Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,660,438 A→G 5.3% S790S (TCA→TCG ynfE → putative selenate reductase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,660,4380AG 5.3% 427.5 / 9.4 132S790S (TCA→TCGynfEputative selenate reductase, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (57/68);  new base G (3/4);  total (60/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.35e-01

Only 100 of 140 total aligned reads displayed.

GCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGA  >  NC_000913/1660290‑1660586
                                                                                                                                                    |                                                                                                                                                    
  gAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTcgcc                                                                                                                                                    >  1:725443/1‑149 (MQ=255)
  gAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTcacc                                                                                                                                                    >  4:99247/1‑149 (MQ=255)
     gTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACt                                                                                                                                                 >  1:514329/1‑149 (MQ=255)
      ttCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTg                                                                                                                                                <  4:394761/149‑1 (MQ=255)
        cGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGc                                                                                                                                              >  4:241203/1‑149 (MQ=255)
           cTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATTAAATCGACCATGTCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGaa                                                                                                                                           <  4:793126/149‑1 (MQ=255)
            tACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAg                                                                                                                                          <  2:725443/149‑1 (MQ=255)
            tACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAg                                                                                                                                          <  2:337041/149‑1 (MQ=255)
            tACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAg                                                                                                                                          <  1:138869/149‑1 (MQ=255)
            tACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAg                                                                                                                                          <  3:421272/149‑1 (MQ=255)
             aCCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAgg                                                                                                                                         <  3:714734/149‑1 (MQ=255)
             aCCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAgg                                                                                                                                         <  3:9068/149‑1 (MQ=255)
               cAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGa                                                                                                                                       <  4:337267/149‑1 (MQ=255)
                aGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaa                                                                                                                                      >  1:298111/1‑149 (MQ=255)
                 gCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa                                                                                                                                     >  2:642440/1‑149 (MQ=255)
                 gCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa                                                                                                                                     >  1:380735/1‑149 (MQ=255)
                 gCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa                                                                                                                                     >  4:847996/1‑149 (MQ=255)
                  ccAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAAc                                                                                                                                    >  1:466898/1‑149 (MQ=255)
                    aaaGTAACCCCGCGTAATCACCCAGGGATAAGAGCTATGGGCAAAGTAGCCTTGTACGAGACAAATAAGTCAGGCGATACAATCAACAATGGCGGATGTGTGAATACTCTAACCACTCTGCGCCCTTCACCGCCGGCGTAGGGatgccc                                                                                                                                  <  2:176948/149‑6 (MQ=255)
                      aGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAGGGGAAACCCgc                                                                                                                                <  3:982787/149‑1 (MQ=255)
                       gTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgca                                                                                                                               <  2:466898/149‑1 (MQ=255)
                          acacCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgcagca                                                                                                                            >  1:675415/1‑149 (MQ=255)
                              cacGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACt                                                                                                                        >  3:417396/1‑149 (MQ=255)
                              cacGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCCAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACt                                                                                                                        <  4:205366/149‑1 (MQ=255)
                                   aTTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCt                                                                                                                   >  4:443555/1‑149 (MQ=255)
                                      ctcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGcac                                                                                                                           >  1:887190/1‑138 (MQ=255)
                                      ctcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGcac                                                                                                                           <  2:887190/138‑1 (MQ=255)
                                      ctcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGt                                                                                                                <  2:298111/149‑1 (MQ=255)
                                       tcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTc                                                                                                               >  4:747932/1‑149 (MQ=255)
                                       tcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTc                                                                                                               >  4:709530/1‑149 (MQ=255)
                                          cTGGAGTTCGCGCTATGGGTCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAg                                                                                                                                          >  3:998160/1‑119 (MQ=255)
                                           tGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCgaga                                                                                                           <  4:1046493/149‑1 (MQ=255)
                                            ggAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGAt                                                                                                          <  2:951055/149‑1 (MQ=255)
                                               gTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGa                                                                                                       >  4:975512/1‑149 (MQ=255)
                                                    cgcTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa                                                                                                  <  4:145222/149‑1 (MQ=255)
                                                      cTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAata                                                                                                >  2:475453/1‑149 (MQ=255)
                                                       tATGGGCCAGGGAGCCTGGCACGAGGCCAATAAGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACAACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAatat                                                                                               >  4:133280/1‑149 (MQ=255)
                                                            gCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAccc                                                                                          >  3:363371/1‑149 (MQ=255)
                                                              cAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCAc                                                                                        >  1:566612/1‑149 (MQ=255)
                                                               aGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAc                                                                                            <  4:1054971/144‑1 (MQ=255)
                                                               aGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAc                                                                                            >  3:1054971/1‑144 (MQ=255)
                                                                   aGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACaa                                                                                   <  3:98603/149‑1 (MQ=255)
                                                                      cTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa                                                                                >  2:579039/1‑149 (MQ=255)
                                                                       tGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCAt                                                                               >  2:824140/1‑149 (MQ=255)
                                                                        ggCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATa                                                                              >  3:268910/1‑149 (MQ=255)
                                                                          cACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATaaa                                                                            <  3:971474/149‑1 (MQ=255)
                                                                              aGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCt                                                                        <  2:843951/149‑1 (MQ=255)
                                                                                gCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTgg                                                                      <  3:576241/149‑1 (MQ=255)
                                                                                  cAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCa                                                                    >  1:168575/1‑149 (MQ=255)
                                                                                        gTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTca                                                                                                                                                      <  4:354526/61‑1 (MQ=255)
                                                                                        gTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTca                                                                                                                                                      >  3:354526/1‑61 (MQ=255)
                                                                                             gCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgt                                                         <  3:1013767/149‑1 (MQ=255)
                                                                                              cGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtt                                                        <  3:799465/149‑1 (MQ=255)
                                                                                              cGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtt                                                        <  3:201546/149‑1 (MQ=255)
                                                                                               gATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgttt                                                       >  3:141905/1‑149 (MQ=255)
                                                                                                aTAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtttg                                                      <  3:241203/149‑1 (MQ=255)
                                                                                                aTAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtttg                                                      >  2:889268/1‑149 (MQ=255)
                                                                                                  aaaaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCCCACTAATCTGGGCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTTaa                                                    >  1:255061/1‑149 (MQ=255)
                                                                                                   aaaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaa                                                   <  1:434296/149‑1 (MQ=255)
                                                                                                    aaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaaa                                                  <  4:1075645/149‑1 (MQ=255)
                                                                                                    aaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaaa                                                  <  3:145271/149‑1 (MQ=255)
                                                                                                    aaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaaa                                                  >  2:131663/1‑149 (MQ=255)
                                                                                                    aaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaaa                                                  >  2:851177/1‑149 (MQ=255)
                                                                                                     aTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAg                                                 <  3:815514/149‑1 (MQ=255)
                                                                                                     aTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa                                                                                                                                     >  3:344125/1‑65 (MQ=255)
                                                                                                     aTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa                                                                                                                                     <  4:344125/65‑1 (MQ=255)
                                                                                                     aTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAg                                                 >  4:185054/1‑149 (MQ=255)
                                                                                                       cGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCa                                               <  1:851177/149‑1 (MQ=255)
                                                                                                          ccATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATa                                            >  4:756044/1‑149 (MQ=255)
                                                                                                           cATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATaa                                           <  3:1067472/149‑1 (MQ=255)
                                                                                                             tGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCACAGCCCACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAGTTTGGTTGAAAAGCAATAAGt                                         <  2:977216/149‑1 (MQ=255)
                                                                                                              ggcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCaa                                              >  2:339725/1‑143 (MQ=255)
                                                                                                              ggcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCaa                                              <  1:339725/143‑1 (MQ=255)
                                                                                                               gcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGa                                       <  3:1048913/149‑1 (MQ=255)
                                                                                                                 ggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGa                                       >  3:676834/1‑147 (MQ=255)
                                                                                                                 ggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGa                                       <  4:1028398/147‑1 (MQ=255)
                                                                                                                 ggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGa                                       <  4:676834/147‑1 (MQ=255)
                                                                                                                    tgtgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAat                                  >  2:189405/1‑149 (MQ=255)
                                                                                                                      tgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATATGTGAGTAatga                                <  3:847996/149‑1 (MQ=255)
                                                                                                                      tgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAatga                                <  1:1073260/149‑1 (MQ=255)
                                                                                                                          aaTACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAGTTCTGGCATGACATTTTGTTTGAAAAGCAATAAGt                                         <  1:515751/136‑1 (MQ=255)
                                                                                                                            tACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgt                                                         <  4:755519/118‑1 (MQ=255)
                                                                                                                            tACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCAAAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGaaaa                          <  4:127523/149‑1 (MQ=255)
                                                                                                                               gCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGt                                         <  3:239427/131‑1 (MQ=255)
                                                                                                                                cTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCa                      <  1:357090/149‑1 (MQ=255)
                                                                                                                                  aaCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATa                    <  1:663081/149‑1 (MQ=255)
                                                                                                                                   aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc                   >  4:249975/1‑149 (MQ=255)
                                                                                                                                   aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc                   <  2:566612/149‑1 (MQ=255)
                                                                                                                                   aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc                   <  2:43037/149‑1 (MQ=255)
                                                                                                                                      aCTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACcac                >  3:772/1‑149 (MQ=255)
                                                                                                                                        tctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAg                                      <  2:76643/125‑1 (MQ=255)
                                                                                                                                        tctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAg                                      >  1:76643/1‑125 (MQ=255)
                                                                                                                                        tctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACag              >  2:106054/1‑149 (MQ=255)
                                                                                                                                          tGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTACTCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGAGAGTAATGATGAAAATCCATACCACagag            >  2:910920/1‑149 (MQ=255)
                                                                                                                                           tttcccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa                                                                                <  4:876275/77‑1 (MQ=38)
                                                                                                                                           tttcccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa                                                                                >  3:876275/4‑80 (MQ=38)
                                                                                                                                            ttcccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtt                                                        >  3:256658/3‑103 (MQ=255)
                                                                                                                                            cgcCCTTCACGACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGgc          <  2:431460/149‑1 (MQ=255)
                                                                                                                                              cccTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTAGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGgcgc        >  3:1003232/1‑149 (MQ=255)
                                                                                                                                                    accacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGa  >  4:234051/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGA  >  NC_000913/1660290‑1660586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: