Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,497,023 A→T 5.0% intergenic (+31/+34) ypdK → / ← alaC inner membrane protein/glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,497,0230AT 5.0% 413.1 / 9.4 119intergenic (+31/+34)ypdK/alaCinner membrane protein/glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase
Reads supporting (aligned to +/- strand):  ref base A (52/61);  new base T (2/4);  total (54/65)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 123 total aligned reads displayed.

AATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGT  >  NC_000913/2496875‑2497171
                                                                                                                                                    |                                                                                                                                                    
aaTGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTGCTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCa                                                                                                                                                      >  3:760478/1‑149 (MQ=255)
 aTGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCAt                                                                                                                                                     >  3:1010855/1‑149 (MQ=255)
 aTGAAGTCATTCAGACGTAACAGTAATTACGCGGAGAGATTTAAAGTGAAATCTTTCTTTATGAGCATTTCTTTTATGGACATCGTTTGCGCCGGTACTTTTGCCCTGATGATCTAAACGGAAACTGTGCAATGCAAAACAGGCGCCAt                                                                                                                                                     >  2:819052/1‑149 (MQ=255)
  tGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATc                                                                                                                                                    <  1:823167/149‑1 (MQ=255)
    aaGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAg                                                                                                                                                  >  4:775454/1‑149 (MQ=255)
     aGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAgg                                                                                                                                                 >  4:194202/1‑149 (MQ=255)
      gTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGc                                                                                                                                                >  2:997697/1‑149 (MQ=255)
        cATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTc                                                                                                                                              <  1:597643/149‑1 (MQ=255)
         aTTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTcc                                                                                                                                             <  1:997697/149‑1 (MQ=255)
          ttCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCt                                                                                                                                            <  1:834733/149‑1 (MQ=255)
          ttCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCt                                                                                                                                            >  2:145635/1‑149 (MQ=255)
          ttCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCt                                                                                                                                            >  2:228683/1‑149 (MQ=255)
             aGGCGTAACAGTAAATACGCGGAGAGATGTATAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCAACGTTTGGGCCGGTACCTTTGCCCTGATGATCTAACAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGtt                                                                                                                                         <  3:903056/149‑1 (MQ=255)
              ggCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCTGATGGCTCCTGttt                                                                                                                                        <  2:268522/149‑1 (MQ=255)
              ggCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGttt                                                                                                                                        <  4:734575/149‑1 (MQ=255)
                 gTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCTGATGGCTCCTGtttttc                                                                                                                                     <  1:993581/149‑4 (MQ=255)
                    aCAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCAttt                                                                                                                                  >  1:411964/1‑149 (MQ=255)
                      aGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCt                                                                                                                                <  3:981654/149‑1 (MQ=255)
                      aGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCt                                                                                                                                <  1:546246/149‑1 (MQ=255)
                        tAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTga                                                                                                                              <  3:194202/149‑1 (MQ=255)
                               gcgGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGgt                                                                                                                       <  2:270847/149‑1 (MQ=255)
                               gcgGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGgt                                                                                                                       <  2:829753/149‑1 (MQ=255)
                                 ggAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGgtgt                                                                                                                     <  3:638899/149‑1 (MQ=255)
                                 ggAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGgtgt                                                                                                                     >  1:526042/1‑149 (MQ=255)
                                 ggAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTGTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTTCTGTTTGCATTTCTGACGACGgtgt                                                                                                                     <  2:856118/149‑1 (MQ=255)
                                     agaTGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTAt                                                                                                                 >  1:417542/1‑149 (MQ=255)
                                       aTGTAAAGTGAAATATTTCTTTGTGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGGCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTc                                                                                                               >  3:59136/1‑149 (MQ=255)
                                         gTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCcg                                                                                                             >  3:25250/1‑149 (MQ=255)
                                         gTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCcg                                                                                                             >  1:1077147/1‑149 (MQ=255)
                                          tAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCcgc                                                                                                            <  1:932081/149‑1 (MQ=255)
                                              gTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGttt                                                                                                        <  3:392575/149‑1 (MQ=255)
                                               tGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCAt                                                                                                                                                     <  2:97595/103‑1 (MQ=255)
                                                gAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTc                                                                                                      <  4:153719/149‑1 (MQ=255)
                                                  aaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGt                                                                                                    <  1:613318/149‑1 (MQ=255)
                                                  aaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGt                                                                                                    <  3:775454/149‑1 (MQ=255)
                                                     atTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGaa                                                                                                 <  2:848427/149‑1 (MQ=255)
                                                     atTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGaa                                                                                                 <  1:415081/149‑1 (MQ=255)
                                                          tttATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGt                                                                                            >  3:172145/1‑149 (MQ=255)
                                                            tATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGttt                                                                                          <  1:228683/149‑1 (MQ=255)
                                                              tGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTtgc                                                                                        <  1:145635/149‑1 (MQ=255)
                                                               gggCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTcc                                                                                                              <  4:380913/126‑1 (MQ=255)
                                                               gggCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTcc                                                                                                              >  3:380913/1‑126 (MQ=255)
                                                                ggCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTtgctg                                                                                      >  1:1033746/1‑149 (MQ=255)
                                                                    tttCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGttt                                                                                          >  4:671264/1‑141 (MQ=255)
                                                                    tttCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGttt                                                                                          <  3:671264/141‑1 (MQ=255)
                                                                     ttCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGc                                                                                 <  2:411964/149‑1 (MQ=255)
                                                                     ttCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGc                                                                                 <  2:589629/149‑1 (MQ=255)
                                                                     ttCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGc                                                                                 >  4:565519/1‑149 (MQ=255)
                                                                        ttttATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCggg                                                                              >  3:394968/1‑149 (MQ=255)
                                                                               gTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCGATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTaaa                                                                       <  2:671832/149‑1 (MQ=255)
                                                                               gTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGAGGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTaaa                                                                       >  1:934226/1‑149 (MQ=255)
                                                                                 cATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAAcc                                                                     <  1:101859/149‑1 (MQ=255)
                                                                                  aTCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGttt                                                                                          <  3:1046423/127‑1 (MQ=255)
                                                                                  aTCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCg                                                                    >  4:713812/1‑149 (MQ=255)
                                                                                   tCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCTGATGGCTCCTGtt                                                                                                                                         >  2:799144/1‑79 (MQ=38)
                                                                                   tCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCTGATGGCTCCTGtt                                                                                                                                         <  1:799144/79‑1 (MQ=38)
                                                                                    cGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTc                                                                  <  2:802503/149‑1 (MQ=255)
                                                                                         gggCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGGCGACGGTGTTTATTACGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCAc                                                             >  2:909073/1‑149 (MQ=255)
                                                                                             cGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGTACGGaa                                                         >  1:539324/1‑149 (MQ=255)
                                                                                             cGGTACCTTTGCCCTGATGATCTAAAAGCAAACTGTACAACGAAAAACGGGTGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCGGCGTTTTCGTGAGTATGTTTGCTGCTAACGGGCAGTAAACCGTCGGCACCGaa                                                         <  2:143028/149‑1 (MQ=255)
                                                                                                   ttttGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGc                                                   >  4:44577/1‑149 (MQ=255)
                                                                                                   ttttGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGc                                                   >  1:435124/1‑149 (MQ=255)
                                                                                                   ttttGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGc                                                   >  4:809348/1‑149 (MQ=255)
                                                                                                    tttGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCt                                                  <  4:372634/149‑1 (MQ=255)
                                                                                                     ttGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTTAATATGTTTGCTGCTGGCGGGTAGTAATCCGACGGCACGGAACATCGCtt                                                 <  4:1076199/149‑1 (MQ=255)
                                                                                                        cccTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTaa                                              <  2:1033746/149‑1 (MQ=255)
                                                                                                           tgatgaTCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATcc                                           >  1:974816/1‑149 (MQ=255)
                                                                                                            gatgatCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATccc                                          >  1:812261/1‑149 (MQ=255)
                                                                                                             atgatCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCct                                         <  1:560821/149‑1 (MQ=255)
                                                                                                               gatCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCctct                                       >  1:544804/1‑149 (MQ=255)
                                                                                                               gatCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCctct                                       <  1:196441/149‑1 (MQ=255)
                                                                                                                 tCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTgac                                                                                                                             <  3:367384/61‑1 (MQ=255)
                                                                                                                 tCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTgac                                                                                                                             >  4:367384/1‑61 (MQ=255)
                                                                                                                 tCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGa                                     <  2:208349/149‑1 (MQ=255)
                                                                                                                  cTAAAAGCAACCTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGAGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCGCTGAt                                    <  2:812411/149‑1 (MQ=255)
                                                                                                                   tAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATg                                   >  3:828117/1‑149 (MQ=255)
                                                                                                                   tAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATg                                   >  3:862422/1‑149 (MQ=255)
                                                                                                                   tAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATg                                   >  4:1075547/1‑149 (MQ=255)
                                                                                                                   tAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATg                                   >  2:997374/1‑149 (MQ=255)
                                                                                                                      aaGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGcc                                >  2:26020/1‑149 (MQ=255)
                                                                                                                       aGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCt                               <  1:16229/149‑1 (MQ=255)
                                                                                                                                tgCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATAc                      <  3:809348/149‑1 (MQ=255)
                                                                                                                                tgCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATAc                      <  3:44577/149‑1 (MQ=255)
                                                                                                                                   aaTGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGgta                                                                            >  4:834871/1‑92 (MQ=255)
                                                                                                                                   aaTGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCctc                                        <  3:805152/128‑1 (MQ=255)
                                                                                                                                   aaTGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCctc                                        >  4:805152/1‑128 (MQ=255)
                                                                                                                                    aTGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTc                  <  1:1051063/149‑1 (MQ=255)
                                                                                                                                      gAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGGCGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTcgc                <  1:909073/149‑1 (MQ=255)
                                                                                                                                         aaaCAGGAGCCTGATGGCTCCTGTTTGCATTTCTGACGACGGTTTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTc                  <  1:78290/144‑1 (MQ=255)
                                                                                                                                         aaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAAt                        >  2:556898/1‑138 (MQ=255)
                                                                                                                                         aaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAAt                        <  1:556898/138‑1 (MQ=255)
                                                                                                                                         aaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGt             >  4:1021721/1‑149 (MQ=255)
                                                                                                                                          aaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGtt            <  2:726146/149‑1 (MQ=255)
                                                                                                                                           aCAGGAGCCATCAGGCTCCTGTTTGCTTTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGttt           <  2:417542/149‑1 (MQ=255)
                                                                                                                                              ggAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCa        >  3:659971/1‑149 (MQ=255)
                                                                                                                                                aGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAAt      <  2:1043534/149‑1 (MQ=255)
                                                                                                                                                 gCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATc     <  4:683159/149‑1 (MQ=255)
                                                                                                                                                    aTCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGt  <  1:430667/149‑1 (MQ=255)
                                                                                                                                                    aTCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGt  >  1:152539/1‑149 (MQ=255)
                                                                                                                                                    aTCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGt  <  3:418669/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGT  >  NC_000913/2496875‑2497171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: