Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,428,699 A→T 5.0% intergenic (‑73/+22) ubiX ← / ← purF 3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase/amidophosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,428,6990AT 5.0% 413.9 / 9.1 119intergenic (‑73/+22)ubiX/purF3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase/amidophosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (53/60);  new base T (3/3);  total (56/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

Only 100 of 119 total aligned reads displayed.

AGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTA  >  NC_000913/2428556‑2428843
                                                                                                                                               |                                                                                                                                                
aGAACCTGTAATAAGCGCACGCCATAAATAGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGc                                                                                                                                             >  3:925904/1‑149 (MQ=255)
    ccTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCAccc                                                                                                                                         >  3:716823/1‑149 (MQ=255)
     cTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCt                                                                                                                                        >  3:262729/1‑149 (MQ=255)
      tGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTc                                                                                                                                       >  2:482924/1‑149 (MQ=255)
           taaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGa                                                                                                                                  >  1:546582/1‑149 (MQ=255)
           taaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGa                                                                                                                                  >  2:928895/1‑149 (MQ=255)
            aaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGaa                                                                                                                                 <  2:836993/149‑1 (MQ=255)
            aaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGaa                                                                                                                                 <  3:291797/149‑1 (MQ=255)
            aaGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGaa                                                                                                                                 <  1:26652/149‑1 (MQ=255)
             aGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAg                                                                                                                                <  2:271109/149‑1 (MQ=255)
             aGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAg                                                                                                                                <  4:817347/149‑1 (MQ=255)
                    gCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGcatcat                                                                                                                         >  4:53946/1‑149 (MQ=255)
                      cATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATcc                                                                                                                       >  2:758811/1‑149 (MQ=255)
                      cATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATcc                                                                                                                       >  2:522781/1‑149 (MQ=255)
                        tAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCtt                                                                                                                     <  2:510305/149‑1 (MQ=255)
                            tCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGtt                                                                                                                 >  1:364695/1‑149 (MQ=255)
                             cgcgCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTa                                                                                                                <  1:928895/149‑1 (MQ=255)
                                  cgcTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCAt                                                                                                           >  3:872393/1‑149 (MQ=255)
                                  cgcTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCAt                                                                                                           >  2:561588/1‑149 (MQ=255)
                                         aCCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCgaga                                                                                                    >  4:476426/1‑149 (MQ=255)
                                         aCCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTATCAGACTTGTAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCAGCATCCTTCGTTATGCATTTCgaga                                                                                                    <  1:782366/149‑1 (MQ=255)
                                           cGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGAtt                                                                                                  <  1:218051/149‑1 (MQ=255)
                                              tGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCATCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTc                                                                                               <  4:657312/149‑1 (MQ=255)
                                              tGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTc                                                                                                                    <  4:466222/128‑1 (MQ=255)
                                              tGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTc                                                                                                                    >  3:466222/1‑128 (MQ=255)
                                              tGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTc                                                                                               <  1:318939/149‑1 (MQ=255)
                                                    cTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTc                                                                                         >  3:958710/1‑149 (MQ=255)
                                                    cTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTc                                                                                         >  3:372818/1‑149 (MQ=255)
                                                     tACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCg                                                                                        >  2:263456/1‑149 (MQ=255)
                                                       cAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGtt                                                                                      <  4:115631/149‑1 (MQ=255)
                                                       cAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGtt                                                                                      <  4:833994/149‑1 (MQ=255)
                                                        aaTGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTc                                                                                     <  1:988880/149‑1 (MQ=255)
                                                         aTGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCt                                                                                    <  2:81672/149‑1 (MQ=255)
                                                           gAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGa                                                                                  <  4:61777/149‑1 (MQ=255)
                                                              tCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGt                                                                               >  1:203156/1‑149 (MQ=255)
                                                                 tttCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGc                                                                            >  3:151879/1‑149 (MQ=255)
                                                                  ttCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCa                                                                           <  3:463309/149‑1 (MQ=255)
                                                                      tAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGc                                                                       >  1:92466/1‑149 (MQ=255)
                                                                      tAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGc                                                                       >  2:778579/1‑149 (MQ=255)
                                                                      tAAATAGTTGCCCTGCCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCTGCACCCTCAGCGAAGGCATAATCCTTCGTTATGCATTTCGAGCTTTTCCACTTCGTTCTGACGTTGCACTGc                                                                       >  2:466591/1‑149 (MQ=255)
                                                                         aTAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTc                                                                                                        >  4:907912/1‑113 (MQ=255)
                                                                         aTAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTc                                                                                                        <  3:907912/113‑1 (MQ=255)
                                                                          tAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTg                                                                   <  1:186977/149‑1 (MQ=255)
                                                                              tGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGt                                                               <  1:564468/149‑1 (MQ=255)
                                                                               gCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGtc                                                              <  1:845415/149‑1 (MQ=255)
                                                                               gCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGACTTTCCACTTCGTTCTGACGTTGCAATGCTTTGGCGtc                                                              <  1:527271/149‑1 (MQ=255)
                                                                                cccTGTCAGACTTGCAGGCAGACTCTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGtca                                                             >  2:610701/1‑149 (MQ=255)
                                                                                 ccTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGtcat                                                            >  2:801504/1‑149 (MQ=255)
                                                                                 ccTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGtcat                                                            <  3:782223/149‑1 (MQ=255)
                                                                                 ccTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGtcat                                                            <  3:787181/149‑1 (MQ=255)
                                                                                   tGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGtcatca                                                          <  1:212755/149‑1 (MQ=255)
                                                                                     tCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCAtt                                                        >  4:757874/1‑149 (MQ=255)
                                                                                     tCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCAtt                                                        >  1:1077919/1‑149 (MQ=255)
                                                                                     tCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGATGCAAGTCAGGGTGCCGGACCGGCACCCTCAGCGAAAGGATCATCCGTCGTTATGCATTTCGAGATTATCCACTTCGATCTGACGTTGCACTGCATTGGCGTCATCAtt                                                        >  3:667913/1‑149 (MQ=255)
                                                                                       aGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTac                                                      >  4:1056785/1‑149 (MQ=255)
                                                                                       aGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTac                                                      >  2:212837/1‑149 (MQ=255)
                                                                                       aGACTTGCAGGCAGACTTTGCAGGATTTCGACGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTac                                                      <  3:22382/149‑1 (MQ=255)
                                                                                         aCTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTAGCGTCATCATTacgt                                                    >  3:337032/1‑149 (MQ=255)
                                                                                           ttGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGTTGCCAGACCGGCACCCTCAGCGAAGGTATCATCCTTCGTTATTCATTTCGAGATTTTCCGCTTCGTTCTGAAGTTGCACTTCTTTGGCGTCATCATTacgtaa                                                  <  2:86342/149‑1 (MQ=255)
                                                                                           ttGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacgtaa                                                  >  4:962881/1‑149 (MQ=255)
                                                                                           ttGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacgtaa                                                  <  4:651398/149‑1 (MQ=255)
                                                                                            tGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTTCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacgtaac                                                 <  4:522452/149‑1 (MQ=255)
                                                                                            tGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacgtaac                                                 <  4:919277/149‑1 (MQ=255)
                                                                                                ggcagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTAt                                             <  2:991123/149‑1 (MQ=255)
                                                                                                 gcagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATc                                            <  3:294023/149‑1 (MQ=255)
                                                                                                 gcagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATc                                            <  1:1063923/149‑1 (MQ=255)
                                                                                                 gcagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATc                                            <  1:758811/149‑1 (MQ=255)
                                                                                                 gcagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATc                                            >  4:37421/1‑149 (MQ=255)
                                                                                                 gcagACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATc                                            >  4:366674/1‑149 (MQ=255)
                                                                                                   agACTTTGCAGGATTTCGGCGGGTGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGTCATCATCCTTCGTTATGCATTTCGAGCTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTAGGTAACGTATCGa                                          >  2:163698/1‑149 (MQ=255)
                                                                                                   agACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGa                                          >  2:854660/1‑149 (MQ=255)
                                                                                                    gACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTacgta                                                   >  1:563763/1‑139 (MQ=255)
                                                                                                      cTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGa                                       <  1:94339/149‑1 (MQ=255)
                                                                                                       tttGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCttt                                                                    <  3:634914/119‑1 (MQ=255)
                                                                                                       tttGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCttt                                                                    >  4:634914/1‑119 (MQ=255)
                                                                                                       tttGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGaa                                      >  1:736498/1‑149 (MQ=255)
                                                                                                       tttGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGaa                                      <  4:19904/149‑1 (MQ=255)
                                                                                                            aGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGa                                 <  1:7559/149‑1 (MQ=255)
                                                                                                             ggATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCttt                                                                    >  4:786772/1‑113 (MQ=255)
                                                                                                             ggATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCttt                                                                    <  3:786772/113‑1 (MQ=255)
                                                                                                             ggATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCATTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAg                                >  3:505625/1‑149 (MQ=255)
                                                                                                                        ggAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGAt                     <  4:151879/149‑1 (MQ=255)
                                                                                                                         gAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc                    <  1:482924/149‑1 (MQ=255)
                                                                                                                              gCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACAt               <  4:856929/149‑1 (MQ=255)
                                                                                                                                  gTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCttt           <  4:947931/149‑1 (MQ=255)
                                                                                                                                  gTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCttt           <  4:166889/149‑1 (MQ=255)
                                                                                                                                  gTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCttt           <  3:596718/149‑1 (MQ=255)
                                                                                                                                   tgaGGGTGCCGGTCTGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGtt                                                                                      <  3:794016/71‑1 (MQ=37)
                                                                                                                                   tgaGGGTGCCGGTCTGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGtt                                                                                      >  4:794016/3‑73 (MQ=38)
                                                                                                                                   tgaGGGTGCCGGTCTGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTg          <  1:323607/147‑1 (MQ=255)
                                                                                                                                   tCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTg          <  1:610701/149‑1 (MQ=255)
                                                                                                                                    gaGGGTGCCGGTCTGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTgg         >  2:1035727/2‑149 (MQ=255)
                                                                                                                                    cAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTgg         <  2:574339/149‑1 (MQ=255)
                                                                                                                                     aGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt        <  3:757874/149‑1 (MQ=255)
                                                                                                                                     aGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt        >  1:407072/1‑149 (MQ=255)
                                                                                                                                      gggTGCCAGACCGGCACCCTCAGCGAAGGCATAATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTg       <  2:1010159/149‑1 (MQ=255)
                                                                                                                                          gCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGt   >  3:459762/1‑149 (MQ=255)
                                                                                                                                           ccAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa  <  3:53946/149‑1 (MQ=255)
                                                                                                                                           ccAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa  <  3:962881/149‑1 (MQ=255)
                                                                                                                                           ccAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTACGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa  >  4:507111/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                
AGAACCTGTAATAAGCGCACGCCATAAATCGCGCCGCTGGCACCGCTGATGCCTACAATGAGTCGTTTCATAAATAGTTGCCCTGTCAGACTTGCAGGCAGACTTTGCAGGATTTCGGCGGGAGTTGCAAGTCAGGGTGCCAGACCGGCACCCTCAGCGAAGGCATCATCCTTCGTTATGCATTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTA  >  NC_000913/2428556‑2428843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: