Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,865,682 G→C 11.2% intergenic (‑46/+44) yeaE ← / ← mipA aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,865,6820GC11.2% 487.4 / 48.4 154intergenic (‑46/+44)yeaE/mipAaldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
Reads supporting (aligned to +/- strand):  ref base G (63/73);  new base C (8/9);  total (72/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.07e-01

Only 100 of 159 total aligned reads displayed.

CTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACC  >  NC_000913/1865534‑1865830
                                                                                                                                                    |                                                                                                                                                    
cTTTTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTg                                                                                                                                                      <  4:361622/149‑1 (MQ=255)
 ttCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGa                                                                                                                                                     >  4:661324/1‑149 (MQ=255)
  tCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGAc                                                                                                                                                    <  3:339403/149‑1 (MQ=255)
    tGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGc                                                                                                                                                  >  2:683218/1‑149 (MQ=255)
     gTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGcc                                                                                                                                                 <  4:955794/149‑1 (MQ=255)
      ttttGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGccc                                                                                                                                                <  2:982479/149‑1 (MQ=255)
      ttttGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGccc                                                                                                                                                >  1:126459/1‑149 (MQ=255)
      ttttGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGccc                                                                                                                                                <  2:761748/149‑1 (MQ=255)
          gcgcTGACTGGCATCTTCGCCCATATGCCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTCGATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGtt                                                                                                                                            <  2:557638/149‑1 (MQ=255)
          gcgcTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtt                                                                                                                                            <  1:860429/149‑1 (MQ=255)
          gcgcTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtt                                                                                                                                            <  1:970482/149‑1 (MQ=255)
           cgcTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGttt                                                                                                                                           <  4:371712/149‑1 (MQ=255)
           cgcTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttt                                                                                                                                           <  2:635969/149‑1 (MQ=255)
            gcTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtttt                                                                                                                                          <  3:329276/149‑1 (MQ=255)
              tGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTa                                                                                                                                        <  3:389412/149‑1 (MQ=255)
              tGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTa                                                                                                                                        <  3:346875/149‑1 (MQ=255)
                 cTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGc                                                                                                                                     >  4:141142/1‑149 (MQ=255)
                  tGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGca                                                                                                                                    >  4:469138/1‑149 (MQ=255)
                    gCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGcaca                                                                                                                                  >  4:908539/1‑149 (MQ=255)
                     cATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaa                                                                                                                                 >  4:1013275/1‑149 (MQ=255)
                         ttCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATg                                                                                                                             >  2:1082017/1‑149 (MQ=255)
                         ttCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATg                                                                                                                             >  2:304672/1‑149 (MQ=255)
                         ttCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATg                                                                                                                             >  2:1027644/1‑149 (MQ=255)
                            gCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTATTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGccc                                                                                                                          >  4:622237/1‑149 (MQ=255)
                            cgccATATTCCCTGTTCCCTGCCCTTCGGCTGGCAGTGAGACCTCGCCACCTAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGccc                                                                                                                          <  3:741107/147‑1 (MQ=255)
                             cccATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCt                                                                                                                         >  1:204325/1‑149 (MQ=255)
                              ccATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACTGTAATGCAAAACAGGGCGTGACGCCCTGttt                                                                                                                                           >  2:667442/1‑130 (MQ=255)
                              ccATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGttt                                                                                                                                           <  1:667442/130‑1 (MQ=255)
                               cATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTgg                                                                                                                       >  1:440668/1‑149 (MQ=255)
                                     ccATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtt                                                                                                                                            >  3:200611/1‑122 (MQ=255)
                                     ccATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtt                                                                                                                                            <  4:200611/122‑1 (MQ=255)
                                      cATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATg                                                                                                                >  3:947268/1‑149 (MQ=255)
                                       aTGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGc                                                                                                               >  4:859272/1‑149 (MQ=255)
                                        tGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCa                                                                                                              >  2:108578/1‑149 (MQ=255)
                                             ccTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATc                                                                                                         >  4:699017/1‑149 (MQ=255)
                                                 cccTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGaa                                                                                                     <  1:1027644/149‑1 (MQ=255)
                                                  ccTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAt                                                                                                    >  2:251454/1‑149 (MQ=255)
                                                   cTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAtt                                                                                                   >  4:758640/1‑149 (MQ=255)
                                                      cGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGt                                                                                                <  3:212372/149‑1 (MQ=255)
                                                       ggCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTa                                                                                               <  3:110971/149‑1 (MQ=255)
                                                       ggCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTa                                                                                               <  1:1082017/149‑1 (MQ=255)
                                                        gCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAg                                                                                              <  1:1079003/149‑1 (MQ=255)
                                                          tGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGt                                                                                            <  3:924576/149‑1 (MQ=255)
                                                              aGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATc                                                                                        >  2:61868/1‑149 (MQ=255)
                                                              aGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATc                                                                                        >  4:181084/1‑149 (MQ=255)
                                                                  agaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTa                                                                                                                                        <  1:477454/97‑1 (MQ=255)
                                                                  agaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTa                                                                                                                                        >  2:477454/1‑97 (MQ=255)
                                                                  agaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCgg                                                                                    <  1:168090/149‑1 (MQ=255)
                                                                    aCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAg                                                                                              <  1:1103618/137‑1 (MQ=255)
                                                                       tCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaa                                                                               <  3:765985/149‑1 (MQ=255)
                                                                        cGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaaa                                                                              <  2:204325/149‑1 (MQ=255)
                                                                         gCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGt                                                                                            >  3:1028643/1‑134 (MQ=255)
                                                                         gCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGt                                                                                            <  4:1028643/134‑1 (MQ=255)
                                                                            aCTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAg                                                                          >  3:166126/1‑149 (MQ=255)
                                                                            aCTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAg                                                                          >  2:158674/1‑149 (MQ=255)
                                                                             cTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAgg                                                                         >  3:106626/1‑149 (MQ=255)
                                                                              tAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGTAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                                                                        <  2:937151/149‑1 (MQ=255)
                                                                              tAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                                                                        <  3:528737/149‑1 (MQ=255)
                                                                                  ttGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGt                                                                    >  1:625730/1‑149 (MQ=255)
                                                                                     aaTCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGaa                                                                                                     <  3:905030/113‑1 (MQ=255)
                                                                                     aaTCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCa                                                                 <  2:749494/149‑1 (MQ=255)
                                                                                      aTCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCTTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAg                                                                <  4:287835/149‑1 (MQ=255)
                                                                                       tCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAgg                                                               >  4:523208/1‑149 (MQ=255)
                                                                                       tCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAgg                                                               >  2:144411/1‑149 (MQ=255)
                                                                                         aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGa                                                              >  3:123508/1‑148 (MQ=255)
                                                                                         aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGa                                                              <  4:123508/148‑1 (MQ=255)
                                                                                         aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCATGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCACTCCAGGAt                                                             >  2:171553/1‑149 (MQ=255)
                                                                                          ttttttGTTGCGTTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAtt                                                            >  4:872948/1‑149 (MQ=255)
                                                                                          ttttttGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATCCACAAAATGCCCTGGAAAGATGCATTATCAGAACTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAtt                                                            >  1:736333/1‑149 (MQ=255)
                                                                                          ttttttGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTAACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAtt                                                            >  3:578844/1‑149 (MQ=255)
                                                                                             tttCTTGCAGTGTTTCCAACTAGGTATTGCACCACCGCAATGCCAAACTAGGCTTGACGCCCTGTTTTTATGCACAAAATGCACTGTCAATATGCATTATAAGAATTTATCAGTGACACCGTCAGATATTATGCCAGTCAAAGATTTAt                                                         <  4:926802/149‑1 (MQ=255)
                                                                                               tgttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATcc                                                       <  4:486324/149‑1 (MQ=255)
                                                                                                 ttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATCCACAAAATGCCCTGGAAAGACGCCTTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCAAGTCCAGGATTTATccac                                                     >  2:639523/1‑149 (MQ=255)
                                                                                                     aTTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATc                                                 <  1:325579/149‑1 (MQ=255)
                                                                                                     aTTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATc                                                 >  2:712384/1‑149 (MQ=255)
                                                                                                       tGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAg                                                                          <  1:1527/122‑1 (MQ=255)
                                                                                                       tGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCgg                                               <  4:966608/149‑1 (MQ=255)
                                                                                                          ttCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCt                                            <  1:362009/149‑1 (MQ=255)
                                                                                                             ctccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTc                                         >  4:958347/1‑149 (MQ=255)
                                                                                                                ccAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCATGGAAAGAAGCATTATCAGCATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGt                                      >  2:574989/1‑149 (MQ=255)
                                                                                                                 cAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTAt                                                         >  3:367581/1‑129 (MQ=255)
                                                                                                                  aGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTaa                                    <  3:908539/149‑1 (MQ=255)
                                                                                                                      aTTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtc                                <  4:336885/149‑1 (MQ=255)
                                                                                                                           accaccGTAATGCAAGACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTaa                                    >  4:797195/1‑140 (MQ=255)
                                                                                                                           accaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTaa                                    <  3:797195/140‑1 (MQ=255)
                                                                                                                           accaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATACACCATCGGGCTGTCAGTAACTTCATcag                           >  3:1021188/1‑149 (MQ=255)
                                                                                                                            ccaccGGAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGTCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAAGCAGGCCAGTCCAGGATTTATCAACCATAGGTCTGTCAGTAGCTTCATcaga                          <  2:793794/149‑1 (MQ=255)
                                                                                                                             caccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagac                         >  3:990377/1‑149 (MQ=255)
                                                                                                                                     ataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGgt                 >  2:943581/4‑149 (MQ=255)
                                                                                                                                       aaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATc                                                                                        >  4:731953/2‑76 (MQ=255)
                                                                                                                                        aaaaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTa              >  1:530720/1‑149 (MQ=255)
                                                                                                                                        aaaaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTa              <  3:699017/149‑1 (MQ=255)
                                                                                                                                          aaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGc            >  1:1005480/1‑149 (MQ=255)
                                                                                                                                          aaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGc            >  4:937984/1‑149 (MQ=255)
                                                                                                                                              gggCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGTATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGc        <  1:874247/149‑1 (MQ=255)
                                                                                                                                                gCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCgg      >  2:846300/1‑149 (MQ=255)
                                                                                                                                                gCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCgg      <  3:758640/149‑1 (MQ=255)
                                                                                                                                                    gACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTAcc  >  3:179781/1‑149 (MQ=255)
                                                                                                                                                    gACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTAcc  >  2:369525/1‑149 (MQ=255)
                                                                                                                                                    gACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTAcc  <  3:859272/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACC  >  NC_000913/1865534‑1865830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: