Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,656,391 G→A 5.3% intergenic (‑33/+145) pepB ← / ← iscX aminopeptidase B/Fe(2+) donor and activity modulator for cysteine desulfurase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,656,3910GA 5.3% 373.2 / 6.5 113intergenic (‑33/+145)pepB/iscXaminopeptidase B/Fe(2+) donor and activity modulator for cysteine desulfurase
Reads supporting (aligned to +/- strand):  ref base G (61/46);  new base A (4/2);  total (65/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 115 total aligned reads displayed.

TCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTAT  >  NC_000913/2656243‑2656539
                                                                                                                                                    |                                                                                                                                                    
tCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAg                                                                                                                                                      >  1:10207/1‑149 (MQ=255)
  tGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGtt                                                                                                                                                    <  3:360561/149‑1 (MQ=255)
   gCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTg                                                                                                                                                   <  3:954306/149‑1 (MQ=255)
     cccGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGc                                                                                                                                                 >  1:1029783/1‑149 (MQ=255)
     cccGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGc                                                                                                                                                 >  1:911506/1‑149 (MQ=255)
      ccGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCa                                                                                                                                                >  4:794020/1‑149 (MQ=255)
       cGTTCAAATGCAGGGTAATGCCGTCAGTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAg                                                                                                                                               >  3:906971/1‑149 (MQ=255)
        gTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGc                                                                                                                                              >  2:1024073/1‑149 (MQ=255)
        gTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGc                                                                                                                                              <  1:702848/149‑1 (MQ=255)
            aaaTGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCa                                                                                                                                          <  3:620661/149‑1 (MQ=255)
             aaTGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCTGCCAACTGGCGGcccttt                                                                                                                                         <  2:379785/149‑4 (MQ=255)
               tGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGa                                                                                                                                       <  4:38516/149‑1 (MQ=255)
                gCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGAt                                                                                                                                      >  1:551100/1‑149 (MQ=255)
                      tAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGTGTAGAGCGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTcc                                                                                                                                <  2:919736/149‑1 (MQ=255)
                         tGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCt                                                                                                                             >  1:446104/1‑149 (MQ=255)
                         tGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCAATTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCt                                                                                                                             >  4:928034/1‑149 (MQ=255)
                             gTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAAt                                                                                                                         >  3:243492/1‑149 (MQ=255)
                             gTCATTATTAATGCTGTAAGTTGCTTTTTATCCCCAGCGCGCGTCGGCAGGTTTGGTGGAGAGGGTAAGCTTCATCGATTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCGCCAGGATTTTTCCGCTAAAt                                                                                                                         >  2:745369/1‑149 (MQ=255)
                                attattAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGAt                                                                                                                      >  1:770232/1‑149 (MQ=255)
                                   attaATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTcg                                                                                                                   >  3:1076694/1‑149 (MQ=255)
                                   attaATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATACTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTcg                                                                                                                   >  3:1024074/1‑149 (MQ=255)
                                    ttaATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTcc                                                                                                                                >  1:553086/1‑135 (MQ=255)
                                    ttaATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTcc                                                                                                                                <  2:553086/135‑1 (MQ=255)
                                      aacgCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAATGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGt                                                                                                                >  4:354809/4‑149 (MQ=255)
                                       aTGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGtt                                                                                                               >  1:1086622/1‑149 (MQ=255)
                                          cTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCa                                                                                                            <  3:59766/149‑1 (MQ=255)
                                          cTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCa                                                                                                            <  3:346255/149‑1 (MQ=255)
                                           tGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAg                                                                                                           <  4:222741/149‑1 (MQ=255)
                                           tGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAg                                                                                                           >  2:382377/1‑149 (MQ=255)
                                              aaGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGaa                                                                                                        <  2:642246/149‑1 (MQ=255)
                                                 ttGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCCGGAAggc                                                                                                     >  2:759706/1‑149 (MQ=255)
                                                 ttGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAggc                                                                                                     >  3:740142/1‑149 (MQ=255)
                                                 ttGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAggc                                                                                                     <  3:67358/149‑1 (MQ=255)
                                                 ttGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAggc                                                                                                     >  1:250457/1‑149 (MQ=255)
                                                 ttGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAggc                                                                                                     >  2:54125/1‑149 (MQ=255)
                                                 ttGCTTTTACTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAggc                                                                                                     >  3:996624/1‑149 (MQ=255)
                                                  tGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAggcg                                                                                                    >  3:510387/1‑149 (MQ=255)
                                                   gCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAggcgg                                                                                                   >  2:404969/1‑149 (MQ=255)
                                                    cTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAggcggc                                                                                                  >  1:427805/1‑149 (MQ=255)
                                                     tttttCTCCCCAGCGCGCGTCGGCAGGTTGGGTTGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCa                                                                                                 >  3:879331/1‑149 (MQ=255)
                                                        ttCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAagt                                                                                              <  1:470699/149‑1 (MQ=255)
                                                           tcCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAagtgag                                                                                           <  1:404969/149‑1 (MQ=255)
                                                            ccccAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAagtgagt                                                                                          <  1:382377/149‑1 (MQ=255)
                                                            ccccAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAagtgagtg                                                                                         >  2:78652/1‑149 (MQ=255)
                                                             cccAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAagtgagtg                                                                                         <  3:1036355/149‑1 (MQ=255)
                                                             cccAGCGCGCGTCGGCAGGTTGAGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAATGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGt                                                                                                                >  4:1014131/1‑126 (MQ=255)
                                                             cccAGCGCGCGTCGGCAGGTTGAGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGt                                                                                                                <  3:1014131/126‑1 (MQ=255)
                                                              ccggcgcgcGTCGGCAGGTTGGGTAGAGAGGGTACTCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAgagagcga                                                                                        >  3:719462/4‑149 (MQ=255)
                                                              ccAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTgg                                                                                                                                                  >  2:975760/1‑91 (MQ=255)
                                                              ccAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTgg                                                                                                                                                  <  1:975760/91‑1 (MQ=255)
                                                              ccAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAgtgagtga                                                                                        <  2:427805/149‑1 (MQ=255)
                                                                   gcgcgTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGccc                                                                                   <  3:887635/149‑1 (MQ=255)
                                                                            cAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCAt                                                                          <  4:412175/149‑1 (MQ=255)
                                                                             aGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATa                                                                         <  3:794020/149‑1 (MQ=255)
                                                                                ttGGGTTGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGAt                                                                      <  4:879331/149‑1 (MQ=255)
                                                                                ttGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGAt                                                                      >  1:172491/1‑149 (MQ=255)
                                                                                    gTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTcgc                                                                                                                  >  4:291363/1‑101 (MQ=255)
                                                                                    gTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTcgc                                                                                                                  <  3:291363/101‑1 (MQ=255)
                                                                                    gTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAagt                                                                                              <  3:84593/121‑1 (MQ=255)
                                                                                      agagagGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTAt                                                                <  3:463542/149‑1 (MQ=255)
                                                                                       gagagGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTAtg                                                               >  1:376378/1‑149 (MQ=255)
                                                                                          agGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGa                                                            <  4:996624/149‑1 (MQ=255)
                                                                                          agGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGa                                                            <  2:317942/149‑1 (MQ=255)
                                                                                           gggTAAGCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGCCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCATGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGAc                                                           <  2:437590/149‑1 (MQ=255)
                                                                                            ggTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACt                                                          >  3:555721/1‑149 (MQ=255)
                                                                                             gTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTg                                                         >  2:836806/1‑149 (MQ=255)
                                                                                              tAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTgg                                                        <  2:782607/149‑1 (MQ=255)
                                                                                                    tCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGaa                                                  >  2:896092/1‑149 (MQ=255)
                                                                                                      aTCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAACCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACg                                                >  4:870945/1‑149 (MQ=255)
                                                                                                       tCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGa                                               <  4:969522/149‑1 (MQ=255)
                                                                                                       tCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGa                                               <  1:38484/149‑1 (MQ=255)
                                                                                                         gCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAgc                                             >  4:408409/1‑149 (MQ=255)
                                                                                                             cTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAg                                         <  2:172491/149‑1 (MQ=255)
                                                                                                               gTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCa                                                                                                                                                <  1:770652/44‑1 (MQ=255)
                                                                                                                tCATTTTAGTTATCGTGCTTGGTAAAACGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTCACTGATTCGCGTTGCACGAAGGCGGTAAGTGAGTGACGCCCCAGAAGAATAGGTAACTATGTGACGTGGGTGAACGAGCGCAGCCa                                      >  2:790648/1‑149 (MQ=255)
                                                                                                                   ttttAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACg                                   >  4:727136/1‑149 (MQ=255)
                                                                                                                   ttttAGTTATCCTGCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACg                                   <  1:280035/149‑1 (MQ=255)
                                                                                                                          tATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTgc                            <  4:510387/149‑1 (MQ=255)
                                                                                                                           aTCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTgcg                           >  1:966501/1‑149 (MQ=255)
                                                                                                                               ttcttTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGc                                                                                     <  4:397212/87‑1 (MQ=255)
                                                                                                                               ttcttTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGc                                                                                     >  3:397212/1‑87 (MQ=255)
                                                                                                                               ttcttTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCAt                                                                          <  3:907154/98‑1 (MQ=255)
                                                                                                                               ttcttTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCAt                                                                          >  4:907154/1‑98 (MQ=255)
                                                                                                                               ttcttTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGt                       >  2:978920/1‑149 (MQ=255)
                                                                                                                               ttcttTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGt                       >  4:249908/1‑149 (MQ=255)
                                                                                                                                tcttTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTg                      >  3:849932/1‑149 (MQ=255)
                                                                                                                                  tttGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGaa                    <  2:10207/149‑1 (MQ=255)
                                                                                                                                     gTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCa                 <  1:464582/149‑1 (MQ=255)
                                                                                                                                      tAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCAt                <  1:15171/149‑1 (MQ=255)
                                                                                                                                        ctggggCTGCCAACTGGCGGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCTTGa              >  3:436674/4‑149 (MQ=37)
                                                                                                                                        aaaGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGa              >  4:126679/1‑149 (MQ=255)
                                                                                                                                        aaaGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGa              >  3:196207/1‑149 (MQ=255)
                                                                                                                                         tggggCTGCCAACTGGCGGCCCCAGGATTTTTCCGCTAAGTGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTAt                                                                <  4:183891/96‑1 (MQ=255)
                                                                                                                                         aaGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGCGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGAc             >  1:356221/1‑149 (MQ=255)
                                                                                                                                           gggCTGCCAACTGGCGGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGAcgc           >  3:368385/1‑149 (MQ=255)
                                                                                                                                           gggCTGCCAACTGGCGGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGAcgc           >  2:945980/1‑149 (MQ=255)
                                                                                                                                              ccgccAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGa        >  4:117807/1‑149 (MQ=255)
                                                                                                                                               cgccAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGaa       >  2:837069/1‑149 (MQ=255)
                                                                                                                                                gccAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGaaa      >  1:1040904/1‑149 (MQ=255)
                                                                                                                                                    gTTGGCAGCCCCAGGATTTTTCCGCTAAATGACTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAattat  >  2:70627/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTAT  >  NC_000913/2656243‑2656539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: