Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,660,438 | A→G | 5.3% | S790S (TCA→TCG) | ynfE → | putative selenate reductase, periplasmic |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,660,438 | 0 | A | G | 5.3% | 427.5 / 9.4 | 132 | S790S (TCA→TCG) | ynfE | putative selenate reductase, periplasmic |
Reads supporting (aligned to +/- strand): ref base A (57/68); new base G (3/4); total (60/72) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.35e-01 |
Only 100 of 140 total aligned reads displayed.
GCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGA > NC_000913/1660290‑1660586 | gAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTcgcc > 1:725443/1‑149 (MQ=255) gAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTcacc > 4:99247/1‑149 (MQ=255) gTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACt > 1:514329/1‑149 (MQ=255) ttCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTg < 4:394761/149‑1 (MQ=255) cGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGc > 4:241203/1‑149 (MQ=255) cTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATTAAATCGACCATGTCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGaa < 4:793126/149‑1 (MQ=255) tACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAg < 2:725443/149‑1 (MQ=255) tACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAg < 2:337041/149‑1 (MQ=255) tACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAg < 1:138869/149‑1 (MQ=255) tACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAg < 3:421272/149‑1 (MQ=255) aCCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAgg < 3:714734/149‑1 (MQ=255) aCCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAgg < 3:9068/149‑1 (MQ=255) cAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGa < 4:337267/149‑1 (MQ=255) aGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaa > 1:298111/1‑149 (MQ=255) gCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa > 2:642440/1‑149 (MQ=255) gCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa > 1:380735/1‑149 (MQ=255) gCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa > 4:847996/1‑149 (MQ=255) ccAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAAc > 1:466898/1‑149 (MQ=255) aaaGTAACCCCGCGTAATCACCCAGGGATAAGAGCTATGGGCAAAGTAGCCTTGTACGAGACAAATAAGTCAGGCGATACAATCAACAATGGCGGATGTGTGAATACTCTAACCACTCTGCGCCCTTCACCGCCGGCGTAGGGatgccc < 2:176948/149‑6 (MQ=255) aGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAGGGGAAACCCgc < 3:982787/149‑1 (MQ=255) gTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgca < 2:466898/149‑1 (MQ=255) acacCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgcagca > 1:675415/1‑149 (MQ=255) cacGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACt > 3:417396/1‑149 (MQ=255) cacGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCCAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACt < 4:205366/149‑1 (MQ=255) aTTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCt > 4:443555/1‑149 (MQ=255) ctcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGcac > 1:887190/1‑138 (MQ=255) ctcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGcac < 2:887190/138‑1 (MQ=255) ctcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGt < 2:298111/149‑1 (MQ=255) tcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTc > 4:747932/1‑149 (MQ=255) tcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTc > 4:709530/1‑149 (MQ=255) cTGGAGTTCGCGCTATGGGTCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAg > 3:998160/1‑119 (MQ=255) tGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCgaga < 4:1046493/149‑1 (MQ=255) ggAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGAt < 2:951055/149‑1 (MQ=255) gTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGa > 4:975512/1‑149 (MQ=255) cgcTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa < 4:145222/149‑1 (MQ=255) cTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAata > 2:475453/1‑149 (MQ=255) tATGGGCCAGGGAGCCTGGCACGAGGCCAATAAGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACAACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAatat > 4:133280/1‑149 (MQ=255) gCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAccc > 3:363371/1‑149 (MQ=255) cAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCAc > 1:566612/1‑149 (MQ=255) aGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAc < 4:1054971/144‑1 (MQ=255) aGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAc > 3:1054971/1‑144 (MQ=255) aGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACaa < 3:98603/149‑1 (MQ=255) cTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa > 2:579039/1‑149 (MQ=255) tGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCAt > 2:824140/1‑149 (MQ=255) ggCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATa > 3:268910/1‑149 (MQ=255) cACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATaaa < 3:971474/149‑1 (MQ=255) aGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCt < 2:843951/149‑1 (MQ=255) gCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTgg < 3:576241/149‑1 (MQ=255) cAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCa > 1:168575/1‑149 (MQ=255) gTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTca < 4:354526/61‑1 (MQ=255) gTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTca > 3:354526/1‑61 (MQ=255) gCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgt < 3:1013767/149‑1 (MQ=255) cGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtt < 3:799465/149‑1 (MQ=255) cGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtt < 3:201546/149‑1 (MQ=255) gATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgttt > 3:141905/1‑149 (MQ=255) aTAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtttg < 3:241203/149‑1 (MQ=255) aTAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtttg > 2:889268/1‑149 (MQ=255) aaaaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCCCACTAATCTGGGCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTTaa > 1:255061/1‑149 (MQ=255) aaaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaa < 1:434296/149‑1 (MQ=255) aaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaaa < 4:1075645/149‑1 (MQ=255) aaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaaa < 3:145271/149‑1 (MQ=255) aaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaaa > 2:131663/1‑149 (MQ=255) aaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaaa > 2:851177/1‑149 (MQ=255) aTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAg < 3:815514/149‑1 (MQ=255) aTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa > 3:344125/1‑65 (MQ=255) aTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa < 4:344125/65‑1 (MQ=255) aTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAg > 4:185054/1‑149 (MQ=255) cGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCa < 1:851177/149‑1 (MQ=255) ccATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATa > 4:756044/1‑149 (MQ=255) cATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATaa < 3:1067472/149‑1 (MQ=255) tGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCACAGCCCACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAGTTTGGTTGAAAAGCAATAAGt < 2:977216/149‑1 (MQ=255) ggcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCaa > 2:339725/1‑143 (MQ=255) ggcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCaa < 1:339725/143‑1 (MQ=255) gcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGa < 3:1048913/149‑1 (MQ=255) ggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGa > 3:676834/1‑147 (MQ=255) ggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGa < 4:1028398/147‑1 (MQ=255) ggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGa < 4:676834/147‑1 (MQ=255) tgtgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAat > 2:189405/1‑149 (MQ=255) tgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATATGTGAGTAatga < 3:847996/149‑1 (MQ=255) tgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAatga < 1:1073260/149‑1 (MQ=255) aaTACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAGTTCTGGCATGACATTTTGTTTGAAAAGCAATAAGt < 1:515751/136‑1 (MQ=255) tACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgt < 4:755519/118‑1 (MQ=255) tACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCAAAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGaaaa < 4:127523/149‑1 (MQ=255) gCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGt < 3:239427/131‑1 (MQ=255) cTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCa < 1:357090/149‑1 (MQ=255) aaCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATa < 1:663081/149‑1 (MQ=255) aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc > 4:249975/1‑149 (MQ=255) aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc < 2:566612/149‑1 (MQ=255) aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc < 2:43037/149‑1 (MQ=255) aCTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACcac > 3:772/1‑149 (MQ=255) tctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAg < 2:76643/125‑1 (MQ=255) tctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAg > 1:76643/1‑125 (MQ=255) tctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACag > 2:106054/1‑149 (MQ=255) tGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTACTCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGAGAGTAATGATGAAAATCCATACCACagag > 2:910920/1‑149 (MQ=255) tttcccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa < 4:876275/77‑1 (MQ=38) tttcccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa > 3:876275/4‑80 (MQ=38) ttcccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtt > 3:256658/3‑103 (MQ=255) cgcCCTTCACGACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGgc < 2:431460/149‑1 (MQ=255) cccTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTAGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGgcgc > 3:1003232/1‑149 (MQ=255) accacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGa > 4:234051/1‑149 (MQ=255) | GCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGA > NC_000913/1660290‑1660586 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |