttt                                                                                                                               <  3:680049/149‑1 (MQ=255)
                       ttAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTAttttt                                                                                                                              <  1:467349/149‑1 (MQ=255)
                           ccGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGt                                                                                                                          >  3:23868/1‑149 (MQ=255)
                               aCCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAgcg                                                                                                                      >  1:692173/1‑149 (MQ=255)
                                 ccAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCTTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGcc                                                                                                                    >  4:858693/1‑149 (MQ=255)
                                 ccAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGcc                                                                                                                    >  1:147353/1‑149 (MQ=255)
                                  cAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCt                                                                                                                   >  1:259752/1‑149 (MQ=255)
                                    gCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCg                                                                                                                 >  4:356361/1‑149 (MQ=255)
                                           aTCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGaaa                                                                                                          >  1:717666/1‑149 (MQ=255)
                                              ccGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGc                                                                                                       <  2:1066929/149‑1 (MQ=255)
                                              ccGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGc                                                                                                       <  2:16241/149‑1 (MQ=255)
                                               cGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGcc                                                                                                      <  4:87127/149‑1 (MQ=255)
                                                    gCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTcc                                                                                                 <  2:692173/149‑1 (MQ=255)
                                                      tGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCaa                                                                                               >  3:342051/1‑149 (MQ=255)
                                                      tGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCaa                                                                                               <  2:503313/149‑1 (MQ=255)
                                                       gagaCGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTTGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAAc                                                                                              <  4:711448/149‑1 (MQ=255)
                                                        agaCGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGAACGCGTCGGCCttca                                                                                                                                      >  2:423670/1‑106 (MQ=255)
                                                         gaCGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGTCTTTCCAACTg                                                                                            <  3:541384/149‑1 (MQ=255)
                                                            gATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGAc                                                                                         >  3:133294/1‑149 (MQ=255)
                                                              tGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGt                                                                                       <  2:530458/149‑1 (MQ=255)
                                                                aaacaaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGttt                                                                                     <  3:676669/149‑1 (MQ=255)
                                                                aaacaaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGttt                                                                                     <  4:557563/149‑1 (MQ=255)
                                                                 aacaaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCATCGTCTGCTGTAATTCTCTTCTCCTGTCTGGAGGCCGACGCGTTCGGGCTTTTGTATTTTTTCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGTCGTTTg                                                                                    >  4:45162/1‑149 (MQ=255)
                                                                  acaaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGt                                                                                   >  3:316563/1‑149 (MQ=255)
                                                                    aaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTAc                                                                                 >  1:395411/1‑149 (MQ=255)
                                                                    aaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTAc                                                                                 <  4:1012626/149‑1 (MQ=255)
                                                                      acCGAAAGGCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGcc                                                                                                                    <  1:854344/112‑1 (MQ=255)
                                                                      acCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAg                                                                               >  4:35245/1‑149 (MQ=255)
                                                                        cGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGca                                                                             >  4:971402/1‑149 (MQ=255)
                                                                              ccATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTc                                                                       >  4:387781/1‑149 (MQ=255)
                                                                              ccATGCGGCGAAATTCGTGGTGGAAGCGGCGCGTCGTCTGCGGTAATTCTCTTCTCCTGTCTGAGGGGCGACGCGTTCTGCCTTTTGTATTTTTGCGTAGCGCCTCGGAGGAAATGCCTTTCCAACTGGGCGTTTGTGCAGCACAATTc                                                                       >  2:413187/1‑149 (MQ=255)
                                                                                aTGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTa                                                                     <  4:613680/149‑1 (MQ=255)
                                                                                  gCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTAtt                                                                   <  2:648097/149‑1 (MQ=255)
                                                                                     gTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTtg                                                                >  2:632734/1‑149 (MQ=255)
                                                                                      tGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTtgt                                                               >  1:776961/1‑149 (MQ=255)
                                                                                        aaaaTCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGc                                                             <  3:764210/149‑1 (MQ=255)
                                                                                          aaTCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCgg                                                           <  2:775055/149‑1 (MQ=255)
                                                                                              gtggtgGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTaa                                                       <  3:283593/149‑1 (MQ=255)
                                                                                              gtggtgGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTaa                                                       <  2:820637/149‑1 (MQ=255)
                                                                                                 gtggAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAgtt                                                    <  4:780523/149‑1 (MQ=255)
                                                                                                 gtggAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAgtt                                                    >  3:405139/1‑149 (MQ=255)
                                                                                                    gAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAgttgtt                                                 >  2:179053/1‑149 (MQ=255)
                                                                                                      aGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGc                                               <  4:1015613/149‑1 (MQ=255)
                                                                                                       gcggcgCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCg                                              >  4:415386/1‑149 (MQ=255)
                                                                                                        cggcgCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGt                                             <  4:806639/149‑1 (MQ=255)
                                                                                                            gcgTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACTCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTCTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTCGTTGCGTCagg                                         <  1:899839/149‑1 (MQ=255)
                                                                                                             cgtcgtCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCagga                                        <  3:77076/149‑1 (MQ=255)
                                                                                                                     gctgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTtgt                                <  1:645444/149‑1 (MQ=255)
                                                                                                                     gctgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTtgt                                >  2:270706/1‑149 (MQ=255)
                                                                                                                      ctgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTtgtg                               <  3:618348/149‑1 (MQ=255)
                                                                                                                       tgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGttt                                                                                     <  3:824230/94‑1 (MQ=255)
                                                                                                                       tgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTgg                              <  3:415386/149‑1 (MQ=255)
                                                                                                                       tgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTgg                              >  1:181501/1‑149 (MQ=255)
                                                                                                                       tgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTgg                              >  4:958418/1‑149 (MQ=255)
                                                                                                                        gTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCGGGAGGCGTTGTGGa                             <  4:555681/149‑1 (MQ=255)
                                                                                                                        gTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGt                                   <  2:126362/143‑1 (MQ=255)
                                                                                                                          aaTTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGAtt                           >  1:1027047/1‑149 (MQ=255)
                                                                                                                           aTTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAg                                                                               <  3:73234/96‑1 (MQ=255)
                                                                                                                           aTTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGAttt                          >  3:877002/1‑149 (MQ=255)
                                                                                                                           aTTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGAttt                          <  4:23868/149‑1 (MQ=255)
                                                                                                                           aTTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGAttt                          >  4:408959/1‑149 (MQ=255)
                                                                                                                            ttctcttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTtc                         >  1:40692/1‑149 (MQ=255)
                                                                                                                            ttctcttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTtc                         >  1:292442/1‑149 (MQ=255)
                                                                                                                               tcttctcCTGTCTGAAGGCCGCCGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCATCTGGACGTTTGTACAGCACACTTCTATTTTGTGCGGGTAAGTCGTTGCTTCAGGAGGCGTTGTGGATTTCTCa                      <  2:84185/149‑1 (MQ=255)
                                                                                                                               tcttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCa                      >  4:393675/1‑149 (MQ=255)
                                                                                                                                cttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCaa                     >  4:473943/1‑149 (MQ=255)
                                                                                                                                 ttctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAAt                    >  2:849834/1‑149 (MQ=255)
                                                                                                                                    tcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCAt                 >  1:363520/1‑149 (MQ=255)
                                                                                                                                     ccTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATg                <  2:1022657/149‑1 (MQ=255)
                                                                                                                                     ccTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATg                <  4:133294/149‑1 (MQ=255)
                                                                                                                                     ccTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATg                <  3:329277/149‑1 (MQ=255)
                                                                                                                                      cTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATgg               >  2:492971/1‑149 (MQ=255)
                                                                                                                                      cTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATgg               >  4:915378/1‑149 (MQ=255)
                                                                                                                                      cTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATgg               >  4:468475/1‑149 (MQ=255)
                                                                                                                                          cTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGAACACTTCTATTTGGTGCGGGGAAGAGCTTGCGTCAGGAGGCGTTGTGGATGTCTCAAACATGGTTTa           >  4:157189/1‑149 (MQ=255)
                                                                                                                                           tGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTAc          <  2:348692/149‑1 (MQ=255)
                                                                                                                                           tGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTAc          <  3:7824/149‑1 (MQ=255)
                                                                                                                                           tGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTAc          >  1:1087967/1‑149 (MQ=255)
                                                                                                                                           aaaaGGCCGAACGCGTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGc                                                             <  2:300116/96‑1 (MQ=255)
                                                                                                                                           aaaaGGCCGAACGCGTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGc                                                             >  1:300116/3‑98 (MQ=255)
                                                                                                                                            gAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACTATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACg         >  2:81107/1‑149 (MQ=255)
                                                                                                                                                   gACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCACTCATGGTTTACGCAGTTAt  >  4:737088/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTAT  >  NC_000913/4017069‑4017364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: