Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,141,150 G→A 9.3% intergenic (+164/+32) flgL → / ← rne flagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,141,1500GA 9.3% 317.7 / 16.9 107intergenic (+164/+32)flgL/rneflagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base G (41/56);  new base A (6/4);  total (47/60)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 107 total aligned reads displayed.

ATCATGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGA  >  NC_000913/1141003‑1141296
                                                                                                                                                   |                                                                                                                                                  
atCATGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGt                                                                                                                                                   >  4:3676/1‑149 (MQ=255)
 tCATGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGACTTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGtt                                                                                                                                                  >  3:369432/1‑149 (MQ=255)
    tGAAAGTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTAca                                                                                                                                               >  2:132065/1‑148 (MQ=255)
    tGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTAcc                                                                                                                                               >  3:564550/1‑149 (MQ=255)
     gAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCa                                                                                                                                              >  2:7937/1‑149 (MQ=255)
     gAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCa                                                                                                                                              <  2:814419/149‑1 (MQ=255)
     gAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCa                                                                                                                                              <  2:1045485/149‑1 (MQ=255)
     gAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTAGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGACTCAAAGATGAAATAAAAGAGCCCTGGCAGTTACCa                                                                                                                                              <  2:87330/149‑1 (MQ=255)
       aaCTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAgg                                                                                                                                            >  2:1029532/1‑149 (MQ=255)
         cTGGGTATGTTTTGTCTGCCGGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGc                                                                                                                                          <  2:994058/149‑1 (MQ=255)
          tGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCt                                                                                                                                         <  3:319723/149‑1 (MQ=255)
            ggTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTg                                                                                                                                       <  1:711466/149‑1 (MQ=255)
             gTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGGATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGTAACTGCCAGGGCtttt                                                                                                                                      <  2:958906/149‑3 (MQ=255)
             gTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCtt                                                                                                                                        <  4:799809/147‑1 (MQ=255)
             gTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCtt                                                                                                                                        >  3:799809/1‑147 (MQ=255)
             gTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGa                                                                                                                                      <  2:775502/149‑1 (MQ=255)
              tATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTTATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGAt                                                                                                                                     <  2:1052930/149‑1 (MQ=255)
              tATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGAt                                                                                                                                     <  4:564201/149‑1 (MQ=255)
              tATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGAt                                                                                                                                     >  1:425500/1‑149 (MQ=255)
               aTGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGTAACTGCCAGGGCtttttt                                                                                                                                    <  1:721974/149‑5 (MQ=255)
               aTGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGAtt                                                                                                                                    <  4:369432/149‑1 (MQ=255)
                  ttttGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACt                                                                                                                                 >  2:147120/1‑149 (MQ=255)
                  ttttGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACt                                                                                                                                 >  3:111810/1‑149 (MQ=255)
                     tGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTg                                                                                                                              <  3:859265/149‑1 (MQ=255)
                      gTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGa                                                                                                                             <  3:423388/149‑1 (MQ=255)
                        ctgcctgcTCTGGGATCGCTGGGGCGGGCATTTTTTTACCTATTTTCCATTGTTGGTTAGTAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTATGAGc                                                                                                                           <  3:452547/149‑1 (MQ=255)
                         tgcctgcTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCt                                                                                                                          >  3:176688/1‑149 (MQ=255)
                           cctgcTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTaa                                                                                                                        >  1:756856/1‑149 (MQ=255)
                            ctgcTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAat                                                                                                                       >  1:951971/1‑149 (MQ=255)
                               ctctGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAattat                                                                                                                    <  4:242388/149‑1 (MQ=255)
                                tctGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAattatt                                                                                                                   >  4:213847/1‑149 (MQ=255)
                                tctGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAattatt                                                                                                                   <  1:1067225/149‑1 (MQ=255)
                                 ctGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAAttatta                                                                                                                  <  1:483770/149‑1 (MQ=255)
                                 ctGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAAttatta                                                                                                                  <  4:529148/149‑1 (MQ=255)
                                  tGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTAc                                                                                                                 <  3:806758/149‑1 (MQ=255)
                                   gggATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCtt                                                                                                                                        >  1:891175/1‑125 (MQ=255)
                                   gggATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCtt                                                                                                                                        <  2:891175/125‑1 (MQ=255)
                                     gATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCa                                                                                                              >  4:850475/1‑149 (MQ=255)
                                        cGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACa                                                                                                           <  3:449518/149‑1 (MQ=255)
                                           tGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGt                                                                                                        <  4:265661/149‑1 (MQ=255)
                                            ggggCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGtt                                                                                                       <  1:108328/149‑1 (MQ=255)
                                                    cATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGAcgc                                                                                               <  4:554123/149‑1 (MQ=255)
                                                     aTTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGAcgcg                                                                                              >  2:937318/1‑149 (MQ=255)
                                                                tATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCa                                                                                   <  3:128058/149‑1 (MQ=255)
                                                                  ttttGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGAcgc                                                                                               <  3:1026514/135‑1 (MQ=255)
                                                                  ttttGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGAcgc                                                                                               >  4:1026514/1‑135 (MQ=255)
                                                                   tttGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCagag                                                                                <  2:71801/149‑1 (MQ=255)
                                                                       cATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCat                                                                            <  4:564550/149‑1 (MQ=255)
                                                                         ttgttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGct                                                                                      >  2:981329/1‑136 (MQ=255)
                                                                         ttgttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCg                                                                                      <  1:981329/137‑1 (MQ=255)
                                                                         ttgttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCatga                                                                          <  3:619137/149‑1 (MQ=255)
                                                                          tgttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCatgat                                                                         <  1:463916/149‑1 (MQ=255)
                                                                           gttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCatgatg                                                                        <  4:790688/149‑1 (MQ=255)
                                                                              ggTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGAc                                                                                                 <  2:1056111/121‑1 (MQ=255)
                                                                              ggTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGAc                                                                                                 >  1:1056111/1‑121 (MQ=255)
                                                                              ggTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATgtgt                                                                     <  1:309878/149‑1 (MQ=255)
                                                                               gTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGtt                                                                    >  1:655823/1‑149 (MQ=255)
                                                                                  aGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGcc                                                                 <  4:940177/149‑1 (MQ=255)
                                                                                  aGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGcc                                                                 >  3:335564/1‑149 (MQ=255)
                                                                                   gCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCg                                                                >  3:638107/1‑149 (MQ=255)
                                                                                    cAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGt                                                               >  3:109254/1‑149 (MQ=255)
                                                                                       ggATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATg                                                            <  1:87524/149‑1 (MQ=255)
                                                                                         aTGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGac                                                          <  1:114509/149‑1 (MQ=255)
                                                                                              aTTCGATGGATTTTAATATGTTGACTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCt                                                     <  2:648332/149‑1 (MQ=255)
                                                                                               ttCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTg                                                    >  1:1070821/1‑149 (MQ=255)
                                                                                                 cGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCg                                                  <  4:306070/149‑1 (MQ=255)
                                                                                                   aTGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGc                                                >  4:213201/1‑149 (MQ=255)
                                                                                                   aTGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGc                                                >  4:638962/1‑149 (MQ=255)
                                                                                                       aTTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCt                                            >  3:729322/1‑149 (MQ=255)
                                                                                                           tAATATGTTGCTTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATCTGTTGCCGTATGACCACCTGCGGCACCTTTAc                                        <  4:924751/149‑1 (MQ=255)
                                                                                                           tAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTCTTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTAc                                        <  2:835999/149‑1 (MQ=255)
                                                                                                               atGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTc                                    <  4:111810/149‑1 (MQ=255)
                                                                                                                 gTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGa                                  <  4:448342/149‑1 (MQ=255)
                                                                                                                  ttGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGATGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGaa                                 <  2:8112/149‑1 (MQ=255)
                                                                                                                  ttGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGaa                                 <  2:682786/149‑1 (MQ=255)
                                                                                                                     aTTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGc                              <  3:996272/149‑1 (MQ=255)
                                                                                                                      ttCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCa                             >  1:16129/1‑149 (MQ=255)
                                                                                                                         aaaGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCaaaa                          <  4:130737/149‑1 (MQ=255)
                                                                                                                         aaaGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCaaaa                          <  2:425500/149‑1 (MQ=255)
                                                                                                                          aaGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAg                         >  2:343852/1‑149 (MQ=255)
                                                                                                                             aTGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAg                      <  3:213201/149‑1 (MQ=255)
                                                                                                                              tGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAgg                     <  1:132065/149‑1 (MQ=255)
                                                                                                                              tGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAgg                     <  4:860210/149‑1 (MQ=255)
                                                                                                                                     aaaaaGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGc              <  4:109254/149‑1 (MQ=255)
                                                                                                                                     aaaaaGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGc              >  3:372153/1‑149 (MQ=255)
                                                                                                                                     aaaaaGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCCTCGAAGGCAAAAGTAGGGCGCTGc              <  1:937318/149‑1 (MQ=255)
                                                                                                                                      aaaaGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGcc             >  1:961070/1‑149 (MQ=255)
                                                                                                                                      aaaaGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGcc             >  1:386680/1‑149 (MQ=255)
                                                                                                                                       caaGCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCa            >  4:25342/2‑149 (MQ=255)
                                                                                                                                       caaGCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCa            >  2:714944/2‑149 (MQ=255)
                                                                                                                                       aaaGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCa            >  4:1009671/1‑149 (MQ=255)
                                                                                                                                        aaGCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCt                <  4:1078617/144‑1 (MQ=255)
                                                                                                                                        aaGCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCt                >  3:1078617/1‑144 (MQ=255)
                                                                                                                                        aaGCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAg           >  2:1081870/1‑149 (MQ=255)
                                                                                                                                          gCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAgg                               <  2:313913/127‑1 (MQ=255)
                                                                                                                                          gCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTc         >  3:922788/1‑149 (MQ=255)
                                                                                                                                             cTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACt      >  3:532643/1‑149 (MQ=255)
                                                                                                                                             cTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACt      >  3:262391/1‑149 (MQ=255)
                                                                                                                                             cTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACt      >  3:295790/1‑149 (MQ=255)
                                                                                                                                                 cAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGa  <  3:523737/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCATGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGA  >  NC_000913/1141003‑1141296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: