Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,625,976 A→C 27.0% F469V (TTC→GTC)  dcp ← dipeptidyl carboxypeptidase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,625,9760AC27.0% 191.8 / 106.3 115F469V (TTC→GTC) dcpdipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand):  ref base A (37/47);  new base C (17/14);  total (54/61)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

Only 100 of 116 total aligned reads displayed.

GCGAGCGAATACCTGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAA  >  NC_000913/1625830‑1626124
                                                                                                                                                  |                                                                                                                                                    
gcgagcgaATACCTGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATa                                                                                                                                                    >  3:46996/1‑149 (MQ=255)
  gagcgaATACCTGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAg                                                                                                                                                  <  1:786083/149‑1 (MQ=255)
  gagcgaATACCTGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAg                                                                                                                                                  <  1:362938/149‑1 (MQ=255)
  caggcaaTACCTGCGGATGTCTTGCCCAATGTTCGGTTAATTTCCACGGGAAATCGAAAAAATCACGGGGTGTGTTTGTGCCCGAAAGCCTTGTATTAAGGTGGGGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAg                                                                                                                                                  <  2:326163/144‑1 (MQ=255)
   agcgaATACCTGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAgg                                                                                                                                                 <  3:900374/149‑1 (MQ=255)
     cgaATACCTGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGtt                                                                                                                                               <  3:1038522/149‑1 (MQ=255)
      gaATACCTGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTa                                                                                                                                              >  4:90411/1‑149 (MQ=255)
       gaTACCTGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTAt                                                                                                                                             <  2:295318/148‑1 (MQ=255)
        aTACCTGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATg                                                                                                                                            <  4:426859/149‑1 (MQ=255)
            cTGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGAcat                                                                                                                                        >  1:760835/1‑149 (MQ=255)
            cTGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGAcat                                                                                                                                        >  3:747762/1‑149 (MQ=255)
            cTGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGAcat                                                                                                                                        >  1:1026146/1‑149 (MQ=255)
             tGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGAcatc                                                                                                                                       >  3:515135/1‑149 (MQ=255)
             tGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGAcatc                                                                                                                                       >  3:951614/1‑149 (MQ=255)
                 gATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATcc                                                                                                                                   <  3:661559/149‑1 (MQ=255)
                  aTGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATccc                                                                                                                                  <  2:1026146/149‑1 (MQ=255)
                   tGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACTAAATTCATGGAATAAGGTTATGACATCATCCCa                                                                                                                                 <  2:170350/149‑1 (MQ=255)
                      gTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCagag                                                                                                                              >  1:480632/1‑149 (MQ=255)
                      gTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCagag                                                                                                                              >  2:160312/1‑149 (MQ=255)
                         gCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTaa                                                                                                                           >  3:131456/1‑149 (MQ=255)
                           ccAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGAcatc                                                                                                                                       <  3:485118/135‑1 (MQ=255)
                           ccAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGAcatc                                                                                                                                       >  4:485118/1‑135 (MQ=255)
                           ccAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTaaca                                                                                                                         >  1:25530/1‑149 (MQ=255)
                            cAGTGTTCGTTGTTTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTaacaa                                                                                                                        >  3:80893/1‑149 (MQ=255)
                                gtTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCa                                                                                                                    <  3:970574/149‑1 (MQ=255)
                                 ttCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCa                                                                                                                    <  2:515039/148‑1 (MQ=255)
                                 ttCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCa                                                                                                                    >  1:515039/1‑148 (MQ=255)
                                 ttCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAg                                                                                                                   >  4:7795/1‑149 (MQ=255)
                                   cGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCCGAGTAACAACGCAgtc                                                                                                                 <  2:253043/149‑3 (MQ=255)
                                   cGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGc                                                                                                                 <  4:894869/149‑1 (MQ=255)
                                    gTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCt                                                                                                                >  3:525379/1‑149 (MQ=255)
                                     ttGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTc                                                                                                               >  2:222335/1‑149 (MQ=255)
                                     ttGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTc                                                                                                               >  3:957205/1‑149 (MQ=255)
                                         tttGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCg                                                                                                           >  1:974776/1‑149 (MQ=255)
                                               aCGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCaga                                                                                                                               <  1:719943/123‑1 (MQ=255)
                                               aCGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCaga                                                                                                                               >  2:719943/1‑123 (MQ=255)
                                                cGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCgg                                                                                                    <  3:866263/149‑1 (MQ=255)
                                                 ggAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCggg                                                                                                   >  1:1035929/1‑149 (MQ=255)
                                                     attctgcAAAATCACGCGGCGTGTAGGGGCCGGAAAGTGTGGCATATCGCGGGCGGGCAAAAAGGCCGTGCAGCGTATGACAAAATTCATGGAATAAGCTTATGACATCAGCCCAGAGTAACAACGCAGGCTCACCGGGAGCGGGtttt                                                                                               <  2:319213/143‑2 (MQ=255)
                                                     agtCGACAAAATCACGCGGCGTGTTGGTGACGGAAAGCGTGGCATAAAGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCTGCGTAACAACTCAGCCTCACCGGCAGCGGGTTTc                                                                                               <  2:488246/147‑1 (MQ=255)
                                                     actCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCACATTCATGGAATAAGGTTATGACATCATCCCAGAGCAACAACGCAGGCTCACCGGCCGCGGGTTTc                                                                                               <  2:766403/147‑1 (MQ=255)
                                                     aTTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAAGGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTc                                                                                               <  3:679194/149‑1 (MQ=255)
                                                      ttCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCt                                                                                              >  3:839103/1‑149 (MQ=255)
                                                        cGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGa                                                                                            <  4:652897/149‑1 (MQ=255)
                                                         gACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGAt                                                                                           >  3:295321/1‑149 (MQ=255)
                                                         gACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGAt                                                                                           >  3:107912/1‑149 (MQ=255)
                                                          aCAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCATGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATa                                                                                          >  2:811932/1‑149 (MQ=255)
                                                           cAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATaa                                                                                         >  4:322981/1‑149 (MQ=255)
                                                           cAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATaa                                                                                         <  3:90411/149‑1 (MQ=255)
                                                           cAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATaa                                                                                         <  2:515093/149‑1 (MQ=255)
                                                           cAAAATCACGCGGCGTGTTGGTGCAGCAAAGCGTGGCATAAAGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGATCAAATTCATGGACTAAGGTTATGACATCATCCCAAAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATaa                                                                                         <  1:1075885/149‑1 (MQ=255)
                                                            aaaaTCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGt                                                                                                                             <  4:64881/112‑1 (MQ=255)
                                                            aaaaTCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGt                                                                                                                             >  3:64881/1‑112 (MQ=255)
                                                              aaTCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTg                                                                                      >  4:480251/1‑149 (MQ=255)
                                                               aTCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGc                                                                                     >  1:822091/1‑149 (MQ=255)
                                                               aTCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGc                                                                                     >  4:326461/1‑149 (MQ=255)
                                                               aTCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGc                                                                                     >  2:252156/1‑149 (MQ=255)
                                                                  aCGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGGGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGa                                                                                  <  2:1012501/149‑1 (MQ=255)
                                                                     cggcgTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGt                                                                               <  4:951614/149‑1 (MQ=255)
                                                                       gcgTGTTGGTGCCGGAAAGCGTCGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTCCAGACGTta                                                                             <  1:947757/149‑1 (MQ=255)
                                                                          tgtTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCaga                                                                                                                               <  4:8151/96‑1 (MQ=255)
                                                                          tgtTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCaga                                                                                                                               >  3:8151/1‑96 (MQ=255)
                                                                           gtTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATaaa                                                                         <  4:107912/149‑1 (MQ=255)
                                                                               gTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTAc                                                                     <  1:160312/149‑1 (MQ=255)
                                                                               gTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTAc                                                                     >  1:134746/1‑149 (MQ=255)
                                                                               gTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTAc                                                                     <  3:74814/149‑1 (MQ=255)
                                                                                    ggAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGAt                                                                >  3:303141/1‑149 (MQ=255)
                                                                                         gCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGtt                                                           <  2:706043/149‑1 (MQ=255)
                                                                                          cGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGttt                                                          <  3:247974/149‑1 (MQ=255)
                                                                                           gTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCg                                                                   >  1:1053123/1‑139 (MQ=255)
                                                                                           gTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCg                                                                   <  2:1053123/139‑1 (MQ=255)
                                                                                            tGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTtta                                                        <  3:283995/149‑1 (MQ=255)
                                                                                            tGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTtta                                                        <  3:1080485/149‑1 (MQ=255)
                                                                                                aTAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTaa                                                    <  2:760835/149‑1 (MQ=255)
                                                                                                aTAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTaa                                                    <  4:332731/149‑1 (MQ=255)
                                                                                                 tAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAg                                                   >  1:1081734/1‑149 (MQ=255)
                                                                                                  aaCGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACTACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGc                                                  >  2:785057/1‑149 (MQ=255)
                                                                                                  aaCGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATCGCAGACGTTATAAATTACCGGATGTGTTtta                                                        >  4:967010/1‑143 (MQ=255)
                                                                                                  aaCGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATCGCAGACGTTATAAATTACCGGATGTGTTtta                                                        <  3:967010/143‑1 (MQ=255)
                                                                                                    cGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGt                                                >  4:241090/1‑149 (MQ=255)
                                                                                                       tGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGa                                             <  2:1072288/149‑1 (MQ=255)
                                                                                                       tGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGa                                             <  2:25530/149‑1 (MQ=255)
                                                                                                          cGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTg                                          <  2:974776/149‑1 (MQ=255)
                                                                                                             gCAAAAAAGCCGTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTc                                       >  2:592748/1‑149 (MQ=255)
                                                                                                                 aaaGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTcaaca                                   >  1:133864/1‑149 (MQ=255)
                                                                                                                    ggCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACaaaa                                >  1:463862/1‑149 (MQ=255)
                                                                                                                    ggCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACaaaa                                >  4:931682/1‑149 (MQ=255)
                                                                                                                      ccGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAAtt                              <  3:326461/149‑1 (MQ=255)
                                                                                                                        gTGCAGCGTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGc                            >  2:400057/1‑149 (MQ=255)
                                                                                                                               gTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTCTTAAGCGTTGa                                             <  3:698887/125‑1 (MQ=255)
                                                                                                                               gTATGACCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGa                                             >  4:698887/1‑125 (MQ=255)
                                                                                                                                    aCCAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTAc                                                                     >  2:97598/1‑96 (MQ=255)
                                                                                                                                     ccAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCAcc               >  3:962501/1‑149 (MQ=255)
                                                                                                                                      cAAATTCATGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCAccg              >  4:61854/1‑149 (MQ=255)
                                                                                                                                            cATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCtt        <  2:1009659/149‑1 (MQ=255)
                                                                                                                                             aTGGACTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCttt       >  2:921274/1‑149 (MQ=255)
                                                                                                                                             aTGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCttt       >  3:518182/1‑149 (MQ=255)
                                                                                                                                             aTGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCttt       >  2:84154/1‑149 (MQ=255)
                                                                                                                                               ggAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGAATGCTCAACAAAATTCCCCACCTACGCGCCGCTCCtttga     <  4:636675/149‑3 (MQ=255)
                                                                                                                                                  aTAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGaa  <  2:104350/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCGAGCGAATACCTGCGGATGCGTTGCCCAGTGTTCGTTGATTTGCGACGGAAATTCGACAAAATCACGCGGCGTGTTGGTGCCGGAAAGCGTGGCATAACGCTGGCGGGCAAAAAGGCCGTGCAGCGTATGACCAAATTCATGGAATAAGGTTATGACATCATCCCAGAGTAACAACGCAGGCTCACCGGCAGCGGGTTTCTGATAATTGCAGACGTTATAAATTACCGGATGTGTTTTATTAAGCGTTGATTGCTCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAA  >  NC_000913/1625830‑1626124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: