Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,147,548 T→G 8.1% intergenic (+8/‑73) rpmF → / → plsX 50S ribosomal subunit protein L32/putative phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,147,5480TG 8.1% 450.7 / 28.3 136intergenic (+8/‑73)rpmF/plsX50S ribosomal subunit protein L32/putative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (63/62);  new base G (6/5);  total (69/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01

Only 100 of 137 total aligned reads displayed.

AAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTG  >  NC_000913/1147400‑1147695
                                                                                                                                                    |                                                                                                                                                   
aaaCGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAt                                                                                                                                                     >  4:611263/1‑149 (MQ=255)
     tGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCg                                                                                                                                                >  3:868612/1‑149 (MQ=255)
      ggCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGt                                                                                                                                               <  3:953250/149‑1 (MQ=255)
       gCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtg                                                                                                                                              >  3:1065847/1‑149 (MQ=255)
        cATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtga                                                                                                                                             <  2:734986/149‑1 (MQ=255)
        cATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtga                                                                                                                                             <  3:417510/149‑1 (MQ=255)
         aTGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCTGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtgat                                                                                                                                            >  4:235179/1‑149 (MQ=255)
         aTGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtgat                                                                                                                                            <  1:243348/149‑1 (MQ=255)
         aTGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAGATGCGtgat                                                                                                                                            <  1:923042/149‑1 (MQ=255)
          tGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAGATGCGtgatt                                                                                                                                           <  3:206645/149‑2 (MQ=255)
            cgtcgtTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGaa                                                                                                                                         <  1:991662/149‑1 (MQ=255)
              tcgtTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGc                                                                                                                                       >  1:584449/1‑149 (MQ=255)
                 ttCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTa                                                                                                                                    <  3:909156/149‑1 (MQ=255)
                 ttCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAAGCACGCATCTGCGTGATGAATCTTa                                                                                                                                    <  1:938708/149‑1 (MQ=255)
                   cccATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGt                                                                                                                                  <  1:705727/149‑1 (MQ=255)
                   cccATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGt                                                                                                                                  <  4:322070/149‑1 (MQ=255)
                    ccATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTg                                                                                                                                 <  2:624767/149‑1 (MQ=255)
                     cATCACGCGCTGATCGCGGTCACCAGCCTGTTTCTGGACAAAACTTCTGGGGCAAAACACTTGCGTCACCACATCACTGCTGACTGTTACTACCGCGGCCGCCAGGTCATCGCTAAGTAATCACTCATCTGCGCGATCAAGCTTGGTGa                                                                                                                                >  2:957491/1‑149 (MQ=255)
                       taaCGAGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTACTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAgg                                                                                                                              <  2:1031273/147‑1 (MQ=255)
                       tGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAgg                                                                                                                              <  4:751819/149‑1 (MQ=255)
                         aCGCGCTGACCGCAGTGACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGACGCTTAGTGAGGAt                                                                                                                            >  4:913887/1‑149 (MQ=255)
                              cTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTcc                                                                                                                       <  1:870346/149‑1 (MQ=255)
                               tGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTccc                                                                                                                      >  1:759606/1‑149 (MQ=255)
                                 aCCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCa                                                                                                                    >  1:447399/1‑149 (MQ=255)
                                 aCCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCa                                                                                                                    >  3:604572/1‑149 (MQ=255)
                                     cAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCa                                                                                                                >  3:112415/1‑149 (MQ=255)
                                         cACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTg                                                                                                            >  4:1069064/1‑149 (MQ=255)
                                            cAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTgggg                                                                                                         <  1:187764/149‑1 (MQ=255)
                                            cAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTgggg                                                                                                         >  1:11780/1‑149 (MQ=255)
                                              gCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGaa                                                                                                       >  3:488283/1‑149 (MQ=255)
                                              gCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGaa                                                                                                       <  3:39357/149‑1 (MQ=255)
                                              gCCTGTCTGTAGACAAAACTTCTGGCGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGaa                                                                                                       >  4:830802/1‑149 (MQ=255)
                                               ccTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGaaa                                                                                                      <  3:736696/149‑1 (MQ=255)
                                                ctgtctgtAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtga                                                                                                                                             <  4:454198/109‑1 (MQ=255)
                                                 tgtctgtAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGa                                                                                                    <  4:259454/149‑1 (MQ=255)
                                                 tgtctgtAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGa                                                                                                    <  4:417937/149‑1 (MQ=255)
                                                  gtctgtAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGAc                                                                                                   <  2:838583/149‑1 (MQ=255)
                                                      gtAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCaaa                                                                                               >  4:814383/1‑149 (MQ=255)
                                                          aCAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCgg                                                                                           >  2:589169/1‑149 (MQ=255)
                                                          aCAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCgg                                                                                           >  3:126793/1‑149 (MQ=255)
                                                            aaaaCTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGggc                                                                                         >  1:1095532/1‑149 (MQ=255)
                                                            aaaaCTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGggc                                                                                         >  2:439584/1‑149 (MQ=255)
                                                             aaaCTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGa                                                                                                                                <  3:605899/109‑1 (MQ=255)
                                                              aaCTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGggcgg                                                                                       >  3:1016451/1‑149 (MQ=255)
                                                                  tCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGcgac                                                                                   <  4:505166/149‑1 (MQ=255)
                                                                      gTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTccc                                                                                                                      <  4:195024/110‑1 (MQ=255)
                                                                      gTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTccc                                                                                                                      >  3:195024/1‑110 (MQ=255)
                                                                      gTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATa                                                                               >  2:44211/1‑149 (MQ=255)
                                                                       tGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATAc                                                                              >  1:435686/1‑149 (MQ=255)
                                                                        gAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATAcc                                                                             >  1:198963/1‑149 (MQ=255)
                                                                        gAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATAcc                                                                             >  3:235823/1‑149 (MQ=255)
                                                                         aaaaaCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGcga                                                                                    <  4:216765/141‑1 (MQ=255)
                                                                         aaaaaCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGcga                                                                                    >  3:216765/1‑141 (MQ=255)
                                                                         aaaaaCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAGATGCGtgattac                                                                                                                                         <  2:407154/88‑4 (MQ=255)
                                                                         aaaaaCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAGATGCGtgattac                                                                                                                                         >  1:407154/1‑85 (MQ=255)
                                                                               acCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGacac                                                                      <  4:534949/149‑1 (MQ=255)
                                                                               acCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGacac                                                                      >  1:85563/1‑149 (MQ=255)
                                                                                ccTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACg                                                                     >  4:115666/1‑149 (MQ=255)
                                                                                 cTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGggcggc                                                                                      >  4:460951/1‑131 (MQ=255)
                                                                                 cTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGggcggc                                                                                      <  3:460951/131‑1 (MQ=255)
                                                                                 cTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGt                                                                    >  3:288279/1‑149 (MQ=255)
                                                                                 cTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGt                                                                    >  3:888140/1‑149 (MQ=255)
                                                                                   gCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCt                                                                  <  2:211340/149‑1 (MQ=255)
                                                                                      tCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAAc                                                               >  2:619928/1‑149 (MQ=255)
                                                                                         ccacATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCt                                                            <  4:488283/149‑1 (MQ=255)
                                                                                          cacaTCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTg                                                           >  1:613201/1‑149 (MQ=255)
                                                                                          cacaTCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTg                                                           >  1:908427/1‑149 (MQ=255)
                                                                                          cacaTCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTg                                                           <  3:952134/149‑1 (MQ=255)
                                                                                             aTCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCAGGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCg                                                        >  2:944311/1‑149 (MQ=255)
                                                                                             aTCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCg                                                        >  4:636129/1‑149 (MQ=255)
                                                                                               cACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGcgac                                                                                   >  3:964149/1‑120 (MQ=255)
                                                                                                aCTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTa                                                     <  2:376972/149‑1 (MQ=255)
                                                                                                       aCGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCa                                              <  3:175703/149‑1 (MQ=255)
                                                                                                         ggTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATg                                            <  2:654099/149‑1 (MQ=255)
                                                                                                          gTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATgg                                           <  1:324608/149‑1 (MQ=255)
                                                                                                          gTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATgg                                           <  2:27416/149‑1 (MQ=255)
                                                                                                          gTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATgg                                           <  1:475106/149‑1 (MQ=255)
                                                                                                           ttACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATggg                                          >  4:1058497/1‑149 (MQ=255)
                                                                                                           ttACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATggg                                          <  4:23107/149‑1 (MQ=255)
                                                                                                            tactacCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGa                                         <  2:224288/149‑1 (MQ=255)
                                                                                                              ctacCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAgg                                       <  3:766981/149‑1 (MQ=255)
                                                                                                               tacCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAggg                                      >  2:510897/1‑149 (MQ=255)
                                                                                                               tacCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAggg                                      >  4:429857/1‑149 (MQ=255)
                                                                                                                     ggCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTCAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTaa                                                                <  1:751280/117‑1 (MQ=255)
                                                                                                                      gCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTg                               <  2:85563/149‑1 (MQ=255)
                                                                                                                       ccGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTgg                              <  4:368667/149‑1 (MQ=255)
                                                                                                                              gTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTg                               <  1:1053844/141‑1 (MQ=255)
                                                                                                                              gTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTg                               >  2:1053844/1‑141 (MQ=255)
                                                                                                                                      tAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGt               >  4:429560/1‑149 (MQ=255)
                                                                                                                                       aaGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGAttt                                 <  4:301598/130‑1 (MQ=255)
                                                                                                                                       aaGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGAttt                                 >  3:301598/1‑130 (MQ=255)
                                                                                                                                         ttcaTCACGCAGATGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGcc            >  4:322664/4‑149 (MQ=255)
                                                                                                                                         ttcaTCACGCAGATGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGcc            >  2:798903/4‑149 (MQ=255)
                                                                                                                                          tcaTCACGCAGATGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTg                               >  3:1071317/3‑129 (MQ=255)
                                                                                                                                            aTCACGCATCTGCGTGATGAATCTAAGTGTGGATTATCCCCAGGCAACTGGGGAACGACCAAACCGGGCGGCGACGATATCTTGAAACGTCTAACCCTGGCGTTACATGTCACGGGAGGAGATCTTGGCCCTTCCGTGACAGTGCCTgc         >  4:670855/1‑149 (MQ=255)
                                                                                                                                             tCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGt                                                >  1:157238/1‑109 (MQ=255)
                                                                                                                                              cACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTgcag       <  4:888140/149‑1 (MQ=255)
                                                                                                                                               aCGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTgcagc      >  3:588910/1‑149 (MQ=255)
                                                                                                                                               aCGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTgcagc      >  3:482240/1‑149 (MQ=255)
                                                                                                                                               aCGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTgcaat      >  4:379759/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTG  >  NC_000913/1147400‑1147695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: