Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 169,749 T→A 5.7% intergenic (+22/‑29) fhuA → / → fhuC ferrichrome outer membrane transporter/iron(3+)‑hydroxamate import ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913169,7490TA 5.7% 493.9 / 14.8 141intergenic (+22/‑29)fhuA/fhuCferrichrome outer membrane transporter/iron(3+)‑hydroxamate import ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (67/66);  new base A (4/4);  total (71/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 146 total aligned reads displayed.

TCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGAC  >  NC_000913/169602‑169896
                                                                                                                                                   |                                                                                                                                                   
tCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGAttt                                                                                                                                                    >  3:984381/1‑149 (MQ=255)
tCCAACGTCGCGCTGCCTGTTAACAACATGTACGATCGTGAATACGCCGCCAGCTGCTTTAACACTTATGACTGCTTATGGGGCGCTGAACGCCAGGTCGGTCCAACAGGAACATTACGTTTCTAATTTCTCGTTTGCGGAAAGGAttc                                                                                                                                                    >  2:861361/1‑148 (MQ=255)
    aCGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGt                                                                                                                                                >  3:1019957/1‑149 (MQ=255)
    aCGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGt                                                                                                                                                <  2:784446/149‑1 (MQ=255)
     cGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTg                                                                                                                                               <  2:746644/149‑1 (MQ=255)
      gTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGATTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAGCCTTCCGTTTCTATTTTCTCTTTTGGGGCACGGAAATCCGTGc                                                                                                                                              <  3:145345/149‑1 (MQ=255)
        ggCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGccc                                                                                                                                            <  3:76538/149‑1 (MQ=255)
              gCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGGCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTca                                                                                                                                      <  2:1087911/149‑1 (MQ=255)
                aTGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTcaca                                                                                                                                    >  3:333931/1‑149 (MQ=255)
                 tGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACaa                                                                                                                                   <  3:961054/149‑1 (MQ=255)
                    tAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGtt                                                                                                                                <  3:543329/149‑1 (MQ=255)
                    tAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGtt                                                                                                                                >  3:312682/1‑149 (MQ=255)
                     aacaacCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCAttt                                                                                                                                        >  2:379961/1‑140 (MQ=255)
                     aacaacCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCAttt                                                                                                                                        <  1:379961/140‑1 (MQ=255)
                     aacaacCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTg                                                                                                                               >  4:413028/1‑149 (MQ=255)
                     aacaacCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTGTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTg                                                                                                                               >  3:409604/1‑149 (MQ=255)
                      acaacCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTgg                                                                                                                              <  4:525837/149‑1 (MQ=255)
                       caacCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGc                                                                                                                             <  1:231585/149‑1 (MQ=255)
                        aacCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCt                                                                                                                            <  2:971193/149‑1 (MQ=255)
                        aacCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCt                                                                                                                            <  3:298194/149‑1 (MQ=255)
                          ccTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGt                                                                                                                          >  1:1038788/1‑149 (MQ=255)
                            tGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTa                                                                                                                        >  2:411359/1‑149 (MQ=255)
                               tCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGc                                                                                                                     <  2:423671/149‑1 (MQ=255)
                                 gATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAg                                                                                                                   <  1:865487/149‑1 (MQ=255)
                                      tGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATa                                                                                                              >  2:1087401/1‑149 (MQ=255)
                                      tGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATa                                                                                                              <  1:411359/149‑1 (MQ=255)
                                       gAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATac                                                                                                             <  3:85954/149‑1 (MQ=255)
                                         aTACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATacac                                                                                                           <  4:925645/149‑1 (MQ=255)
                                             gTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAAt                                                                                                       >  3:631647/1‑149 (MQ=255)
                                                 ccAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCAtt                                                                                                   <  2:568534/149‑1 (MQ=255)
                                                  cAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTc                                                                                                  <  2:1038788/149‑1 (MQ=255)
                                                  cAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTc                                                                                                  <  4:312682/149‑1 (MQ=255)
                                                  cAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTc                                                                                                  <  3:651602/149‑1 (MQ=255)
                                                   aGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTcc                                                                                                 >  2:864559/1‑149 (MQ=255)
                                                    gctgctTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCg                                                                                                >  4:295190/1‑149 (MQ=255)
                                                       gctTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATa                                                                                             <  4:640695/149‑1 (MQ=255)
                                                         tttAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATAcc                                                                                           >  1:1002301/1‑149 (MQ=255)
                                                         tttAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATAcc                                                                                           <  1:133267/149‑1 (MQ=255)
                                                           tAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCAc                                                                                         >  1:874789/1‑149 (MQ=255)
                                                            aaCACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACt                                                                                        >  2:363457/1‑149 (MQ=255)
                                                             acacTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACtt                                                                                       <  1:504132/149‑1 (MQ=255)
                                                               acTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCTTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACtttt                                                                                     >  1:646734/1‑149 (MQ=255)
                                                               acTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTc                                                                                                  >  4:441000/1‑136 (MQ=255)
                                                               acTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTc                                                                                                  <  3:441000/136‑1 (MQ=255)
                                                                cTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTg                                                                                    >  2:735151/1‑149 (MQ=255)
                                                                 ttATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGc                                                                                   >  1:955347/1‑149 (MQ=255)
                                                                     ggCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTcc                                                                                                 >  3:688258/1‑131 (MQ=255)
                                                                       ctgctTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCg                                                                             <  1:735151/149‑1 (MQ=255)
                                                                       ctgctTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCg                                                                             >  2:631058/1‑149 (MQ=255)
                                                                       ctgctTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTACCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCg                                                                             <  4:1078730/149‑1 (MQ=255)
                                                                        tgctTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGt                                                                            >  1:524386/1‑149 (MQ=255)
                                                                                gggCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATAtctc                                                                    >  2:53348/1‑149 (MQ=255)
                                                                                gggCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATAtctc                                                                    >  4:205460/1‑149 (MQ=255)
                                                                                gggCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATAtctc                                                                    >  3:506260/1‑149 (MQ=255)
                                                                                    gcAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCttt                                                                <  3:428228/149‑1 (MQ=255)
                                                                                    gcAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCttt                                                                >  2:440755/1‑149 (MQ=255)
                                                                                     cAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTc                                                               <  4:984381/149‑1 (MQ=255)
                                                                                       gAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCgt                                                             <  4:586989/149‑1 (MQ=255)
                                                                                              aGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCg                                                      >  1:1017271/1‑149 (MQ=255)
                                                                                               ggTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCgg                                                     <  1:368219/149‑1 (MQ=255)
                                                                                                gTCGTTGCAACAGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCggg                                                    >  3:612719/1‑149 (MQ=255)
                                                                                                 tCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGAACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCTGGgc                                                   >  4:717244/1‑149 (MQ=255)
                                                                                                     tGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCAc                                               <  2:1017271/149‑1 (MQ=255)
                                                                                                       caaccgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTcc                                                                                                                                                  >  4:55075/1‑48 (MQ=255)
                                                                                                       caaccgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTcc                                                                                                                                                  <  3:55075/48‑1 (MQ=255)
                                                                                                        aaccgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCa                                                                                                                    >  2:738268/1‑77 (MQ=255)
                                                                                                        aaccgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCa                                                                                                                    <  1:738268/77‑1 (MQ=255)
                                                                                                        aaccgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTcc                                                                   >  3:1032625/1‑126 (MQ=255)
                                                                                                        aaccgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCt                                            >  1:254934/1‑149 (MQ=255)
                                                                                                          ccgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCttt                                          >  3:786516/1‑149 (MQ=255)
                                                                                                           cgcaaccTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCtttt                                         <  4:1068941/149‑1 (MQ=255)
                                                                                                                  ttCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCg                                  >  2:175722/1‑149 (MQ=255)
                                                                                                                  ttCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCg                                  >  4:148873/1‑149 (MQ=255)
                                                                                                                  ttCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCg                                  >  1:817607/1‑149 (MQ=255)
                                                                                                                           tAATTTCTGTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCCCACGCTTTTGCATCCGCTGTCGTTa                         >  2:887229/1‑149 (MQ=255)
                                                                                                                             aTTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAAc                       >  1:707841/1‑149 (MQ=255)
                                                                                                                             aTTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAAc                       >  1:739547/1‑149 (MQ=255)
                                                                                                                              tttCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAAcc                      <  1:918898/149‑1 (MQ=255)
                                                                                                                               ttCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCtt                                                                 <  2:505961/105‑1 (MQ=255)
                                                                                                                               ttCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCtt                                                                 >  1:505961/1‑105 (MQ=255)
                                                                                                                                    ttttGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCtttt                                         >  2:364865/1‑124 (MQ=255)
                                                                                                                                    ttttGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCt                >  2:999304/1‑149 (MQ=255)
                                                                                                                                    ttttGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCt                <  2:458762/149‑1 (MQ=255)
                                                                                                                                    ttttGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCt                >  4:343166/1‑149 (MQ=255)
                                                                                                                                    ttttGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCt                >  4:70161/1‑149 (MQ=255)
                                                                                                                                     aaatgggCACGGAAATCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTg               >  4:347511/5‑149 (MQ=255)
                                                                                                                                       atgggCACGGAAATCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTcc                                                                   >  1:149351/3‑95 (MQ=255)
                                                                                                                                       atgggCACGGAAATCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTcc                                                                   <  2:149351/93‑1 (MQ=255)
                                                                                                                                        tgggCACGGAAATCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCtttt                                         <  1:992383/119‑1 (MQ=255)
                                                                                                                                         gggCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCgg           <  2:993693/149‑1 (MQ=255)
                                                                                                                                             aCGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGaaa       >  4:338390/1‑149 (MQ=255)
                                                                                                                                             aCGGATTTCCGTGCCCATTTAACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGaaa       <  2:254934/149‑1 (MQ=255)
                                                                                                                                             aCGGAATGCCGTGCCCATTTCACAAGTAGGCTGTTATGAAGGAATACACGAATCATTCCGATACCACTTGTGCACGGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGGTGTCGTTAACCTTTCCTGCCGGGaaa       <  2:32656/149‑1 (MQ=255)
                                                                                                                                              cGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAg      <  1:488730/149‑1 (MQ=255)
                                                                                                                                               ggATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGt     <  1:546960/149‑1 (MQ=255)
                                                                                                                                               ggATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGt     <  2:49250/149‑1 (MQ=255)
                                                                                                                                               ggATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGt     <  1:82814/149‑1 (MQ=255)
                                                                                                                                                 aTTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGa   <  4:461171/149‑1 (MQ=255)
                                                                                                                                                  tttCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGAc  <  3:227571/149‑1 (MQ=255)
                                                                                                                                                   ttCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCtttt                                         >  4:357403/1‑109 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGTGTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGAC  >  NC_000913/169602‑169896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: