Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,851,204 A→C 5.7% T115T (ACA→ACC ansA → cytoplasmic L‑asparaginase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,851,2040AC 5.7% 336.2 / 9.0 100T115T (ACA→ACCansAcytoplasmic L‑asparaginase 1
Reads supporting (aligned to +/- strand):  ref base A (50/44);  new base C (3/3);  total (53/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 101 total aligned reads displayed.

TGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCC  >  NC_000913/1851061‑1851352
                                                                                                                                               |                                                                                                                                                    
tGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGAACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTc                                                                                                                                                 <  3:1009906/149‑1 (MQ=255)
  gcagcaTATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGTGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTcac                                                                                                                                               >  4:304162/1‑149 (MQ=255)
    agcaTATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACCCTGTCACaa                                                                                                                                             <  1:970045/149‑1 (MQ=255)
          ttGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACCCTGTCACaatgaccg                                                                                                                                       <  4:286415/149‑7 (MQ=255)
            gCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgct                                                                                                                                     <  1:448067/149‑1 (MQ=255)
              tGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctgg                                                                                                                                   >  4:206100/1‑149 (MQ=255)
              tGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGAGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctgg                                                                                                                                   <  1:535037/149‑1 (MQ=255)
                aaGATATTAAAGCGCACTATGACGACTGTGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACAGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGGCATTGTGACCGGGTCACAAATCCCgctggca                                                                                                                                 >  4:380830/1‑148 (MQ=255)
                  gATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGa                                                                                                                               <  3:138608/149‑1 (MQ=255)
                  gATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGa                                                                                                                               <  3:741963/149‑1 (MQ=255)
                   atatTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAg                                                                                                                              >  2:810009/1‑149 (MQ=255)
                   atatTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAg                                                                                                                              >  4:64588/1‑149 (MQ=255)
                   atatTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAg                                                                                                                              >  3:478843/1‑149 (MQ=255)
                    tatTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGt                                                                                                                             >  4:378557/1‑149 (MQ=255)
                     atTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGtt                                                                                                                            >  1:365021/1‑149 (MQ=255)
                      ttAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTa                                                                                                                           >  3:1076052/1‑149 (MQ=255)
                      ttAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTa                                                                                                                           >  3:575243/1‑149 (MQ=255)
                        aaaGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACg                                                                                                                         <  1:26770/149‑1 (MQ=255)
                                cTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAATTACGCTCTGACg                                                                                                                 >  3:967817/1‑149 (MQ=255)
                                  aTGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGa                                                                                                               <  1:133767/149‑1 (MQ=255)
                                     acgacTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACaa                                                                                                            >  2:437409/1‑149 (MQ=255)
                                       gacTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAAt                                                                                                          >  1:512905/1‑149 (MQ=255)
                                          tATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTaa                                                                                                       >  3:470864/1‑149 (MQ=255)
                                          tATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTaa                                                                                                       >  1:159938/1‑149 (MQ=255)
                                           atgatgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAAt                                                                                                      <  4:1076052/149‑1 (MQ=255)
                                            tgatgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATc                                                                                                     >  3:695733/1‑149 (MQ=255)
                                              atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATctg                                                                                                   >  1:921401/1‑149 (MQ=255)
                                               tgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATctgc                                                                                                  >  3:324842/1‑149 (MQ=255)
                                                ggTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATctgct                                                                                                 <  3:1056465/149‑1 (MQ=255)
                                                  ttttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACCCTGTCACaatga                                                                                                                                          >  4:888769/1‑103 (MQ=255)
                                                    ttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAAt                                                                                             <  2:512905/149‑1 (MQ=255)
                                                       tCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCg                                                                                          <  2:215034/149‑1 (MQ=255)
                                                        cATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGt                                                                                         <  1:437409/149‑1 (MQ=255)
                                                        cATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGt                                                                                         <  3:833620/149‑1 (MQ=255)
                                                         aTTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGtt                                                                                        >  2:712750/1‑149 (MQ=255)
                                                         aTTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGCTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACCCTGTCACaat                                                                                                                                            <  1:713686/97‑2 (MQ=255)
                                                             tGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTAc                                                                                    <  2:228843/149‑1 (MQ=255)
                                                             tGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTAc                                                                                    >  2:708036/1‑149 (MQ=255)
                                                              gCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCAGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACg                                                                                   >  1:541683/1‑149 (MQ=255)
                                                               cACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGt                                                                                  >  1:721790/1‑149 (MQ=255)
                                                                aCGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGtt                                                                                 >  3:209653/1‑149 (MQ=255)
                                                                   gCACCGACACGATGGTGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTgcg                                                                              <  3:304162/149‑1 (MQ=255)
                                                                        gACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGATATGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGt                                                                                         <  3:41349/133‑1 (MQ=255)
                                                                        gACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGt                                                                                         >  4:41349/1‑133 (MQ=255)
                                                                        gACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGaa                                                                         <  3:64588/149‑1 (MQ=255)
                                                                        gACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGaa                                                                         >  2:126461/1‑149 (MQ=255)
                                                                        gACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGaa                                                                         <  2:868127/149‑1 (MQ=255)
                                                                         acacGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAAt                                                                        >  2:564788/1‑149 (MQ=255)
                                                                         acacGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAAt                                                                        >  2:981501/1‑149 (MQ=255)
                                                                             gATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAAt                                                                                             <  1:1036080/124‑1 (MQ=255)
                                                                             gATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAAt                                                                                             >  2:1036080/1‑124 (MQ=255)
                                                                             gATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATc                                                                    <  2:927727/149‑1 (MQ=255)
                                                                              aTGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATcc                                                                   <  2:921401/149‑1 (MQ=255)
                                                                              aTGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATcc                                                                   >  1:783648/1‑149 (MQ=255)
                                                                               tGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCg                                                                  <  1:878245/149‑1 (MQ=255)
                                                                                 gCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGAt                                                                <  2:33610/149‑1 (MQ=255)
                                                                                  cGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgct                                                                                                                                     >  4:677765/1‑79 (MQ=255)
                                                                                  cGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgct                                                                                                                                     <  3:677765/79‑1 (MQ=255)
                                                                                   gTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTg                                                                                       >  1:366238/1‑124 (MQ=255)
                                                                                   gTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTg                                                                                       <  2:366238/124‑1 (MQ=255)
                                                                                         cGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCAGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGCAg                                                        >  2:1075402/1‑149 (MQ=255)
                                                                                           ccTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTa                                                      >  3:1060259/1‑149 (MQ=255)
                                                                                              ctGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACg                                                   <  2:783648/149‑1 (MQ=255)
                                                                                                gcgcTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGct                                                 <  1:647170/149‑1 (MQ=255)
                                                                                                   cTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCtt                                              <  4:710437/149‑1 (MQ=255)
                                                                                                    tGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctggc                                                                                                                                  <  1:200877/64‑1 (MQ=255)
                                                                                                    tGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctggc                                                                                                                                  >  2:200877/1‑64 (MQ=255)
                                                                                                        gTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCa                                         >  3:607324/1‑149 (MQ=255)
                                                                                                             tGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAAc                                    <  2:721790/149‑1 (MQ=255)
                                                                                                              gCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAAcc                                   >  2:216097/1‑149 (MQ=255)
                                                                                                                tCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGa                                 >  2:456019/1‑149 (MQ=255)
                                                                                                                tCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGa                                 >  2:501189/1‑149 (MQ=255)
                                                                                                                tCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGa                                 >  4:517045/1‑149 (MQ=255)
                                                                                                                 cGAGAATCTCGGTAACCCGGTCATTGTGACAGGGTCACAAATCCCGCTAGCTGAGTTACGCTCTGACGGGCAAATTAAGCTGCTGAATGCGTTGTACGTCGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGAt                                <  4:71963/149‑1 (MQ=255)
                                                                                                                 cGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGAt                                <  2:310395/149‑1 (MQ=255)
                                                                                                                  gagaATCTCGGTAAACCGGTCATTGTGACCCTGTCACaat                                                                                                                                            >  2:1068107/1‑39 (MQ=255)
                                                                                                                  gagaATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGTGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGAtt                               <  1:712750/149‑1 (MQ=255)
                                                                                                                  gagaATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGAtt                               <  3:147620/149‑1 (MQ=255)
                                                                                                                  gagaATCTCGGAAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGAtt                               >  3:632323/1‑149 (MQ=255)
                                                                                                                       tctcGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGt                                                                                         <  2:31498/86‑1 (MQ=255)
                                                                                                                       tctcGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGt                                                                                         >  1:31498/1‑86 (MQ=255)
                                                                                                                         tcGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcgc                        <  1:207108/149‑1 (MQ=255)
                                                                                                                         tcGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcgc                        >  3:82799/1‑149 (MQ=255)
                                                                                                                         tcGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAACTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcgc                        <  1:467290/149‑1 (MQ=255)
                                                                                                                           ggTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCgg                      >  3:924854/1‑149 (MQ=255)
                                                                                                                            gTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTTTATCGCGGc                     >  4:476556/1‑149 (MQ=255)
                                                                                                                                aCCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAAcc                 >  3:280373/1‑149 (MQ=255)
                                                                                                                                 ccGGTCATTGTGACAGGGTCACAAATCCCTCTGGCTGAGTTACGCTCTGACGGACAAATTACTCTGCTTAATGCGTTGTACGCTGCGGCGAATCATCCGCTCAACGCAGTAACGCTCTTTTTCAATAACCGACTGTATCGCGGCAACCg                >  4:550310/1‑149 (MQ=255)
                                                                                                                                 ccGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTaa                                                                                                       <  1:368979/62‑1 (MQ=255)
                                                                                                                                 ccGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTaa                                                                                                       >  2:368979/1‑62 (MQ=255)
                                                                                                                                   ggTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCa              >  1:138114/1‑149 (MQ=255)
                                                                                                                                    gTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCAc             <  4:324842/149‑1 (MQ=255)
                                                                                                                                    gTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCAc             <  4:1029396/149‑1 (MQ=255)
                                                                                                                                    gTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCAc             <  3:994251/149‑1 (MQ=255)
                                                                                                                                       aTTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGtt                                                                                        <  4:244742/71‑1 (MQ=255)
                                                                                                                                       aTTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGtt                                                                                        >  3:244742/1‑71 (MQ=255)
                                                                                                                                       aTTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTAc          <  1:307848/149‑1 (MQ=255)
                                                                                                                                       aTTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTAc          >  2:444044/1‑149 (MQ=255)
                                                                                                                                             aCAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGc    <  4:815383/149‑1 (MQ=255)
                                                                                                                                               aGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGccc  >  4:671740/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCC  >  NC_000913/1851061‑1851352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: