Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,666,972 C→T 6.2% W292* (TGG→TAG)  hcaT ← putative 3‑phenylpropionic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,666,9720CT 6.2% 293.0 / 6.1 93W292* (TGG→TAG) hcaTputative 3‑phenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base C (47/40);  new base T (3/3);  total (50/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

GATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCA  >  NC_000913/2666824‑2667120
                                                                                                                                                    |                                                                                                                                                    
gATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAAcc                                                                                                                                                      >  1:786348/1‑149 (MQ=255)
 aTAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCa                                                                                                                                                     >  3:621320/1‑149 (MQ=255)
   aGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGACAGACCGTGAAGGTGCCGCAATGCAGAATTTTCACCACTATCAACCACg                                                                                                                                                   >  4:911278/1‑149 (MQ=255)
    gCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACgg                                                                                                                                                  <  2:769797/149‑1 (MQ=255)
        tACTGCCGCTCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAAc                                                                                                                                              <  3:911278/149‑1 (MQ=255)
        tACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACg                                                                                                                                             >  1:869946/1‑149 (MQ=255)
             ccgccCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGt                                                                                                                                         >  1:639143/1‑149 (MQ=255)
              cgccCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTa                                                                                                                                        <  3:752836/149‑1 (MQ=255)
              cgccCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTa                                                                                                                                        <  1:679567/149‑1 (MQ=255)
                cccATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGtt                                                                                                                                     <  2:869946/149‑1 (MQ=255)
                 ccATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGtt                                                                                                                                     <  1:1048850/149‑1 (MQ=255)
                 ccATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGtt                                                                                                                                     >  4:847209/1‑149 (MQ=255)
                      gcgACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTcc                                                                                                                                >  1:117393/1‑149 (MQ=255)
                        gACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACTACGGCGTTGCCGTGGTTgatagtg                                                                                                                              <  1:474220/149‑7 (MQ=255)
                            gCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAAt                                                                                                                          <  3:974323/149‑1 (MQ=255)
                              agagTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGc                                                                                                                        >  3:856121/1‑149 (MQ=255)
                              agagTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGc                                                                                                                        <  4:621320/149‑1 (MQ=255)
                              agagTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGc                                                                                                                        >  2:679494/1‑149 (MQ=255)
                                agTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGccc                                                                                                                      >  2:1067149/1‑149 (MQ=255)
                                  tAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCa                                                                                                                    >  1:886275/1‑149 (MQ=255)
                                    aaCCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAgc                                                                                                                  <  2:917031/149‑1 (MQ=255)
                                        gCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCAc                                                                                                              <  2:786348/149‑1 (MQ=255)
                                         ccTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACt                                                                                                             >  1:588383/1‑149 (MQ=255)
                                            gTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTAcg                                                                                                          >  4:383337/1‑149 (MQ=255)
                                             tAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTAcgc                                                                                                         <  2:208101/149‑1 (MQ=255)
                                              aaaCGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTAcgcc                                                                                                        >  1:41219/1‑149 (MQ=255)
                                              aaaCGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTAcgcc                                                                                                        <  1:864214/149‑1 (MQ=255)
                                              aaaCGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTAcgcc                                                                                                        <  4:688461/149‑1 (MQ=255)
                                               aaCGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTc                                                                                                                                 >  4:525935/1‑123 (MQ=255)
                                               aaCGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTc                                                                                                                                 <  3:525935/123‑1 (MQ=255)
                                                      gACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCg                                                                                                >  2:638758/1‑149 (MQ=255)
                                                       aCTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGc                                                                                               >  3:207498/1‑149 (MQ=255)
                                                        cTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGcc                                                                                              <  3:383337/149‑1 (MQ=255)
                                                        cTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGcc                                                                                              >  2:415923/1‑149 (MQ=255)
                                                        cTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGcc                                                                                              <  1:166047/149‑1 (MQ=255)
                                                         ttCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCg                                                                                             <  2:397135/149‑1 (MQ=255)
                                                         ttCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCg                                                                                             >  2:530242/1‑149 (MQ=255)
                                                         ttCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCg                                                                                             >  4:1058462/1‑149 (MQ=255)
                                                         ttCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCg                                                                                             >  3:82892/1‑149 (MQ=255)
                                                                cccTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCaa                                                                                      >  3:214771/1‑149 (MQ=255)
                                                                 ccTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAAc                                                                                     <  2:773113/149‑1 (MQ=255)
                                                                  cTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACa                                                                                    <  2:462988/149‑1 (MQ=255)
                                                                  cTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACa                                                                                    <  2:117393/149‑1 (MQ=255)
                                                                   tGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAg                                                                                   >  2:138145/1‑149 (MQ=255)
                                                                       gAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAAGAGCCGCAATGGAGAATTTGCACCACTATCAACCACGGCACCGCCGTAGTTGCTCCCATAATGCGCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCata                                                                               >  2:27415/1‑149 (MQ=255)
                                                                             caATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGt                                                                         >  1:668884/1‑149 (MQ=255)
                                                                               atatAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGc                                                                       >  4:943182/1‑149 (MQ=255)
                                                                                   aaCGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACTACGGCGTTGCCGTGGTTga                                                                                                                                   <  1:247798/85‑2 (MQ=38)
                                                                                   aaCGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACTACGGCGTTGCCGTGGTTga                                                                                                                                   >  4:503589/1‑84 (MQ=38)
                                                                                   aaCGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACTACGGCGTTGCCGTGGTTga                                                                                                                                   >  2:247798/1‑84 (MQ=38)
                                                                                   aaCGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACTACGGCGTTGCCGTGGTTga                                                                                                                                   <  3:503589/85‑2 (MQ=38)
                                                                                      gCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACaa                                                                <  1:638758/149‑1 (MQ=255)
                                                                                      gCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACaa                                                                >  3:618894/1‑149 (MQ=255)
                                                                                       cATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAc                                                               <  1:311760/149‑1 (MQ=255)
                                                                                          ggcggcCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAcggc                                                            <  4:571337/149‑1 (MQ=255)
                                                                                          ggcggcCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAcggc                                                            <  1:415923/149‑1 (MQ=255)
                                                                                           gcggcCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAcggcg                                                           <  3:943182/149‑1 (MQ=255)
                                                                                                cAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGaaaa                                                      <  2:1007491/149‑1 (MQ=255)
                                                                                                 aGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAg                                                     >  4:114134/1‑149 (MQ=255)
                                                                                                 aGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAg                                                     >  4:996122/1‑149 (MQ=255)
                                                                                                  ggTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGt                                                    <  4:445678/149‑1 (MQ=255)
                                                                                                  ggTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGt                                                    >  3:339359/1‑149 (MQ=255)
                                                                                                     ggCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTtta                                                 <  1:803952/149‑1 (MQ=255)
                                                                                                        aGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttatta                                              >  3:456390/1‑149 (MQ=255)
                                                                                                        aGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttatta                                              <  3:996122/149‑1 (MQ=255)
                                                                                                        aGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttatta                                              <  3:114134/149‑1 (MQ=255)
                                                                                                        aGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAACATATCGCGTGCACTACAACGGCGGAAAAGTttatta                                              <  1:641148/149‑1 (MQ=255)
                                                                                                                  ggTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCaa                                    >  1:535932/1‑149 (MQ=255)
                                                                                                                   gTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCaaa                                   <  3:341698/149‑1 (MQ=255)
                                                                                                                   gTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCaaa                                   <  3:1058462/149‑1 (MQ=255)
                                                                                                                        gCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATaa                              <  2:846425/149‑1 (MQ=255)
                                                                                                                        gCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATaa                              <  4:613250/149‑1 (MQ=255)
                                                                                                                         cAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAAt                             <  1:1067149/149‑1 (MQ=255)
                                                                                                                           aTGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCaa                                                                                      <  1:979061/90‑1 (MQ=255)
                                                                                                                           aTGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCaa                                                                                      >  2:979061/1‑90 (MQ=255)
                                                                                                                            tGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTAATTACTCAGCGCAAAGATAATGAc                          >  2:719420/1‑149 (MQ=255)
                                                                                                                                gAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTcc                      >  3:310543/1‑149 (MQ=255)
                                                                                                                                     tGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGccac                 <  2:535932/149‑1 (MQ=255)
                                                                                                                                       caccacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGccacca               >  2:94813/1‑149 (MQ=255)
                                                                                                                                        accacTATGAACTACGGCAACGCCGTAGTTGCTCCCCTAATGCCCCAGCGCCCTAGGCCGCACATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGATTATTACTCAGCGCAAAAATAACGACTTCCGCcaccac              >  4:949105/1‑149 (MQ=255)
                                                                                                                                        accacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCcaccac              >  1:71956/1‑149 (MQ=255)
                                                                                                                                        accacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCcaccac              >  3:58734/1‑149 (MQ=255)
                                                                                                                                        accacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCcaccac              >  2:827118/1‑149 (MQ=255)
                                                                                                                                          cacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGc            >  4:243025/1‑149 (MQ=255)
                                                                                                                                             tATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCa         >  2:987581/1‑149 (MQ=255)
                                                                                                                                             tATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCa         >  4:734847/1‑149 (MQ=255)
                                                                                                                                              aTCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAg        >  3:213110/1‑149 (MQ=255)
                                                                                                                                                 aaCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGa     >  3:209872/1‑149 (MQ=255)
                                                                                                                                                 aaCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGa     <  4:213110/149‑1 (MQ=255)
                                                                                                                                                  aCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGAc    >  1:516775/1‑149 (MQ=255)
                                                                                                                                                  aCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGAc    >  4:971207/1‑149 (MQ=255)
                                                                                                                                                  aCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGAc    >  3:911140/1‑149 (MQ=255)
                                                                                                                                                  aCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGAc    <  3:107918/149‑1 (MQ=255)
                                                                                                                                                  aCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGAc    >  3:819045/1‑149 (MQ=255)
                                                                                                                                                    cACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACca  >  1:904245/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCA  >  NC_000913/2666824‑2667120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: