Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,318,095 C→G 100% L594F (TTG→TTC rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,0950CG100.0% 458.0 / NA 124L594F (TTG→TTCrcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (57/67);  total (57/67)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 124 total aligned reads displayed.

ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCAC  >  NC_000913/2317949‑2318235
                                                                                                                                                  |                                                                                                                                            
aCCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                            <  3:291244/149‑1 (MQ=255)
  cTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACa                                                                                                                                          >  4:512693/1‑149 (MQ=255)
   tGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAg                                                                                                                                         <  1:1029499/149‑1 (MQ=255)
    ggAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAgg                                                                                                                                        >  1:1049272/1‑149 (MQ=255)
      aagaagGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGtt                                                                                                                                      <  3:733488/149‑1 (MQ=255)
       agaagGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTg                                                                                                                                     <  4:470868/149‑1 (MQ=255)
         aagGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGga                                                                                                                                   <  3:910112/149‑1 (MQ=255)
         aagGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGga                                                                                                                                   <  2:1080644/149‑1 (MQ=255)
          agGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGgag                                                                                                                                  <  4:669266/149‑1 (MQ=255)
            ggATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGAt                                                                                                                                >  1:97103/1‑149 (MQ=255)
             gATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATg                                                                                                                               <  4:887427/149‑1 (MQ=255)
             gATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATg                                                                                                                               >  4:860491/1‑149 (MQ=255)
             gATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATg                                                                                                                               >  4:602592/1‑149 (MQ=255)
              aTCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGa                                                                                                                              >  4:936532/1‑149 (MQ=255)
               tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAc                                                                                                                             <  4:693268/149‑1 (MQ=255)
                  aaCAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACctg                                                                                                                          >  2:872347/1‑149 (MQ=255)
                  aaCAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACctg                                                                                                                          >  1:197546/1‑149 (MQ=255)
                   aCAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACctgc                                                                                                                         >  3:821235/1‑149 (MQ=255)
                    cAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACctgct                                                                                                                        <  4:815090/149‑1 (MQ=255)
                    cAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGATGGAGATGACctgct                                                                                                                        <  3:968333/149‑1 (MQ=255)
                         gcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACctgct                                                                                                                        <  3:431903/144‑1 (MQ=255)
                         gcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACctgct                                                                                                                        >  4:431903/1‑144 (MQ=255)
                         gcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTaaa                                                                                                                   <  2:197546/149‑1 (MQ=255)
                          caccacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAAc                                                                                                                  <  4:168400/149‑1 (MQ=255)
                            ccacTCCTATCGCCGGTATCCCCACGCCGGTACCGAGAACACGGATAGAGAGATAACCGCCATCCGCGCGAAAATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGACCGGGTTGGAAATGACCTGCTGTAAgcgc                                                                                                                <  4:645455/149‑1 (MQ=255)
                             cacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCa                                                                                                               >  3:451643/1‑149 (MQ=255)
                             cacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACCATACAGCCGGTATCGGTGAATTTTATGGCGCTACTGAACAGGTTGGAGATGACCTGCTGTCAACGCa                                                                                                               >  1:622544/1‑149 (MQ=255)
                              acTTCTTTCGCCGGTATCCCCACGCCGGTATCGTGAACACGGATAGAGAGATAATCGCCATCCACGCGAACATGCAAAACGAAAAAGCCGGTATCGGTGGATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCAt                                                                                                              >  2:788531/1‑149 (MQ=255)
                              acTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCAt                                                                                                              >  2:293860/1‑149 (MQ=255)
                                ttcttTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCg                                                                                                            >  1:348811/1‑149 (MQ=255)
                                  cttTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCggg                                                                                                          >  4:1064163/1‑149 (MQ=255)
                                   tttCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGt                                                                                                         >  4:59318/1‑149 (MQ=255)
                                   tttCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGt                                                                                                         <  1:134532/149‑1 (MQ=255)
                                    ttCGCCGTTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTc                                                                                                        <  3:693541/149‑1 (MQ=255)
                                         cGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCAt                                                                                                   <  1:449352/149‑1 (MQ=255)
                                         cGGTATCCACACGCCGGTATCGCGCACACGGATAGAGAGATAATCGCCATCCGCGCAAACATCCAAAACTATACAGCCGGTATCGGTGAATTCTATGGCGTTACTGAACAGGTTGGAGATGGCCTAGTTTAAACGCATCGGGTCGCTAt                                                                                                   <  4:433291/149‑1 (MQ=255)
                                           gTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCAttt                                                                                                 >  4:548156/1‑149 (MQ=255)
                                           gTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCAttt                                                                                                 >  3:207708/1‑149 (MQ=255)
                                             aTCCCCACGCCGGTATCGCGAACACGGATAGAGATATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGTAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTaa                                                                                               <  1:903659/149‑1 (MQ=255)
                                             aTCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTaa                                                                                               <  2:97103/149‑1 (MQ=255)
                                              tCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAg                                                                                              <  3:272072/149‑1 (MQ=255)
                                                cccACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTc                                                                                                        >  1:802062/1‑137 (MQ=255)
                                                cccACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTc                                                                                                        <  2:802062/137‑1 (MQ=255)
                                                 ccACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGcc                                                                                           >  1:1101403/1‑149 (MQ=255)
                                                  cACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa                                                                                          >  4:587863/1‑149 (MQ=255)
                                                   aCGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCAc                                                                                         >  4:215637/1‑149 (MQ=255)
                                                      cgggTATCGCGAACACGGATAGAGAGATACTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTgg                                                                                      >  3:925988/3‑149 (MQ=255)
                                                        ggTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGca                                                                                    <  2:790677/149‑1 (MQ=255)
                                                             cgcgAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATcc                                                                               <  3:860491/149‑1 (MQ=255)
                                                               cgAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                             >  4:446093/1‑149 (MQ=255)
                                                                 aaCACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGtt                                                                           <  2:981095/149‑1 (MQ=255)
                                                                  acacGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTc                                                                          <  2:622544/149‑1 (MQ=255)
                                                                    acGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCaa                                                                        >  3:144972/1‑149 (MQ=255)
                                                                     cGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAAt                                                                       <  2:876491/149‑1 (MQ=255)
                                                                      ggATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATa                                                                      >  3:862072/1‑149 (MQ=255)
                                                                          agagagATAATCGCGATCCGCTCGAACATGCAAAACTATACAGCCGGTATCGGTGGATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAGACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGc                                                                  >  2:141317/1‑149 (MQ=255)
                                                                           gagagaTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCa                                                                 <  1:524365/149‑1 (MQ=255)
                                                                            agagaTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTc                                                                          <  2:517460/139‑1 (MQ=255)
                                                                            agagaTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTc                                                                          >  1:517460/1‑139 (MQ=255)
                                                                             gagaTAATCGCCATCCGTGCGAACAGGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGt                                                               >  2:599919/1‑149 (MQ=255)
                                                                              agaTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTa                                                              <  1:293860/149‑1 (MQ=255)
                                                                                 tAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACaa                                                           <  3:557423/149‑1 (MQ=255)
                                                                                  aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACctgct                                                                                                                        <  2:997753/87‑1 (MQ=255)
                                                                                  aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACctgct                                                                                                                        >  1:997753/1‑87 (MQ=255)
                                                                                   aTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGc                                                         <  1:772708/149‑1 (MQ=255)
                                                                                   aTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGc                                                         >  4:1049183/1‑149 (MQ=255)
                                                                                     cGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCt                                                       <  3:1064163/149‑1 (MQ=255)
                                                                                                cgAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCg                                            <  2:181793/149‑1 (MQ=255)
                                                                                                 gAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGt                                           <  1:872347/149‑1 (MQ=255)
                                                                                                 gAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGCAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGt                                           >  3:678907/1‑149 (MQ=255)
                                                                                                   aCATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACaa                                                           <  2:867957/131‑1 (MQ=255)
                                                                                                   aCATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTac                                         <  3:548156/149‑1 (MQ=255)
                                                                                                      tGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac                                      >  1:1020053/1‑149 (MQ=255)
                                                                                                        cAAAACTATACAGCCGGTATCGATGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGGGTAccacca                                    <  1:948158/149‑1 (MQ=255)
                                                                                                         aaaaCTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAg                                   >  2:881548/1‑149 (MQ=255)
                                                                                                         aaaaCTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAg                                   >  4:372835/1‑149 (MQ=255)
                                                                                                         aaaaCTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAg                                   >  4:47892/1‑149 (MQ=255)
                                                                                                          aaaCTATACAGCCGGTCTCGGTGAATTTTATGGCGTTACTGAAAAGGTTGGAGATGACCTGCTGTAAACGCATAGGGTGGCCATTTAAGGTCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGc                                  >  4:882349/1‑149 (MQ=255)
                                                                                                              tataCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTa                              <  4:207708/149‑1 (MQ=255)
                                                                                                              tataCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTa                              >  2:141353/1‑149 (MQ=255)
                                                                                                               ataCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTaa                             <  2:1020053/149‑1 (MQ=255)
                                                                                                                  cAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATa                          <  4:302794/149‑1 (MQ=255)
                                                                                                                   aGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAg                         >  3:9600/1‑149 (MQ=255)
                                                                                                                     ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGtt                       <  3:47892/149‑1 (MQ=255)
                                                                                                                     ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGtt                       <  2:11997/149‑1 (MQ=255)
                                                                                                                     ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGtt                       >  2:587262/1‑149 (MQ=255)
                                                                                                                     ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGGCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGtt                       <  4:678907/149‑1 (MQ=255)
                                                                                                                           tCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATGAAGCAGTACAAGCCTGACTGCTTGCGTACCACCAGCGGTAAATa                          <  1:843794/140‑1 (MQ=255)
                                                                                                                              gTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAt              >  2:264297/1‑149 (MQ=255)
                                                                                                                              gTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAt              <  1:264297/149‑1 (MQ=255)
                                                                                                                                  gttttATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCTGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTgg          <  1:788531/148‑1 (MQ=255)
                                                                                                                                  aTTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTgg          <  2:1101403/149‑1 (MQ=255)
                                                                                                                                   ttttATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGACGTGGt         >  4:400843/1‑149 (MQ=255)
                                                                                                                                       aTGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTtcat     <  2:727893/149‑1 (MQ=255)
                                                                                                                                       aTGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTtcat     <  1:671729/149‑1 (MQ=255)
                                                                                                                                        tGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTtcatc    <  2:1049272/149‑1 (MQ=255)
                                                                                                                                          gCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAt              <  1:542836/137‑1 (MQ=255)
                                                                                                                                          gCGTTACTGAACAGGTTGGAGAGGACCTGCTTTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCGATAAACCAGACAAAGCCTAACTGCTTGCGTACCACCAGCGGAAAATAGTTGGCGGTGATGTGGTCCATCAc  >  1:655313/1‑149 (MQ=255)
                                                                                                                                           cGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAg                                                                                              >  2:594374/1‑56 (MQ=255)
                                                                                                                                           cGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAg                                                                                              <  1:594374/56‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCAC  >  NC_000913/2317949‑2318235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: